金银忍冬鲜果实提取物的GC-MS分析
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摘要
采用索氏提取法,正己烷为溶剂,得到金银忍冬鲜果实提取物。通过气相色谱-质谱(GC-MS)法分析金银忍冬鲜果实提取物的挥发性成分。研究结果表明金银忍冬鲜果实提取物得率为0.77%。金银忍冬鲜果实提取物主要由烷烃类(35.75%)、酯类(27.34%)、酮类(10.89%)、酚类(1.76%)、醇类(0.73%)等化合物组成。GC含量最高的化合物是二十四烷(21.74%),其次为苯甲酸-2,6-双[(三甲基甲硅烷)氧基]-三甲基甲硅烷酯(13.06%)。2,4-双(1,1-二甲基乙基)-苯酚(1.76%),具有抑菌、杀虫活性,为抗氧化剂的重要中间体。
The fresh fruit extracts of Lonicera maackii was obtained by Soxhlet extraction method with hexane as solvent. The volatile components of fresh fruits extracts of L. maackii was analyzed by gas chromatography-mass spectrometry( GC-MS) method. The results show that yield of fresh fruits extracts of L. maackii is 0. 77%. Fresh fruit extracts of L. maackii are mainly composed of alkanes( 35. 75%),esters( 27. 34%),ketones( 10. 89%),phenols( 1. 76%),alcohols( 0. 73%) and other compounds. The highest GC content compound istetracosane( 21. 74%),secondly is benzoic acid-2,6-bis[( trimethylsilyl) oxy]-trimethylsilyl ester( 13. 06%). 2,4-bis( 1,1-dimethylethyl)-phenol( 1. 76%) with antibacterial and insecticidal activity,is an important intermediate of antioxidant.
The fresh fruit extracts of Lonicera maackii was obtained by Soxhlet extraction method with hexane as solvent. The volatile components of fresh fruits extracts of L. maackii was analyzed by gas chromatography-mass spectrometry( GC-MS) method. The results show that yield of fresh fruits extracts of L. maackii is 0. 77%. Fresh fruit extracts of L. maackii are mainly composed of alkanes( 35. 75%),esters( 27. 34%),ketones( 10. 89%),phenols( 1. 76%),alcohols( 0. 73%) and other compounds. The highest GC content compound istetracosane( 21. 74%),secondly is benzoic acid-2,6-bis[( trimethylsilyl) oxy]-trimethylsilyl ester( 13. 06%). 2,4-bis( 1,1-dimethylethyl)-phenol( 1. 76%) with antibacterial and insecticidal activity,is an important intermediate of antioxidant.
引文
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[4]NEERAJ K,BIKRAM S,PAMITA B,et al.Biflavonoids from Lonicera japonica[J].Phytochemistry,2005,66(23):2740-2744.
[5]严雪龙,孟爱平,濮社班.金银花抗炎免疫活性研究进展[J].中国野生植物资源,2016,35(2):41-44.
[6]苏慧.金银忍冬果实中环烯醚萜类活性成分的研究[D].长春:吉林大学,2007.
[7]袁升洁.金银忍冬果实中环烯醚萜类化合物的提取分离与结构鉴定[D].长春:吉林大学,2007.
[8]柴多武.金银忍冬果实抗肿瘤物质的筛选[D].兰州:兰州大学,2010.
[9]王玉莉,杨立,罗国安,等.金银忍冬果实非环烯醚萜苷类化学成分研究[J].天然产物研究与开发,2007,19(1):51-54.
[10]王广树,周小平,袁升杰,等.金银忍冬果实中化学成分的研究[J].中国药物化学杂志,2010,20(3):211-213.
[11]青梅,王素巍,杨慧,等.金银忍冬茎和果实中绿原酸的提取及含量测定[J].内蒙古农业大学学报(自然科学版),2011,32(1):281-284.
[12]姬晓灵,雍建平,汪岭.金银忍冬果实中挥发性成分的GCMS分析[J].光谱实验室,2011,28(6):3010-3012.
[13]李金英,刘洪章,刘树英,等.2种忍冬果实挥发物质组成[J].东北林业大学学报,2015,43(7):133-135.
[14]周宝利,李娜,刘双双,等.2,4-二叔丁基苯酚对番茄叶霉病及幼苗生长的影响[J].生态学杂志,2013,32(5):1203-1207.
[15]王再强,吴毅歆,周红,等.2,4-二叔丁基苯酚对秀丽隐杆线虫的毒杀作用[J].农药,2014,53(4):298-300.
[16]张玉.不同抗氧化剂的抗氧化特性及蛋鸡日粮应用效果比较研究[D].杨凌:西北农林科技大学,2016.
[17]范琛.天然抗氧化剂在文冠果油中抗氧化效果的研究[D].北京:北京林业大学,2016.