网柄菌属9个种形态特征及其基因组大小分析
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摘要
为探究网柄细胞状黏菌的表观形态特征与其遗传信息之间的关系,选择细胞裂解液OTTO及荧光染料碘化丙啶,以酿酒酵母Saccharomyces cerevisiae为内标,采用流式细胞术对隶属于网柄菌属Dictyostelium的9个种进行基因组大小的测定,并将所获得的9种网柄细胞状黏菌基因组大小的数据分别与其孢堆果大小及孢子团直径的数值进行线性回归分析。结果表明,被测网柄细胞状黏菌基因组大小约为27.23～47.07 Mb,且其基因组大小与孢堆果大小和孢子团直径均呈正相关。
The aims of this study were to explore the correlations between apparent morphological characteristics and genetic information of the dictyostelid cellular slime molds species.Saccharomyces cerevisiae was used as an internal reference,and the genome size of 9 Dictyostelium species were estimated by Flow Cytometry in this study by adopting buffers OTTO and fluorochrome propidium iodide(PI) analyses.The correlations between the size of sorocarps and sori of these 9 Dictyostelium species with their genome sizes were analyzed by the linear regression method.The results showed that the genome sizes of those 9 species were ranged from 27.23 to 47.07 Mb,and the sizes of their sorocarps and sori diameter were both positively correlated with their genome sizes.
The aims of this study were to explore the correlations between apparent morphological characteristics and genetic information of the dictyostelid cellular slime molds species.Saccharomyces cerevisiae was used as an internal reference,and the genome size of 9 Dictyostelium species were estimated by Flow Cytometry in this study by adopting buffers OTTO and fluorochrome propidium iodide(PI) analyses.The correlations between the size of sorocarps and sori of these 9 Dictyostelium species with their genome sizes were analyzed by the linear regression method.The results showed that the genome sizes of those 9 species were ranged from 27.23 to 47.07 Mb,and the sizes of their sorocarps and sori diameter were both positively correlated with their genome sizes.
引文
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[15] 陈丙义,李金凤,霍恒志,万春雁,章镇,乔玉山,糜林.6种野生草莓基因组大小估算[J].果树学报,2015,32(1):51-56Chen B Y,Li J F,Huo H Z,Wan C Y,Zhang Z,Qiao Y S,Mi L.Estimation of genome size in six wild strawberry species[J].Journal of Fruit Science,2015,32(1):51-56 (in Chinese)
[16] 李秋实,徐江,朱英杰,孙超,宋经元,陈士林.基于流式细胞技术的灵芝基因组大小估测[J].菌物学报,2013,32(5):899-906Li Q S,Xu J,Zhu Y J,Sun C,Song J Y,Chen S L.Estimation of the genome size of Ganoderma lucidum based on a flow cytometric analysis[J].Mycosystema,2013,32(5):899-906 (in Chinese)
[17] 逄洪波,高秋,李玥莹,马纯艳,刘宏鑫,杨成瑶,吕瑞雪.利用流式细胞仪测定鬼针草基因组大小[J].基因组学与应用生物学,2016,35(7):1800-1804Pang H B,Gao Q,Li Y Y,Ma C Y,Liu H X,Yang C Y,Lv R X.Genome size estimation of Bidens bipinnata L by using flow cytometry[J].Genomics and Applied Biology,2016,35(7):1800-1804 (in Chinese)
[18] 王亚之,李秋实,陈士林,孙超,宋经元.基于流式细胞分析技术的茯苓基因组大小测定[J].世界科学技术:中医药现代化,2010,12(3):452-456Wang Y Z,Li Q S,Chen S L,Sun C,Song J Y.Flow cytometric analysis of nuclear DNA content in Poria cocos[J].World Science and Technology (Modernization of Traditional Chinese Medicine and Materia Medica),2010,12(3):452-456 (in Chinese)
[19] 李聪.酿酒酵母培养条件及发酵培养基的优化[J].中国农学通报,2014,30(9):302-306Li C.Optimization of culturing conditions and fermentation culture media of Saccharomy cescerevisiae[J].Chinese Agricultural Science Bulletin,2014,30(9):302-306 (in Chinese)
[20] Dole?el J,Greilhuber J,Suda J.Estimation of nuclear DNA content in plants using flow cytometry[J].Nature Protocols,2007,2(9):2233-2244
[21] Goffeau A,Barrell B G,Bussey H,Davis R W,Dujon B,Feldmann H,Galibert F,Hoheisel J D,Jacq C,Johnston M,Louis E J,Mewes H W,Murakami Y,Philippsen P,Tettelin H,Oliver S G.Life with 6 000 genes[J].Science,1996,274(5287):546,563-567
[22] Gregory T R.Macroevolution,hierarchy theory,and the C-value enigma[J].Paleobiology,2004,30:179-202
[23] Pawlowski J.Protist Evolution and Phylogeny[M].Chichester:John Wiley & Sons Ltd,2014
[24] 高胜寒,禹海英,吴双阳,王森,耿佳宁,骆迎峰,胡松年.复杂基因组测序技术研究进展[J].遗传,2018,40(11):944-963Gao S H,Yu H Y,Wu S Y,Wang S,Geng J N,Luo Y F,Hu S N.Advances of sequencing and assembling technologies for complex genomes[J].Hereditas (Beijing),2018,40(11):944-963 (in Chinese)
[2] Raper K B.The Dictyostelids[M].Princeton:Princeton University Press,1984,48-177
[3] Kirk P M,Cannon P E,Minter D W,Stalpers J A.Dictionary of the Fungi[M].10th ed.Trowbridge:Cromwell Press,2008
[4] Eichinger L,Pachebat J A,Gl?ckner G,Rajandream M A,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov B A,Rivero F,Bankier A T,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail M A,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox E C,Chisholm R L,Gibbs R,Loomis W F,Platzer M,Kay R R,Williams J,Dear P H,Noegel A A,Barrell B,Kuspa A.The genome of the social amoeba Dictyostelium discoideum[J].Nature,2005,435(7038):43-57
[5] Heidel A J,Gl?ckner G.Mitochondrial genome evolution in the social amoebae[J].Molecular Biology and Evolution,2008,25(7):1440-1450
[6] Gonzales C M,Spencer T D,Pendley S S,Welker D L.Dgp1 and Dfp1 are closely related plasmids in the Dictyostelium Ddp2 plasmid family[J].Plasmid,1999,41(2):89-96
[7] Sucgang R,Kuo A L,Tian X J,Salerno W,Parikh A,Feasley C L,Dalin E,Tu H,Huang E Y,Barry K,Lindquist E,Shapiro H,Bruce D,Schmutz J,Salamov A,Fey P,Gaudet P,Anjard C,Babu M M,Basu S,Bushmanova Y,van der Wel H,Katoh-Kurasawa M,Dinh C,Coutinho P M,Saito T,Elias M,Schaap P,Kay R R,Henrissat B,Eichinger L,Rivero F,Putnam N H,West C M,Loomis W F,Chisholm R L,Shaulsky G,Strassmann J E,Queller D C,Kuspa A,Grigoriev I V.Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum[J].Genome Biology,2011,12(2):R20
[8] Urushihara H,Kuwayama H,Fukuhara K,Itoh T,Kagoshima H,Shin-I T,Toyoda A,Ohishi K,Taniguchi T,Noguchi H,Kuroki Y,Hata T,Uchi K,Mohri K,King J S,Insall R H,Kohara Y,Fujiyama A.Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation[J].BMC Genomics,2015,16:80
[9] He X L,Li Y.A new species of Dictyostelium[J].Mycotaxon,2008,106:379-383
[10] Liu P,Li Y.Dictyostelids from Heilongjiang Province,China[J].Nova Hedwigia,2012,94(1):265-270
[11] Liu P,Li Y.Dictyostelids from Jilin Province,China I[J].Phytotaxa,2014,183(4):279-283
[12] Liu P,Li Y.Dictyostelids from Jilin Province,China II[J].Phytotaxa,2017,323(1):77-82
[13] Liu P,Zou Y,Li S,Stephenson S L,Wang Q,Li Y.Two new species of dictyostelid cellular slime molds in high-elevation habitats on the Qinghai-Tibet Plateau,China[J].Scientific Reports,2019,9:5
[14] Greilhuber J,Dole?el J,Lysák M A,Bennett M D.The origin,evolution and proposed stabilization of the terms ‘genome size’ and ‘C-value’ to describe nuclear DNA contents[J].Annals of Botany,2005,95(1):255-260
[15] 陈丙义,李金凤,霍恒志,万春雁,章镇,乔玉山,糜林.6种野生草莓基因组大小估算[J].果树学报,2015,32(1):51-56Chen B Y,Li J F,Huo H Z,Wan C Y,Zhang Z,Qiao Y S,Mi L.Estimation of genome size in six wild strawberry species[J].Journal of Fruit Science,2015,32(1):51-56 (in Chinese)
[16] 李秋实,徐江,朱英杰,孙超,宋经元,陈士林.基于流式细胞技术的灵芝基因组大小估测[J].菌物学报,2013,32(5):899-906Li Q S,Xu J,Zhu Y J,Sun C,Song J Y,Chen S L.Estimation of the genome size of Ganoderma lucidum based on a flow cytometric analysis[J].Mycosystema,2013,32(5):899-906 (in Chinese)
[17] 逄洪波,高秋,李玥莹,马纯艳,刘宏鑫,杨成瑶,吕瑞雪.利用流式细胞仪测定鬼针草基因组大小[J].基因组学与应用生物学,2016,35(7):1800-1804Pang H B,Gao Q,Li Y Y,Ma C Y,Liu H X,Yang C Y,Lv R X.Genome size estimation of Bidens bipinnata L by using flow cytometry[J].Genomics and Applied Biology,2016,35(7):1800-1804 (in Chinese)
[18] 王亚之,李秋实,陈士林,孙超,宋经元.基于流式细胞分析技术的茯苓基因组大小测定[J].世界科学技术:中医药现代化,2010,12(3):452-456Wang Y Z,Li Q S,Chen S L,Sun C,Song J Y.Flow cytometric analysis of nuclear DNA content in Poria cocos[J].World Science and Technology (Modernization of Traditional Chinese Medicine and Materia Medica),2010,12(3):452-456 (in Chinese)
[19] 李聪.酿酒酵母培养条件及发酵培养基的优化[J].中国农学通报,2014,30(9):302-306Li C.Optimization of culturing conditions and fermentation culture media of Saccharomy cescerevisiae[J].Chinese Agricultural Science Bulletin,2014,30(9):302-306 (in Chinese)
[20] Dole?el J,Greilhuber J,Suda J.Estimation of nuclear DNA content in plants using flow cytometry[J].Nature Protocols,2007,2(9):2233-2244
[21] Goffeau A,Barrell B G,Bussey H,Davis R W,Dujon B,Feldmann H,Galibert F,Hoheisel J D,Jacq C,Johnston M,Louis E J,Mewes H W,Murakami Y,Philippsen P,Tettelin H,Oliver S G.Life with 6 000 genes[J].Science,1996,274(5287):546,563-567
[22] Gregory T R.Macroevolution,hierarchy theory,and the C-value enigma[J].Paleobiology,2004,30:179-202
[23] Pawlowski J.Protist Evolution and Phylogeny[M].Chichester:John Wiley & Sons Ltd,2014
[24] 高胜寒,禹海英,吴双阳,王森,耿佳宁,骆迎峰,胡松年.复杂基因组测序技术研究进展[J].遗传,2018,40(11):944-963Gao S H,Yu H Y,Wu S Y,Wang S,Geng J N,Luo Y F,Hu S N.Advances of sequencing and assembling technologies for complex genomes[J].Hereditas (Beijing),2018,40(11):944-963 (in Chinese)