黑果枸杞叶槲皮素的制备及其体外抗氧化活性研究
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摘要
采用超声波-微波协同提取黑果枸杞叶醇提物,利用大孔吸附树脂分离富集黑果枸杞叶总黄酮,采用Sephadex LH-20,分离纯化槲皮素。利用高效液相色谱-质谱联用法(high performance liquid chromatography and mass spectrometry,HPLC-MS)对所得的槲皮素进行结构鉴定。通过考察槲皮素的总抗氧化活性、清除DPPH自由基、ABTS+自由基、超氧阴离子自由基以及脂质过氧化体系中脂质过氧化抑制率5个指标,由此评价槲皮素的体外抗氧化活性。研究结果表明,槲皮素在5种自由基中清除ABTS+自由基能力最强,浓度在300 nmol/L～400 nmol/L时清除率达68.0%。清除DPPH自由基能力和清除超氧阴离子自由基能力基本持平。
The total flavonoids of Leaves of Lycium ruthenicum Murr. were separated and enriched by macroporous adsorption resins,and the quercetin was separated and purified by Sephadex LH-20. The structure of the quercetin was identified by high performance liquid chromatography and mass spectrometry(HPLC-MS).The antioxidant activity of the quercetin in vitro was evaluated by five factors:determination of total antioxidant capacity,scavenging effects of DPPH free radical,ABTS + free radical and super-oxide anion,and lipid peroxidation inhibition rate in lipid peroxide system. The results showed that the quercetin had the strongest ability to remove ABTS+free radical. Under the concentration of quercetin in 300 nmol/L-400 nmol/L,the clearance rate can reach 68.0 %. The quercetin had the almost same ability to clear DPPH free radical and super-oxide anion free radical.
The total flavonoids of Leaves of Lycium ruthenicum Murr. were separated and enriched by macroporous adsorption resins,and the quercetin was separated and purified by Sephadex LH-20. The structure of the quercetin was identified by high performance liquid chromatography and mass spectrometry(HPLC-MS).The antioxidant activity of the quercetin in vitro was evaluated by five factors:determination of total antioxidant capacity,scavenging effects of DPPH free radical,ABTS + free radical and super-oxide anion,and lipid peroxidation inhibition rate in lipid peroxide system. The results showed that the quercetin had the strongest ability to remove ABTS+free radical. Under the concentration of quercetin in 300 nmol/L-400 nmol/L,the clearance rate can reach 68.0 %. The quercetin had the almost same ability to clear DPPH free radical and super-oxide anion free radical.
引文
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[3]张良,张玉奎,戴荣继,等.射干叶中黄酮碳苷类化合物的药理作用研究进展[J].天然产物研究与开发,2010,22(4):728-730
[4]陈文,王湘君,张顺琦,等.响应面优化超声波辅助提取仙人掌花槲皮素[J].海南热带海洋学院学报,2018,25(5):90-100
[5]王相,范帅帅,曹文利,等.基于液质联用技术的酸枣叶中9种成分含量的同时测定[J].中药材,2018,41(1):148-151
[6]陶磊,刘周阳,韩春梅,等.液质联用对重组胰高血糖素样肽-1-Fc融合蛋白中的制品相关蛋白进行定性定量分析[J].药物分析杂志,2018,38(11):1899-1904
[7]云少君,戴玥,延莎.β-胡萝卜素和植酸对胡麻油抗氧化活性的影响[J].山西农业大学学报(自然科学版),2015,35(3):277-280
[8]张明.几种体外抗氧化检测方法的评价研究[D].西安:陕西师范大学,2010:5-7
[9]孙丽萍,王大仟,张智武.11种天然植物提取物对DPPH自由基的清除作用[J].食品科学,2009,30(1):45-47
[10]Payet B,Shum C heong Sing A,Smadja J.Assessment of antioxidant activity of cane brown sugars by ABTS and DPPH radical scavenging assays:determination of their polyphenolic and volatile constituents[J].Journal of agricultural and food chemistry,2005,53(26):10074-10079
[11]孟庆焕,王化,王洪政,等.牡丹种皮黄酮提取及对ABTS自由基清除作用[J].植物研究,2013,33(4):504-507
[12]胡贵丽,刘靖,刘勇强,等.博落回生物碱和茶籽多糖对黄羽肉鸡生长性能、血清生化指标及脂质过氧化的影响[J].动物营养学报,2018,30(11):4626-4634
[13]张良,张玉奎,戴荣继,等.射干叶中黄酮碳苷类的分离纯化与结构鉴定[J].北京理工大学学报,2010,30(10):1249-1251