甘薯汁对果蝇寿命及抗氧化能力的影响
|
摘要
为探讨甘薯汁抗氧化及防衰老的功效,选取果蝇为动物模型,在培养基中分别添加低、中、高剂量(分别为0.10、0.15、0.20 mg/mL)甘薯汁饲喂8 h内羽化未交配的雌、雄果蝇,通过果蝇生存实验及测定不同饲养时间果蝇体内的超氧化物歧化酶(superoxide dismutase,SOD)活力、总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malondialdehyde,MDA)含量,评价甘薯汁对果蝇寿命和抗氧化酶活性的影响。结果表明:与对照组相比,不同剂量甘薯汁组均能显著延长雌、雄果蝇的平均寿命(P<0.05或P<0.01),且呈剂量依赖性。果蝇SOD活力、T-AOC在低、中、高剂量组中均显著增加(P<0.05或P<0.01),中、高剂量组果蝇体内MDA含量显著降低(P<0.05或P<0.01)。其中,与对照组相比,高剂量组可使雌、雄果蝇平均寿命分别延长17.93%和16.00%,SOD活力分别提高50.51%、58.61%,T-AOC分别提高79.73%、62.67%,MDA含量分别下降35.59%、37.14%。结论:甘薯汁可以提高果蝇体内的抗氧化能力,具有一定的延缓衰老作用。
In order to investigate the antioxidant and anti-aging effect of sweet potato juice, Drosophila melanogaster was chosen as an animal model. Male and female Drosophila melanogaster with eclosion and without mating within 8 h were fed on a diet supplemented with low, moderate or high doses(0.10, 0.15, and 0.20 mg/mL) of sweet potato juice. The survival rate of D. melanogaster was investigated, and the activity of superoxide dismutase(SOD), total antioxidant capacity(T-AOC) and malondialdehyde(MDA) content were measured in its homogenate. The results showed that compared with the control group, average life span and maximum life span in all dose groups were significantly increased(P < 0.05 or P < 0.01) in a dose-dependent manner. SOD activity and T-AOC were significantly increased in all dose groups(P < 0.05 or P < 0.01), whereas MDA content was significantly decreased in the middle-and high-dose groups(P < 0.05 or P < 0.01). Compared with the control group, average life span in the high-dose group was extended by 17.93% and 16.00% for female and male flies, the activity of SOD was increased by 50.51% and 58.61%, T-AOC activity was increased by 79.73% and 62.67%,while the content of MDA was decreased by 35.59% and 37.14%, respectively. In conclusion, sweet potato juice can prolong life span and delay aging in D. melanegaster.
In order to investigate the antioxidant and anti-aging effect of sweet potato juice, Drosophila melanogaster was chosen as an animal model. Male and female Drosophila melanogaster with eclosion and without mating within 8 h were fed on a diet supplemented with low, moderate or high doses(0.10, 0.15, and 0.20 mg/mL) of sweet potato juice. The survival rate of D. melanogaster was investigated, and the activity of superoxide dismutase(SOD), total antioxidant capacity(T-AOC) and malondialdehyde(MDA) content were measured in its homogenate. The results showed that compared with the control group, average life span and maximum life span in all dose groups were significantly increased(P < 0.05 or P < 0.01) in a dose-dependent manner. SOD activity and T-AOC were significantly increased in all dose groups(P < 0.05 or P < 0.01), whereas MDA content was significantly decreased in the middle-and high-dose groups(P < 0.05 or P < 0.01). Compared with the control group, average life span in the high-dose group was extended by 17.93% and 16.00% for female and male flies, the activity of SOD was increased by 50.51% and 58.61%, T-AOC activity was increased by 79.73% and 62.67%,while the content of MDA was decreased by 35.59% and 37.14%, respectively. In conclusion, sweet potato juice can prolong life span and delay aging in D. melanegaster.
引文
[1]江阳,孙成均.甘薯的营养成分及其保健功效研究进展[J].中国农业科技导报, 2010, 12(4): 56-61. DOI:10.3969/j.issn.1008-0864.2010.04.11.
[2] EDGE A S B, SPIRO R G. Structure of the O-linked oligosaccharides from a major thyroid cell surface glycoprotein[J]. Archives of Biochemistry and Biophysics, 1997, 343(1): 73-80. DOI:10.1006/abbi.1997.0143.
[3]徐敏.人体自由基与衰老的关系[J].临床和实验医学杂志,2006,5(3): 289-290. DOI:10.3969/j.issn.1671-4695.2006.03.077.
[4]李津.紫甘薯提取物对果蝇抗衰老作用的机理[D].天津:天津科技大学,2012:22-23.
[5]陈永强,刘金娟,曹成亮,等.紫甘薯汁抗肿瘤活性及诱导人肝癌细胞HepG2凋亡机制研究[J].食品科学, 2013, 34(1): 263-267.
[6] HWANG Y P, CHOI J H, CHOI J M, et al. Protective mechanisms of anthocyanins from purple sweet potato against tert-butyl hydroperoxide-induced hepatotoxicity[J]. Food and Chemical Toxicology, 2011, 49(9): 2081-2089. DOI:10.1016/j.fct.2011.05.021.
[7]雷鸣,卢晓黎,毛莉娟.我国甘薯食品开发现状及发展趋势[J].粮油加工与食品机械, 2002(11): 12-14.
[8]VAN AN L,FRANKOW-LINDBERG B E,LINDBERG J E.Effect of harvesting interval and defoliation yield and chemical composition of leaves, stems and tubers of sweetpotato (Ipomoea batatas L.(Iam.) ) plant parts[J]. Field Crops Research, 2003, 82(1): 49-58. DOI:10.1016/S0378-4290(03)00018-2.
[9]孙敏杰.甘薯蛋白的营养特性研究[D].北京:中国农业科学院,2012:3-4.
[10]綦菁华,王有年,于同泉,等.不同品种桃的酚类活性成分及其抗氧化功能研究[J].食品与发酵工业, 2006, 32(1): 103-106. DOI:10.3221/j.issn:0253-990X.2006.01.025.
[11]潘丽军,孙潇雅,姜绍通.紫薯复合饮料的质量指标、营养成分及抗氧化性分析[J].食品科学, 2013, 34(20): 165-169. DOI:10.7506/spkx1002-6630-201320034.
[12]RE R,PELLEGRINI N,PROTEGGENTE A,et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Radical Biology and Medicine, 1999, 26(9/10): 1231-1237.DOI:10.1016/S0891-5849(98)00315-3.
[13] RUMBAOA R G O, COMAGO D F, GERONIMO I M. Phenolic content and antioxidant capacity of Philippine sweet potato (Ipomoea batatas) varieties[J]. Food Chemistry, 2009, 113(4): 1133-1138. DOI:10.1016/j.foodchem.2008.08.088.
[14]黄玲艳.桂花提取物的抗氧化及延缓衰老作用研究[D].扬州:扬州大学,2017:35-36.
[15]孙振欧,王晓彬,王红,等.黑豆皮提取物升高内源抗氧化酶延长果蝇寿命[J].食品研究与开发, 2016, 37(18): 11-14; 48. DOI:10.3969/j.issn.1005-6521.2016.18.003.
[16]段丹丹,高丽,王珂欣,等.黄芩素通过抗氧化应激延长果蝇寿命的机制[J].药学学报, 2016, 51(9): 1401-1406. DOI:10.16438/j.0513-4870.2016-0248.
[17]王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009, 23(3): 40-42. DOI:10.3969/j.issn.1008-1267.2009.03.016.
[18]王娟,任莉萍,屈长青,等.香椿子对果蝇的寿命及其抗氧化相关指标的影响作用[J].天然产物研究与开发, 2015, 27(11): 1858-1861; 1865.DOI:10.16333/j.1001-6880.2015.11.004.
[19]丘婷,吴思,陈朋,等.六味地黄生物制剂多糖对果蝇抗氧化作用的影响[J].中国实验方剂学杂志, 2012, 18(22): 215-218. DOI:10.13422/j.cnki.syfjx.2012.22.037.
[20]黄杰,王华丽,马娜,等.迷迭香提取物抗氧化作用及其对果蝇寿命的影响[J].中国食品学报, 2016, 16(5): 19-24. DOI:10.16429/j.1009-7848.2016.05.003.
[21] CUTLER R G, SEMSEI I. Development, cancer and aging: possible common mechanisms of action and regulation[J]. The Journal of Gerontology, 1989, 44(6): 25-34. DOI:10.1093/geronj/44.6.25.
[22] CUTLER R G. Antioxidants and aging[J]. The American Society for Clinical Nutrition, 1991, 53(1): 3735-3795.
[23] CUTLER R G, KELLY J, STORIE K, et al. Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimer’s disease[J]. Proceedings of the National Academy of Sciences, 2004, 101(7): 2070-2075.
[24] WANG Haifeng, ZHONG Xiuhui, SHI Wanyu, et al. Study of malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in chickens infected with avian infectious bronchitis virus[J]. African Journal of Biotechnology, 2011, 10(45): 9213-9217.
[25] TOLMASOFF J M, ONO T, CUTLER R G. Superoxide dismutase: correlation with life-span and specific metabolic rate in primate species[J]. Proceedings of the National Academy of Sciences, 1980, 77(5): 2777-2781. DOI:10.1073/pnas.77.5.2777.
[26]张欣文,徐思红,厉曙光.果蝇体内SOD和MDA随增龄变化及其与寿命的关系[J].中国公共卫生, 2000, 16(3): 222-223. DOI:10.3321/j.issn:1001-0580.2000.03.014.
[27]梁亦龙,王允,王孔民.归脾汤对果蝇寿命的影响及抗氧化研究[J].广东化工, 2017, 44(20): 215; 218.
[28]赵江,陈纯,刘素稳,等.紫薯提取物对雌性果蝇寿命的影响及其作用机制[J].中国食品添加剂, 2014(7): 147-152. DOI:10.3969/j.issn.1006-2513.2014.07.018.
[29]李尽哲,黄雅琴,庞瑞华,等.虫草素对果蝇生长发育和寿命的影响[J].食用菌学报, 2016, 23(1): 67-70. DOI:10.16488/j.cnki.1005-9873.2016.01.014.
[30]李飞,隋新.长白山虫草属蛹虫草多糖的抗氧化活性及其对果蝇寿命的影响[J].中国食品添加剂, 2016(4): 69-73. DOI:10.3969/j.issn.1006-2513.2016.04.003.
[31]孙晨倩,王正齐,姚美,等.花椒叶的化学组成、叶提取物体外抗氧化活性及其对黑腹果蝇抗氧化酶活性的影响[J].植物资源与环境学报, 2015, 24(4): 38-44. DOI:10.3969/j.issn.1674-7895.2015.04.05.
[32]邵婵.酸枣提取物对果蝇抗衰老机理的研究[D].天津:天津科技大学,2012:22-23.
[33]陈纯.紫薯提取物对果蝇和线虫抗氧化作用的研究[D].天津:天津科技大学,2016:29-30.
[34]张明,何超,邵颖,等.蛹虫草多糖对果蝇寿命及抗氧化活性的影响[J].食品科技, 2016, 41(11): 179-183. DOI:10.13684/j.cnki.spkj.2016.11.041.
[35] HELFAND S L, ROGINA B. Genetics of aging in the fruit fly, Drosophila melanogaster[J]. Annual Review of Genetics, 2003, 37(1): 329-348.DOI:10.1146/annurev.genet.37.040103.095211.
[36] SAUNDERS R D C, BOUBRIAK I, CLANCY D J, et al. Identification and characterization of a Drosophila ortholog of WRN exonuclease that is required to maintain genome integrity[J]. Aging Cell, 2008, 7(3): 418-425. DOI:10.1111/j.1474-9726.2008.00388.x.
[37]刘梅,郑美,史挺.壳聚糖对果蝇生长发育及抗氧化能力的影响[J].食品工业科技, 2015, 36(24): 349-351; 355. DOI:10.13386/j.issn.1002-0306.2015.24.068.
[38]欧海龙,张庆海.姜黄素对果蝇抗氧化能力和衰老的影响[J].云南农业大学学报(自然科学版), 2015, 30(4): 594-598. DOI:10.16211/j.issn.1004-390X(n).2015.04.017.
[39]黄杰,刘素稳,马娜,等.紫薯提取物抗氧化及延长寿命作用的研究[J].营养学报, 2015, 37(5): 283-287. DOI:10.13325/j.cnki.acta.nutr.sin.2015.05.023.
[2] EDGE A S B, SPIRO R G. Structure of the O-linked oligosaccharides from a major thyroid cell surface glycoprotein[J]. Archives of Biochemistry and Biophysics, 1997, 343(1): 73-80. DOI:10.1006/abbi.1997.0143.
[3]徐敏.人体自由基与衰老的关系[J].临床和实验医学杂志,2006,5(3): 289-290. DOI:10.3969/j.issn.1671-4695.2006.03.077.
[4]李津.紫甘薯提取物对果蝇抗衰老作用的机理[D].天津:天津科技大学,2012:22-23.
[5]陈永强,刘金娟,曹成亮,等.紫甘薯汁抗肿瘤活性及诱导人肝癌细胞HepG2凋亡机制研究[J].食品科学, 2013, 34(1): 263-267.
[6] HWANG Y P, CHOI J H, CHOI J M, et al. Protective mechanisms of anthocyanins from purple sweet potato against tert-butyl hydroperoxide-induced hepatotoxicity[J]. Food and Chemical Toxicology, 2011, 49(9): 2081-2089. DOI:10.1016/j.fct.2011.05.021.
[7]雷鸣,卢晓黎,毛莉娟.我国甘薯食品开发现状及发展趋势[J].粮油加工与食品机械, 2002(11): 12-14.
[8]VAN AN L,FRANKOW-LINDBERG B E,LINDBERG J E.Effect of harvesting interval and defoliation yield and chemical composition of leaves, stems and tubers of sweetpotato (Ipomoea batatas L.(Iam.) ) plant parts[J]. Field Crops Research, 2003, 82(1): 49-58. DOI:10.1016/S0378-4290(03)00018-2.
[9]孙敏杰.甘薯蛋白的营养特性研究[D].北京:中国农业科学院,2012:3-4.
[10]綦菁华,王有年,于同泉,等.不同品种桃的酚类活性成分及其抗氧化功能研究[J].食品与发酵工业, 2006, 32(1): 103-106. DOI:10.3221/j.issn:0253-990X.2006.01.025.
[11]潘丽军,孙潇雅,姜绍通.紫薯复合饮料的质量指标、营养成分及抗氧化性分析[J].食品科学, 2013, 34(20): 165-169. DOI:10.7506/spkx1002-6630-201320034.
[12]RE R,PELLEGRINI N,PROTEGGENTE A,et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Radical Biology and Medicine, 1999, 26(9/10): 1231-1237.DOI:10.1016/S0891-5849(98)00315-3.
[13] RUMBAOA R G O, COMAGO D F, GERONIMO I M. Phenolic content and antioxidant capacity of Philippine sweet potato (Ipomoea batatas) varieties[J]. Food Chemistry, 2009, 113(4): 1133-1138. DOI:10.1016/j.foodchem.2008.08.088.
[14]黄玲艳.桂花提取物的抗氧化及延缓衰老作用研究[D].扬州:扬州大学,2017:35-36.
[15]孙振欧,王晓彬,王红,等.黑豆皮提取物升高内源抗氧化酶延长果蝇寿命[J].食品研究与开发, 2016, 37(18): 11-14; 48. DOI:10.3969/j.issn.1005-6521.2016.18.003.
[16]段丹丹,高丽,王珂欣,等.黄芩素通过抗氧化应激延长果蝇寿命的机制[J].药学学报, 2016, 51(9): 1401-1406. DOI:10.16438/j.0513-4870.2016-0248.
[17]王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009, 23(3): 40-42. DOI:10.3969/j.issn.1008-1267.2009.03.016.
[18]王娟,任莉萍,屈长青,等.香椿子对果蝇的寿命及其抗氧化相关指标的影响作用[J].天然产物研究与开发, 2015, 27(11): 1858-1861; 1865.DOI:10.16333/j.1001-6880.2015.11.004.
[19]丘婷,吴思,陈朋,等.六味地黄生物制剂多糖对果蝇抗氧化作用的影响[J].中国实验方剂学杂志, 2012, 18(22): 215-218. DOI:10.13422/j.cnki.syfjx.2012.22.037.
[20]黄杰,王华丽,马娜,等.迷迭香提取物抗氧化作用及其对果蝇寿命的影响[J].中国食品学报, 2016, 16(5): 19-24. DOI:10.16429/j.1009-7848.2016.05.003.
[21] CUTLER R G, SEMSEI I. Development, cancer and aging: possible common mechanisms of action and regulation[J]. The Journal of Gerontology, 1989, 44(6): 25-34. DOI:10.1093/geronj/44.6.25.
[22] CUTLER R G. Antioxidants and aging[J]. The American Society for Clinical Nutrition, 1991, 53(1): 3735-3795.
[23] CUTLER R G, KELLY J, STORIE K, et al. Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimer’s disease[J]. Proceedings of the National Academy of Sciences, 2004, 101(7): 2070-2075.
[24] WANG Haifeng, ZHONG Xiuhui, SHI Wanyu, et al. Study of malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in chickens infected with avian infectious bronchitis virus[J]. African Journal of Biotechnology, 2011, 10(45): 9213-9217.
[25] TOLMASOFF J M, ONO T, CUTLER R G. Superoxide dismutase: correlation with life-span and specific metabolic rate in primate species[J]. Proceedings of the National Academy of Sciences, 1980, 77(5): 2777-2781. DOI:10.1073/pnas.77.5.2777.
[26]张欣文,徐思红,厉曙光.果蝇体内SOD和MDA随增龄变化及其与寿命的关系[J].中国公共卫生, 2000, 16(3): 222-223. DOI:10.3321/j.issn:1001-0580.2000.03.014.
[27]梁亦龙,王允,王孔民.归脾汤对果蝇寿命的影响及抗氧化研究[J].广东化工, 2017, 44(20): 215; 218.
[28]赵江,陈纯,刘素稳,等.紫薯提取物对雌性果蝇寿命的影响及其作用机制[J].中国食品添加剂, 2014(7): 147-152. DOI:10.3969/j.issn.1006-2513.2014.07.018.
[29]李尽哲,黄雅琴,庞瑞华,等.虫草素对果蝇生长发育和寿命的影响[J].食用菌学报, 2016, 23(1): 67-70. DOI:10.16488/j.cnki.1005-9873.2016.01.014.
[30]李飞,隋新.长白山虫草属蛹虫草多糖的抗氧化活性及其对果蝇寿命的影响[J].中国食品添加剂, 2016(4): 69-73. DOI:10.3969/j.issn.1006-2513.2016.04.003.
[31]孙晨倩,王正齐,姚美,等.花椒叶的化学组成、叶提取物体外抗氧化活性及其对黑腹果蝇抗氧化酶活性的影响[J].植物资源与环境学报, 2015, 24(4): 38-44. DOI:10.3969/j.issn.1674-7895.2015.04.05.
[32]邵婵.酸枣提取物对果蝇抗衰老机理的研究[D].天津:天津科技大学,2012:22-23.
[33]陈纯.紫薯提取物对果蝇和线虫抗氧化作用的研究[D].天津:天津科技大学,2016:29-30.
[34]张明,何超,邵颖,等.蛹虫草多糖对果蝇寿命及抗氧化活性的影响[J].食品科技, 2016, 41(11): 179-183. DOI:10.13684/j.cnki.spkj.2016.11.041.
[35] HELFAND S L, ROGINA B. Genetics of aging in the fruit fly, Drosophila melanogaster[J]. Annual Review of Genetics, 2003, 37(1): 329-348.DOI:10.1146/annurev.genet.37.040103.095211.
[36] SAUNDERS R D C, BOUBRIAK I, CLANCY D J, et al. Identification and characterization of a Drosophila ortholog of WRN exonuclease that is required to maintain genome integrity[J]. Aging Cell, 2008, 7(3): 418-425. DOI:10.1111/j.1474-9726.2008.00388.x.
[37]刘梅,郑美,史挺.壳聚糖对果蝇生长发育及抗氧化能力的影响[J].食品工业科技, 2015, 36(24): 349-351; 355. DOI:10.13386/j.issn.1002-0306.2015.24.068.
[38]欧海龙,张庆海.姜黄素对果蝇抗氧化能力和衰老的影响[J].云南农业大学学报(自然科学版), 2015, 30(4): 594-598. DOI:10.16211/j.issn.1004-390X(n).2015.04.017.
[39]黄杰,刘素稳,马娜,等.紫薯提取物抗氧化及延长寿命作用的研究[J].营养学报, 2015, 37(5): 283-287. DOI:10.13325/j.cnki.acta.nutr.sin.2015.05.023.