体外模拟消化条件下岩藻黄素产品抗氧化活性的变化
|
摘要
目的评价岩藻黄素产品在消化系统中的稳定性及变化规律。方法本文考察了在体外消化模拟状态下岩藻黄素产品中岩藻黄素含量变化,并采用测定其对DPPH自由基清除能力、铁离子还原能力(ferricion reducing antioxidant power, FRAP)与氧自由基吸收能力(oxygen radical absorbance capacity, ORAC)这3种抗氧化指标来评价岩藻黄素产品的抗氧化能力变化。结果随着人工模拟胃肠消化过程时间的增加,岩藻黄素产品中岩藻黄素的含量呈下降趋势,但岩藻黄素微囊粉较岩藻黄素油悬液对岩藻黄素表现出良好的包埋与保护作用。在胃肠消化过程中,岩藻黄素微囊粉与油悬液对DPPH自由基清除能力、FRAP铁离子还原能力与ORAC氧自由基吸收能力等抗氧化活性指标均有所降低,其中岩藻黄素微囊粉较油悬液稳定,表现出较好的抗氧化活性。结论岩藻黄素微囊粉产品比油悬液产品在消化体系中具有更好的稳定性。
Objective To evaluate the stability and variation of fucoxanthin products in the digestive system. Methods The changes of fucoxanthin content in fucoxanthin products under simulated digestion conditions in vitro were investigated in this paper. The antioxidant activities of fucoxanthin products were evaluated by 3 kinds of antioxidant indexes including DPPH free radical scavenging capacity, ferric reducing antioxidant power(FRAP) and oxygen radical absorbance capacity(ORAC). Results The content of fucoxanthin in the products decreased with the increase of simulated gastrointestinal digestion time, but fucoxanthin microcapsule powder showed better embedding and protection effect than fucoxanthin oil suspension. During gastrointestinal digestion, the antioxidant activities of fucoxanthin microcapsule powder and oil suspension, including DPPH free radical scavenging capacity, iron ion reduction capacity and oxygen free radical absorption capacity, were decreased, but fucoxanthin microcapsule powder was more stable than oil suspension, which showed better antioxidant activity. Conclusion Fucoxanthin microcapsule powder product has better stability than oil suspension product in the digestive system.
Objective To evaluate the stability and variation of fucoxanthin products in the digestive system. Methods The changes of fucoxanthin content in fucoxanthin products under simulated digestion conditions in vitro were investigated in this paper. The antioxidant activities of fucoxanthin products were evaluated by 3 kinds of antioxidant indexes including DPPH free radical scavenging capacity, ferric reducing antioxidant power(FRAP) and oxygen radical absorbance capacity(ORAC). Results The content of fucoxanthin in the products decreased with the increase of simulated gastrointestinal digestion time, but fucoxanthin microcapsule powder showed better embedding and protection effect than fucoxanthin oil suspension. During gastrointestinal digestion, the antioxidant activities of fucoxanthin microcapsule powder and oil suspension, including DPPH free radical scavenging capacity, iron ion reduction capacity and oxygen free radical absorption capacity, were decreased, but fucoxanthin microcapsule powder was more stable than oil suspension, which showed better antioxidant activity. Conclusion Fucoxanthin microcapsule powder product has better stability than oil suspension product in the digestive system.
引文
[1]李丹彤,陈国栋,张玲丽,等.裙带菜岩藻黄素的提取分离及对人肝癌细胞HepG2的抑制作用研究[J].辽宁师范大学学报,2012,35(3):383-389.Li DT,Chen GD,Zhang LL,et al.Extraction and separation of fucoxanthin from wakame and its inhibitory effect on human hepatoma cell HepG2[J].J Liaoning Normal Univ,2012,35(3):383-389.
[2]Sachindra NM,Sato E,Maeda H,et al.Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites[J].J Agric Food Chem,2007,55(21):8516-8522.
[3]Riccioni G.Marine carotenoids and oxidative stress[J].Marin Drug,2012,10(1):116-118.
[4]Tanaka T,Shnimizu M,Moriwaki H.Cancer chemoprevention by carotenoids[J].Molecules,2012,17(3):3202-3242.
[5]Kim KN,Heo SJ,Kang SM,et al.Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a Ros-mediated Bcl-xL pathway[J].Toxicol Vitr,2010,24(6):1648-1654.
[6]Bharathiraja K,Hari BL,Vijayaprakash S,et al.Fucoxanthin,a marine carotenoid protects cadmium-induced oxidative renal dysfunction in rats[J].Biomed Prev Nutr,2013,3(3):201-207.
[7]Ekmekcioglu C.A physiological approach for preparing and conducting intestinal bioavailability studies using experimental systems[J].Food Chem,2002,76(2):225-230.
[8]Hur SJ,Lim BO,Decker E,et al.In vitro human digestion models for food applications[J].Food Chem,2011,125(1):1-12.
[9]Rodriguez-Amaya DB.Quantitative analysis,in vitro assessment of bioavailability and antioxidant activity of food carotenoids-A review[J].JFood Composit Anal,2010,23(7):726-740.
[10]Garrett DA,Failla ML,Sarama RJ.Development of an in vitro digestion method to assess carotenoid bioavailability from meals[J].J Agric Food Chem,1999,47(10):4301-4309.
[11]Hedren E,Diaz V,Svanberg U.Estimation of carotenoid accessibility from carrots determined by an in vitro digestion method[J].Eur J Clin Nutr,2002,56(5):425.
[12]王丽.海藻类胡萝卜素结构鉴定及微胶囊化的研究[D].大连:大连海洋大学,2015.Wang L.Structural identification and microencapsulation of seaweed carotenoids[D].Dalian:Dalian Ocean University,2015.
[13]张欣,赵新淮.几种多酚化合物抗氧化性的不同化学评价及相关性分析[J].食品科学,2008,29(10):85-89.Zhang X,Zhao XH.Different chemical evaluation and correlation analysis of antioxidant activity of several polyphenol compounds[J].Food Sci,2008,29(10):85-89.
[14]熊双丽,卢飞,史敏娟,等.DPPH自由基清除活性评价方法在抗氧化剂筛选中的研究进展[J].食品工业科技,2012,(8):380-383.Xiong SL,Lu F,Shi MJ,et al.Research progress in the evaluation of DPPH free radical scavenging activity in antioxidant screening[J].Sci Technol Food Ind,2012,(8):380-383.
[15]恽祥惠,邢丽娜,李云,等.建立基于96孔板检测的FRAP法和DPPH法及其在橘核抗氧化活性研究中的应用[J].时珍国医国药,2014,5(5):1275-1278.Qi XH,Xing LN,Li Y,et al.Establishment of FRAP method and DPPHmethod based on 96-well plate detection and its application in the study of antioxidant activity of orange nucleus[J].Lishizhen Med Mater Med Res,2014,5(5):1275-1278.
[16]陈玉霞,刘建华,林峰,等.DPPH和FRAP法测定41种中草药抗氧化活性[J].实验室研究与探索,2011,30(6):11-14.Chen YX,Liu JH,Lin F,et al.Determination of antioxidant activity of 41Chinese herbal medicines by DPPH and FRAP[J].Res Explorat Lab,2011,30(6):11-14.
[17]续洁琨,姚新生,栗原博.抗氧化能力指数(ORAC)测定原理及应用[J].中国药理学通报,2006,22(8):1015-1021.Xu JK,Yao XS,Li YB.Principles and applications of oxidation resistance index(ORAC)measurement[J].Chin Pharmacol Bull,2006,22(8):1015-1021.
[18]Huang D,Ou B,Hampsch-Woodill M,et al.Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated beta-cyclodextrin as the solubility enhancer[J].J Agric Food Chem,2002,50(7):1815-1821.
[2]Sachindra NM,Sato E,Maeda H,et al.Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites[J].J Agric Food Chem,2007,55(21):8516-8522.
[3]Riccioni G.Marine carotenoids and oxidative stress[J].Marin Drug,2012,10(1):116-118.
[4]Tanaka T,Shnimizu M,Moriwaki H.Cancer chemoprevention by carotenoids[J].Molecules,2012,17(3):3202-3242.
[5]Kim KN,Heo SJ,Kang SM,et al.Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a Ros-mediated Bcl-xL pathway[J].Toxicol Vitr,2010,24(6):1648-1654.
[6]Bharathiraja K,Hari BL,Vijayaprakash S,et al.Fucoxanthin,a marine carotenoid protects cadmium-induced oxidative renal dysfunction in rats[J].Biomed Prev Nutr,2013,3(3):201-207.
[7]Ekmekcioglu C.A physiological approach for preparing and conducting intestinal bioavailability studies using experimental systems[J].Food Chem,2002,76(2):225-230.
[8]Hur SJ,Lim BO,Decker E,et al.In vitro human digestion models for food applications[J].Food Chem,2011,125(1):1-12.
[9]Rodriguez-Amaya DB.Quantitative analysis,in vitro assessment of bioavailability and antioxidant activity of food carotenoids-A review[J].JFood Composit Anal,2010,23(7):726-740.
[10]Garrett DA,Failla ML,Sarama RJ.Development of an in vitro digestion method to assess carotenoid bioavailability from meals[J].J Agric Food Chem,1999,47(10):4301-4309.
[11]Hedren E,Diaz V,Svanberg U.Estimation of carotenoid accessibility from carrots determined by an in vitro digestion method[J].Eur J Clin Nutr,2002,56(5):425.
[12]王丽.海藻类胡萝卜素结构鉴定及微胶囊化的研究[D].大连:大连海洋大学,2015.Wang L.Structural identification and microencapsulation of seaweed carotenoids[D].Dalian:Dalian Ocean University,2015.
[13]张欣,赵新淮.几种多酚化合物抗氧化性的不同化学评价及相关性分析[J].食品科学,2008,29(10):85-89.Zhang X,Zhao XH.Different chemical evaluation and correlation analysis of antioxidant activity of several polyphenol compounds[J].Food Sci,2008,29(10):85-89.
[14]熊双丽,卢飞,史敏娟,等.DPPH自由基清除活性评价方法在抗氧化剂筛选中的研究进展[J].食品工业科技,2012,(8):380-383.Xiong SL,Lu F,Shi MJ,et al.Research progress in the evaluation of DPPH free radical scavenging activity in antioxidant screening[J].Sci Technol Food Ind,2012,(8):380-383.
[15]恽祥惠,邢丽娜,李云,等.建立基于96孔板检测的FRAP法和DPPH法及其在橘核抗氧化活性研究中的应用[J].时珍国医国药,2014,5(5):1275-1278.Qi XH,Xing LN,Li Y,et al.Establishment of FRAP method and DPPHmethod based on 96-well plate detection and its application in the study of antioxidant activity of orange nucleus[J].Lishizhen Med Mater Med Res,2014,5(5):1275-1278.
[16]陈玉霞,刘建华,林峰,等.DPPH和FRAP法测定41种中草药抗氧化活性[J].实验室研究与探索,2011,30(6):11-14.Chen YX,Liu JH,Lin F,et al.Determination of antioxidant activity of 41Chinese herbal medicines by DPPH and FRAP[J].Res Explorat Lab,2011,30(6):11-14.
[17]续洁琨,姚新生,栗原博.抗氧化能力指数(ORAC)测定原理及应用[J].中国药理学通报,2006,22(8):1015-1021.Xu JK,Yao XS,Li YB.Principles and applications of oxidation resistance index(ORAC)measurement[J].Chin Pharmacol Bull,2006,22(8):1015-1021.
[18]Huang D,Ou B,Hampsch-Woodill M,et al.Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated beta-cyclodextrin as the solubility enhancer[J].J Agric Food Chem,2002,50(7):1815-1821.