低温对茄子幼苗生理特性的影响及耐冷性指标的筛选
|
摘要
以茄子幼苗为材料,探讨低温胁迫对茄子幼苗生理生化特性的影响,并对各指标进行相关性分析。结果表明:低温胁迫使茄子幼苗叶片光合色素含量降低;增加了茄子幼苗丙二醛(MDA)、脯氨酸、可溶性蛋白和可溶性糖含量,提高了超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,且电导率值显著增大。恢复常温,光合色素含量都有不同程度升高,但低于对照,其他指标均降低,仍高于对照。相关性结果分析表明:所测各指标之间呈极显著正相关,且电导率与丙二醛、可溶性糖之间相关系数最大。研究结果表明,10℃/5℃(昼/夜)低温环境严重影响茄子幼苗的正常代谢,低于10℃/5℃(昼/夜)茄子幼苗保护系统破坏严重,不能够恢复正常生长,电导率、丙二醛、可溶性糖含量可作为茄子幼苗耐低温能力可靠的鉴定指标。
Effects of low temperature stress on the physiological and biochemical properties as well as the antioxidant enzyme activities of the eggplant seedlings and the correlation among the indicators for the cold-tolerance of the plants were studied.The results indicated that cold stress reduced the content of chlorophyll in leaves but increased those of MDA,proline,soluble protein and soluble sugar in the seedlings.Meanwhile,the electric conductivity and the activities of SOD,POD and CAT also increased significantly.By moving the stressed seedlings back into normal temperature,the chlorophyll content increased slightly but remained lower than control,while the other indicators declined to the levels still higher than those of control.There were ery significant correlations existed among the indicators.The highest correlation coefficient was found between the electric conductivity and the MDA or soluble sugar content.It appeared that a day/night low temperature exposure at 10℃/5℃ would irreversibly damage the normal metabolism of the seedlings.The electric conductivity,MDA and soluble sugar seemed to be appropriate for evaluating the cold-tolerance of eggplant seedlings.
Effects of low temperature stress on the physiological and biochemical properties as well as the antioxidant enzyme activities of the eggplant seedlings and the correlation among the indicators for the cold-tolerance of the plants were studied.The results indicated that cold stress reduced the content of chlorophyll in leaves but increased those of MDA,proline,soluble protein and soluble sugar in the seedlings.Meanwhile,the electric conductivity and the activities of SOD,POD and CAT also increased significantly.By moving the stressed seedlings back into normal temperature,the chlorophyll content increased slightly but remained lower than control,while the other indicators declined to the levels still higher than those of control.There were ery significant correlations existed among the indicators.The highest correlation coefficient was found between the electric conductivity and the MDA or soluble sugar content.It appeared that a day/night low temperature exposure at 10℃/5℃ would irreversibly damage the normal metabolism of the seedlings.The electric conductivity,MDA and soluble sugar seemed to be appropriate for evaluating the cold-tolerance of eggplant seedlings.
引文
[1]刘黎军.低温弱光对日光温室茄子生长及生理生化功能的影响[D].长春:吉林农业大学,2012:46-59.
[2]吴雪霞,陈建林,查丁石.低温胁迫对茄子幼苗叶片叶绿素荧光特性和能量耗散的影响[J].植物营养与肥料学报,2009,15(1):164-169.
[3]敖礼林.蔬菜的低温冷害及其综合防护[J].科学种养,2016,31(12):34.
[4]董爱玲,颉建明,李杰,等.低温驯化对低温胁迫下茄子幼苗生理活性的影响[J].甘肃农业大学学报,2017,28(1):74-79,86.
[5]李合生.现代植物生理学[M].第3版.北京:高等教育出版社,2012:364-367.
[6]高青海,吴燕,徐坤,高辉远.茄子嫁接苗根系对低温环境胁迫的响应[J].应用生态学报,2006,17(3):3390-3394.
[7]隽加香.低温胁迫下番茄植株光合及呼吸代谢特性的研究[D].哈尔滨:东北农业大学,2015:38-42.
[8]张子山.低温弱光胁迫下黄瓜叶片光系统Ⅰ与光系统Ⅱ的相互作用[D].泰安:山东农业大学,2013,25-49.
[9]王春萍,黄启中,雷开荣,等.低温弱光下辣椒幼苗叶绿素荧光特性及其与品种耐性的关系[J].园艺学报,2015,42(9):1798-1806.
[10]吴雪霞,陈建林,查丁石.低温胁迫对茄子幼苗叶片光合特性的影响[J].华北农学报,2008,23(5):185-189.
[11]李烨,谢立波,陈永琴,等.外源氯化钙和脱落酸处理对茄子低温胁迫相关指标的影响[J].北方园艺,2012,35(7):22-25.
[12]张晓艳.砧穗互作与茄子嫁接苗抗冷性的关系[D].泰安:山东农业大学,2009:12-36.
[13]郭守鹏,杨君娇,马村,等.低温胁迫对不同类型茄子叶片耐寒性生理指标的影响[J].现代园艺,2018,3(5):8-10.
[14]张素勤,耿广东,程智慧.低温胁迫程度对茄子幼苗生理特性的影响[J].山地农业生物学报,2008,27(2):119-122.
[15]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:142-143.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-168.
[17]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:167-173.
[18]邵怡若,许建新,薛立,等.低温胁迫时间对4种幼苗生理生化及光合特性的影响[J].生态学报,2013,33(14):4237-4247.
[19]张永平,姚雪琴,杨少军,等.低温胁迫及恢复对厚皮甜瓜光合作用和抗氧化特性的影响[J].上海农业学报,2017,33(1):41-49.
[20]欧俊梅,王治斌,陶军,等.低温胁迫下川西北冬小麦叶绿素含量变化规律研究[J].安徽农学通报,2017,23(Z1):11-12,34.
[21]闫世江,张继宁,刘洁.茄子幼苗耐低温性生理机制研究[J].西北植物学报,2011,31(12):2498-2502.
[22]隽加香,蒋欣梅,于锡宏.低温胁迫对番茄品种资源苗期抗冷性的影响[J].东北农业大学学报,2010,41(8):28-33.
[23]包崇来,杜黎明,胡天华,等.茄子耐低温材料的筛选及其耐低温生理响应研究[J].植物遗传资源学报,2013,14(6):1161-1166.
[24]田丹青,葛亚英,潘刚敏,等.低温胁迫对3个红掌品种叶片形态和生理特性的影响[J].园艺学报,2011,38(6):1173-1179.
[25]许英,陈建华,朱爱国,等.低温胁迫下植物响应机理的研究进展[J].中国麻业科学,2015,37(1):40-49.
[26]吴雪霞,查丁石,邰翔.低温胁迫对茄子幼苗生长、抗氧化酶活性和渗透调节物质的影响[J].江苏农业学报,2008,24(4):471-475.
[27]魏秀清,许玲,章希娟,等.莲雾对低温胁迫的生理响应及抗寒性分析[J].果树学报,2016.33(1):73-80.
[2]吴雪霞,陈建林,查丁石.低温胁迫对茄子幼苗叶片叶绿素荧光特性和能量耗散的影响[J].植物营养与肥料学报,2009,15(1):164-169.
[3]敖礼林.蔬菜的低温冷害及其综合防护[J].科学种养,2016,31(12):34.
[4]董爱玲,颉建明,李杰,等.低温驯化对低温胁迫下茄子幼苗生理活性的影响[J].甘肃农业大学学报,2017,28(1):74-79,86.
[5]李合生.现代植物生理学[M].第3版.北京:高等教育出版社,2012:364-367.
[6]高青海,吴燕,徐坤,高辉远.茄子嫁接苗根系对低温环境胁迫的响应[J].应用生态学报,2006,17(3):3390-3394.
[7]隽加香.低温胁迫下番茄植株光合及呼吸代谢特性的研究[D].哈尔滨:东北农业大学,2015:38-42.
[8]张子山.低温弱光胁迫下黄瓜叶片光系统Ⅰ与光系统Ⅱ的相互作用[D].泰安:山东农业大学,2013,25-49.
[9]王春萍,黄启中,雷开荣,等.低温弱光下辣椒幼苗叶绿素荧光特性及其与品种耐性的关系[J].园艺学报,2015,42(9):1798-1806.
[10]吴雪霞,陈建林,查丁石.低温胁迫对茄子幼苗叶片光合特性的影响[J].华北农学报,2008,23(5):185-189.
[11]李烨,谢立波,陈永琴,等.外源氯化钙和脱落酸处理对茄子低温胁迫相关指标的影响[J].北方园艺,2012,35(7):22-25.
[12]张晓艳.砧穗互作与茄子嫁接苗抗冷性的关系[D].泰安:山东农业大学,2009:12-36.
[13]郭守鹏,杨君娇,马村,等.低温胁迫对不同类型茄子叶片耐寒性生理指标的影响[J].现代园艺,2018,3(5):8-10.
[14]张素勤,耿广东,程智慧.低温胁迫程度对茄子幼苗生理特性的影响[J].山地农业生物学报,2008,27(2):119-122.
[15]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:142-143.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-168.
[17]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:167-173.
[18]邵怡若,许建新,薛立,等.低温胁迫时间对4种幼苗生理生化及光合特性的影响[J].生态学报,2013,33(14):4237-4247.
[19]张永平,姚雪琴,杨少军,等.低温胁迫及恢复对厚皮甜瓜光合作用和抗氧化特性的影响[J].上海农业学报,2017,33(1):41-49.
[20]欧俊梅,王治斌,陶军,等.低温胁迫下川西北冬小麦叶绿素含量变化规律研究[J].安徽农学通报,2017,23(Z1):11-12,34.
[21]闫世江,张继宁,刘洁.茄子幼苗耐低温性生理机制研究[J].西北植物学报,2011,31(12):2498-2502.
[22]隽加香,蒋欣梅,于锡宏.低温胁迫对番茄品种资源苗期抗冷性的影响[J].东北农业大学学报,2010,41(8):28-33.
[23]包崇来,杜黎明,胡天华,等.茄子耐低温材料的筛选及其耐低温生理响应研究[J].植物遗传资源学报,2013,14(6):1161-1166.
[24]田丹青,葛亚英,潘刚敏,等.低温胁迫对3个红掌品种叶片形态和生理特性的影响[J].园艺学报,2011,38(6):1173-1179.
[25]许英,陈建华,朱爱国,等.低温胁迫下植物响应机理的研究进展[J].中国麻业科学,2015,37(1):40-49.
[26]吴雪霞,查丁石,邰翔.低温胁迫对茄子幼苗生长、抗氧化酶活性和渗透调节物质的影响[J].江苏农业学报,2008,24(4):471-475.
[27]魏秀清,许玲,章希娟,等.莲雾对低温胁迫的生理响应及抗寒性分析[J].果树学报,2016.33(1):73-80.