导入海岛棉染色体片段对新疆陆地棉纤维品质性状的影响
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摘要
为了研究导入海岛棉染色体片段对新疆陆地棉纤维品质的影响,利用陆地棉和海岛棉染色体片段置换系杂交回交构建了2个BC2F1群体。利用SSR标记筛选鉴定BC2F1群体中含有海岛棉染色体片段的阳性单株,分别对2个BC2F1群体中阳性单株与非阳性单株的纤维品质性状进行比较分析。结果显示,在新陆早33号×CSSL-122的BC2F1群体中,阳性植株纤维长度显著高于非阳性植株;在金垦69-2×CSSL-122的BC2F1群体中,阳性植株纤维长度极显著高于非阳性植株,阳性植株的纤维强度、伸长率显著高于非阳性植株。说明导入外源海岛棉染色体片段,显著或极显著地提高了新疆陆地棉纤维长度、强度等性状。
Molecular markers of SSR were employed to accurately identify individual plants carrying the chromosome segments from Gossypium barbadense in two BC2F1populations( Xinluzao 33×CSSL-122) and( Jinken 69-2×CSSL-122).And the fiber quality traits between positive plants and non-positive plants in two BC2F1 populations were compared. The fibers of positive plants were significantly longer than those of non-positive plants. And fiber strengths and elongation rates of positive plants were higher than those of non-positive plants as well. It was suggested that introgressing alien chromosome segments from G. barbadense into Xinjiang upland cotton had significantly improved its fiber quality.
Molecular markers of SSR were employed to accurately identify individual plants carrying the chromosome segments from Gossypium barbadense in two BC2F1populations( Xinluzao 33×CSSL-122) and( Jinken 69-2×CSSL-122).And the fiber quality traits between positive plants and non-positive plants in two BC2F1 populations were compared. The fibers of positive plants were significantly longer than those of non-positive plants. And fiber strengths and elongation rates of positive plants were higher than those of non-positive plants as well. It was suggested that introgressing alien chromosome segments from G. barbadense into Xinjiang upland cotton had significantly improved its fiber quality.
引文
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[15]马麒,宿俊吉,陈红,等.利用海岛棉染色体片段置换系改良新陆早45号纤维品质性状的研究[J].棉花学报,2013,25(6):496-502.
[16]张军,武耀廷,郭旺珍,等.棉花微卫星标记的PAGE/银染快速检测[J].棉花学报,2000,12(5):267-269.
[17]ZHANG J,GUO W Z,ZHANG T Z.Molecular linkage map of allotetraploid cotton(Gossypium hirsutum L.×Gossypium barbadense L.)with a haploid population[J].Theoretical and Applied Genetics,2002,105(8):1166-1174.
[18]CULP T W,HARREL D,KERR T.Some genetic implications in the transfer of high fiber strength genes to upland cotton[J].Crop Sci,1979,19:481-484.
[19]STEPHENS S G.The cytogenetics of speciation in Gossypium.I.Selective elimination of the donor parent genotype in interspecific backcrosses[J].Genetics,1949,34:627-637.
[20]MCKENZIE W H.Fertility relationships among interspecific hybrid progenies of Gossypium[J].Crop Sci,1970,10:571-574.
[21]张金凤,石玉真,梁燕,等.陆地棉染色体片段代换系(BC5F3和BC5F3∶4)产量和纤维品质性状表现的评价[J].植物遗传资源学报,2012,13(5):773-781.
[22]WANG P,ZHU Y,SONG X,et al.Inheritance of long staple fiber quality traits of Gossypium barbadense in G.hirsutum background using CSILs[J].Theoretical and Applied Genetics,2012,124(8):1415-1428.
[23]GUO W Z,CAI C P,Wang C B,et al.A microsatellite-based,gene-rich linkage map reveals genome structure,function and evolution in cotton[J].Genetics,2007,5(176):527-541.
[24]XU Z,KOHEL R J,SONG G L,et al.Gene-rich islands for fiber development in the cotton genome[J].Genomics,2008,92:173-183.
[2]钱能.陆地棉遗传多样性与育种目标性状基因(QTL)的关联分析[D].南京:南京农业大学,2009.
[3]杨泽茂,李骏智,李爱国,等.利用高代回交和分子标记辅助选择构建棉花染色体片段代换系[J].分子植物育种,2009,7(2):233-241.
[4]WANG P,DING Y Z,LU Q X,et al.Development of G.barbadense L.chromosome segment substitution lines with the background of upland cotton standard lines TM-1[J].Chinese Science Bulletin,2008,53(9):1065-1069.
[5]KOHEL R J,ENDREZZI J E,WHITE T G.An evaluation of Gossypium barbadense chromosomes 6 and 17 in the G.hirsutum L[J].Crop Sci,1977,17:404-406.
[6]王志伟.分子标记辅助选择构建棉花种间单片段代换系及其遗传评价[D].武汉:华中农业大学,2009.
[7]冯常辉.陆地棉邯郸208背景的海岛棉Pima 90染色体片段导入系的构建及评价[D].武汉:华中农业大学,2009.
[8]梁燕.早熟陆地棉染色体片段代换系的构建及QTL定位初探[D].安阳:中国农业科学院棉花研究所,2010.
[9]央付,苑冬冬,胡文静,等.陆地棉背景下海岛棉第18染色体片段置换系的培育及相关农艺性状QTL定位[J].作物学报,2013,39(1):21-28.
[10]李夕梅.棉花种间导入系的构建、新型标记的开发与高密度遗传图谱的构建[D].武汉:华中农业大学,2013.
[11]张天真.一种改良棉花纤维长度、纤维强度和马克隆值的分子育种方法:中国,CN102229982A[P].2011-11-02.
[12]CAO Z B,WANG P,ZHU X,et al.SSR marker-assisted improvement of fiber qualities in Gossypium hirsutum using G.barbadense introgression lines[J].Theoretical and Applied Genetics,2013,127(3):587-594.
[13]马晓梅,李保成,李生秀,等.早熟陆地棉新陆早32号与33号的选育[J].种子,2009,28(5):114-116.
[14]PATERSON A H,BRUBAKER C L,WENDEL J F.A rapid method for extraction of cotton(Gossypium spp.)genomic DNA suitable for RFLP or PCR analysis[J].Plant Molecular Biology Reporter,1993,11(2):122-127.
[15]马麒,宿俊吉,陈红,等.利用海岛棉染色体片段置换系改良新陆早45号纤维品质性状的研究[J].棉花学报,2013,25(6):496-502.
[16]张军,武耀廷,郭旺珍,等.棉花微卫星标记的PAGE/银染快速检测[J].棉花学报,2000,12(5):267-269.
[17]ZHANG J,GUO W Z,ZHANG T Z.Molecular linkage map of allotetraploid cotton(Gossypium hirsutum L.×Gossypium barbadense L.)with a haploid population[J].Theoretical and Applied Genetics,2002,105(8):1166-1174.
[18]CULP T W,HARREL D,KERR T.Some genetic implications in the transfer of high fiber strength genes to upland cotton[J].Crop Sci,1979,19:481-484.
[19]STEPHENS S G.The cytogenetics of speciation in Gossypium.I.Selective elimination of the donor parent genotype in interspecific backcrosses[J].Genetics,1949,34:627-637.
[20]MCKENZIE W H.Fertility relationships among interspecific hybrid progenies of Gossypium[J].Crop Sci,1970,10:571-574.
[21]张金凤,石玉真,梁燕,等.陆地棉染色体片段代换系(BC5F3和BC5F3∶4)产量和纤维品质性状表现的评价[J].植物遗传资源学报,2012,13(5):773-781.
[22]WANG P,ZHU Y,SONG X,et al.Inheritance of long staple fiber quality traits of Gossypium barbadense in G.hirsutum background using CSILs[J].Theoretical and Applied Genetics,2012,124(8):1415-1428.
[23]GUO W Z,CAI C P,Wang C B,et al.A microsatellite-based,gene-rich linkage map reveals genome structure,function and evolution in cotton[J].Genetics,2007,5(176):527-541.
[24]XU Z,KOHEL R J,SONG G L,et al.Gene-rich islands for fiber development in the cotton genome[J].Genomics,2008,92:173-183.