不同配方对京薯6号组培苗黄化及生长发育的影响
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摘要
以大量元素、有机元素、铁盐、糖类、激素6-BA为因素,采用L16(45)五因素四水平的正交试验设计,观测不同组合基质配方对京薯6号组培苗的黄化率、成苗率和生长发育的影响。结果表明,基质配方组合为大量元素4 mL+有机元素2.0 mL+铁盐1.0 mL+糖类8 g/L+6-BA 0.1 mg/L,京薯6号黄化率最低(11.79%)、成苗率较高(95.00%),综合评价最优;综合评价最差的组合为大量元素1 mL+有机元素2 mL+铁盐2 mL+糖类6 g/L+6-BA 1.0 mg/L,京薯6号黄化率为70.94%。由极差和方差分析结果可知,激素6-BA对京薯6号黄化的影响显著高于其他因素。在一定的激素6-BA浓度范围内,理论最优和最差基本组合下黄化率均呈"S"型曲线变化,两者曲线有相交点,最优基本组合下的黄化率比最差基本组合下的黄化率要高。
The orthogonal design of L16(45)with five factors(macro elements,organic elements,ferric salt,sucrose,and the hormone 6-BA)and four levels was used to observe the effects of different formulations on the yellowing rate,seedling formation rate and growth and development of Jingshu 6 tissue culture seedlings.The results showed that formulations(large element 4 mL+organic element 2.0 mL+iron salt 1.0 mL+sugar 8 g/L+6-BA 0.1 mg/L) was obtained with the lowest yellowing rate(11.79%) and the highest seedling rate(95.00%).The worst formulation was large element 1 mL+organic element 2 mL+iron salt 2 m L+sugar 6 g/L+6-BA 1.0 mg/L,and the yellowing rate was 70.94%.Range R and variance analysis showed that the effect of the hormone 6-BA on the yellowing of Jingshu 6 was significantly higher than that of other factors. Within a certain range of hormone 6-BA concentration,the yellowing rate under both the theoretical optimal and the worst basic formulations showed a "S" type curve change,and the two curves had intersections. The yellowing rate under the optimal basic combination was higher than that under the worst basic combination.
The orthogonal design of L16(45)with five factors(macro elements,organic elements,ferric salt,sucrose,and the hormone 6-BA)and four levels was used to observe the effects of different formulations on the yellowing rate,seedling formation rate and growth and development of Jingshu 6 tissue culture seedlings.The results showed that formulations(large element 4 mL+organic element 2.0 mL+iron salt 1.0 mL+sugar 8 g/L+6-BA 0.1 mg/L) was obtained with the lowest yellowing rate(11.79%) and the highest seedling rate(95.00%).The worst formulation was large element 1 mL+organic element 2 mL+iron salt 2 m L+sugar 6 g/L+6-BA 1.0 mg/L,and the yellowing rate was 70.94%.Range R and variance analysis showed that the effect of the hormone 6-BA on the yellowing of Jingshu 6 was significantly higher than that of other factors. Within a certain range of hormone 6-BA concentration,the yellowing rate under both the theoretical optimal and the worst basic formulations showed a "S" type curve change,and the two curves had intersections. The yellowing rate under the optimal basic combination was higher than that under the worst basic combination.
引文
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[4]雷加容,余金龙,余敖.甘薯的组织培养[J].甘肃农业大学学报,2006(1):113-115.
[5]张雅琼,郭华春.甘薯茎尖分生组织培养的研究进展[J].中国农学通报,2005(3):74-76.
[6]孔祥生,张妙霞,郭秀璞,等.甘薯茎尖分生组织培养及快速繁殖技术研究[J].河南农业大学学报,1998(2):28-32.
[7]陈世昌,徐明辉.植物组织培养[M].3版.重庆:重庆大学出版社,2016:25-26.
[8]陈明灿.甘薯组培快繁及优化栽培技术研究[D].南京:南京农业大学,2006.
[9]李强,马代夫,李洪民,等.NAA和6-BA在甘薯组织培养中的应用研究[J].江苏农业科学,2003(4):14-16.
[10]刘杰.小桐子的组织培养及其组培苗黄化问题研究[D].昆明:西南林业大学,2009.
[11]刘水丽,杨其长.无糖培养条件下三种大豆组培苗生长差异研究[J].大豆科学,2007(2):163-166.
[12]广西壮族自治区林业科学研究院.一种消除组培苗黄化的核桃组培快繁方法:中国,CN201710311958.6[P].2017-08-04.
[13]李银国,尹克宁,王树良.果树缺铁黄化研究进展[J].热带亚热带土壤科学,1997(2):129-133.