健康成人自发染色体畸变率与微核率的本底状况再分析
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摘要
目的:了解环境因素对人类遗传物质的影响,以便正确评估放射从业人员以及低剂量辐射的健康效应。方法:选自北京市87名接受健康体检的成年人,每人用肝素钠抗凝管抽取外周静脉血2 mL。采用提前加秋水仙素和松胞素B法分别制备染色体与微核标本,Metafer自动分析系统结合人工分析染色体畸变与双核淋巴细胞微核,按不同年龄段与不同性别分组进行统计比较。结果:87名受检者的染色单体型畸变率平均为0.70%,染色体型畸变率为0.20%,其中双着丝粒体+环为0.13%,单纯无着丝粒体为0.06%。微核与微核细胞率分别为25.30‰和21.56‰。除单体型畸变外,染色体型畸变率与微核率均随年龄的增加而增加(P<0.05),不同性别之间差异无统计学意义(>0.05)。结论:健康成人染色体型畸变(双着丝粒体+环)率与双核淋巴细胞微核率未超过国内外报道的本底范围,但较国内以往报道的本底值有增高趋势。
OBJECTIVE: In order to understand genetic effect from environment factors and to assess correctly the health effect of low dose radiation on occupational personnel. METHODS:The investigated objects came from 87 healthy adults who took routine physical examination and living in Beijing city. 2 mL peripheral venous blood from each person was drawn into heparinized tubes. Conventional chromosome culture with adding colchicines at the beginning and Cyt-B micronuclei(MN) methods were applied. Chromosomal aberrations(CA) and binucleated lymphocyte MN were analyzed using Metafer scanning system combined with manual work. Comparisons according to different age groups and both genders were carried out. RESULTS:The average spontaneous frequencies of chromatid-type and chromosomaltype aberrations in 87 tested persons were 0.70% and 0.20%,respectively. In chromosomal-type aberrations, the dicentrics plus rings(dic+r) were 0.13%,acentrics alone were 0.06%. Spontaneous MN and MNC frequencies were 23.43‰ and 20.47‰,respectively. Except chromatid-type aberration,spontaneous frequencies of CA and MN increased with age(P<0.05) and there were no distinct differences between genders(P>0.05). CONCLUSION: The spontaneous frequencies of chromosomal aberration(dic+r) and micronucleus did not exceed international background range,but showed a rising trend when compared with former reports in China.
OBJECTIVE: In order to understand genetic effect from environment factors and to assess correctly the health effect of low dose radiation on occupational personnel. METHODS:The investigated objects came from 87 healthy adults who took routine physical examination and living in Beijing city. 2 mL peripheral venous blood from each person was drawn into heparinized tubes. Conventional chromosome culture with adding colchicines at the beginning and Cyt-B micronuclei(MN) methods were applied. Chromosomal aberrations(CA) and binucleated lymphocyte MN were analyzed using Metafer scanning system combined with manual work. Comparisons according to different age groups and both genders were carried out. RESULTS:The average spontaneous frequencies of chromatid-type and chromosomaltype aberrations in 87 tested persons were 0.70% and 0.20%,respectively. In chromosomal-type aberrations, the dicentrics plus rings(dic+r) were 0.13%,acentrics alone were 0.06%. Spontaneous MN and MNC frequencies were 23.43‰ and 20.47‰,respectively. Except chromatid-type aberration,spontaneous frequencies of CA and MN increased with age(P<0.05) and there were no distinct differences between genders(P>0.05). CONCLUSION: The spontaneous frequencies of chromosomal aberration(dic+r) and micronucleus did not exceed international background range,but showed a rising trend when compared with former reports in China.
引文
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[3]白玉书,王知权,唐卫生,等.对影响胞质分裂阻断微核自发率因素的探讨[J].中国辐射卫生,1992,1(2):81-82.
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[7]Fenech M,Morley AA.cytokinesis-block micronucleus method in human lymphocytes:Effect of in vivo ageing and low-dose X-irradiation[J].Mutat Res,1986,161(2):193-198.
[8]Fenech M,Neville S,Rinaldi J.Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesis blocked lymphocytes[J].Mutat Res,1994,313(2/3):203-207.
[9]Joksic G,Petrovic S,Ilic Z.Age-related changes in radiationinduced micronuclei among healthy adults[J].Braz J Med&Biol Res,2004,37(8):1111-1117.
[10]Jones K H,York T P,Juusola J,et a l.G enetic a nd e nvironmental influences on spontaneous micronuclei frequencies in children and adults:a t win s tudy[J].M utagenesis,2011,26(6):745-752.
[11]白玉书,陈德清.人类辐射细胞遗传学[M].北京:人民卫生出版社,2006:111-112.
[12]International Atomic Energy Agency.Cytogenetic dosimetry:Applications in preparedness for and response to radiation emergencies[S].Vienna:IAEA,2011:117.
[2]白玉书,关树荣,张秀霞.人淋巴细胞染色体畸变自发率及其与性别年龄关系的研究[J].癌变·畸变·突变,1994,6(1):37-40.
[3]白玉书,王知权,唐卫生,等.对影响胞质分裂阻断微核自发率因素的探讨[J].中国辐射卫生,1992,1(2):81-82.
[4]International Atomic Energy Agency.Technical report series No.405.Cytogenetic analysis for radiation dose assessment[S].Veinna:IAEA,2001:82-83.
[5]王继先,金璀珍,白玉书,等.放射生物剂量学[M].北京:原子能出版社,1997:26.
[6]曹淑媛,周振英,李云华,等.93例正常人的染色体分析[J].核防护,1978,3(1):1-6.
[7]Fenech M,Morley AA.cytokinesis-block micronucleus method in human lymphocytes:Effect of in vivo ageing and low-dose X-irradiation[J].Mutat Res,1986,161(2):193-198.
[8]Fenech M,Neville S,Rinaldi J.Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesis blocked lymphocytes[J].Mutat Res,1994,313(2/3):203-207.
[9]Joksic G,Petrovic S,Ilic Z.Age-related changes in radiationinduced micronuclei among healthy adults[J].Braz J Med&Biol Res,2004,37(8):1111-1117.
[10]Jones K H,York T P,Juusola J,et a l.G enetic a nd e nvironmental influences on spontaneous micronuclei frequencies in children and adults:a t win s tudy[J].M utagenesis,2011,26(6):745-752.
[11]白玉书,陈德清.人类辐射细胞遗传学[M].北京:人民卫生出版社,2006:111-112.
[12]International Atomic Energy Agency.Cytogenetic dosimetry:Applications in preparedness for and response to radiation emergencies[S].Vienna:IAEA,2011:117.