HIF-1α、OPN在直肠癌组织中的表达及其临床意义
|
摘要
探讨缺氧诱导因子α(HIF-1α)、骨桥蛋白(OPN)在直肠癌组织中的表达及其临床意义。选取2014年6月至2017年6月该院收治的直肠癌患者160例,采用免疫组织化学(SP)法检测直肠癌及其癌旁正常组织中HIF-1α、OPN表达情况,分析两者与直肠癌病理特征及生存预后的关系。直肠癌组织中HIF-1α、OPN阳性表达率明显高于癌旁正常组织,差异有统计学意义(P <0. 05);单因素分析结果显示,HIF-1α、OPN在直肠癌组织中的阳性表达与淋巴结转移、Dukes分期、浸润深度有关(P <0. 05),但与性别、年龄、肿瘤直径、分化程度等无关(P> 0. 05);生存曲线分析结果显示,HIF-1α、OPN阳性表达患者1年生存率明显低于HIF-1α、OPN阴性表达患者,差异有统计学意义(P <0. 05)。HIF-1α、OPN与直肠癌的发生发展有关,提示检测两者表达可作为评估直肠癌生物学特性及生存预后的重要指标,值得临床推广。
To discuss the expression of hypoxia-inducible factor-1α( HIF-1α) and osteopontin( OPN) in rectal cancer and its clinical significance,160 patients with rectal cancer were selected from June 2014 to June 2017 in Zhengding hospital; the immunohistochemistry( SP) was used to detect the expression of HIF-1α and OPN in rectal cancer and its adjacent normal tissues. The relationship between the expression of HIF-1α and OPN and the pathological features and survival prognosis of rectal cancer were analyzed. The positive rates of HIF-1α and OPN in rectal cancer tissues were significantly higher than those in adjacent normal tissues and the difference was statistically significant( P < 0. 05). The Univariate analysis results showed that,the positive expressions of HIF-1α and OPN in rectal cancer tissues were related to lymph node metastasis,Dukes stage and depth of invasion( P < 0. 05),but they were not related to sex,age,tumor diameter and degree of differentiation( P > 0. 05). Survival curve analysis results showed that,the 1-year survival rate of patients with positive expression of HIF-1α and OPN were significantly lower than those of patients with negative expression of HIF-1α and OPN and the difference was statistically significant( P < 0. 05). The expression of HIF-1α and OPN was correlated with the occurrence and development of rectal cancer,suggesting that the detection of their expression may be the important index for evaluating the biological characteristics and survival and prognosis of rectal cancer. It is worth for further clinical promotion.
To discuss the expression of hypoxia-inducible factor-1α( HIF-1α) and osteopontin( OPN) in rectal cancer and its clinical significance,160 patients with rectal cancer were selected from June 2014 to June 2017 in Zhengding hospital; the immunohistochemistry( SP) was used to detect the expression of HIF-1α and OPN in rectal cancer and its adjacent normal tissues. The relationship between the expression of HIF-1α and OPN and the pathological features and survival prognosis of rectal cancer were analyzed. The positive rates of HIF-1α and OPN in rectal cancer tissues were significantly higher than those in adjacent normal tissues and the difference was statistically significant( P < 0. 05). The Univariate analysis results showed that,the positive expressions of HIF-1α and OPN in rectal cancer tissues were related to lymph node metastasis,Dukes stage and depth of invasion( P < 0. 05),but they were not related to sex,age,tumor diameter and degree of differentiation( P > 0. 05). Survival curve analysis results showed that,the 1-year survival rate of patients with positive expression of HIF-1α and OPN were significantly lower than those of patients with negative expression of HIF-1α and OPN and the difference was statistically significant( P < 0. 05). The expression of HIF-1α and OPN was correlated with the occurrence and development of rectal cancer,suggesting that the detection of their expression may be the important index for evaluating the biological characteristics and survival and prognosis of rectal cancer. It is worth for further clinical promotion.
引文
[1]刘清,罗永胜,阳卫立,等.miRNA-31在结直肠癌细胞中的表达及其作用机制的研究[J].现代消化及介入诊疗,2017,22(2):180-185.
[2]张靖宇,朱明珍,孙志超,等.IGF-1R和VEGFR2蛋白在结直肠癌组织中的表达及其临床意义[J].中国药房,2017,28(35):4901-4906.
[3]Blirando K,Mintet E,Buard V,et al.Osteopontin knockout does not influence the severity of rectal damage in a preclinical model of radiation proctitis in mice[J].Dig Dis Sci,2015,60(6):1633-1644.
[4]Guo Q,Lan F,Yan X,et al.Hypoxia exposure induced cisplatin resistance partially via activating p53 and hypoxia inducible factor-1αin non-small cell lung cancer A549 cells[J].Oncol Lett,2018,16(1):801-808.
[5]邓丹玲,崔艳梅,廖雯婷,等.FOXC2在结直肠癌中的表达及意义[J].诊断病理学杂志,2016,23(4):272-275.
[6]马丽,于淑霞,秦旭.HIF-1α与HIF-2α在胃癌中的表达及临床意义[J].现代肿瘤医学,2017,25(22):3635-3639.
[7]Tanam M,Alokta Ishika,Bhossain M,et al.A meta-analysis of hypoxia inducible factor 1-alpha(HIF1A)gene polymorphisms:association with cancers[J].J Geophysical Res Oceans,2015,3(29):226-236.
[8]张俊玲,汪步海,沈雁,等.特异性抑制乏氧诱导因子-1α表达的siRNA筛选[J].药物生物技术,2014,21(2):115-117.
[9]Yamada S,Utsunomiya T,Morine Y,et al.Expressions of hypoxiainducible factor-1 and epithelial cell adhesion molecule are linked with aggressive local recurrence of hepatocellular carcinoma after radio frequency ablation therapy[J].Ann Surg Oncol,2014,21(3):436-442.
[10]刘双立,刘立根,刘冬红,等.骨桥蛋白、神经性-钙粘附素和MMP-9在结直肠癌中的表达及意义[J].中华普外科手术学杂志(电子版),2016,10(3):251-254.
[11]Zduniak K,Ziolkowski P,Ahlin C,et al.Nuclear osteopontin-c is a prognostic breast cancer marker[J].Br J Cancer,2015,112(4):729-738.
[12]刘莹,蒋敦科.骨桥蛋白和组织多肽特异性抗原在胃癌发生发展中的变化及临床意义[J].临床和实验医学杂志,2015,14(4):278-280.
[13]Bandopadhyay M,Bulbule A,Butti R,et al.Osteopontin as a therapeutic target for cancer[J].Expert Opin Ther Targets,2014,18(8):883-895.
[14]汪长健,杨关根,丁菁,等.Glil和OPN在结直肠癌组织中的表达及其临床意义[J].医学研究杂志,2014,43(6):135-137.
[15]Lin S,Lai H,Qin Y,et al.Thymidine phosphorylase and hypoxiainducible factor 1-αexpression in clinical stage II/III rectal cancer:association with response to neoadjuvant chemoradiation therapy and prognosis[J].Int J Clin Exp Pathol,2015,8(9):10680-10688.
[2]张靖宇,朱明珍,孙志超,等.IGF-1R和VEGFR2蛋白在结直肠癌组织中的表达及其临床意义[J].中国药房,2017,28(35):4901-4906.
[3]Blirando K,Mintet E,Buard V,et al.Osteopontin knockout does not influence the severity of rectal damage in a preclinical model of radiation proctitis in mice[J].Dig Dis Sci,2015,60(6):1633-1644.
[4]Guo Q,Lan F,Yan X,et al.Hypoxia exposure induced cisplatin resistance partially via activating p53 and hypoxia inducible factor-1αin non-small cell lung cancer A549 cells[J].Oncol Lett,2018,16(1):801-808.
[5]邓丹玲,崔艳梅,廖雯婷,等.FOXC2在结直肠癌中的表达及意义[J].诊断病理学杂志,2016,23(4):272-275.
[6]马丽,于淑霞,秦旭.HIF-1α与HIF-2α在胃癌中的表达及临床意义[J].现代肿瘤医学,2017,25(22):3635-3639.
[7]Tanam M,Alokta Ishika,Bhossain M,et al.A meta-analysis of hypoxia inducible factor 1-alpha(HIF1A)gene polymorphisms:association with cancers[J].J Geophysical Res Oceans,2015,3(29):226-236.
[8]张俊玲,汪步海,沈雁,等.特异性抑制乏氧诱导因子-1α表达的siRNA筛选[J].药物生物技术,2014,21(2):115-117.
[9]Yamada S,Utsunomiya T,Morine Y,et al.Expressions of hypoxiainducible factor-1 and epithelial cell adhesion molecule are linked with aggressive local recurrence of hepatocellular carcinoma after radio frequency ablation therapy[J].Ann Surg Oncol,2014,21(3):436-442.
[10]刘双立,刘立根,刘冬红,等.骨桥蛋白、神经性-钙粘附素和MMP-9在结直肠癌中的表达及意义[J].中华普外科手术学杂志(电子版),2016,10(3):251-254.
[11]Zduniak K,Ziolkowski P,Ahlin C,et al.Nuclear osteopontin-c is a prognostic breast cancer marker[J].Br J Cancer,2015,112(4):729-738.
[12]刘莹,蒋敦科.骨桥蛋白和组织多肽特异性抗原在胃癌发生发展中的变化及临床意义[J].临床和实验医学杂志,2015,14(4):278-280.
[13]Bandopadhyay M,Bulbule A,Butti R,et al.Osteopontin as a therapeutic target for cancer[J].Expert Opin Ther Targets,2014,18(8):883-895.
[14]汪长健,杨关根,丁菁,等.Glil和OPN在结直肠癌组织中的表达及其临床意义[J].医学研究杂志,2014,43(6):135-137.
[15]Lin S,Lai H,Qin Y,et al.Thymidine phosphorylase and hypoxiainducible factor 1-αexpression in clinical stage II/III rectal cancer:association with response to neoadjuvant chemoradiation therapy and prognosis[J].Int J Clin Exp Pathol,2015,8(9):10680-10688.