NTF3基因与公猪繁殖性能相关性研究进展
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摘要
神经营养因子3(neurotrophin 3,NTF3)是一种由靶细胞分泌合成并经神经突起逆向转运至胞体而发挥作用的小分子蛋白质,主要在神经系统的发育和生理功能维持中发挥重要作用。但近年来的研究表明,NTF3及其受体与睾丸的发育、成熟和精子发生过程等密切相关,同时,NTF3基因对公猪性机能发育、性成熟、生精调节等方面具有重要调控功能。本文简介NTF3的分子结构、分布、受体及作用机制、生物学功能,重点阐述NTF3基因的启动子、转录因子及其与公猪繁殖性能相关性的研究状况,以期为今后开展更多、更深入的科学研究提供参考。
NTF3(neurotrophin 3) is a small-molecule protein secreted by the target cells and transported back to the cell body through the neuritis, playing an important role in the development of nervous system and maintenance of physiological functions. However, recent studies have shown that NTF3 and its receptors are closely related to testicular development and maturation, and spermatogenesis. Meanwhile, the NTF3 gene has important regulatory functions in the development of sexual function, sexual maturity and spermatogenesis of boars. This article describes the molecular structure, distribution, receptors and mechanism, biological functions of NTF3, and focuses on the promoter, transcription factors of the NTF3 gene and its correlation with reproductive performance of boar, in to the hope of providing the reference for related scientific research in the future.
NTF3(neurotrophin 3) is a small-molecule protein secreted by the target cells and transported back to the cell body through the neuritis, playing an important role in the development of nervous system and maintenance of physiological functions. However, recent studies have shown that NTF3 and its receptors are closely related to testicular development and maturation, and spermatogenesis. Meanwhile, the NTF3 gene has important regulatory functions in the development of sexual function, sexual maturity and spermatogenesis of boars. This article describes the molecular structure, distribution, receptors and mechanism, biological functions of NTF3, and focuses on the promoter, transcription factors of the NTF3 gene and its correlation with reproductive performance of boar, in to the hope of providing the reference for related scientific research in the future.
引文
[1] Mccoard S A, Wise T H, Ford J J. Endocrine and molecular influences on testicular development in Meishan and White Composite boars[J].J Endocrinol, 2003, 178(3):405-416.
[2] Borg K E, Lunstra D D, Christenson R K. Semen characteristics, testicular size, and reproductive hormone concentrations in mature duroc, meishan, fengjing, and minzhu boars[J].Biol Reprod, 1993, 49(3):515-521.
[3] Benn K,Passos M, Jayaram A, et al. Association between neurotrophin 4 and long-chain polyunsaturated fatty acid levels in mid-trimester amniotic fluid[J].Reprod Sci, 2014, 21(11):1395-1400.
[4] Ernfors P, Ibáňez C F, Ebendal T, et al. Molecular clonig and neurotrophic activites of a protein with structural similarities to nerve growth factor: development and topographical expression in the brain[J].Proc Natl Acad Sci U S A, 1990, 87(14):5454-5458.
[5] Ernfors P, Wetmore C, Olson L, et al. Identification of cells in rat brain and perioheral tissues expressing mRNA for members of the nerve growth factor family[J].Neuron, 1990, 5(4):511-526.
[6] 杨宏,伯音,周长满,等.神经营养素在小鼠脾的定位研究[J].解剖学报,1998,29(4):374-378.
[7] Salehi A H, Roux P P, Kubu C J, et al. NRAGE, a novel mage protein, interacts with the p75 neurotrophin receptor and failitates nerve growth factor-dependent apoptosis[J].Neuron, 2000, 27(2):279-288.
[8] Maisonpiere P C, Belluscio L, Squinto S, et al. Neurotrophin-3: a neurotrophic factor related to NGF and BDNF[J].Science, 1990, 247 (4949 Pt 1):1446-1451.
[9] Zhou X F, Rush R A. Location of neurotrophin-3-like immmunoreactivity in the rat central nevous system[J].Brain Res, 1994, 643(1-2):162-172.
[10] Perez-Navarro E,Acberch J, Neven I, et al. Brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4/5 differentially regulate the ohenotype and prevent degenerative changes in striatal projection neurons after excitoxicity in vivo[J].Neuroscience, 1999, 91(4):1257-1264.
[11] 芭帕婉.抑制素和脑源性神经营养因子在猪卵泡中的表达[D].长春:吉林大学,2012.
[12] 张志,李爱民.神经营养因子-3研究进展[J].临床神经外科杂志,2013,10(3):191-192.
[13] 石慧,李建远.真核生物启动子的鉴定方法[J].中外医学研究,2012,10(8):154-155.
[14] Smale S T, Kadonaga J T. The RNA polymerase II core promoter[J].Annu Rev Biochem, 2003, 72(1):449-479.
[15] Butler J E F, Kadonaga J T. The RNA polymerase II core promoter: a key component in the regulation of gene expression[J].Genes Dev, 2002, 16(20):2583-2592.
[16] 张艳馥,沙伟.转录因子概述[J].生物学教学,2009 (10):7-8.
[17] Werner T. Models for prediction and recognition of eukaryotic promoters[J].Mamm Genome, l999, 10(2):l68-175.
[18] Sauer F, Tjian R. Mechanisms of transcripltional activation: differences and similarities between yeast, Drosophila, and man[J].Curr Opin Genet Dev, 1997, 7(2):176-181.
[19] 陈哲,雷明明,于建宁,等.猪RELMβ基因启动子区克隆及序列分析[J].江苏农业学报,2015,31(5):1060-1064.
[20] Capel B, Albrecht K H, Washburn L L, et al. Migration of mesonephric cells into the mammalian gonad depends on Sry[J].Mech Dev, 1999, 84(1-2):127-131.
[21] Brennan J, Capel B. One tissue, two fates: molecular genetic events that underlie testis versus ovary development[J].Nat Rev Genet, 2004, 5(7):509-521.
[22] Cupp A S, Kim G H, Skinner M K. Expression and action of neurotropin-3 and nerve growth factor in embryonic and early postnatal rat testis development[J].Biol Reprod, 2000, 63(6):1617-1628.
[23] Russo M A, Giustizieri M L, Favale A, et al. Spatiotemporal patterns of expression of neurotrophins and neurotrophin receptors in mice suggest functional roles in testicular and epididymal morphogenesis[J].Biol Reprod, 1999, 61(4):1123-1132.
[24] Robinson L L, Townsend J, Anderson R A. The human fetal testis is a site of expression of neurotrophins and their receptors: regulation of the germ cell and peritubular cell population[J].J Clin Endocrinol Metab, 2003, 88(8):3943-3951.
[25] Choi M, Wang S E, Ko S Y, et al. Overexpression of human GATA-1 and GATA-2 interferes with spine formation and produces depressive behavior in rats[J]. PLoS One, 2014, 9(10):e109253.
[26] Ko L J, Engel J D. DNA-binding specificities of the GATA transcription factor family[J].Mol Cell Biol, 1993, 13(7):4011-4022.
[27] Hsieh Y H, Liu J Y. Supression of tumorigenicity of human hepatocellular carcinoma cells by antisense oligonucleotide MZF-1[J].Chin J Physiol, 2007, 50(1):217-225.
[28] Chelbi S T, Wilson M, Veillard A C, et al. Genetic and epigenetic mechanisms collaborate to control SERPINA3 expression and its association with placental diseases[J].Hum Mol Genet, 2012, 21(9):1968-1978.
[29] 马强,秦洋,夏青娟,等.4个猪种IGF-1基因核心启动子区的克隆及生物信息学分析[J].长春:吉林农业大学学报,2013,35(5):587-590.
[30] Bouwman P, Philipsen S. Regulation of the activity of Spl-related transcription factors[J].Mol Cell Endocrinol, 2002, 195(1):27-38.
[31] Safe S,Abdelrahim M. Sp transcription factor family and its role in cancer[J].Eur J Cancer, 2005, 41(16):2438-2448.
[32] Lui W Y, Sze K L, Lee W M. Nectin-2 expression in testicular cells is controlled via the functional cooperation between transcription factors of the Sp1, CREB, and AP-1families[J].J Cell Physiol, 2006, 207(1):144-157.
[33] Jokela T A, Makkonen K M, Oikari S, et al. Cellular content of UDP-N-acetylhexosamines controls hyaluronan synthase 2 expre-ssion and correlates with O-linked N-acetylglucosamine modification of transcription factors YY1 and SP1[J].J Biol Chem, 2011, 286(38):33632-33640.
[34] Dufour J M, Rajotte R V, Korbutt G S. Development of an in vivo model to study testicular morphogenesis[J].J Androl, 2002, 23(5):635-644.
[35] Gassei K, Schlatt S. Testicular morphogenesis: comparison of in vivo and in vitro models to study male gonadal development[J].Ann N Y Acad Sci, 2007, 1120(1):152-167.
[36] Turner L M, Chuong E B, Hoekstra H E. Comparative analysis of testis protein evolution in rodents[J].Genetics, 2008, 179(4):2075-2089.
[37] Ding H, Luo Y, Liu M, et al. Histological and transcriptome analyses of testes from Duroc and Meishan boars[J].Sci Rep, 2016, 6: 20758.
[38] Barrionuevo F J, Burgos M, Scherer G, et al. Genes promoting and disturbing testis development[J].Histol Histopathol, 2012, 27(11):1361-1383.
[39] Cupp A S, Uzumcu M, Skinner M K. Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination[J].Biol Reprod, 2003, 68(6):2033-2037.
[40] Levine E, Cupp A S, Skinner M K. Role of neurotropins in rat embryonic testis morphogenesis (cord formation)[J].Biol Reprod, 2000, 62(1):132-142.
[41] Clement T M, Bhandari R K, Sadler-Riggleman I, et al. SRY directly regulates the neurotrophin 3 promoter during male sex determination and testis development in rats[J].Biol Reprod, 2011, 85(2):277-284.
[42] Parvinen M, Pelto-Huikko M, S?der O. Expression of beta-nerve growth factor and its receptor in rat seminiferous epithelium: specic function at the onset of meiosis[J].J Cell Biol, 1992, 117(3):629-641.
[43] Cupp A S, Tessarollo L, Skinner M K. Testis developmental phenotypes in neurotropin receptor TRKA and TRKC null mutations: role in formation of seminiferous cords and germ cell survival[J].Biol Reprod, 2002, 66(6):1838-1845.
[44] Guyonnet B, Marot G, Dacheux J L, et al. The adult boar testicular and epididymal transcriptomes[J].BMC Genomics, 2009, 10(1):369.
[2] Borg K E, Lunstra D D, Christenson R K. Semen characteristics, testicular size, and reproductive hormone concentrations in mature duroc, meishan, fengjing, and minzhu boars[J].Biol Reprod, 1993, 49(3):515-521.
[3] Benn K,Passos M, Jayaram A, et al. Association between neurotrophin 4 and long-chain polyunsaturated fatty acid levels in mid-trimester amniotic fluid[J].Reprod Sci, 2014, 21(11):1395-1400.
[4] Ernfors P, Ibáňez C F, Ebendal T, et al. Molecular clonig and neurotrophic activites of a protein with structural similarities to nerve growth factor: development and topographical expression in the brain[J].Proc Natl Acad Sci U S A, 1990, 87(14):5454-5458.
[5] Ernfors P, Wetmore C, Olson L, et al. Identification of cells in rat brain and perioheral tissues expressing mRNA for members of the nerve growth factor family[J].Neuron, 1990, 5(4):511-526.
[6] 杨宏,伯音,周长满,等.神经营养素在小鼠脾的定位研究[J].解剖学报,1998,29(4):374-378.
[7] Salehi A H, Roux P P, Kubu C J, et al. NRAGE, a novel mage protein, interacts with the p75 neurotrophin receptor and failitates nerve growth factor-dependent apoptosis[J].Neuron, 2000, 27(2):279-288.
[8] Maisonpiere P C, Belluscio L, Squinto S, et al. Neurotrophin-3: a neurotrophic factor related to NGF and BDNF[J].Science, 1990, 247 (4949 Pt 1):1446-1451.
[9] Zhou X F, Rush R A. Location of neurotrophin-3-like immmunoreactivity in the rat central nevous system[J].Brain Res, 1994, 643(1-2):162-172.
[10] Perez-Navarro E,Acberch J, Neven I, et al. Brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4/5 differentially regulate the ohenotype and prevent degenerative changes in striatal projection neurons after excitoxicity in vivo[J].Neuroscience, 1999, 91(4):1257-1264.
[11] 芭帕婉.抑制素和脑源性神经营养因子在猪卵泡中的表达[D].长春:吉林大学,2012.
[12] 张志,李爱民.神经营养因子-3研究进展[J].临床神经外科杂志,2013,10(3):191-192.
[13] 石慧,李建远.真核生物启动子的鉴定方法[J].中外医学研究,2012,10(8):154-155.
[14] Smale S T, Kadonaga J T. The RNA polymerase II core promoter[J].Annu Rev Biochem, 2003, 72(1):449-479.
[15] Butler J E F, Kadonaga J T. The RNA polymerase II core promoter: a key component in the regulation of gene expression[J].Genes Dev, 2002, 16(20):2583-2592.
[16] 张艳馥,沙伟.转录因子概述[J].生物学教学,2009 (10):7-8.
[17] Werner T. Models for prediction and recognition of eukaryotic promoters[J].Mamm Genome, l999, 10(2):l68-175.
[18] Sauer F, Tjian R. Mechanisms of transcripltional activation: differences and similarities between yeast, Drosophila, and man[J].Curr Opin Genet Dev, 1997, 7(2):176-181.
[19] 陈哲,雷明明,于建宁,等.猪RELMβ基因启动子区克隆及序列分析[J].江苏农业学报,2015,31(5):1060-1064.
[20] Capel B, Albrecht K H, Washburn L L, et al. Migration of mesonephric cells into the mammalian gonad depends on Sry[J].Mech Dev, 1999, 84(1-2):127-131.
[21] Brennan J, Capel B. One tissue, two fates: molecular genetic events that underlie testis versus ovary development[J].Nat Rev Genet, 2004, 5(7):509-521.
[22] Cupp A S, Kim G H, Skinner M K. Expression and action of neurotropin-3 and nerve growth factor in embryonic and early postnatal rat testis development[J].Biol Reprod, 2000, 63(6):1617-1628.
[23] Russo M A, Giustizieri M L, Favale A, et al. Spatiotemporal patterns of expression of neurotrophins and neurotrophin receptors in mice suggest functional roles in testicular and epididymal morphogenesis[J].Biol Reprod, 1999, 61(4):1123-1132.
[24] Robinson L L, Townsend J, Anderson R A. The human fetal testis is a site of expression of neurotrophins and their receptors: regulation of the germ cell and peritubular cell population[J].J Clin Endocrinol Metab, 2003, 88(8):3943-3951.
[25] Choi M, Wang S E, Ko S Y, et al. Overexpression of human GATA-1 and GATA-2 interferes with spine formation and produces depressive behavior in rats[J]. PLoS One, 2014, 9(10):e109253.
[26] Ko L J, Engel J D. DNA-binding specificities of the GATA transcription factor family[J].Mol Cell Biol, 1993, 13(7):4011-4022.
[27] Hsieh Y H, Liu J Y. Supression of tumorigenicity of human hepatocellular carcinoma cells by antisense oligonucleotide MZF-1[J].Chin J Physiol, 2007, 50(1):217-225.
[28] Chelbi S T, Wilson M, Veillard A C, et al. Genetic and epigenetic mechanisms collaborate to control SERPINA3 expression and its association with placental diseases[J].Hum Mol Genet, 2012, 21(9):1968-1978.
[29] 马强,秦洋,夏青娟,等.4个猪种IGF-1基因核心启动子区的克隆及生物信息学分析[J].长春:吉林农业大学学报,2013,35(5):587-590.
[30] Bouwman P, Philipsen S. Regulation of the activity of Spl-related transcription factors[J].Mol Cell Endocrinol, 2002, 195(1):27-38.
[31] Safe S,Abdelrahim M. Sp transcription factor family and its role in cancer[J].Eur J Cancer, 2005, 41(16):2438-2448.
[32] Lui W Y, Sze K L, Lee W M. Nectin-2 expression in testicular cells is controlled via the functional cooperation between transcription factors of the Sp1, CREB, and AP-1families[J].J Cell Physiol, 2006, 207(1):144-157.
[33] Jokela T A, Makkonen K M, Oikari S, et al. Cellular content of UDP-N-acetylhexosamines controls hyaluronan synthase 2 expre-ssion and correlates with O-linked N-acetylglucosamine modification of transcription factors YY1 and SP1[J].J Biol Chem, 2011, 286(38):33632-33640.
[34] Dufour J M, Rajotte R V, Korbutt G S. Development of an in vivo model to study testicular morphogenesis[J].J Androl, 2002, 23(5):635-644.
[35] Gassei K, Schlatt S. Testicular morphogenesis: comparison of in vivo and in vitro models to study male gonadal development[J].Ann N Y Acad Sci, 2007, 1120(1):152-167.
[36] Turner L M, Chuong E B, Hoekstra H E. Comparative analysis of testis protein evolution in rodents[J].Genetics, 2008, 179(4):2075-2089.
[37] Ding H, Luo Y, Liu M, et al. Histological and transcriptome analyses of testes from Duroc and Meishan boars[J].Sci Rep, 2016, 6: 20758.
[38] Barrionuevo F J, Burgos M, Scherer G, et al. Genes promoting and disturbing testis development[J].Histol Histopathol, 2012, 27(11):1361-1383.
[39] Cupp A S, Uzumcu M, Skinner M K. Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination[J].Biol Reprod, 2003, 68(6):2033-2037.
[40] Levine E, Cupp A S, Skinner M K. Role of neurotropins in rat embryonic testis morphogenesis (cord formation)[J].Biol Reprod, 2000, 62(1):132-142.
[41] Clement T M, Bhandari R K, Sadler-Riggleman I, et al. SRY directly regulates the neurotrophin 3 promoter during male sex determination and testis development in rats[J].Biol Reprod, 2011, 85(2):277-284.
[42] Parvinen M, Pelto-Huikko M, S?der O. Expression of beta-nerve growth factor and its receptor in rat seminiferous epithelium: specic function at the onset of meiosis[J].J Cell Biol, 1992, 117(3):629-641.
[43] Cupp A S, Tessarollo L, Skinner M K. Testis developmental phenotypes in neurotropin receptor TRKA and TRKC null mutations: role in formation of seminiferous cords and germ cell survival[J].Biol Reprod, 2002, 66(6):1838-1845.
[44] Guyonnet B, Marot G, Dacheux J L, et al. The adult boar testicular and epididymal transcriptomes[J].BMC Genomics, 2009, 10(1):369.