血清维生素K1水平与绝经后女性腰椎骨密度相关性分析
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摘要
目的探讨绝经后女性骨密度与血清维生素K1水平之间的相关性。方法使用标准化的酶联免疫吸附试验(ELISA)试剂盒测量46例绝经后骨质疏松女性和30名绝经后健康对照女性的血清维生素K1水平。检测腰椎(1～4)的骨密度(bone mineral density, BMD)。结果绝经后骨质疏松女性组血清维生素K1水平明显低于正常对照组(P<0.05),绝经后骨质疏松女性血清维生素K1浓度与腰椎BMD呈正相关(R=0.545,P=0.003);绝经后正常对照组血清维生素K1浓度与腰椎BMD呈正相关(R=0.513,P=0.009)。维生素K1对骨质疏松症的诊断敏感性和特异性分别为91%和98%(截止值:0.853 ng/mL);维生素K1的ROC曲线下面积(AUC)为0.985,奇数比为18.88。结论维生素K1与诊断绝经后骨质疏松症呈负相关。
Objective To investigate the correlation between bone mineral density and serum vitamin K1 levels in postmenopausal women. Methods Serum levels of vitamin K1 were measured in 46 postmenopausal osteoporotic women, and in 30 postmenopausal healthy control women using a standardized Enzyme-Linked Immune Sorbent Assay(ELISA) kit. Bone mineral density(BMD) was assessed at the lumbar spine L_(1-4). Results The mean serum vitamin K1 level was significantly lower in the postmenopausal osteoporosis group than in the normal control group(P<0.05), and serum vitamin K1 concentration was positively correlated with lumbar spine BMD among postmenopausal osteoporotic women(R=0.545,P=0.003), and in postmenopausal healthy controls(R=0.513,P=0.009). Diagnostic sensitivity and specificity of vitamin K1 for osteoporosis were 91% and 98%, respectively(cut-off value: 0.853 ng/mL). The area under the ROC curve(AUC) value for vitamin K1 was 0.985, and the odd ratio result was 18.88. Conclusion Our result suggest that vitamin K1 is negatively correlated with the diagnosis of postmenopausal osteoporosis.
Objective To investigate the correlation between bone mineral density and serum vitamin K1 levels in postmenopausal women. Methods Serum levels of vitamin K1 were measured in 46 postmenopausal osteoporotic women, and in 30 postmenopausal healthy control women using a standardized Enzyme-Linked Immune Sorbent Assay(ELISA) kit. Bone mineral density(BMD) was assessed at the lumbar spine L_(1-4). Results The mean serum vitamin K1 level was significantly lower in the postmenopausal osteoporosis group than in the normal control group(P<0.05), and serum vitamin K1 concentration was positively correlated with lumbar spine BMD among postmenopausal osteoporotic women(R=0.545,P=0.003), and in postmenopausal healthy controls(R=0.513,P=0.009). Diagnostic sensitivity and specificity of vitamin K1 for osteoporosis were 91% and 98%, respectively(cut-off value: 0.853 ng/mL). The area under the ROC curve(AUC) value for vitamin K1 was 0.985, and the odd ratio result was 18.88. Conclusion Our result suggest that vitamin K1 is negatively correlated with the diagnosis of postmenopausal osteoporosis.
引文
[1] Lorentzon M, Cummings SR. Osteoporosis: the evolution of a diagnosis[J]. Journal of Internal Medicine, 2015,277(6): 650-661.
[2] Czeczuk A, Hukwieliczuk E, Michalska A, et al. The effect of menopause on bone tissue in former swimmers and in non-athletes[J]. Advances in Clinical & Experimental Medicine Official Organ Wroclaw Medical University, 2012,21(5): 645-652.
[3] Hoesel CHM, Wehr U, Wenisch S, et al. Diagnosis of Osteoporosis with Vitamin K as a New Biochemical Marker[J]. Vitamins & Hormones, 2008,78: 417-434.
[4] Shikano K, Kaneko K, Kawazoe M, et al. Efficacy of Vitamin K2 for Glucocorticoid-induced Osteoporosis in Patients with Systemic Autoimmune Diseases[J]. Internal Medicine, 2016,55(15): 1997-2003.
[5] Bügel S. Vitamin K and bone health in adult humans[J]. Vitamins & Hormones-advances in Research & Applications, 2008,78: 393-416.
[6] Ivey JL, Baylink DJ. Postmenopausal osteoporosis: Proposed roles of defective coupling and estrogen deficiency[J]. Metabolic Bone Disease & Related Research, 1981,3(1): 3-7.
[7] Palermo A, Tuccinardi D, D'Onofrio L, et al.Vitamin K and osteoporosis: Myth or reality?[J] Metabolism-clinical & Experimental, 2017,70: 57-71.
[8] Macdonald HM, Mcguigan FE, Lanham-New SA, et al. Vitamin K1 intake is associated with higher bone mineral density and reduced bone resorption in early postmenopausal Scottish women: no evidence of gene-nutrient interaction with apolipoprotein E polymorphisms[J]. American Journal of Clinical Nutrition, 2008,87(5): 1513-1520.
[9] Tsugawa N, Shiraki M, Suhara Y, et al. Low plasma phylloquinone concentration is associated with high incidence of vertebral fracture in Japanese women[J]. Journal of Bone & Mineral Metabolism, 2008,26 (1): 79-85.
[10] Finnes TE, Lofthus CM, Meyer HE, et al. A combination of low serum concentrations of vitamins K 1 and D is associated with increased risk of hip fractures in elderly Norwegians: a NOREPOS study[J]. Osteoporosis International, 2016,27 (4): 1645-1652.
[11] Fujita Y, Iki M, Tamaki J, et al. Association between vitamin K intake from fermented soybeans, natto, and bone mineral density in elderly Japanese men: the Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) study[J]. Osteoporosis International, 2012,23 (2): 705-714.
[12] Ikeda Y, Iki M, Morita A, et al. Intake of fermented soybeans, natto, is associated with reduced bone loss in postmenopausal women: Japanese Population-Based Osteoporosis (JPOS) Study[J]. Journal of Nutrition, 2006,136 (5): 1323-1328.
[13] Hodges SJ, Akesson K, Vergnaud P, et al. Circulating levels of vitamins K1 and K2 decreased in elderly women with hip fracture[J]. Journal of Bone & Mineral Research the Official Journal of the American Society for Bone & Mineral Research, 1993,8 (10): 1241-1245.
[14] Jaghsi S, Hammoud T, Haddad S. Relation Between Circulating Vitamin K1 and Osteoporosis in the Lumbar Spine in Syrian Post-Menopausal Women[J]. Open Rheumatology Journal, 2018,12 (1): 1-9.
[15] Mi SK, Eun SK, Cheong MS. Dietary intake of vitamin K in relation to bone mineral density in Korea adults: The Korea National Health and Nutrition Examination Survey (2010-2011)[J]. Journal of Clinical Biochemistry & Nutrition, 2015,57 (3): 223-227.
[16] Hamidi MS, Cheung AM. Vitamin K and musculoskeletal health in postmenopausal women[J]. Molecular Nutrition & Food Research, 2014,58 (8): 1647-1657.
[17] Fang Y, Hu C, Tao X, et al. Effect of vitamin K on bone mineral density: a meta-analysis of randomized controlled trials[J]. Journal of Bone & Mineral Metabolism, 2012,30 (1): 60-68.
[2] Czeczuk A, Hukwieliczuk E, Michalska A, et al. The effect of menopause on bone tissue in former swimmers and in non-athletes[J]. Advances in Clinical & Experimental Medicine Official Organ Wroclaw Medical University, 2012,21(5): 645-652.
[3] Hoesel CHM, Wehr U, Wenisch S, et al. Diagnosis of Osteoporosis with Vitamin K as a New Biochemical Marker[J]. Vitamins & Hormones, 2008,78: 417-434.
[4] Shikano K, Kaneko K, Kawazoe M, et al. Efficacy of Vitamin K2 for Glucocorticoid-induced Osteoporosis in Patients with Systemic Autoimmune Diseases[J]. Internal Medicine, 2016,55(15): 1997-2003.
[5] Bügel S. Vitamin K and bone health in adult humans[J]. Vitamins & Hormones-advances in Research & Applications, 2008,78: 393-416.
[6] Ivey JL, Baylink DJ. Postmenopausal osteoporosis: Proposed roles of defective coupling and estrogen deficiency[J]. Metabolic Bone Disease & Related Research, 1981,3(1): 3-7.
[7] Palermo A, Tuccinardi D, D'Onofrio L, et al.Vitamin K and osteoporosis: Myth or reality?[J] Metabolism-clinical & Experimental, 2017,70: 57-71.
[8] Macdonald HM, Mcguigan FE, Lanham-New SA, et al. Vitamin K1 intake is associated with higher bone mineral density and reduced bone resorption in early postmenopausal Scottish women: no evidence of gene-nutrient interaction with apolipoprotein E polymorphisms[J]. American Journal of Clinical Nutrition, 2008,87(5): 1513-1520.
[9] Tsugawa N, Shiraki M, Suhara Y, et al. Low plasma phylloquinone concentration is associated with high incidence of vertebral fracture in Japanese women[J]. Journal of Bone & Mineral Metabolism, 2008,26 (1): 79-85.
[10] Finnes TE, Lofthus CM, Meyer HE, et al. A combination of low serum concentrations of vitamins K 1 and D is associated with increased risk of hip fractures in elderly Norwegians: a NOREPOS study[J]. Osteoporosis International, 2016,27 (4): 1645-1652.
[11] Fujita Y, Iki M, Tamaki J, et al. Association between vitamin K intake from fermented soybeans, natto, and bone mineral density in elderly Japanese men: the Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) study[J]. Osteoporosis International, 2012,23 (2): 705-714.
[12] Ikeda Y, Iki M, Morita A, et al. Intake of fermented soybeans, natto, is associated with reduced bone loss in postmenopausal women: Japanese Population-Based Osteoporosis (JPOS) Study[J]. Journal of Nutrition, 2006,136 (5): 1323-1328.
[13] Hodges SJ, Akesson K, Vergnaud P, et al. Circulating levels of vitamins K1 and K2 decreased in elderly women with hip fracture[J]. Journal of Bone & Mineral Research the Official Journal of the American Society for Bone & Mineral Research, 1993,8 (10): 1241-1245.
[14] Jaghsi S, Hammoud T, Haddad S. Relation Between Circulating Vitamin K1 and Osteoporosis in the Lumbar Spine in Syrian Post-Menopausal Women[J]. Open Rheumatology Journal, 2018,12 (1): 1-9.
[15] Mi SK, Eun SK, Cheong MS. Dietary intake of vitamin K in relation to bone mineral density in Korea adults: The Korea National Health and Nutrition Examination Survey (2010-2011)[J]. Journal of Clinical Biochemistry & Nutrition, 2015,57 (3): 223-227.
[16] Hamidi MS, Cheung AM. Vitamin K and musculoskeletal health in postmenopausal women[J]. Molecular Nutrition & Food Research, 2014,58 (8): 1647-1657.
[17] Fang Y, Hu C, Tao X, et al. Effect of vitamin K on bone mineral density: a meta-analysis of randomized controlled trials[J]. Journal of Bone & Mineral Metabolism, 2012,30 (1): 60-68.