摘要
采用硅胶、凝胶柱色谱方法对白鲜皮(Dictamnus dasycarpus)的化学成分进行分离纯化。从其石油醚部位分离得到了5个化合物,利用IR、MS和NMR等技术对分离得到的化合物进行了结构鉴定。5个化合物的结构分别为:二十二烷醇(1)、2,2-二二苯甲基-3,3-二苯基丙酸乙酯(2)、柠檬苦素(3)、黄柏酮(4)和白鲜碱(5)。其中化合物2为新化合物,化合物1首次从该植物中分离得到。采用PNPG法筛选化合物的α-葡萄糖苷酶抑制活性。结果表明化合物3~5表现出较好的α-葡萄糖苷酶的抑制活性。该结果为白鲜皮的深度开发利用提供了理论依据。
Five compounds were isolated from the petroleum ether extract of Dictamnus dasycarpus using various chromatographic techniques,such as column chromatography over silica gel and Sephadex LH-20. Their structures were elucidated by spectroscopic data( IR, MS, NMR), which were identified as docosanol( 1), ethyl 2, 2-dibenzhydryl-3,3-di-phenylpropionate( 2),limonin( 3),obacunone( 4) and dictamnine( 5). Compound 2 was a new compound and compound 1 was isolated from this plant for the first time. PNPG method was used to determine α-glucosidase inhibitory activity of these compounds. The results indicated that compounds 3-5 possessed stronger inhibitory activities than the positive control of acarbose,which provided a theoretical basis for further development and utilization of Dictamnus dasycarpus.
引文
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