助阳开郁颗粒的急性毒性研究
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摘要
目的:研究助阳开郁颗粒的急性毒性,为其制剂开发及临床应用提供实验依据。方法:将动物按体重随机分为对照组和4个给药组,对照组灌胃40 mL/kg生理盐水,给药组分别以10 mL/kg、20 mL/kg、30 mL/kg、40 mL/kg的助阳开郁颗粒混悬液灌胃,进行急性毒性实验,计算其半数致死量。结果:小鼠均未出现死亡,与对照组比较,给药组小鼠的行为活动、体重以及解剖后的脏器都未见异常。实验未测出助阳开郁颗粒的半数致死量(LD50)。结论:测得小鼠的最大耐受量为临床成人用量的153.15倍,未见明显毒性反应,助阳开郁颗粒对小鼠的急性毒性甚微,在规定剂量下服用安全可靠。
Objective:To study the acute toxicity of Zhuyang Kaiyu granules,which provided some experimental basis for its clinical application. Methods:The mice were randomly divided into control group and 4 drug groups according to their weight. Then the mice in control group were administered intragastically normal saline according to 40 mL/kg,while the administration groups were given suspension of Zhuyang Kaiyu granules according to 10 mL/kg、20 mL/kg、30 mL/kg、40 mL/kg respectively. The numbers of dead mice was counted and LD50 of experimental drug was culculated. Results:There were no death in all groups. No abnormalities were found in the behavioral activity,body weight and organs indice of the mice in the administration groups. Half of the lethal quantity of Zhuyang Kaiyu granules was not measured in the experiment. Conclusion:The maximum tolerance dosage of the mice was 153.15 times of the clinical adult dosage,and no obvious toxicity was observed. The acute toxicity of Zhuyang Kaiyu granules to mice is very little so it is safe and reliable to take within the prescribed dosage range of the drug.
Objective:To study the acute toxicity of Zhuyang Kaiyu granules,which provided some experimental basis for its clinical application. Methods:The mice were randomly divided into control group and 4 drug groups according to their weight. Then the mice in control group were administered intragastically normal saline according to 40 mL/kg,while the administration groups were given suspension of Zhuyang Kaiyu granules according to 10 mL/kg、20 mL/kg、30 mL/kg、40 mL/kg respectively. The numbers of dead mice was counted and LD50 of experimental drug was culculated. Results:There were no death in all groups. No abnormalities were found in the behavioral activity,body weight and organs indice of the mice in the administration groups. Half of the lethal quantity of Zhuyang Kaiyu granules was not measured in the experiment. Conclusion:The maximum tolerance dosage of the mice was 153.15 times of the clinical adult dosage,and no obvious toxicity was observed. The acute toxicity of Zhuyang Kaiyu granules to mice is very little so it is safe and reliable to take within the prescribed dosage range of the drug.
引文
[1]李晓骄阳,栾永福,孙蓉.附子不同组分对正常小鼠的急性毒性实验比较研究[J].中国药物警戒,2013,10(10):583-587.
[2]王云.黑附子及配伍甘草、干姜对小鼠致死毒性随机平行对照研究[J].实用中医内科杂志,2014,28(4):68-70.
[3]王超婷,李云文,彭微,等.调胃健脾丸最大给药量的实验研究[J].湖南中医杂志,2017,33(1):150-151.
[4]周思思,马增春,梁乾德,等.人参附子不同比例配伍对小鼠急性毒性的影响[J].天津中医药,2013,30(1):43-46.
[5]沈玉巧,林华,邓广海,等.不同炮制工艺附子煎煮液的急性毒性探讨[J].中国实验方剂学杂志,2015,21(12):121-124.
[6]谢晓芳,彭成,易进海,等.附子不同炮制品提取物急性毒性的比较研究[J].中药与临床,2012,3(3):29-33.
[7]刘振东,周维顺,杨永波,等.升血小板冲剂最大给药量的确定及其对血小板减少小鼠的体重和脾脏指数影响[J].中华中医药学刊,2014,32(6):1326-1328.
[8]彭慧,王妍妍,张盛,等.黑顺片不同组分对正常小鼠的急性毒性[J].中成药,2018,40(8):1842-1844.
[9]周子渝,熊永爱,黄勤挽,等.附子不同炮制品及其部位急性毒性研究[J].成都中医药大学学报,2012,35(3):63-65.
[10]李冰洁,沈勇,廖日滔,等.从蛋白质自组装的角度探析甘草附子配伍减毒机制[J].中国中药杂志,2015,40(4):661-666.
[2]王云.黑附子及配伍甘草、干姜对小鼠致死毒性随机平行对照研究[J].实用中医内科杂志,2014,28(4):68-70.
[3]王超婷,李云文,彭微,等.调胃健脾丸最大给药量的实验研究[J].湖南中医杂志,2017,33(1):150-151.
[4]周思思,马增春,梁乾德,等.人参附子不同比例配伍对小鼠急性毒性的影响[J].天津中医药,2013,30(1):43-46.
[5]沈玉巧,林华,邓广海,等.不同炮制工艺附子煎煮液的急性毒性探讨[J].中国实验方剂学杂志,2015,21(12):121-124.
[6]谢晓芳,彭成,易进海,等.附子不同炮制品提取物急性毒性的比较研究[J].中药与临床,2012,3(3):29-33.
[7]刘振东,周维顺,杨永波,等.升血小板冲剂最大给药量的确定及其对血小板减少小鼠的体重和脾脏指数影响[J].中华中医药学刊,2014,32(6):1326-1328.
[8]彭慧,王妍妍,张盛,等.黑顺片不同组分对正常小鼠的急性毒性[J].中成药,2018,40(8):1842-1844.
[9]周子渝,熊永爱,黄勤挽,等.附子不同炮制品及其部位急性毒性研究[J].成都中医药大学学报,2012,35(3):63-65.
[10]李冰洁,沈勇,廖日滔,等.从蛋白质自组装的角度探析甘草附子配伍减毒机制[J].中国中药杂志,2015,40(4):661-666.