Poly(I∶C)增强口蹄疫病毒样颗粒疫苗免疫效果的研究
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摘要
口蹄疫是一种高度接触性传染病,严重影响着畜牧业的健康发展。目前主要通过接种疫苗对其进行预防。近年来,病毒样颗粒(virus-like partic,lVeLsPs)疫苗由于具有天然病毒相似的结构、不含有病毒的遗传物质、易被免疫细胞识别等优点,逐渐成为研究的热点。为了进一步提高口蹄疫VLPs疫苗的免疫效果,本研究选取poly(I∶C)联合ISA 201共同使用,以探究poly(I∶C)对口蹄疫VLPs疫苗免疫效果的影响。结果,poly(I∶C)可以刺激机体产生更高水平的特异性抗体和中和抗体以及IF N-γ、T NF-α和IL-4等细胞因子的分泌,从而引起机体产生更强的体液免疫应答和细胞免疫应答,进而保护动物机体免受口蹄疫病毒的攻击。
Foot-and-mouth disease(FMD) is a highly contagious infectious disease that seriously affects the development of animal husbandry.It is currently mainly prevented by vaccination.In recent years,virus-like particles(VLPs) vaccines have become a research hotspot because of their similar structures to natural viruses, not containing genetic materials of the viruses,and easy identification by immune cells.In order to further improve the immune effect of foot-and-mouth VLPs vaccines,this study selected poly(Ⅰ∶ C) combined with ISA 201 to explore their immune effect on foot-and-mouth disease VLPs vaccine.The results showed that poly(Ⅰ∶ C) can stimulate swine to produce higher level of FMD virus(FMDV) specific antibodies and neutralizing antibodies,and promote the secretion of cytokines such as IFN-γ,TNF-α and IL-4,thereby causing humoral and cellular immune responses,and making the swine get better protection from FMDV challenge.
Foot-and-mouth disease(FMD) is a highly contagious infectious disease that seriously affects the development of animal husbandry.It is currently mainly prevented by vaccination.In recent years,virus-like particles(VLPs) vaccines have become a research hotspot because of their similar structures to natural viruses, not containing genetic materials of the viruses,and easy identification by immune cells.In order to further improve the immune effect of foot-and-mouth VLPs vaccines,this study selected poly(Ⅰ∶ C) combined with ISA 201 to explore their immune effect on foot-and-mouth disease VLPs vaccine.The results showed that poly(Ⅰ∶ C) can stimulate swine to produce higher level of FMD virus(FMDV) specific antibodies and neutralizing antibodies,and promote the secretion of cytokines such as IFN-γ,TNF-α and IL-4,thereby causing humoral and cellular immune responses,and making the swine get better protection from FMDV challenge.
引文
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[13]周春雪,魏巍,李冬,等.Al(OH)3、ISA206及Poly(I∶C)免疫佐剂对灭活口蹄疫140S抗原免疫效果的影响[J].免疫学杂志,2013,11:927-932.ZHOU Chun-xue,WEI Wei,LI Dong,et al.Comparative study for the immune efficacy of Al(OH)3,ISA 206and poly(I∶C)as inactivated FMDV 140S adjuvants.[J].Immunological Journal,2013,11:927-932.(in Chinese).
[14]MA M,JIN N,SHEN G,et al.Immune responses of swine inoculated with a recombinant fowlpox virus coexpressing P12A and 3C of FMDV and swine IL-18[J].Veterinary Immunology and Immunopathology,2008,121(1-2):1-7.
[15]TEWARI K,FLYNN B J,BOSCARDIN S B,et al.Poly(I∶C)is an effective adjuvant for antibody and multi-functional CD4+T cell responses to Plasmodium falciparum circumsporozoite protein(CSP)and alphaDEC-CSP in non human primates[J].Vaccine,2010,28(45):7256-7266.
[16]CAO Y,LU Z,LI P,et al.Improved neutralising antibody response against foot-and-mouth disease virus in mice inoculated with a multi-epitope peptidevaccineusingpolyinosinicandpoly-cytidylic acid as an adjuvant[J].Journal of Virological Methods,2012,185(1):124-128.
[17]CAO Y,LU Z,LI Y,et al.Poly(I:C)combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs[J].Antiviral Research,2013,97(2):145-153.
[2]VAN MAANEN C.A complex-trapping-blocking(CTB)ELISA,using monoclonal antibodies and detecting specifically antibodies directed against footand-mouth disease types A,O and C.Ⅱ.Application[J].Veterinary Microbiology,1990,24(2):179-191.
[3]曹佳媛,田明尧,辛舒,等.以乙型肝炎病毒核心蛋白为载体的PRRSV病毒样颗粒疫苗的构建[J].中国兽医科学,2015,45(10):1000-1004.CAO Jia-yuan,TIAN Ming-yao,XIN Shu,et al.Construction of American serotype porcine reproductive and respiratory syndrome virus virus-like particles vaccine using hepatitis B virus core protein as a vector[J].Chinese Veterinary Science,2015,45(10):1000-1004.
[4]TAN M,JIANG X.Subviral particle as vaccine and vaccine platform[J].Current Opinion in Virology,2014,6:24-33.
[5]JAIN N K,SAHNI N,KUMRU O S,et al.Formulation and stabilization of recombinant protein based viruslike particle vaccines[J].Advanced Drug Delivery Reviews,2015,93:42-55.
[6]朱向涛,孙世琪,吴颉,等.壳聚糖季铵盐凝乳胶微球对O型口蹄疫VLPs免疫豚鼠的免疫增强作用[J].中国兽医科学,2017,47(10):1240-1244.ZHU Xiang-tao,SUN Shi-qi,WU Jie,et al.Immunological enhancement of the quaternized chitosan-gelmicrospheres on immunization of virus-like particles offoot-and-mouth disease virus serotype O in guinea pigs[J].Chinese Veterinary Science,2017,47(10):1240-1244.(in Chinese)
[7]REED S G,ORR M T,FOX C B.Key roles of adjuvants in modern vaccines[J].Nature Medicine,2013,19(12):1597-1608.
[8]COFFMAN R L,SHER A,SEDER R A.Vaccine adjuvants:putting innate immunity to work[J].Immunity,2010,33(4):492-503.
[9]DE GREGORIO E,CAPRONI E,ULMER J B.Vaccine adjuvants:mode of action[J].Frontiers in Immunology,2013,4:214.
[10]ICHINOHE T,AINAI A,TASHIRO M,et al.PolyI:polyC12U adjuvant-combined intranasal vaccine protects mice against highly pathogenic H5N1 influenza virus variants[J].Vaccine,2009,27(45):6276-6279.
[11]ZHU Q,EGELSTON C,GAGNON S,et al.Using 3 TLR ligands as a combination adjuvant induces qualitative changes in T cell responses needed for antiviral protection in mice[J].Journal of Clinical Investigation,2010,120(2):607-616.
[12]王丹阳,杨雨,杨秀梅,等.PolyI:C协同IL-15作为小鼠卵透明带3基因疫苗佐剂黏膜免疫效果的观察[J].中国生物工程杂志,2015,9:7-13.WANG Dan-yang,YANG Yu,YANG Xiu-mei,et al.The efficacy of poly I∶C and IL-15 as adjuvant the murine zona pellucid 3DNA vaccine intranasally immunized.[J].China Biotechnology,2015,9:7-13.(in Chinese)
[13]周春雪,魏巍,李冬,等.Al(OH)3、ISA206及Poly(I∶C)免疫佐剂对灭活口蹄疫140S抗原免疫效果的影响[J].免疫学杂志,2013,11:927-932.ZHOU Chun-xue,WEI Wei,LI Dong,et al.Comparative study for the immune efficacy of Al(OH)3,ISA 206and poly(I∶C)as inactivated FMDV 140S adjuvants.[J].Immunological Journal,2013,11:927-932.(in Chinese).
[14]MA M,JIN N,SHEN G,et al.Immune responses of swine inoculated with a recombinant fowlpox virus coexpressing P12A and 3C of FMDV and swine IL-18[J].Veterinary Immunology and Immunopathology,2008,121(1-2):1-7.
[15]TEWARI K,FLYNN B J,BOSCARDIN S B,et al.Poly(I∶C)is an effective adjuvant for antibody and multi-functional CD4+T cell responses to Plasmodium falciparum circumsporozoite protein(CSP)and alphaDEC-CSP in non human primates[J].Vaccine,2010,28(45):7256-7266.
[16]CAO Y,LU Z,LI P,et al.Improved neutralising antibody response against foot-and-mouth disease virus in mice inoculated with a multi-epitope peptidevaccineusingpolyinosinicandpoly-cytidylic acid as an adjuvant[J].Journal of Virological Methods,2012,185(1):124-128.
[17]CAO Y,LU Z,LI Y,et al.Poly(I:C)combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs[J].Antiviral Research,2013,97(2):145-153.