动物园圈养大熊猫毕氏肠微孢子虫分子流行病学调查
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摘要
为了解各地动物园圈养大熊猫的毕氏肠微孢子虫感染情况及其人畜共患风险,采用基于ITS基因的巢氏PCR方法,对江苏、福建、浙江等10个省(市、自治区)的动物园圈养大熊猫毕氏肠微孢子虫的感染情况及基因分型进行调查和检测。在31份大熊猫粪便样品中,共检测到毕氏肠微孢子虫阳性14份,总感染率45.2%,检测出SC02(13/14)和Peru6(1/14)两种毕氏肠微孢子虫基因型。种系发育分析表明,SC02和Peru6都属于具有人畜共患潜能的group 1b。本实验首次发现SC02基因型毕氏肠微孢子虫感染大熊猫,表明大熊猫可能作为毕氏肠微孢子虫的储存宿主引起人微孢子虫病,拓宽了基因型SC02的毕氏肠微孢子虫宿主范围。
In order to reveal the prevalence of Enterocytozoon bieneusi in captive giant panda and assess its zoonotic potential,we surveyed 31 fecal samples from captive giant panda based on nested PCR amplification of ITS gene of E. bieneusi. A total of 14 samples were positive for E. bieneusi,with an infection rate of 45. 2%. Two genotypes were identified as SC02( 13/14) and Peru6( 1/14). Phylogenetic analysis revealed that these genotypes belonged to group 1 b which had zoonotic potential. Giant panda could spread E. bieneusi to humans and lead to human microsporidiosis. The first report of SC02 in giant panda broadened the understanding of E. bieneusi of hosts.
In order to reveal the prevalence of Enterocytozoon bieneusi in captive giant panda and assess its zoonotic potential,we surveyed 31 fecal samples from captive giant panda based on nested PCR amplification of ITS gene of E. bieneusi. A total of 14 samples were positive for E. bieneusi,with an infection rate of 45. 2%. Two genotypes were identified as SC02( 13/14) and Peru6( 1/14). Phylogenetic analysis revealed that these genotypes belonged to group 1 b which had zoonotic potential. Giant panda could spread E. bieneusi to humans and lead to human microsporidiosis. The first report of SC02 in giant panda broadened the understanding of E. bieneusi of hosts.
引文
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[12]DENG L,LI W,ZHONG Z,et al.Multi-locus genotypes of Enterocytozoon bieneusi in captive Asiatic black bears in southwestern China:high genetic diversity,broad host range,and zoonotic potential[J].PLo S One,2017,12(2):e0171772.
[13]LI W,DENG L,YU X,et al.Multilocus genotypes and broad host-range of Enterocytozoon bieneusi in captive wildlife at zoological gardens in China[J].Parasites&Vectors,2016,9:395.
[14]DENG L,LI W,ZHONG Z,et al.Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China[J].Parasites&Vectors,2016,9:561.
[15]ZHAO W,YU S,YANG Z,et al.Genotyping of Enterocytozoon bieneusi(Microsporidia)isolated from various birds in China[J].Infection,Genetics and Evolution,2016,40:151-154.
[16]ZHAO W,ZHANG W,YANG D,et al.Prevalence of Enterocytozoon bieneusi and genetic diversity of ITS genotypes in sheep and goats in China[J].Infection,Genetics and Evolution,2015,32:265-270.
[2]MATHIS A,WEBER R,DEPLAZES P.Zoonotic potential of the microsporidia[J].Clinical Microbiology Reviews,2005,18(3):423-445.
[3]张旭,郝志全,尹继刚,等.微孢子虫病研究现状[J].国际医学寄生虫病杂志,2010,37(2):114-119.ZHANG X,HAO Z Q,YIN J G,et al.The recent advancement of microsporidiosis[J].International Journal of Medical Parasitic Diseases,2010,37(2):114-119.(in Chinese with Engilish abstract)
[4]DIDIER E S,WEISS L M.Microsporidiosis:current status[J].Current Opinion in Infectious Diseases,2006,19(5):485-492.
[5]刁瑞南,肖立华,宋铭忻,等.毕氏肠微孢子虫基因分型及公共卫生学重要性[J].中国预防兽医学报,2014,36(12):988-991.DIAO R N,XIAO L H,SONG M X,et al.The genetypes of Enterocytozoon bieneusi and its importance for the pubilic health security[J].Chinese Journal of Preventive Veterinary Medicine,2014,36(12):988-991.(in Chinese)
[6]李威,王利勤,钟志军,等.一株金丝猴源毕氏肠微孢子虫的基因鉴定[J].中国兽医科学,2016,46(8):986-990.LI W,WANG L Q,ZHONG Z J,et al.Genetic identification of a golden monkey-derived Enterocytozoon bieneusi[J].Chinese Veterinary Science,2016,46(8):986-990.(in Chinese with Engilish abstract)
[7]周紫峣,钟志军,周潇潇,等.大熊猫肠道菌群的研究进展[J].微生物学通报,2016,43(6):1366-1371.ZHOU Z Y,ZHONG Z J,ZHOU X X,et al.Giant panda intestinal bacteria:a review[J].Microbiology China,2016,43(6):1366-1371.(in Chinese with Engilish abstract)
[8]宋军科,王正皓,王会宝,等.秦岭大熊猫槽盘吸虫的鉴定与遗传进化分析[J].中国兽医学报,2016,36(4):563-566.SONG J K,WANG Z H,WANG H B,et al.Identification and phylogenetic analysis of Ogmocotyle sp.from giant panda in Qinling Mountain[J].Chines Journal of Veterinary Science,2016,36(4):563-566.(in Chinese with Engilish abstract)
[9]SULAIMAN I M,FAYER R,LAL A A,et al.Molecular characterization of microsporidia indicates that wild mammals harbor host-adapted Enterocytozoon spp.as well as humanpathogenic Enterocytozoon bieneusi[J].Applied and Environmental Microbiology,2003,69(8):4495-4501.
[10]DIDIER E S,WEISS L M.Microsporidiosis:not just in AIDS patients[J].Current Opinion in Infectious Diseases,2011,24(5):490-495.
[11]TIAN G R,ZHAO G H,DU S Z,et al.First report of Enterocytozoon bieneusi from giant pandas(Ailuropoda melanoleuca)and red pandas(Ailurus fulgens)in China[J].Infection,Genetics and Evolution,2015,34:32-35.
[12]DENG L,LI W,ZHONG Z,et al.Multi-locus genotypes of Enterocytozoon bieneusi in captive Asiatic black bears in southwestern China:high genetic diversity,broad host range,and zoonotic potential[J].PLo S One,2017,12(2):e0171772.
[13]LI W,DENG L,YU X,et al.Multilocus genotypes and broad host-range of Enterocytozoon bieneusi in captive wildlife at zoological gardens in China[J].Parasites&Vectors,2016,9:395.
[14]DENG L,LI W,ZHONG Z,et al.Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China[J].Parasites&Vectors,2016,9:561.
[15]ZHAO W,YU S,YANG Z,et al.Genotyping of Enterocytozoon bieneusi(Microsporidia)isolated from various birds in China[J].Infection,Genetics and Evolution,2016,40:151-154.
[16]ZHAO W,ZHANG W,YANG D,et al.Prevalence of Enterocytozoon bieneusi and genetic diversity of ITS genotypes in sheep and goats in China[J].Infection,Genetics and Evolution,2015,32:265-270.