蜣螂疏水壳聚糖的制备及其止血活性研究
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摘要
目的制备蜣螂疏水壳聚糖,研究其止血性能。方法以蜣螂壳聚糖为原料,通过与月桂酸酐酰基化反应,进行疏水改性与表征,同时采用离体凝集实验与小鼠肝创面实验研究其凝血性能。结果制备的蜣螂疏水壳聚糖疏水基取代度为(11.69±0.14)%,高于疏水市售壳聚糖;离体凝集实验表明其与血液混合后质量分数达6.0 mg/g时即可快速凝血(3.42±0.39)s,较蜣螂壳聚糖与市售疏水壳聚糖效果更优;小鼠肝创面实验显示蜣螂疏水壳聚糖的止血时间较蜣螂壳聚糖与疏水市售壳聚糖明显缩短。结论采用疏水改性可明显提高壳聚糖的凝血性能,且蜣螂疏水壳聚糖最为优良,有望成为一种有巨大潜力的新型止血材料。
Objective To prepare hydrophobic chitosan from Catharsius molossus and study its hemostatic effect. Methods The chitosan from C. molossus was used as material to carry out hydrophobic modification and characterization by acylation with lauric anhydride. At the same time, the hemostatic effect was studied by in vitro agglutination test and mouse liver wound experiment.Results The prepared hydrophobic chitosan had a hydrophobic group substitution degree of(11.69 ± 0.14)%, which was higher than that of commercially available hydrophobic chitosan. The in vitro agglutination experiment showed that it could coagulate in(3.42 ± 0.39) s when mixed with blood at a concentration of 6.0 mg/g, which was better than chitosan and hydrophobically modified commercial chitosan. The mouse liver wound experiment showed that chitosan from C. molossus could shorten hemostatic time significantly compared with chitosan and hydrophobically modified commercial chitosan. Conclusion Hydrophobically modified chitosan can significantly improve the coagulation performance of chitosan, and the hydrophobic chitosan from C. molossus is excellent, and it is expected to be a new hemostatic material with great potential.
Objective To prepare hydrophobic chitosan from Catharsius molossus and study its hemostatic effect. Methods The chitosan from C. molossus was used as material to carry out hydrophobic modification and characterization by acylation with lauric anhydride. At the same time, the hemostatic effect was studied by in vitro agglutination test and mouse liver wound experiment.Results The prepared hydrophobic chitosan had a hydrophobic group substitution degree of(11.69 ± 0.14)%, which was higher than that of commercially available hydrophobic chitosan. The in vitro agglutination experiment showed that it could coagulate in(3.42 ± 0.39) s when mixed with blood at a concentration of 6.0 mg/g, which was better than chitosan and hydrophobically modified commercial chitosan. The mouse liver wound experiment showed that chitosan from C. molossus could shorten hemostatic time significantly compared with chitosan and hydrophobically modified commercial chitosan. Conclusion Hydrophobically modified chitosan can significantly improve the coagulation performance of chitosan, and the hydrophobic chitosan from C. molossus is excellent, and it is expected to be a new hemostatic material with great potential.
引文
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[2]黄勇.壳聚糖碱性氨基酸衍生物的合成及抑菌活性研究[D].北京:中国科学院研究生院(海洋研究所),2016.
[3]Ming K,Chen X G,Ke X,et al.Antimicrobial properties of chitosan and mode of action:A state of the art review[J].Int J Food Microbiol,2010,144(1):51-63.
[4]赵瑞.壳聚糖改性及壳聚糖止血材料的止血作用和安全性研究[D].青岛:中国海洋大学,2015.
[5]何琴.疏水改性壳聚糖的制备、表征及止血评价[D].天津:天津工业大学,2013.
[6]石凉,汪涛,吴大洋.壳聚糖止血材料及最新研究进展[J].蚕业科学,2009,35(4):929-934.
[7]马家骅,谭承佳,邓霞,等.蜣螂药渣中壳聚糖的回收与初步表征[J].中药材,2012,35(7):129-1032.
[8]刘奇,药萌瑶,韩萍,等.壳聚糖改性方法研究进展[J].能源与环境,2015(2):12-13.
[9]Ma J,Chao X,Tan C.Preparation,physicochemical and pharmaceutical characterization of chitosan from Catharsius molossus residue[J].Int J Biol Macromol,2015,80:547-556.
[10]中国药典[S].四部.2015.
[11]赵颖,杨琰,卢实,等.壳聚糖脱乙酰度两种测定方法的比较[J].武汉轻工大学学报,2009,28(2):15-17.
[12]宣寒.一点法测定壳聚糖的特性黏度[J].安徽建筑大学学报,2008,16(3):82-84.
[13]温华强,马家骅,王静,等.疏水壳聚糖的制备及其凝血性能[J].精细化工,2018,35(8):1370-1375.
[14]汤朝晖,周露婷,谢志芳,等.利用分叶顺次肝切除术建立小鼠肝脏大部切除后再生模型[J].第二军医大学学报,2009,30(5):524-526.
[15]李静.壳聚糖的改性及其止血功能研究[D].长春:长春理工大学,2015.
[16]王艺旋,杨志伟,单天娇.壳聚糖的改性研究进展[J].合成技术及应用,2016,31(3):16-20.
[17]张宜,喻晶,曹利利,等.基于止血药研究的兔止血模型概述[J].华南国防医学杂志,2015,29(7):560-562.
[18]苗明三,尹俊涛,缪君娴,等.中药外用药理实验存在问题及解决思路[J].时珍国医国药,2008,19(12):2945-2946.
[19]陈奇.中药药理研究方法学[M].第2版.北京:人民卫生出版社,2006.