杂豆膳食纤维对大鼠卵巢衰老的影响
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摘要
探讨不同剂量杂豆膳食纤维对大鼠卵巢衰老的影响。结果表明杂豆膳食纤维对雌性大鼠卵巢衰老有良好的抑制效果,可提高血清中雌性激素含量,修复受损卵巢组织,且其改善衰老大鼠机体指标的能力及修复受损卵巢组织的效果与剂量呈正相关。实验方法:实验大鼠饲喂普通饲料,适应性喂养7 d后,分为6组,连续灌胃28 d。测定各组大鼠体重、卵巢脏体比、血清中促卵泡激素(FSH)、促黄体生成素(LH)、雌二醇(E_2)及抗苗勒氏管激素(AMH)含量,并取卵巢组织制作H&E染色病理学切片进行观察。对照组及中、高剂量杂豆膳食纤维给药组大鼠血清FSH及LH含量均较模型组大鼠下降,差异极显著(P<0.01),低剂量杂豆膳食纤维给药组差异显著(P<0.05);对照组大鼠血清E_2及AMH含量较模型组大鼠显著升高,除低剂量组外,不同剂量杂豆膳食纤维给药组大鼠血清E_2含量均较模型组大鼠升高,差异极显著(P<0.01)。卵巢病理学切片结果表明,各灌胃给药组大鼠卵巢组织衰老损伤程度明显减轻,且杂豆膳食纤维对雌性大鼠衰老造成卵巢损伤的改善效果与剂量呈正相关。
Miscellaneous Bean dietary fiber has a good inhibitory effect on ovarian aging in female rats. It can repair the body due to oxidative aging caused by oxidative stress injury, and also can improve the serum estrogen content to repair damaged ovarian tissue. At last, its improvement of aging rat body index and the effect of repairing damaged ovarian tissue was positively correlated with the dose. To investigate the effects of different doses of Miscellaneous Bean dietary fiber on ovarium senility of rats, the rats were fed with normal diet for 7 days, and then divided into 6 groups,28-day period continuous by gavage. Menstruating the body weight, the organ coefficient of ovary of each group rats.Furthermore, check the levels of Follicle-stimulating Hormone(FSH), Luteinizing Hormone(LH), Estradiol(E2), Antimullerian Hormone(AMH) of each group rats. Ovarian tissue was stained and pathological H&E sections were observed.The levels of serum FSH and LH in the control group, middle and high dose Miscellaneous Bean dietary fiber group were significantly lower than those in the model group(P<0.01), and the difference was significant(P<0.05). The contents of serum E2 and AMH in the control group were significantly higher than those in the model group. In addition to the low dose group, the levels of serum E2 in the rats of different doses of Miscellaneous Bean dietary fiber were both significantly higher than those in the model group(P<0.01). The results of ovarian pathology showed that the degree of senility was significantly decreased in female rats, and the number of follicles was significantly increased. The number of granules in the follicles was significantly decreased, and the Miscellaneous Bean dietary fiber causes ovarian injury. The improvement effect was positively correlated with the dose.
Miscellaneous Bean dietary fiber has a good inhibitory effect on ovarian aging in female rats. It can repair the body due to oxidative aging caused by oxidative stress injury, and also can improve the serum estrogen content to repair damaged ovarian tissue. At last, its improvement of aging rat body index and the effect of repairing damaged ovarian tissue was positively correlated with the dose. To investigate the effects of different doses of Miscellaneous Bean dietary fiber on ovarium senility of rats, the rats were fed with normal diet for 7 days, and then divided into 6 groups,28-day period continuous by gavage. Menstruating the body weight, the organ coefficient of ovary of each group rats.Furthermore, check the levels of Follicle-stimulating Hormone(FSH), Luteinizing Hormone(LH), Estradiol(E2), Antimullerian Hormone(AMH) of each group rats. Ovarian tissue was stained and pathological H&E sections were observed.The levels of serum FSH and LH in the control group, middle and high dose Miscellaneous Bean dietary fiber group were significantly lower than those in the model group(P<0.01), and the difference was significant(P<0.05). The contents of serum E2 and AMH in the control group were significantly higher than those in the model group. In addition to the low dose group, the levels of serum E2 in the rats of different doses of Miscellaneous Bean dietary fiber were both significantly higher than those in the model group(P<0.01). The results of ovarian pathology showed that the degree of senility was significantly decreased in female rats, and the number of follicles was significantly increased. The number of granules in the follicles was significantly decreased, and the Miscellaneous Bean dietary fiber causes ovarian injury. The improvement effect was positively correlated with the dose.
引文
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[23]王颖,徐炳政,王欣卉,等.酵母源金属硫蛋白对铅致肾脂质过氧化损伤的拮抗作用[J].中国食品学报,2016,16(6):37-43.
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[25]迟晓星,张涛,甄井龙,等.金雀异黄素调节围绝经期小鼠血清抑制素与性激素水平的研究[J].中国食品学报,2016,16(7):47-51.
[26]VIGIER B,WATRIN F,MAGRE S,et al.Effect of anti-Mullerian hormone(AMH)upon structure and germ-cell population of fetal rat ovaries.An experimental freemartin effect[J].Annals of the New York Academy of Sciences,2010,513(1):288-291.
[27]JIM魪NEZ-ESCRIG A,G魷MEZ-ORD魷譙EZ E,TENORIO M D,et al.Antioxidant and prebiotic effects of dietary fiber co-travelers from sugar Kombu in healthy rats[J].Journal of Applied Phycology,2013,25(2):503-512.
[28]ZHANG H,ADHIKARI D,ZHENG W,et al.Combating ovarian aging depends on the use of existing ovarian follicles,not on putative oogonial stem cells[J].Reproduction,2013,146(6):229-233.
[29]迟晓星,陈容,张涛,等.金雀异黄素对PCOS高雄血症大鼠子宫和卵巢形态学的影响[J].中国食品学报,2013,13(5):11-16.
[30]ZHANG J,FANG L,LU Z,et al.Are sirtuins markers of ovarian aging?[J].Gene,2016,575(2):680-686.
[2]BLEIL M E,ADLER N E,PASCH L A,et al.Depressive symptomatology,psychological stress,and ovarian reserve:a role for psychological factors in ovarian aging[J].Menopause-the Journal of the North American Menopause Society,2012,19(11):1176-1185.
[3]谢明勇,胡晓鹃,聂少平.植物雌激素早期摄入对个体生殖发育的影响[J].中国食品学报,2012,12(7):1-8.
[4]王爱梅,李弋,陈亚奇.葛根异黄酮对衰老大鼠卵巢形态和功能的影响[J].中国妇幼保健,2011,26(36):5786-5788.
[5]刘天祎,潘道东.干酪乳杆菌发酵酸乳对小鼠抗衰老功能的影响[J].中国食品学报,2013,13(3):21-25.
[6]罗爱月,丁婷,王世宣.卵巢衰老诊断的研究现状[J].中国妇幼保健,2010,25(35):5336-5338.
[7]YOUNIS J S.Ovarian aging[J].Current Opinion in Obstetrics&Gynecology,2011,23(6):427-434.
[8]IGLESIAS C,BANKER M,MAHAJAN N,et al.Ethnicity as a determinant of ovarian reserve:differences in ovarian aging between Spanish and Indian women[J].Fertility and Sterility,2014,102(1):244-249.
[9]SHAPOVALOV F A,BOROVAYA T G,KRUGLIKOV G G,et al.The morphology of the rat ovarian network during reproduction and aging[J].Bulletin of Experimental Biology and Medicine,2013,154(6):802-804.
[10]PAREDES A,RICU M,LARA H.Participation of kisspeptin in the follicular development during ovarian aging in the rat[J].Reproductive Biology,2013,13(3):44-45.
[11]CHEN Z G,LUO L L,XU J J,et al.Effects of plant polyphenols on ovarian follicular reserve in aging rats[J].Biochemistry&Cell Biology,2010,88(4):737-745.
[12]扈晓杰,韩冬,李铎.膳食纤维的定义、分析方法和摄入现状[J].中国食品学报,2011,11(3):133-137.
[13]HIPSLEY E H.Dietary“fibre”and pregnancy toxcemia[J].International Journal of Food Sciences&Nutrition,1953,7(4):168-173.
[14]TAKUJI SHIRASAWA.Dietary fiber and anti-aging[J].The Food Industry,2012,55(13):82-84.
[15]TANAKA S,YOSHIMURA Y,KAMADA C,et al.Intakes of dietary fiber,vegetables,and fruits and incidence of cardiovascular disease in japanese patients with type 2 diabetes[J].Diabetes Care,2013,36(12):3916-3922.
[16]TURNER N D,LUPTON J R.Dietary Fiber[J].Advances in Nutrition,2011,2(2):151-152.
[17]LATTIMER J M,HAUB M D.Effects of dietary fiber and its components on metabolic health[J].Nutrients,2010,2(12):1266-1289.
[18]ZHANG M,MU T,HAN J.Effects of sweet potato dietary fiber on the quality and aging of steamed bread[J].Journal of Jiangsu Normal University,2016,34(4):20-24.
[19]STEIN A C,COHEN R D.Dietary fiber intake and crohn’s disease[J].Gastroenterology,2014,146(4):1133-1133.
[20]司建志,王硕,周小雷,等.中药黄酮类成分延缓衰老作用研究进展[J].世界中西医结合杂志,2015(5):734-736.
[21]LI L.Study on the relationship between allogeneic fresh,cold storage neonatal rats ovary and aged rats ovarian specimens stress relaxation characteristics[J].Shaanxi Medical Journal,2015,4:390-391
[22]王颖,王欣卉,徐炳政,等.酵母源金属硫蛋白对慢性汞中毒小鼠氧化损伤的修复作用[J].食品科学,2016,37(15):242-246.
[23]王颖,徐炳政,王欣卉,等.酵母源金属硫蛋白对铅致肾脂质过氧化损伤的拮抗作用[J].中国食品学报,2016,16(6):37-43.
[24]ROSENCRANTZ M A,WACHS D S,COFFLER M S,et al.Comparison of inhibin B and estradiol responses to intravenous FSH in women with polycystic ovary syndrome and normal women[J].Human Reproduction,2010,25(1):198-203.
[25]迟晓星,张涛,甄井龙,等.金雀异黄素调节围绝经期小鼠血清抑制素与性激素水平的研究[J].中国食品学报,2016,16(7):47-51.
[26]VIGIER B,WATRIN F,MAGRE S,et al.Effect of anti-Mullerian hormone(AMH)upon structure and germ-cell population of fetal rat ovaries.An experimental freemartin effect[J].Annals of the New York Academy of Sciences,2010,513(1):288-291.
[27]JIM魪NEZ-ESCRIG A,G魷MEZ-ORD魷譙EZ E,TENORIO M D,et al.Antioxidant and prebiotic effects of dietary fiber co-travelers from sugar Kombu in healthy rats[J].Journal of Applied Phycology,2013,25(2):503-512.
[28]ZHANG H,ADHIKARI D,ZHENG W,et al.Combating ovarian aging depends on the use of existing ovarian follicles,not on putative oogonial stem cells[J].Reproduction,2013,146(6):229-233.
[29]迟晓星,陈容,张涛,等.金雀异黄素对PCOS高雄血症大鼠子宫和卵巢形态学的影响[J].中国食品学报,2013,13(5):11-16.
[30]ZHANG J,FANG L,LU Z,et al.Are sirtuins markers of ovarian aging?[J].Gene,2016,575(2):680-686.