宣肺平喘方对哮喘TGF-β1、Smad2、Smad7水平的影响研究
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摘要
目的:观察宣肺平喘方对哮喘患者的治疗效果,及对TGF-β1、Smad2、Smad7水平的影响。方法:选取2016年3月至2017年3月广元市第二人民医院收治的哮喘患者80例为研究对象,根据其治疗方式分为对照组和观察组,每组40例,对照组给予常规药物治疗,观察组在此基础上给予宣肺平喘方治疗。观察2组患者的治疗效果,比较2组患者治疗前后肺功能、炎性反应因子、TGF-β1、Smad2、Smad7水平的差异。结果:观察组患者治疗的有效率为97. 50%,明显高于对照组(χ2=5. 000,P=0. 025); 2组患者治疗前肺功能指标无差别,治疗后,观察组患者肺功能指标优于对照组; 2组患者治疗前炎性反应因子水平无差别,治疗后,观察组IL-6、hs-CRP和TNF-α水平均低于对照组; 2组患者治疗前TGF-β1、Smad2、Smad7水平无差别,治疗后,观察组TGF-β1、Smad2、Smad7水平均低于对照组。结论:宣肺平喘方对哮喘有较好的治疗效果,可明显改善患者的TGF-β1、Smad2、Smad7表达水平和肺功能,具有良好的临床应用价值。
Objective: To observe the therapeutic effect and the influence of Smad7 on the levels of TGF-β1,Smad2 and protein in the treatment of asthma. Methods: Asthma patients treated in our hospital from March 2016 to March 2017 were selected as the research object,according to the treatment methods are divided into control group and observation group,the control group was given routine drug treatment,the observation group was given Xuanfei Pingchuan decoction treatment. The therapeutic effects of the two groups were observed,and the differences of lung function,inflammatory factors,TGF-beta 1,Smad 2 and Smad7 levels between the two groups before and after treatment were compared. Results: The effective rate of the observation group was 97. 50%,which was higher than that of the control group( χ2= 5. 000,P = 0. 025); There was no difference in lung function between the two groups before treatment. After treatment,the lung function index of the observation group was better than that of the control group; Two groups of patients had no difference between the levels of proinflammatory cytokines,after treatment,the observation group IL-6,hsCRP and TNF-alpha water were lower than those in control group; The levels of TGF-,beta 1,Smad2 and Smad7 in the two groups before treatment were not different. After treatment,the levels of TGF-,beta 1,Smad2 and Smad7 in the observation group were lower than those in the control group. Conclusion: The method of clearing lung and relieving asthma has better therapeutic effect on asthma. It can obviously improve the expression level of TGF-,beta 1,Smad2,Smad7 and lung function in patients with asthma,and has good clinical value.
Objective: To observe the therapeutic effect and the influence of Smad7 on the levels of TGF-β1,Smad2 and protein in the treatment of asthma. Methods: Asthma patients treated in our hospital from March 2016 to March 2017 were selected as the research object,according to the treatment methods are divided into control group and observation group,the control group was given routine drug treatment,the observation group was given Xuanfei Pingchuan decoction treatment. The therapeutic effects of the two groups were observed,and the differences of lung function,inflammatory factors,TGF-beta 1,Smad 2 and Smad7 levels between the two groups before and after treatment were compared. Results: The effective rate of the observation group was 97. 50%,which was higher than that of the control group( χ2= 5. 000,P = 0. 025); There was no difference in lung function between the two groups before treatment. After treatment,the lung function index of the observation group was better than that of the control group; Two groups of patients had no difference between the levels of proinflammatory cytokines,after treatment,the observation group IL-6,hsCRP and TNF-alpha water were lower than those in control group; The levels of TGF-,beta 1,Smad2 and Smad7 in the two groups before treatment were not different. After treatment,the levels of TGF-,beta 1,Smad2 and Smad7 in the observation group were lower than those in the control group. Conclusion: The method of clearing lung and relieving asthma has better therapeutic effect on asthma. It can obviously improve the expression level of TGF-,beta 1,Smad2,Smad7 and lung function in patients with asthma,and has good clinical value.
引文
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[7]郑贵浪,吴家兴.孟鲁司特治疗儿童支气管哮喘的临床疗效及肺功能和细胞因子变化研究[J].中国全科医学,2015,18(12):1387-1389,1395.
[8]徐哲,石平,曾茄,等.舒利迭气雾剂吸入及氟替卡松气雾剂吸入联合孟鲁司特口服治疗哮喘比较观察[J].山东医药,2017,57(11):80-83.
[9]张业清,苏克雷,庞中化,等.宣肺平喘方对哮喘小鼠气道炎性反应及Th17/Treg平衡的影响[J].中国实验方剂学杂志,2016,11(6):119-123.
[10]薛晓明,王洋,赵勤萍,等.宣肺平喘胶囊对慢性阻塞性肺疾病大鼠模型的影响[J].中国实验方剂学杂志,2017,37(2):120-125.
[11]王瑶瑶,杨莲芳,蒋丽芳.小儿哮喘炎性反应因子测定与免疫和气道功能的关系探讨[J].中国卫生检验杂志,2017,12(5):677-679.
[12]阮晓琳,周星星,彭来君,等.气阴双补中药对哮喘大鼠肺组织TGF-β1及Smad2/3表达的影响[J].中华中医药杂志,2017,10(3):1271-1275.
[13]王海亮,赵威,高志卿,等.益肾化瘀方含药血清对NRK52E细胞转分化过程中p-Smad2/3、Smad7表达的影响[J].中华中医药杂志,2015,15(12):4482-4485.
[14]方向明,王智星,邬锡琴.平喘宁对哮喘大鼠肺组织TGF-β1、Cyclin D1及p-ERK1/2mRNA表达的影响[J].中华中医药杂志,2016,31(1):296-299.
[15]冯佳,向阳,夏燕,等.益气养阴方对大鼠肺纤维化的干预作用及对Smad2、Smad7蛋白的影响[J].中国免疫学杂志,2015,31(3):334-338.
[16]佟雷,孙琳林,刘金丽,等.不同补肾阴方药对慢性哮喘气道重构的调节作用及其作用机制研究[J].中国中药杂志,2017,42(8):1585-1590.
[2]李冀,王艳丽,李志军,等.中医药治疗哮喘的研究进展[J].中医药信息,2014,31(1):114-117.
[3]张同文,郭敏.硫酸镁注射液联合孟鲁司特治疗支气管哮喘的效果及机制[J].山东医药,2015,55(25):60-62.
[4]张娴,赵雪琴.孟鲁司特钠联合布地奈德治疗儿童哮喘的临床研究[J].中国临床药理学杂志,2016,32(13):1169-1171.
[5]Turner MO,Johnston PR,Pizzichini E,et al. Anti-inflammatory effects of salmeterol compared with beclomethasone in eosinophilic mild exacerbations of asthma:a randomized,placebo controlled trial[J]. Can Respir J,1998,5(4):261-268.
[6]李献超.麻杏石甘汤治疗支气管哮喘急发期的临床观察[J].中药药理与临床,2015,35(1):292-293.
[7]郑贵浪,吴家兴.孟鲁司特治疗儿童支气管哮喘的临床疗效及肺功能和细胞因子变化研究[J].中国全科医学,2015,18(12):1387-1389,1395.
[8]徐哲,石平,曾茄,等.舒利迭气雾剂吸入及氟替卡松气雾剂吸入联合孟鲁司特口服治疗哮喘比较观察[J].山东医药,2017,57(11):80-83.
[9]张业清,苏克雷,庞中化,等.宣肺平喘方对哮喘小鼠气道炎性反应及Th17/Treg平衡的影响[J].中国实验方剂学杂志,2016,11(6):119-123.
[10]薛晓明,王洋,赵勤萍,等.宣肺平喘胶囊对慢性阻塞性肺疾病大鼠模型的影响[J].中国实验方剂学杂志,2017,37(2):120-125.
[11]王瑶瑶,杨莲芳,蒋丽芳.小儿哮喘炎性反应因子测定与免疫和气道功能的关系探讨[J].中国卫生检验杂志,2017,12(5):677-679.
[12]阮晓琳,周星星,彭来君,等.气阴双补中药对哮喘大鼠肺组织TGF-β1及Smad2/3表达的影响[J].中华中医药杂志,2017,10(3):1271-1275.
[13]王海亮,赵威,高志卿,等.益肾化瘀方含药血清对NRK52E细胞转分化过程中p-Smad2/3、Smad7表达的影响[J].中华中医药杂志,2015,15(12):4482-4485.
[14]方向明,王智星,邬锡琴.平喘宁对哮喘大鼠肺组织TGF-β1、Cyclin D1及p-ERK1/2mRNA表达的影响[J].中华中医药杂志,2016,31(1):296-299.
[15]冯佳,向阳,夏燕,等.益气养阴方对大鼠肺纤维化的干预作用及对Smad2、Smad7蛋白的影响[J].中国免疫学杂志,2015,31(3):334-338.
[16]佟雷,孙琳林,刘金丽,等.不同补肾阴方药对慢性哮喘气道重构的调节作用及其作用机制研究[J].中国中药杂志,2017,42(8):1585-1590.