玉米RPS14 cDNA的克隆及序列分析
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摘要
为了研究玉米RPS14基因的序列信息,以玉米‘南-22号’为实验材料,根据已报道的部分核糖体蛋白S14亚基基因的相关信息,利用RT-PCR克隆出玉米RPS14基因的cDNA序列,并对序列进行了分析。结果表明,玉米RPS14基因的开放性阅读框(ORF)长453 bp,编码150个氨基酸,GenBank登陆号为JX232265。蛋白质的相对分子量约为16.16 kD,等电点pI为11.35。该蛋白含有7个不同功能位点。该基因的cDNA序列及其编码的氨基酸序列,与已报道的部分植物RPS14基因有很高的同源性。本研究为进一步研究RPS14基因的功能提供了基础。
In order to investigate the sequence information of maize RPS14 gene in the cDNA sequence of maize RPS14 gene was cloned by RT-PCR according to Zea mays 'Nan-22' as experimental material, and the sequence was also analyzed. The results showed that the open reading frame(ORF) of RPS14 was 453 bp in length, which encode 150 amino acid. GenBank accession number was JX232265. Primary structure analysis revealed that the molecular weight of putative RPS14 protein is approximately 16.16 kD, with a theoretical pI 11.35. And this protein contains seven different types of functional sites. The cDNA sequence and encoded amino acid sequence of this gene were highly similar to the previous reported sequences derived from other plants. This study provides a basis for further study of the function of the RPS14 gene.
In order to investigate the sequence information of maize RPS14 gene in the cDNA sequence of maize RPS14 gene was cloned by RT-PCR according to Zea mays 'Nan-22' as experimental material, and the sequence was also analyzed. The results showed that the open reading frame(ORF) of RPS14 was 453 bp in length, which encode 150 amino acid. GenBank accession number was JX232265. Primary structure analysis revealed that the molecular weight of putative RPS14 protein is approximately 16.16 kD, with a theoretical pI 11.35. And this protein contains seven different types of functional sites. The cDNA sequence and encoded amino acid sequence of this gene were highly similar to the previous reported sequences derived from other plants. This study provides a basis for further study of the function of the RPS14 gene.
引文
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Daftuar L.,Zhu Y.,Jacq X.,and Prives C.,2013,Ribosomal proteins RPL37,RPS15 and RPS20 regulate the Mdm2-p53-MdmX network,PLoS One,8(7):e68667
Fan Y.R.,Liu Y.,and Zhang F.Y.,2013,Cloning,expression and function preliminary research of RPS14 in Arabidopsis thaliana,Shengwu Jishu Tongbao(Biotechnology Bulletin),32(1):73-77(范艳荣,刘莹,张飞云,2013,拟南芥RPS14基因的克隆表达及功能初步研究,生物技术通报,32(1):73-77)
Hao Y.Z.,Hou W.R.,Du Y.J.,and Zhang T.,2009,cDNA cloning and sequences analysis of LSM3 from giant panda,Sichuan Dongwu(Sichuan Journal of Zoology),28(2):181-183,188(郝彦哲,侯万儒,杜玉杰,张田,2009,大熊猫LSM3c DNA序列的克隆及序列分析,四川动物,28(2):181-183,188)
Kim Y.,Kim H.D.,and Kim J.,2013,Cytoplasmic ribosomal protein S3(rpS3)plays a pivotal role in mitochondrial DNAdamage surveillance,Biochim.Biophys.Acta,1833(12):2943-2952
Liu S.,and Zhao D.G.,2015,Cloningand expression analysisof RksAGL gene in seedless chestnut rose(Rosa kweichonensis var.sterilis),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(3):579-586(刘松,赵德刚,2015,无籽刺梨(Rosa kweichonensis var.sterilis)RksAGL基因克隆及表达分析,基因组学与应用生物学,34(3):579-586)
Meng G.,Zhai R.,and Chen B.,2008,Identification and characterization of human adenylate kinase 6 homologues from Drosophila melanogaster,Biochemistry(Moscow),73(1):38-43
Sen N.,Paul B.D.,Gadalla M.M.,Mustafa A.K.,Sen T.,Xu R.,Kim S.,and Snyder S.H.,2012,Hydrogen sulfide-linked sulfhydration of NF-kappaB mediates its antiapoptotic actions,Mol.Cell,45(1):13-24
Seong K.M.,Jung S.O.,Kim H.D.,Kim H.J.,Jung Y.J.,Choi S.Y.,and Kim J.,2012,Yeast ribosomal protein S3 possesses a beta-lyase activity on damaged DNA,FEBS Lett.,586(4):356-361
Wang T.,Hou Y.L.,Ding X.,Sun B.,Su X.L.,Li J.,and Hou W.R.,2015,CDNA cloning and sequence analysis of ribosomal protein S16 gene from Sichuan black bear(Ursus thibetanus mupinensis),Xihua Shifan Daxue Xuebao(Journal of China West Normal University),33(4):350-355(王婷,侯怡铃,丁祥,孙冰,苏秀兰,李俊,侯万儒,2015,四川黑熊RPS16亚基基因的克隆与序列分析,西华师范大学学报33(4):350-355)
Wier E.M.,Neighoff J.,Sun X.,Fu K.,and Wan F.,2012,Identification of an N-terminal truncation of the NF-kappaB p65subunit that specifically modulates ribosomal protein S3-dependent NF-kappaB gene expression,J.Biol.Chem.,287(51):43019-43029
Yu Z.G.,and Wang H.T.,2008,Analysis of corn mechanization harvest in our country,Nongji Shiyong Yu Weixiu(Using and Repairing of Agricultural Machinery),(6):82-83(于志刚,王洪涛,2008,我国玉米收获机械化分析,农机使用与维修,(6):82-83)
Yuan F.,Wang S.B.,and Wang Y.L.,2017,Ribosomal protein family in tumorigenesis and development,Zhongliu Daixie Yu Yingyang Dianzizazhi(Electron Journal of Metabolism Nutrition Cancer),4(1):109-113(袁飞,王树斌,王月丽,2017,核糖体蛋白家族在肿瘤发生,发展中作用的研究进展,肿瘤代谢与营养电子杂志,4(1):109-113)
Zhao J.,Peng Z.S.,Yang Z.J.,Wei S.H.,and Peng L.J.,2011,Clong of the Pinellia plant agglutinin gene and sequences analysis,Xihua Shifan Daxue Xuebao(Journal of China West Normal University),32(1):7-11,41(赵静,彭正松,杨在君,魏淑红,彭丽娟,2011,半夏属植物凝集素基因片段克隆与序列分析,西华师范大学学报,32(1):7-11,41)
Daftuar L.,Zhu Y.,Jacq X.,and Prives C.,2013,Ribosomal proteins RPL37,RPS15 and RPS20 regulate the Mdm2-p53-MdmX network,PLoS One,8(7):e68667
Fan Y.R.,Liu Y.,and Zhang F.Y.,2013,Cloning,expression and function preliminary research of RPS14 in Arabidopsis thaliana,Shengwu Jishu Tongbao(Biotechnology Bulletin),32(1):73-77(范艳荣,刘莹,张飞云,2013,拟南芥RPS14基因的克隆表达及功能初步研究,生物技术通报,32(1):73-77)
Hao Y.Z.,Hou W.R.,Du Y.J.,and Zhang T.,2009,cDNA cloning and sequences analysis of LSM3 from giant panda,Sichuan Dongwu(Sichuan Journal of Zoology),28(2):181-183,188(郝彦哲,侯万儒,杜玉杰,张田,2009,大熊猫LSM3c DNA序列的克隆及序列分析,四川动物,28(2):181-183,188)
Kim Y.,Kim H.D.,and Kim J.,2013,Cytoplasmic ribosomal protein S3(rpS3)plays a pivotal role in mitochondrial DNAdamage surveillance,Biochim.Biophys.Acta,1833(12):2943-2952
Liu S.,and Zhao D.G.,2015,Cloningand expression analysisof RksAGL gene in seedless chestnut rose(Rosa kweichonensis var.sterilis),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(3):579-586(刘松,赵德刚,2015,无籽刺梨(Rosa kweichonensis var.sterilis)RksAGL基因克隆及表达分析,基因组学与应用生物学,34(3):579-586)
Meng G.,Zhai R.,and Chen B.,2008,Identification and characterization of human adenylate kinase 6 homologues from Drosophila melanogaster,Biochemistry(Moscow),73(1):38-43
Sen N.,Paul B.D.,Gadalla M.M.,Mustafa A.K.,Sen T.,Xu R.,Kim S.,and Snyder S.H.,2012,Hydrogen sulfide-linked sulfhydration of NF-kappaB mediates its antiapoptotic actions,Mol.Cell,45(1):13-24
Seong K.M.,Jung S.O.,Kim H.D.,Kim H.J.,Jung Y.J.,Choi S.Y.,and Kim J.,2012,Yeast ribosomal protein S3 possesses a beta-lyase activity on damaged DNA,FEBS Lett.,586(4):356-361
Wang T.,Hou Y.L.,Ding X.,Sun B.,Su X.L.,Li J.,and Hou W.R.,2015,CDNA cloning and sequence analysis of ribosomal protein S16 gene from Sichuan black bear(Ursus thibetanus mupinensis),Xihua Shifan Daxue Xuebao(Journal of China West Normal University),33(4):350-355(王婷,侯怡铃,丁祥,孙冰,苏秀兰,李俊,侯万儒,2015,四川黑熊RPS16亚基基因的克隆与序列分析,西华师范大学学报33(4):350-355)
Wier E.M.,Neighoff J.,Sun X.,Fu K.,and Wan F.,2012,Identification of an N-terminal truncation of the NF-kappaB p65subunit that specifically modulates ribosomal protein S3-dependent NF-kappaB gene expression,J.Biol.Chem.,287(51):43019-43029
Yu Z.G.,and Wang H.T.,2008,Analysis of corn mechanization harvest in our country,Nongji Shiyong Yu Weixiu(Using and Repairing of Agricultural Machinery),(6):82-83(于志刚,王洪涛,2008,我国玉米收获机械化分析,农机使用与维修,(6):82-83)
Yuan F.,Wang S.B.,and Wang Y.L.,2017,Ribosomal protein family in tumorigenesis and development,Zhongliu Daixie Yu Yingyang Dianzizazhi(Electron Journal of Metabolism Nutrition Cancer),4(1):109-113(袁飞,王树斌,王月丽,2017,核糖体蛋白家族在肿瘤发生,发展中作用的研究进展,肿瘤代谢与营养电子杂志,4(1):109-113)
Zhao J.,Peng Z.S.,Yang Z.J.,Wei S.H.,and Peng L.J.,2011,Clong of the Pinellia plant agglutinin gene and sequences analysis,Xihua Shifan Daxue Xuebao(Journal of China West Normal University),32(1):7-11,41(赵静,彭正松,杨在君,魏淑红,彭丽娟,2011,半夏属植物凝集素基因片段克隆与序列分析,西华师范大学学报,32(1):7-11,41)