不同年龄段养殖宽体沙鳅肌肉营养成分分析与评价
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  • 英文篇名:NUTRITIONAL COMPOSITION OF MUSCLE IN CULTURED SINIBOTIA REEVESAE POPULATION AT DIFFERENT AGES
  • 作者:王永明 ; 史晋绒 ; 谢碧文 ; 唐瑞 ; 王淯 ; 齐泽民
  • 英文作者:WANG Yong-Ming;SHI Jin-Rong;XIE Bi-Wen;TANG Rui;WANG Yu;QI Ze-Min;Conservation and Utilization of Fishes Resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,College of Life Science, Neijiang Normal University;
  • 关键词:宽体沙鳅 ; 肌肉 ; 营养成分 ; 氨基酸 ; 脂肪酸 ; 评价
  • 英文关键词:Sinibotia reevesae;;Muscle;;Nutrient components;;Amino acid;;Fatty acid;;Valuation
  • 中文刊名:SSWX
  • 英文刊名:Acta Hydrobiologica Sinica
  • 机构:内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室;
  • 出版日期:2018-05-15 19:21
  • 出版单位:水生生物学报
  • 年:2018
  • 期:v.42
  • 基金:四川省教育厅项目(15ZB0268);; 四川省科技支撑项目(2011NZ0075)资助~~
  • 语种:中文;
  • 页:SSWX201803012
  • 页数:8
  • CN:03
  • ISSN:42-1230/Q
  • 分类号:94-101
摘要
采用国家标准方法对1、2、3龄宽体沙鳅养殖群体的肌肉营养成分及品质进行了分析比较。结果显示:(1)各年龄组宽体沙鳅肌肉粗蛋白质和粗脂肪含量较高且随着年龄的增长呈增长趋势。其中,粗蛋白在2龄和3龄组增长不显著(P>0.05),粗脂肪增长显著(P<0.05)。(2)各年龄组肌肉氨基酸组成相同,均以鲜味特征氨基酸谷氨酸含量最高,其次为天门冬氨酸;第一限制性氨基酸均为缬氨酸;支链氨基酸与芳香族氨基酸的比值(F值)分别为2.22、2.08和2.12。(3)各年龄组宽体沙鳅脂肪酸在组成和含量上略有差异,表现为不饱和脂肪酸含量高于饱和脂肪酸含量(P<0.05);不饱和脂肪酸中单不饱和脂肪酸含量较高(P<0.05);多不饱和脂肪酸(PUFA)在2龄组最高(P<0.05)且EPA+DHA含量随年龄的增长递增(P<0.05)。综上,各年龄段宽体沙鳅肌肉营养丰富,味道鲜美,以2龄鱼体营养质量最优。
        Sinibotia reevesae, an endemic fish, live only in the upper reaches of the Yangtze River. To study nutritional composition in muscle during different growth phases, the main nutrient components in the muscles of age 1, 2 and 3 farmed(fed formulated feed) S. reevesae were analyzed, and the nutritional value and product flavor were evaluated.The general nutrient compositions were determined by routine methods, the amino acid compositions were measured with an amino acid analyzer and the fatty acid compositions by gas chromatography-mass spectrometry(GC-MS). The results showed that the contents of crude protein and crude fat in S. reevesae muscles increased with fish growth. With the increase of age, the moisture content decreased, whereas the contents of crude protein and crude fat increased. The crude protein in muscles of age-group 3 was significantly higher than that of the other two groups(P<0.05), while no significant difference was found in crude protein content between age-group 2 and 3(P>0.05). The contents of different amino acids in muscles of S. reevesae among 3 groups were stable. Glutamic acid, aspartic acid and lysine were the most abundant among 16 kinds of amino acids detected in muscles of each group. The essential amino acid index(EAAI) and the ratio of branched chain amino acid to aromatic amino acid(F value) of S. reevesae were very high. According to nutrition evaluation in amino acid score(AAS) and chemical score(CS), the first limiting amino acid for muscles of S. reevesae during different growth phases was valine acid. Totally 7 saturated fatty acids(SFA), 6 monounsaturated fatty acids(MUFA), and 7 poly-unsaturated fatty acids(PUFA) were found in dry muscles of S. reevesae from each group. The content of SFA was significantly lower than that of unsaturated fatty acids(UFA)(P<0.05), and the total content of MUFA, especially C18:1 n9 c, was significantly higher among UFA. PUFA in age-group 2 was significantly higher than that of the other two groups(P<0.05). The total content of EPA and DHA was lower than other Cobitidae fishes with the tendency to descend with the increasing age. These results indicate that S. reevesae is a kind of high nutritional value, delicious, and high quality fish, and that S. reevesae in age 2 and 3 age are great for consume because of higher contents of crude protein and crude fat, better amino acid and fatty acid compositions in muscle.
引文
[1]Gong G,Xue M,Wang J,et al.Nutrition requirement and nutritional regulation of growth and development for fish larvae[J].Chinese Journal of Animal Nutrition,2014,26(4):843-851[宫官,薛敏,王嘉,等.仔稚鱼营养需要及生长发育的营养调控.动物营养学报,2014,26(4):843-851]
    [2]Ding R H.Fish in Sichuan[M].Chengdu:Sichuan Science&Technology Publishing Press.1994,96-98[丁瑞华.四川鱼类志.成都:四川科学技术出版社.1994,96-98]
    [3]Yue X J,Wang F,Xie B W,et al.Embryonic development of Sinibotia reevesae in Tuojiang River[J].Sichuan Journal of Zoology,2011,30(3):390-393,397[岳兴建,王芳,谢碧文,等.沱江流域宽体沙鳅的胚胎发育.四川动物,2011,30(3):390-393,397]
    [4]Wang F,Yue X J,Xie B W,et al.Anatomical and histological structure of digestive system of Sinibotia reevesae[J].Sichuan Journal of Zoology,2011,30(4):569-572[王芳,岳兴建,谢碧文,等.宽体沙鳅消化系统的结构.四川动物,2011,30(4):569-572]
    [5]Xu D D,Wang Z J,Wang Y M,et al.Morphological variation between Sinibotia superciliaris and Sinibotia reevesae with notes on their validities[J].Acta Hydrobiologica Sinica,2017,41(4):827-834[徐丹丹,王志坚,王永明,等.中华沙鳅和宽体沙鳅形态差异及其物种有效性分析.水生生物学报,2017,41(4):827-834]
    [6]Wang Y M,Xie B W,Wang F,et al.Reproduction characteristics of Sinibotia reevesae in the Tuojiang River[J].Chinese Journal of Zoology,2014,49(5):699-706[王永明,谢碧文,王芳,等.沱江宽体华鳅的繁殖特性.动物学杂志,2014,49(5):699-706]
    [7]Wang Y M,Xie B W,Zou Y C,et al.Age and growth of Sinibotia reevesae in the Tuojiang River[J].Chinese Journal of Zoology,2015,50(2):221-230[王永明,谢碧文,邹远超,等.沱江宽体华鳅的年龄与生长.动物学杂志,2015,50(2):221-230]
    [8]Wang Y M,Chen Y,Hu Y,et al.Ultrastructural observation of the sperm of Sinibotia reevesae and effect of Na+,K+and Ca2+on its motility[J].Sichuan Journal of Zoology,2016,35(1):38-43[王永明,陈瑜,胡雨,等.宽体沙鳅精子超微结构Na+、K+、Ca2+对其精子活力的影响.四川动物,2016,35(1):38-43]
    [9]Xie J,Li F Y,Liu X L,et al.On effects of dietary lipid level on growth,fatty acids profiles and lipase in Sinibotia reevesae[J].Journal of Southwest China Normal University(Natural Science Edition),2013,38(11):76-83[颉江,李飞扬,刘晓玲,等.饲料脂肪水平对宽体沙鳅幼鱼生长和肌肉脂肪酸组成及脂肪酶的影响.西南师范大学学报(自然科学版),2013,38(11):76-83]
    [10]Xie J,Qin C J,Hou P,et al.The level of fatty acids of Sinibotia superciliaris and Sinibotia reevesae parent fish[J].Jiangsu Agricultural Sciences,2013,41(5):290-292[颉江,覃川杰,侯平,等.沱江宽体沙鳅和中华沙鳅亲鱼脂肪酸组成分析.江苏农业科学,2013,41(5):290-292]
    [11]FAO/WHO Ad Hoe Expert Committee.Energy and Protein Requirements[M].Rome:FAO Nutrition Meeting Report Series.1973,52
    [12]Peiiett P L,Young V R.Nutritional Evaluation of Protein Foods[M].California:The United National University Publishing Company.1980,26-29
    [13]Tang X,Xu G C,Xu P,et al.A comparison of muscle nutrient composition between wild and cultured Coilia nasus[J].Chinese Journal of Animal Nutrition,2011,23(3):514-520[唐雪,徐钢春,徐跑,等.野生与养殖刀鲚肌肉营养成分的比较分析.动物营养学报,2011,23(3):514-520]
    [14]Wen X B,Ku M M,Hu X D.Studies on nutritive composition of Leptobotia elongata Bleeker[J].Journal of Hubei Agricultural College,1996,16(4):284-288[温小波,库么梅,胡小迪.长薄鳅的营养成分研究.湖北农学院学报,1996,16(4):284-288]
    [15]Zhao Z S,Gao G Q,Yin J,et al.Studies on nutritive composition of Misgurnus anguillicaudatus and Paramisgurnus dabryanus[J].Journal of Hydroecology,1999,19(2):16-17[赵振山,高贵琴,印杰,等.泥鳅和大鳞副泥鳅营养成分分析.水生态学杂志,1999,19(2):16-17]
    [16]Li H.Comparison of nutrient components in muscles of wild and artificial cultured Triplophysa siluroides[J].Sichuan Journal of Zoology,2013,32(4):584-587[李华.野生和人工养殖似鲇高原鳅肌肉营养成分比较.四川动物,2013,32(4):584-587]
    [17]Wang S,Chen S A,Song Y,et al.Analysis of nutritional composition on the Triplophysa(Hedinichthys)yarkandensis(Day)about Tarim River[J].Journal of Hydroecology,2011,32(1):137-141[王帅,陈生熬,宋勇,等.塔里木河流域叶尔羌高原鳅营养成分分析.水生态学杂志,2011,32(1):137-141]
    [18]Pu J,Jia L,Su S Q,et al.Rate of flesh content and analysis on nutrient composition of Sinibotia superciliaris[J].Journal of Southwest University(Natural Science Edition),2014,36(3):42-48[普炯,贾砾,苏胜齐,等.中华沙鳅含肉率及肌肉营养成分分析.西南大学学报(自然科学版),2014,36(3):42-48]
    [19]Wu Y A,Liang Z Q,Yuan X P,et al.Analysis and evaluation of nutritional components in the muscle of Parabotia fasciata Dabry[J].Freshwater Fisheries,2011,41(1):87-91[伍远安,梁志强,袁希平,等.花斑副沙鳅肌肉营养成分分析与评价.淡水渔业,2011,41(1):87-91]
    [20]Cui L L,Leng Y,Miao X J,et al.Evaluation of nutrient quality and composition in the muscle of Triplophysa venusta[J].Journal of Hydroecology,2016,37(2):70-75[崔丽莉,冷云,缪祥军,等.秀丽高原鳅肌肉营养成分分析与品质评价.水生态学杂志,2016,37(2):70-75]
    [21]Tan D Q,Wang J W,Dan S G,et al.The ratio of muscle to body and analysis of the biochemical composition of muscle in Megalobrama pellegrini[J].Acta Hydrobiologica Sinica,2004,28(1):17-22[谭德清,王剑伟,但胜国,等.厚颌鲂含肉率及生化成分的分析.水生生物学报,2004,28(1):17-22]
    [22]Zhou X H,Zheng S M,Wu Q,et al.An analysis of the nutritive composition in muscle of prenant’s schizothoracin Schizothorax prenanti[J].Journal of Dalian Ocean University,2005,20(1):20-24[周兴华,郑曙明,吴青,等.齐口裂腹鱼肌肉营养成分的分析.大连水产学院学报,2005,20(1):20-24]
    [23]Meng F Y,Huang Q,Hao F Q.Comparative studies on muscle composition and blood index of Salvelinus malma at different ages[J].Journal of Fishery Sciences of China,2009,16(1):113-119[孟繁伊,黄权,郝凤奇.不同年龄花羔红点鲑肌肉成分和血液指标的比较研究.中国水产科学,2009,16(1):113-119]
    [24]Liu H Z,Luo L,Cai D L,et al.Analysis and valuation of nutrient components in the muscle of Engraulis japonicus at different growth stages[J].Journal of Nuclear Agricultural Sciences,2015,29(11):2150-2157[刘海珍,罗琳,蔡德陵,等.不同生长阶段鳀鱼肌肉营养成分分析与评价.核农学报,2015,29(11):2150-2157]
    [25]Wang K,Chen B J,Liu B,et al.Analysis on nutritive composition of muscle in wild and cultured Culter alburnus populations in Xingkai Lake at different ages[J].Journal of Fishery Sciences of China,2012,19(5):906-912[王琨,程宝晶,刘斌,等.不同年龄野生和养殖兴凯湖翘嘴鲌肌肉营养成分分析.中国水产科学,2012,19(5):906-912]
    [26]Zhao N N,Li Y,Ma J,et al.Research perspectives on characteristics of nutrient requirement in broodstock and larvae in Siluriformes[J].Fisheries Science,2016,35(3):296-301[赵宁宁,李勇,马骏,等.鲇形目亲鱼和仔稚鱼营养需求特点研究进展.水产科学,2016,35(3):296-301]
    [27]Wan S L,Huang Y T,Liu M,et al.The ratio of muscle to body and analysis of the nutritive composition of muscle in Pelteobagrus vachelli[J].Journal of Hydroecology,2008,28(3):59-61[万松良,黄永涛,刘敏,等.瓦氏黄颡鱼的含肉率及营养成分分析.水生态学杂志,2008,28(3):59-61]
    [28]Huang F,Yan A S,Xiong C X,et al.Evaluation of the nutrition and the rate of flesh in the whole body of Pelteobagrus fulvidraco Rich[J].Freshwater Fisheries,1999,29(10):3-6[黄峰,严安生,熊传喜,等.黄颡鱼的含肉率及鱼肉营养评价.淡水渔业,1999,29(10):3-6]
    [29]Sun H K,Han Y Z,Sun J F,et al.Muscle nutritional comparative of four different geographical Silurus asotus groups[J].Acta Hydrobiologica Sinica,2016,40(3):493-500[孙海坤,韩雨哲,孙建富,等.四个不同地理鲇群体肌肉营养组成的比较分析.水生生物学报,2016,40(3):493-500]
    [30]Yan A S,Xiong C X,Qian J W,et al.A study on the rate of flesh content of mandarin fish and nutritional quality of the flesh[J].Journal of Huazhong Agrieultural University,1995,14(1):80-84[严安生,熊传喜,钱健旺,等.鳜鱼含肉率及鱼肉营养价值的研究.华中农业大学学报,1995,14(1):80-84]
    [31]Wang P,Xie Q.Branched-chain amino acids as pharmacological nutrients in chronic liver disease[J].Pharmaceutical And Clinical Research,2011,19(6):481-484[王芃,谢青.支链氨基酸作为药理营养素在慢性肝病治疗中的应用.药学与临床研究,2011,19(6):481-484]
    [32]Kakazu E,Ueno Y,Kondo Y,et al.Branched chain amino acids enhance the maturation and function of myeloid dendritic cells ex vivo in patients with advanced cir-rhosis[J].Hepatology,2009,50(6):1936-1945
    [33]Ichikawa T,Naota T,Miyaaki H,et al.Effect of an oral branched chain amino acid-enriched snack in cirrhotic patients with sleep disturbance[J].Hepatology Research,2010,40(10):971-978
    [34]Rainuzzo J R,Reitan K I,J?rgensen L.Comparative study on the fatty acid and lipid composition of four marine fish larvae[J].Biochemistry and Physiology Part B:Comparative Biochemistry,1992,103(1):21-26
    [35]Zhou Z,Li Z Y.Advances on the research of nutritional requirements of larvae[J].Guizhou Journal of Animal Husbandry&Veterinary Medicine,2013,37(4):65-68[周洲,李正友.鱼类仔稚鱼营养需求研究进展.贵州畜牧兽医,2013,37(4):65-68]
    [36]Fernández-Palacios H,Norberg B,Izquierdo M,et al.Effects of Broodstock Diet on Eggs and Larvae[M].In:Holt G J(Eds.),Larval Fish Nutrition.Wiley-Blackwell,UK.2011,153-182
    [37]Li Y Y,Chen W Z,Sun Z W,et al.Effects of n-3HUFAcontent in broodstock diet on spawning per-formance and fatty acid composition of eggs and larvae in Plectorhynchus cinctus[J].Aquaculture,2005,245(1):263-272
    [38]Vassallo A R,Watanabe T,Yoshizaki G,et al.Quality of eggs and spermatozoa of rainbow trout fed an n-3 essential fatty acid-deficient diet and its effects on the lipid and fatty acid components of eggs,semen and livers[J].Fish Science,2001,67(5):818-827
    [39]Sargent J R,Tocher D R,Bell J G.The Lipids[M].In:Halver(Eds.),Fish Nutrition(2nd ed).London:Academic Press,2002,181-257
    [40]Tago A,Yamamoto Y,Teshima S,et al.Effects of 1,2-di-20:5-phosphatidylcholine(PC)and 1,2-di-22:6-PCon growth and stress tolerance of Japanese flounder(Paralichthys olivaceus)larvae[J].Aquaculture,1999,179(1-4):231-239
    [41]Linares F,Henderson R J.Incorporation of 14C-labelled polyunsaturated fatty acids by juvenile turbot,Scophthalmus maximus(L.)in vivo[J].Journal of Fish Biology,2010,38(3):335-347

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