甜瓜盐碱逆境生理响应及相关基因研究进展
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摘要
综述了近年来甜瓜(Cucumis melo L.)在盐碱逆境胁迫下的生理指标变化规律以及相关基因的研究进展,主要包括渗透调节类物质及相关基因、逆境胁迫蛋白、转录因子等,为甜瓜抗逆性研究和生产实践提供参考。
The paper reviews recent-year advances of researching into the physiological index variations of melon(Cucumis melo L.) under saline stress and their related genes,mainly including osmoregulatory substances and related genes,stress proteins,transcription factors,and so on,so as to provide clues for research on melon's stress resistance and melon production.
The paper reviews recent-year advances of researching into the physiological index variations of melon(Cucumis melo L.) under saline stress and their related genes,mainly including osmoregulatory substances and related genes,stress proteins,transcription factors,and so on,so as to provide clues for research on melon's stress resistance and melon production.
引文
[1]PIERIK R.TESTERINK C.The art of being flexible:How to escape from shade,salt,and drought[J].Plant Physiol,2014,166(1):5-22.
[2]农业部.2013年全国各地蔬菜、西瓜、甜瓜、草莓、马铃薯播种面积和产量[J].中国蔬菜,2015(1):12.
[3]MAHAJAN S.TUTEJA N.Cold,salinity and drought stresses:an overview[J].Arch Biochem Biophys,2005,444(2):139-158.
[4]张玉鑫,刘芳,康恩祥,等.rqaCl胁迫下甜瓜幼苗离子吸收特性研究[J].植物营养与肥料学报,2008,14(3):533-539.
[5]SrVRITEPE H O.SIVRITEPE N,ERI M A,et al.The effects of NaCl pre-treatments on salt tolerance of melons grown under long-term salinity[J].Sci Horti,2005,106(4):568-581.
[6]KAYA C.TUNA A L.ASHRAF M,et al.Improved salt tolerance of melon(Cucumis melo L.)by the addition of proline and potassium nitrate[J].Environ Exp Bot,2007,60(3):397-403.
[7]BORDAS M.MONTESINOS C.DABAUZA M.et al.Transfer of the yeast salt tolerance gene HAL1 to Cucumis melo L.cultivars and in vitro evaluation of salt tolerance[J].Transgenic Res,1997,6(1):41-50.
[8]刘景安,孙玉文,祁家保,等.持续亚低温对甜瓜幼苗生理生化指标的影响[J].中国农学通报,2008,24(11):240-243.
[9]张玉鑫,康恩祥,马凌之,等.NaCl胁迫对甜瓜幼苗叶片膜脂过氧化和渗透调节物质的影响[J].果树学报,2007,24(2):194-198.
[10]陈嘉贝.盐胁迫下两个甜瓜品种的转录组分析[D].上海:上海交通大学,2014.
[11]HONG Z,LAKKINENI K,ZHANG Z.et al.Removal of feedback inhibition of Al-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress[J].Plant physiol,2000,122(4):1129-1136.
[12]ASHRAF M.FOOLAD M.Roles of glycine betaine and proline in improving plant abiotic stress resistance[J].Environ Exp Bot,2007,59(2):206-216.
[13]SAKAMOTO A,MURATA N.Genetic engineering of glycinebetaine synthesis in plants:current status and implications for enhancement of stress tolerance[J].J Exp Bot,2000,51(342):81-88.
[14]丛红滋,于喜艳,王秀峰,等.甜瓜中甜菜碱醛脱氢酶基因CmBADH的克隆及非生物胁迫下的表达分析[J].园艺学报,2013,40(5):905-912.
[15]RIMANDO A M,PERKINS-VEAZIE P M.Determination of citrulline in watermelon rind.[J].Journal of Chromatography A,2005,1078(1-2):196-200.
[16]KUSVURAN S.DASGAN H Y.ABAK K.Citrulline is an important biochemical indicator in tolerance to saline and drought stresses in melon[J].The Scientific World J,2013,2013(5):1482-1485.
[17]MUNNS R.Genes and salt tolerance bringing them together[J].New phytol,2005,167(3):645-663.
[18]马光恕,廉华,靳亚忠,等.海藻糖对NaCl胁迫下甜瓜幼苗生长的影响[J].广东农业科学,2013,40(16):4043.
[19]马兵钢,施江,肖璐.甜瓜叶片的衰老与超氧化物歧化酶活性及脂质过氧化作用的关系[J].石河子大学学报(自然科学版),2005,4(1):26-32.
[20]KUSVURAN S,ELLIALTIOGLU S,YASAR F,et al.Antioxidative enzyme activities in the leaves and callus tissues of salt-tolerant and saltsusceptible melon varieties under salinity[J].Afr J Biotechnol,2014,11(3):635-641.
[21]DEINLEIN U,STEPHAN A B.HORTE T,et al.Plant salt-tolerance mechanisms[J].Trends Plant Sci,2014,19(6):371-379.
[22]ZHANG Y D,VERY A A,WANG L M,et al.A K~+channel from salt-tolerant melon inhibited by Na~+[J].New Phytol,2011,189(3):856-868.
[23]张屹东,黄丹枫,牛庆良,等.K~+通道基因MIRK在网纹甜瓜植株中的表达分析[J].华北农学报,2007,22(4):46-51.
[24]谢亚丽.甜瓜钾离子通道基因MIRK的拟南芥转化及转基因植株的耐盐性研究[D].上海:上海交通大学,2009.
[25]王黎敏,张屹东,邓扬悟,等.甜瓜钾离子通道MIRK受Na~+抑制的分子机理[J].植物生理学报,2011,47(2):193-198.
[26]WANG L M,WEI S W,CHEN J B,et al.Regulation of the inward rectifying K~+channel MIRK and ion distribution in two melon cultivars(Cucumis melo L.)under NaCl salinity stress[J].Acta Physiol Plant,2013,35(9):2789-2800.
[27]王澍,朱春燕,叶俊,等.Na~+/H~+逆向转运蛋白基因在甜瓜不同品种中的表达[J].上海交通大学学报(农业科学版),2011,29(5):49-54.
[28]WANG S,ZHANG Y D,PEREZ P G,et al.Isolation and characterization of a vacuolar Na~+/H~+antiporter gene from Cucumis melo L.[J].Afr J Biotech,2011,10(10):1752-1759.
[29]SMART L B.MOSKAL W A,CAMERON K D,et al.MIP genes are down-regulated under drought stress in Nicotiana glauca[J].Plant Cell Physiol,2001,42(7):686-693.
[30]WAN X,MO A,LIU S,et al.Constitutive expression of a peanut ubiquitin-conjugating enzyme gene in Arabidopsis confers improved water-stress tolerance through regulation of stress-responsive gene expression[J].J Biosci Bioeng,2011,111(4):478-484.
[31]ZHOU G A,CHANG R Z,QIU L J.Overexpression of soybean ubiquitin-conjugating enzyme gene GmUBC2 confers enhanced drought and salt tolerance through modulating abiotic stress-responsive gene expression in Arabidopsis[J].Plant Mol Biol,2010,72(4-5):357-367.
[32]YAN J,WANG J,LI Q,et al.AtCHIP,a U-box-containing E3 ubiquitin ligase,plays a critical role in temperature stress tolerance in Arabidopsis[J].Plant Physiol,2003,132(2):861-869.
[33]BALOGLU M C.PATIR M G.Molecular characterization,3D model analysis,and expression pattern of the CmUBC gene encoding the melon ubiquitin-conjugating enzyme under drought and salt stress conditions[J].Biochem Genet,2014,52(1-2):90-105.
[34]PEREZ-SALAMO I,PAPDI C,RIGO G,et al.The heat shock factor a4a confers salt tolerance and is regulated by oxidative stress and the mitogen-activated protein kinases MPK3 and MPK6[J].Plant Physiol,2014,165(1):319-334.
[35]SONG N H,AHN Y J.DcHsp17.7,a small heat shock protein in carrot,is tissue-specifically expressed under salt stress and confers tolerance to salinity[J].New Biotechnol,2011,28(6):698-704.
[36]OGAWA D,YAMAGUCHI K,NISHIUCHI T.High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth[J].J Exp Bot,2007,58(12):3373-3383.
[37]WEI S W.WANG L M,ZHANG Y D,et al.Identification of early response genes to salt stress in roots of melon(Cucumis melo L.)seedlings[J].Mol Biol Rep,2013,40(4):2915-2926.
[38]JEONG J S.KIM Y S.BAEK K H,et al.Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions[J].Plant Physiol,2010,153(1):185-197.
[39]ZHENG X,CHEN B,LU G,et al.Overexpression of a NAC transcription factor enhances rice drought and salt tolerance[J].Biochem Bioph Res Co,2009,379(4):985-989.
[40]ASLAM M.GROVER A,SINHA V B,et al.Isolation and characterization of cold responsive NAC gene from Lepidium latifolium[J].Mol Biol Rep,2012,39(10):9629-9638.
[41]WEI S W,ZHANG F R,ZHANG Y D,et al.Comparative analysis of gene expression in two muskmelon cultivars(Cucumis melo L.)under salt stress[J].J Integra Agr,2014,13(10):2132-2140.
[2]农业部.2013年全国各地蔬菜、西瓜、甜瓜、草莓、马铃薯播种面积和产量[J].中国蔬菜,2015(1):12.
[3]MAHAJAN S.TUTEJA N.Cold,salinity and drought stresses:an overview[J].Arch Biochem Biophys,2005,444(2):139-158.
[4]张玉鑫,刘芳,康恩祥,等.rqaCl胁迫下甜瓜幼苗离子吸收特性研究[J].植物营养与肥料学报,2008,14(3):533-539.
[5]SrVRITEPE H O.SIVRITEPE N,ERI M A,et al.The effects of NaCl pre-treatments on salt tolerance of melons grown under long-term salinity[J].Sci Horti,2005,106(4):568-581.
[6]KAYA C.TUNA A L.ASHRAF M,et al.Improved salt tolerance of melon(Cucumis melo L.)by the addition of proline and potassium nitrate[J].Environ Exp Bot,2007,60(3):397-403.
[7]BORDAS M.MONTESINOS C.DABAUZA M.et al.Transfer of the yeast salt tolerance gene HAL1 to Cucumis melo L.cultivars and in vitro evaluation of salt tolerance[J].Transgenic Res,1997,6(1):41-50.
[8]刘景安,孙玉文,祁家保,等.持续亚低温对甜瓜幼苗生理生化指标的影响[J].中国农学通报,2008,24(11):240-243.
[9]张玉鑫,康恩祥,马凌之,等.NaCl胁迫对甜瓜幼苗叶片膜脂过氧化和渗透调节物质的影响[J].果树学报,2007,24(2):194-198.
[10]陈嘉贝.盐胁迫下两个甜瓜品种的转录组分析[D].上海:上海交通大学,2014.
[11]HONG Z,LAKKINENI K,ZHANG Z.et al.Removal of feedback inhibition of Al-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress[J].Plant physiol,2000,122(4):1129-1136.
[12]ASHRAF M.FOOLAD M.Roles of glycine betaine and proline in improving plant abiotic stress resistance[J].Environ Exp Bot,2007,59(2):206-216.
[13]SAKAMOTO A,MURATA N.Genetic engineering of glycinebetaine synthesis in plants:current status and implications for enhancement of stress tolerance[J].J Exp Bot,2000,51(342):81-88.
[14]丛红滋,于喜艳,王秀峰,等.甜瓜中甜菜碱醛脱氢酶基因CmBADH的克隆及非生物胁迫下的表达分析[J].园艺学报,2013,40(5):905-912.
[15]RIMANDO A M,PERKINS-VEAZIE P M.Determination of citrulline in watermelon rind.[J].Journal of Chromatography A,2005,1078(1-2):196-200.
[16]KUSVURAN S.DASGAN H Y.ABAK K.Citrulline is an important biochemical indicator in tolerance to saline and drought stresses in melon[J].The Scientific World J,2013,2013(5):1482-1485.
[17]MUNNS R.Genes and salt tolerance bringing them together[J].New phytol,2005,167(3):645-663.
[18]马光恕,廉华,靳亚忠,等.海藻糖对NaCl胁迫下甜瓜幼苗生长的影响[J].广东农业科学,2013,40(16):4043.
[19]马兵钢,施江,肖璐.甜瓜叶片的衰老与超氧化物歧化酶活性及脂质过氧化作用的关系[J].石河子大学学报(自然科学版),2005,4(1):26-32.
[20]KUSVURAN S,ELLIALTIOGLU S,YASAR F,et al.Antioxidative enzyme activities in the leaves and callus tissues of salt-tolerant and saltsusceptible melon varieties under salinity[J].Afr J Biotechnol,2014,11(3):635-641.
[21]DEINLEIN U,STEPHAN A B.HORTE T,et al.Plant salt-tolerance mechanisms[J].Trends Plant Sci,2014,19(6):371-379.
[22]ZHANG Y D,VERY A A,WANG L M,et al.A K~+channel from salt-tolerant melon inhibited by Na~+[J].New Phytol,2011,189(3):856-868.
[23]张屹东,黄丹枫,牛庆良,等.K~+通道基因MIRK在网纹甜瓜植株中的表达分析[J].华北农学报,2007,22(4):46-51.
[24]谢亚丽.甜瓜钾离子通道基因MIRK的拟南芥转化及转基因植株的耐盐性研究[D].上海:上海交通大学,2009.
[25]王黎敏,张屹东,邓扬悟,等.甜瓜钾离子通道MIRK受Na~+抑制的分子机理[J].植物生理学报,2011,47(2):193-198.
[26]WANG L M,WEI S W,CHEN J B,et al.Regulation of the inward rectifying K~+channel MIRK and ion distribution in two melon cultivars(Cucumis melo L.)under NaCl salinity stress[J].Acta Physiol Plant,2013,35(9):2789-2800.
[27]王澍,朱春燕,叶俊,等.Na~+/H~+逆向转运蛋白基因在甜瓜不同品种中的表达[J].上海交通大学学报(农业科学版),2011,29(5):49-54.
[28]WANG S,ZHANG Y D,PEREZ P G,et al.Isolation and characterization of a vacuolar Na~+/H~+antiporter gene from Cucumis melo L.[J].Afr J Biotech,2011,10(10):1752-1759.
[29]SMART L B.MOSKAL W A,CAMERON K D,et al.MIP genes are down-regulated under drought stress in Nicotiana glauca[J].Plant Cell Physiol,2001,42(7):686-693.
[30]WAN X,MO A,LIU S,et al.Constitutive expression of a peanut ubiquitin-conjugating enzyme gene in Arabidopsis confers improved water-stress tolerance through regulation of stress-responsive gene expression[J].J Biosci Bioeng,2011,111(4):478-484.
[31]ZHOU G A,CHANG R Z,QIU L J.Overexpression of soybean ubiquitin-conjugating enzyme gene GmUBC2 confers enhanced drought and salt tolerance through modulating abiotic stress-responsive gene expression in Arabidopsis[J].Plant Mol Biol,2010,72(4-5):357-367.
[32]YAN J,WANG J,LI Q,et al.AtCHIP,a U-box-containing E3 ubiquitin ligase,plays a critical role in temperature stress tolerance in Arabidopsis[J].Plant Physiol,2003,132(2):861-869.
[33]BALOGLU M C.PATIR M G.Molecular characterization,3D model analysis,and expression pattern of the CmUBC gene encoding the melon ubiquitin-conjugating enzyme under drought and salt stress conditions[J].Biochem Genet,2014,52(1-2):90-105.
[34]PEREZ-SALAMO I,PAPDI C,RIGO G,et al.The heat shock factor a4a confers salt tolerance and is regulated by oxidative stress and the mitogen-activated protein kinases MPK3 and MPK6[J].Plant Physiol,2014,165(1):319-334.
[35]SONG N H,AHN Y J.DcHsp17.7,a small heat shock protein in carrot,is tissue-specifically expressed under salt stress and confers tolerance to salinity[J].New Biotechnol,2011,28(6):698-704.
[36]OGAWA D,YAMAGUCHI K,NISHIUCHI T.High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth[J].J Exp Bot,2007,58(12):3373-3383.
[37]WEI S W.WANG L M,ZHANG Y D,et al.Identification of early response genes to salt stress in roots of melon(Cucumis melo L.)seedlings[J].Mol Biol Rep,2013,40(4):2915-2926.
[38]JEONG J S.KIM Y S.BAEK K H,et al.Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions[J].Plant Physiol,2010,153(1):185-197.
[39]ZHENG X,CHEN B,LU G,et al.Overexpression of a NAC transcription factor enhances rice drought and salt tolerance[J].Biochem Bioph Res Co,2009,379(4):985-989.
[40]ASLAM M.GROVER A,SINHA V B,et al.Isolation and characterization of cold responsive NAC gene from Lepidium latifolium[J].Mol Biol Rep,2012,39(10):9629-9638.
[41]WEI S W,ZHANG F R,ZHANG Y D,et al.Comparative analysis of gene expression in two muskmelon cultivars(Cucumis melo L.)under salt stress[J].J Integra Agr,2014,13(10):2132-2140.