甲醛修饰酿酒酵母对铀的吸附研究
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摘要
采用批次实验方法探究了甲醛修饰酿酒酵母对铀的生物吸附过程.结果显示,甲醛修饰能显著提高酵母对铀的吸附能力,其吸附量是同等实验条件下活酵母的6倍,动力学研究表明,达到吸附平衡仅需90 min,并且能较好地符合准二级动力学吸附模型.实验的最佳吸附pH为5.8.Langmuir和Freundlich模型均能用于拟合实验数据且实验结果与Langmuir模型更加吻合.扫描电镜和傅里叶变换红外光谱分析结果表明,甲醛修饰改变了细胞的表面形貌和蛋白结构,使氨基发生了甲基化,羟基形成配位共价键,在酵母吸附铀后,细胞表面不均匀地附着了一层鳞片状的铀沉淀.铀沉淀与络合物的形式多种多样,且与羧酸盐主要络合物为双齿配体结构,甲醛修饰酿酒酵母与铀酰离子相互作用存在着络合,沉淀以及静电吸附等多种机理.
The biosorption of uranium from aqueous solutions in batch by methanal modified Saccharomyces cerevisiae biomass was investigated. Results show that methanal modification could significantly improve the cells sorption capacity which was six times than that of raw cells under the same condition.Kinetic study suggests that the reaction reached an equilibrium within only 90 min and the pseudo-second-order model was well in great correlation with the adsorption behavior. The optimum uranium uptake pH was determined at 5.8. Langmuir and Freundlich equation were applied to fit the experimental data and the Langmuir model was better fit with the results. Scanning electron microscopy( SEM) images and Fourier transform infrared( FTIR) spectrum show that methanal treatment had changed cell surface morphology and protein structure,alcoholic-hydroxylate of amino groups and dative-linked with alcoholic groups. After exposed in uranium solution,the surface of cells was attached with scale-like uranium precipitation unevenly,the uranyl ion complexes and precipitation are multiplicate,and the type of uranyl-carboxylate complexes is mainly bidentate chelating coordination. The complexation,precipitation and electrostatic interaction were the mechanisms of methanal modified yeast-uranium interaction.
The biosorption of uranium from aqueous solutions in batch by methanal modified Saccharomyces cerevisiae biomass was investigated. Results show that methanal modification could significantly improve the cells sorption capacity which was six times than that of raw cells under the same condition.Kinetic study suggests that the reaction reached an equilibrium within only 90 min and the pseudo-second-order model was well in great correlation with the adsorption behavior. The optimum uranium uptake pH was determined at 5.8. Langmuir and Freundlich equation were applied to fit the experimental data and the Langmuir model was better fit with the results. Scanning electron microscopy( SEM) images and Fourier transform infrared( FTIR) spectrum show that methanal treatment had changed cell surface morphology and protein structure,alcoholic-hydroxylate of amino groups and dative-linked with alcoholic groups. After exposed in uranium solution,the surface of cells was attached with scale-like uranium precipitation unevenly,the uranyl ion complexes and precipitation are multiplicate,and the type of uranyl-carboxylate complexes is mainly bidentate chelating coordination. The complexation,precipitation and electrostatic interaction were the mechanisms of methanal modified yeast-uranium interaction.
引文
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Wang T S,Zheng X Y,Wang X Y,et al.2017.Different biosorption mechanisms of Uranium(VI)by live and heat-killed Saccharomyces cerevisiae under environmentally relevant conditions[J].Journal of Environmental Radioactivity,167:92-99
翁诗甫.2010.傅里叶变换红外光谱分析[M].北京:化学工业出版社.377-388
王镜岩,朱圣庚,徐长法.2002.生物化学(第3版)(上册)[M].北京:高等教育出版社.133-135
Zhang C,Malhotra S V,Francis A J.2014.Toxicity of ionic liquids to Clostridium,sp.and effects on uranium biosorption[J].Journal of Hazardous Materials,264(2):246-253
周书葵,娄涛,庞朝辉,等.2011.放射性废水处理技术[M].北京:化学工业出版社.7-8
白静,秦芝,王菊芳,等.2009.粘红酵母对铀的吸附研究[J].光谱学与光谱分析,29(5):1218-1221
Chen J P,Yang L.2006.Study of a heavy metal biosorption onto raw and chemically modified Sargassum sp.via spectroscopic and modeling analysis[J].Langmuir,22(21):8906-8914
陈灿,王建龙.2006.酿酒酵母吸附重金属离子的研究进展[J].中国生物工程杂志,26(1):69-76
Faghihian H,Peyvandi S.2012.Adsorption isotherm for uranyl biosorption by Saccharomyces cerevisiae,biomass[J].Journal of Radioanalytical and Nuclear Chemistry,293(2):463-468
Galichet A,Sockalingum G D,Belarbi A,et al.2001.FTIRspectroscopic analysis of Saccharomyces cerevisiae cell walls:study of an anomalous strain exhibiting a pink-colored cell phenotype[J].FEMS Microbiology Letters,197(2):179-186
G9ksungur Y,Uren S,Güven9 U.2005.Biosorption of cadmium and lead ions by ethanol treated waste baker's yeast biomass[J].Bioresource Technology,96(1):103-109
Iqbal M,Saeed A,Zafar S I.2009.FTIR spectrophotometry,kinetics and adsorption isotherms modeling,ion exchange,and EDX analysis for understanding the mechanism of Cd2+and Pb2+removal by mango peel waste[J].Journal of Hazardous Materials,164(1):161-171
Lu X,Zhou X J,Wang T S.2013.Mechanism of uranium(VI)uptake by Saccharomyces cerevisiae,under environmentally relevant conditions:Batch,HRTEM,and FTIR studies[J].Journal of Hazardous Materials,262(8):297-303
Mukhopadhyay M,Noronha S B,Suraishkumar G K.2007.Kinetic modeling for the biosorption of copper by pretreated Aspergillus niger biomass[J].Bioresource Technology,98(9):1781-1787
Müller K,Brendler V,Foerstendorf H.2008.Aqueous uranium(VI)hydrolysis species characterized by attenuated total reflection Fouriertransform infrared spectroscopy[J].Inorganic Chemistry,47(21):10127-10134
Ruchhoft C C.1949.The possibilities of disposal of radioactive wastes by biological treatment methods[J].Sewage Works Journal,21(5):877-883
Sarri S,Misaelides P,Papanikolaou M,et al.2009.Uranium removal from acidic aqueous solutions by Saccharomyces cerevisiae,Debaryomyces hansenii,Kluyveromyces marxianus and Candida colliculosa[J].Journal of Radioanalytical and Nuclear Chemistry,279(3):709-711
Tsezos M.1983.The role of chitin in uranium adsorption by R.arrhizus[J].Biotechnology and Bioengineering,25(8):2025-2040
Vogel M,Günther A,Rossberg A,et al.2010.Biosorption of U(VI)by the green algae Chlorella vulgaris in dependence of p H value and cell activity[J].Science of the Total Environment,409(2):384-395
Wang J S,Hu X J,Wang J,et al.2010.The tolerance of Rhizopus arrihizus to U(VI)and biosorption behavior of U(VI)onto R.arrihizus[J].Biochemical Engineering Journal,51(1):19-23
Wang J L,Chen C.2006.Biosorption of heavy metals by Saccharomyces cerevisiae:a review[J].Biotechnology Advances,24(5):427-451
Wang T S,Zheng X Y,Wang X Y,et al.2017.Different biosorption mechanisms of Uranium(VI)by live and heat-killed Saccharomyces cerevisiae under environmentally relevant conditions[J].Journal of Environmental Radioactivity,167:92-99
翁诗甫.2010.傅里叶变换红外光谱分析[M].北京:化学工业出版社.377-388
王镜岩,朱圣庚,徐长法.2002.生物化学(第3版)(上册)[M].北京:高等教育出版社.133-135
Zhang C,Malhotra S V,Francis A J.2014.Toxicity of ionic liquids to Clostridium,sp.and effects on uranium biosorption[J].Journal of Hazardous Materials,264(2):246-253
周书葵,娄涛,庞朝辉,等.2011.放射性废水处理技术[M].北京:化学工业出版社.7-8