高通量测序技术检测鸽群中病毒感染状况
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摘要
为了解我国鸽子病毒感染状况,采用高通量测序方法,对华东地区肉鸽携带的病原进行了检测,共检测到5种主要感染病原,包括禽轮状病毒、鸽圆环病毒、鸽星状病毒、鸽冠状病毒和鸽微RNA病毒B。除鸽圆环病毒外,其余4种病毒均在我国鸽群中首次被发现。该检测结果对我国的鸽群疫病监测和防控具有重要意义,同时证实高通量技术能够检测出动物群体中的主要感染病原,是动物疫病检测的新思路,有利于全面掌握动物病原感染状况,及时预警、处置和防控动物疫病的发生。
In order to recognize the virus infection situation in pigeons in China,the pathogens carried by pigeons in Eastern China were detected by high-throughput sequencing technology. The results showed five kinds of infectious viruses were detected,including avian rotavirus,pigeon circovirus,pigeon astrovirus,pigeon coronavirus and pigeon piconavirus B. Except pigeon circovirus,the other four kinds of viruses were all discovered in pigeons for the first time in China. The results of this study were significant to conduct pigeon disease surveillance,prevention and control. At the same time,it was confirmed that the high-throughput sequencing technique could detect main pathogens in animals,which would provide a new idea for animal disease detection. Also,the technique was helpful to master an overall situation of pathogen infection,and it was helpful for early warning,disposal and prevention of animal diseases.
In order to recognize the virus infection situation in pigeons in China,the pathogens carried by pigeons in Eastern China were detected by high-throughput sequencing technology. The results showed five kinds of infectious viruses were detected,including avian rotavirus,pigeon circovirus,pigeon astrovirus,pigeon coronavirus and pigeon piconavirus B. Except pigeon circovirus,the other four kinds of viruses were all discovered in pigeons for the first time in China. The results of this study were significant to conduct pigeon disease surveillance,prevention and control. At the same time,it was confirmed that the high-throughput sequencing technique could detect main pathogens in animals,which would provide a new idea for animal disease detection. Also,the technique was helpful to master an overall situation of pathogen infection,and it was helpful for early warning,disposal and prevention of animal diseases.
引文
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[2]PHAN T G,VO N P,BOROS A,et al.The viruses of wild pigeon droppings[J].Plos one,2013,8(9):e72787.
[3]QIU Y,CHEN J M,WANG T,et al.Detection of viromes of RNA viruses using the next generation sequencing libraries prepared by three methods[J].Virus Research,2017,237:22-26.
[4]MCGINNIS S,MADDEN T L.BLAST:at the core of a powerful and diverse set of sequence analysis tools[J].Nucleic acids research,2004,32:W20-25.
[5]HUSON D H,AUCH A F,QI J,et al.MEGAN analysis of metagenomic data[J].Genome research,2007,17(3):377-386.
[6]LIU T,XIE Z,WANG G,et al.Avian infl uenza virus with hemagglutinin-neuraminidase combination H3N6,isolated from a domestic pigeon in Guangxi,Southern China[J].Genome announc,2015,3(1):e00965.
[7]YAO Y,FU S,HE B,et al.Complete genome sequences of an h5n1 highly pathogenic avian influenza virus isolated from pigeon in China in 2012[J].Genome announc,2015,3(6):e01330.
[8]LIU H,WANG Z,SON C,et al.Characterization of pigeon-origin Newcastle disease virus isolated in China[J].Avian disease,2006,50(4):636-640.
[9]REN S,XIE X,WANG Y,et al.Molecular characterization of a Class I Newcastle disease virus strain isolated from a pigeon in China[J].Avian pathol,2016,45(4):408-417.
[10]WANG K C,ZHUANG Q Y,QIU Y,et al.Genome sequence characterization of pigeon circoviruses in China[J].Virus research,2017,233:1-7.
[11]ZHANG Z,DAI W,WANG S,et al.Epidemiology and genetic characteristics of pigeon circovirus(Pi CV)in eastern China[J].Archives of virology,2015,160(1):199-206.
[12]SHI S H,CHEN H M,CHEN Z,et al.Complete genome sequence of a duck hepatitis a virus 1 isolated from a pigeon in china[J].Genome announc,2013,1(4):e00451.
[13]ZHAI S L,CHEN S N,LIN T,et al.Emergence of reticuloendotheliosis virus in pigeons in Guangdong Province,Southern China[J].Archives of virology,2016,161(7):2007-2011.
[14]ZHANG Z,ZHUANG L,DAI W,et al.Complete genome sequence of a novel pigeon torque teno virus in china[J].Genome announc,2013,1(6):215-216.
[15]ROUSSAN D A,SHAHEEN I A,KHAWALDEH G Y,et al.Simultaneous detection of astrovirus,rotavirus,reovirus and adenovirus type I in broiler chicken flocks[J].Polish academy of sciences,2012,15(2):337-344.
[16]ZOU X,TANG G,ZHAO X,et al.Simultaneous virus identifi cation and characterization of severe unexplained pneumonia cases using a metagenomics sequencing technique[J].Science China life sciences,2017,60(3):279-286.
[17]AL-ATEEG F A.SARS:The emergence of a new epidemic[J].Saudi medical journal,2003,24(7):794-798.
[18]LI W,SHI Z,YU M,et al.Bats are natural reservoirs of SARS-like coronaviruses[J].Science,2005,310(5748):676-679.
[19]PARRY J.H7N9 virus is more transmissible and harder to detect than H5N1,say experts[J].British medical journal,2013,346:f2568.