薄层扫描法测定新疆不同产地新疆紫草中β,β’-二甲基丙烯酰阿卡宁的含量
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摘要
目的测定新疆不同产地新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量,对药材质量进行初步评价。方法样品经超声提取,点样于高效硅胶G薄层板上,以环己烷-甲苯-乙酸乙酯-甲酸(6∶4∶0.5∶0.4)为展开剂上行展开,在520nm波长进行扫描检测。结果 14个产地新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量为8.215~33.65mg/g,博乐产新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量最高(33.65mg/g),塔什库尔干产新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量最低(8.215mg/g),含量均大于《中国药典》规定。结论采用薄层扫描法测定新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量灵敏度高,准确性好,简便,快速。
Objective To determine the content ofβ,β'-dimelhylacrylalkannin using TLC scanning method taking preliminary stock of the quality of Arnebia euchromafrom different Areas of Xinjian.MethodsThe samples collected were extracted by the method of ultrasonic extraction and the silica gel G thin layer plate was adopted in the determination with cyclohexane:toluene:ethyl acetate:formic acid(6∶4∶0.5∶0.4)used as developer.The quantitative analysis was conducted by single wavelength scanning at 520 nm.Results The content range ofβ,β'-dimelhylacrylalkannin in the root of Arnebia euchromafrom 14 areas of Xinjiang is 8.215~33.65 mg/g.in terms of the content of the herb producing bole was highest,up to33.65mg/g.The lowest was Taxkorgan.which was 8.215mg/g.All of them were higher than its requirements in Chinese Pharmacopoeia.Conclusion TLC Scanning Method was shown to be simple,accurate and reproducible in the determination of content ofβ,β'-dimelhylacrylalkannin in the root of Arnebia euchroma.
Objective To determine the content ofβ,β'-dimelhylacrylalkannin using TLC scanning method taking preliminary stock of the quality of Arnebia euchromafrom different Areas of Xinjian.MethodsThe samples collected were extracted by the method of ultrasonic extraction and the silica gel G thin layer plate was adopted in the determination with cyclohexane:toluene:ethyl acetate:formic acid(6∶4∶0.5∶0.4)used as developer.The quantitative analysis was conducted by single wavelength scanning at 520 nm.Results The content range ofβ,β'-dimelhylacrylalkannin in the root of Arnebia euchromafrom 14 areas of Xinjiang is 8.215~33.65 mg/g.in terms of the content of the herb producing bole was highest,up to33.65mg/g.The lowest was Taxkorgan.which was 8.215mg/g.All of them were higher than its requirements in Chinese Pharmacopoeia.Conclusion TLC Scanning Method was shown to be simple,accurate and reproducible in the determination of content ofβ,β'-dimelhylacrylalkannin in the root of Arnebia euchroma.
引文
[1]《中华本草》编纂委员会.中华本草[M].上海:上海科学技术出版社,1999:525.
[2]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工出版社,2005:238-239.
[3]买尔旦·马合木提,马冉,古丽仙·胡加.市售新疆紫草药材的质量考察[J].新疆医科大学学报,2015,38(5):581-585.
[4]詹志来,胡峻,刘谈,等.紫草化学成分与药理活性研究进展[J].中国中药杂志,2015,40(21):4127-4135.
[5]AI KH.Studies on the naphthaquinone constituents of Onosma Confertum W.W.Smith and quantitative determination of Shikonin[J].Acta Botanica Sinica,1989,31(7):549-553.
[6]FU SL,SHANG TM,XIAO PG.Analysis of naphthaquinone pigments in Chinese medicinal“zicao”,Arnebia euchroma[J].Acta Pharmaceutica Sinica,1984,19(12):921.
[7]LI C,FEI J,FUKUSHI Y.Inactivate function of the propionylshikonin for TMV[J].J China Agricultural University,1997,22(5):47-50.
[8]崔晓秋,刘云刚.HPLC测定新疆紫草中紫草素的含量及提取方法的优化[J].中国实验方剂学杂志,2014,20(16):94-97.
[9]周化更,刘晓华,富志军.新疆紫草中紫草素的提取方法研究[J].海峡药学,2015(7):30-32.
[10]宋艳华,孙晖,张爱华,等.紫草的研究进展[J].中医药学报,2013,41(4):123-125.
[11]陈征,陈文明,任卫琼,等.不同市场紫草相关质量的比较研究[J].中国当代医药,2015(10):7-9.
[12]周健,唐雪梅,王聪,等.新疆紫草根与茎残基部分化学成分及含量的比较研究[J].中药材,2012,35(3):361-366.
[13]姜林,李晓瑾,贾晓光.新疆紫草HPLC指纹图谱的研究[J].中成药,2005,27(11):1241-1244.
[14]彭金年,程庚金生,李丹丹,等.薄层扫描法测定不同提取方法枇杷叶中齐墩果酸含量[J].辽宁中医药大学学报,2015,32(6):43-45.
[15]郭隆钢,孙帅,谢培山,等.数码扫描薄层色谱影像直接测定黄连提取物中4种生物碱含量[J].中药新药与临床药理,2015,26(1):92-95.
[16]谭亮,冀恬,曹静亚,等.双波长薄层扫描法测定不同来源枸杞子中甜菜碱的含量[J].天然产物研究与开发,2014,26(3):388-391.
[2]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工出版社,2005:238-239.
[3]买尔旦·马合木提,马冉,古丽仙·胡加.市售新疆紫草药材的质量考察[J].新疆医科大学学报,2015,38(5):581-585.
[4]詹志来,胡峻,刘谈,等.紫草化学成分与药理活性研究进展[J].中国中药杂志,2015,40(21):4127-4135.
[5]AI KH.Studies on the naphthaquinone constituents of Onosma Confertum W.W.Smith and quantitative determination of Shikonin[J].Acta Botanica Sinica,1989,31(7):549-553.
[6]FU SL,SHANG TM,XIAO PG.Analysis of naphthaquinone pigments in Chinese medicinal“zicao”,Arnebia euchroma[J].Acta Pharmaceutica Sinica,1984,19(12):921.
[7]LI C,FEI J,FUKUSHI Y.Inactivate function of the propionylshikonin for TMV[J].J China Agricultural University,1997,22(5):47-50.
[8]崔晓秋,刘云刚.HPLC测定新疆紫草中紫草素的含量及提取方法的优化[J].中国实验方剂学杂志,2014,20(16):94-97.
[9]周化更,刘晓华,富志军.新疆紫草中紫草素的提取方法研究[J].海峡药学,2015(7):30-32.
[10]宋艳华,孙晖,张爱华,等.紫草的研究进展[J].中医药学报,2013,41(4):123-125.
[11]陈征,陈文明,任卫琼,等.不同市场紫草相关质量的比较研究[J].中国当代医药,2015(10):7-9.
[12]周健,唐雪梅,王聪,等.新疆紫草根与茎残基部分化学成分及含量的比较研究[J].中药材,2012,35(3):361-366.
[13]姜林,李晓瑾,贾晓光.新疆紫草HPLC指纹图谱的研究[J].中成药,2005,27(11):1241-1244.
[14]彭金年,程庚金生,李丹丹,等.薄层扫描法测定不同提取方法枇杷叶中齐墩果酸含量[J].辽宁中医药大学学报,2015,32(6):43-45.
[15]郭隆钢,孙帅,谢培山,等.数码扫描薄层色谱影像直接测定黄连提取物中4种生物碱含量[J].中药新药与临床药理,2015,26(1):92-95.
[16]谭亮,冀恬,曹静亚,等.双波长薄层扫描法测定不同来源枸杞子中甜菜碱的含量[J].天然产物研究与开发,2014,26(3):388-391.