睡莲花多糖的超声波提取工艺及其抗氧化活性研究
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摘要
本研究旨在优选睡莲花多糖(NCP)的超声波提取工艺,并对其体外抗氧化活性进行研究。以多糖得率为指标,采用正交试验设计优选NCP的超声波提取工艺,对所制备的NCP进行分级醇沉,测定NCP对超氧阴离子(O_2~-)、羟基自由基(OH)和1,1-二苯基-2-苦肼基(DPPH)的清除率,以评价其体外抗氧化活性,并与热水浸提法和超声波常温提取法进行对比分析。研究结果表明,NCP的最佳提取工艺条件为料液比1∶25(g/mL)、超声波功率200W、提取温度60℃、提取时间30min,该工艺条件下,NCP得率为(2.08±0.04)%。NCP具有一定的抗氧化活性,以最优工艺组合所制备的NCP抗氧化效果最佳,当多糖浓度为1.0mg/mL时,NCP对DPPH、O_2~-和OH清除率可达50.25%、56.17%和65.48%。分级醇沉及抗氧化活性对比分析结果表明,NCP主要成分为大分子多糖,且NCP的抗氧化活性主要由大分子多糖决定,最优提取工艺条件下所制备的NCP的体外抗氧化活性优于其他提取组合。超声波提取法具有快速高效的特点,适用于NCP的提取分离,可获得更好的提取效果和产品品质。
The aim of this study was to optimize the ultrasonic extraction technology of Polysaccharide from Water Lotus(NCP) and to study its antioxidant activity in vitro. With the yield of polysaccharides as the index, orthogonal design was used to optimize the ultrasonic extraction process of NCP. The prepared NCP was subjected to graded alcohol precipitation.The scavenging rates of NCP on superoxide anion(O_2~-), hydroxyl radical(OH) and 1,1-diphenyl-2-picrylhydrazine(DPPH) were determined to evaluate its antioxidant activity in vitro, and compared with hot water extraction and ultrasonic extraction at room temperature analysis. The results showed that the optimum extraction conditions of NCP were as follows:solid-liquid ratio 1∶25(g/mL), ultrasonic power 200 W, extraction temperature 60 ℃, extraction time 30 min. The yield of NCP was(2.08+0.04)%. NCP had certain antioxidant activity. The antioxidant effect of NCP prepared by the optimum process combination was the best. When the concentration of polysaccharide was 1.0 mg/mL, the scavenging rates of DPPH,O_2~-and OH by NCP could reach 50.25%, 56.17% and 65.48%. The results showed that the main components of NCP were macromolecular polysaccharides, and the antioxidant activity of NCP was mainly determined by macromolecular polysaccharides. The antioxidant activity of NCP prepared under the optimum extraction conditions was better than that of other extraction combinations. Ultrasound extraction method has the characteristics of fast and high efficiency, and is suitable for use in industrial production.
The aim of this study was to optimize the ultrasonic extraction technology of Polysaccharide from Water Lotus(NCP) and to study its antioxidant activity in vitro. With the yield of polysaccharides as the index, orthogonal design was used to optimize the ultrasonic extraction process of NCP. The prepared NCP was subjected to graded alcohol precipitation.The scavenging rates of NCP on superoxide anion(O_2~-), hydroxyl radical(OH) and 1,1-diphenyl-2-picrylhydrazine(DPPH) were determined to evaluate its antioxidant activity in vitro, and compared with hot water extraction and ultrasonic extraction at room temperature analysis. The results showed that the optimum extraction conditions of NCP were as follows:solid-liquid ratio 1∶25(g/mL), ultrasonic power 200 W, extraction temperature 60 ℃, extraction time 30 min. The yield of NCP was(2.08+0.04)%. NCP had certain antioxidant activity. The antioxidant effect of NCP prepared by the optimum process combination was the best. When the concentration of polysaccharide was 1.0 mg/mL, the scavenging rates of DPPH,O_2~-and OH by NCP could reach 50.25%, 56.17% and 65.48%. The results showed that the main components of NCP were macromolecular polysaccharides, and the antioxidant activity of NCP was mainly determined by macromolecular polysaccharides. The antioxidant activity of NCP prepared under the optimum extraction conditions was better than that of other extraction combinations. Ultrasound extraction method has the characteristics of fast and high efficiency, and is suitable for use in industrial production.
引文
[1]买吾拉江·阿不都热衣木,李晨阳,李倩,等.高速逆流色谱分离纯化雪白睡莲花中的酚类成分[J].安徽农业科学,2016(21):107-109.
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[8]麦尔旦·吐尔逊麦麦提,刘涛,李晨阳,等.睡莲花isostrictiniin对CCl4致小鼠急性肝损伤防护作用及其体外抗氧化活性研究[J].新疆医科大学学报,2018(3):321-325.
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[10]马俊飞,张石蕾,吕梅霞,等.睡莲花烟花苷对半乳糖胺致小鼠急性肝损伤的保护作用[J].癌变·畸变·突变,2017(5):370-373.
[11]张石蕾,由淑萍,刘涛,等.睡莲花总黄酮对四氯化碳致小鼠急性肝损伤的防治作用[J].天然产物研究与开发,2016(12):165-168.
[12]蔡锦源,熊建文,黄燕芬,等.白及多糖的超声—微波协同提取工艺及其抗氧化活性研究[J].食品工业科技,2016(22):274-278.
[13]蔡锦源,朱炽雄,孙松,等.香菇多糖的微波预处理—超声波提取工艺及其抗氧化活性研究[J].河南工业大学学报(自然科学版),2016(4):84-90.
[14]黄磊.香菇多糖抗氧化及其抗真菌机制初步研究[J].广东农业科学,2013(20):114-116.
[15]朱赟豪,陈哲,何东平,等.超声波辅助提取油茶籽粕中蛋白的工艺研究[J].粮食科技与经济,2014(4):53-56.
[16]蒲灵操,黄群,袁瑶,等.响应面法优化超声波辅助提取瓜蒌籽蛋白工艺研究[J].粮食科技与经济,2013(5):56-60.
[2]金晓红,耿伟,桂红武.睡莲花总黄酮超声提取工艺研究[J].医药导报,2011(11):1487-1489.
[3]买吾拉江·阿不都热衣木,李倩,王亮,等.HPLC法测定睡莲花中烟花苷和紫云英苷的含量[J].中国民族民间医药,2016(20):20-22.
[4]李洁,张雪佳,李柯翱,等.HPLC测定维药睡莲花中没食子酸和烟花苷含量[J].中国中医药信息杂志,2014(8):92-94.
[5]刘瑞凝,王伟,谢伟东,等.雪白睡莲花中黄酮类成分抗氧化及拟缺血对神经细胞作用的研究[J].世界科学技术:中医药现代化,2006(5):33-36.
[6]景赞,曾维才,黄毅娜,等.蓝睡莲多酚类物质抗氧化与降糖作用的研究[J].食品科技,2010(7):237-241.
[7]李倩,李晨阳,詹羽娇,等.睡莲花提取物对酪氨酸酶的抑制作用及其动力学研究[J].日用化学工业,2017(4):219-223.
[8]麦尔旦·吐尔逊麦麦提,刘涛,李晨阳,等.睡莲花isostrictiniin对CCl4致小鼠急性肝损伤防护作用及其体外抗氧化活性研究[J].新疆医科大学学报,2018(3):321-325.
[9]王雪,杨巧丽,赵军,等.睡莲花提取物对小鼠实验性肝损伤的保护作用研究[J].中药材,2015(1):139-142.
[10]马俊飞,张石蕾,吕梅霞,等.睡莲花烟花苷对半乳糖胺致小鼠急性肝损伤的保护作用[J].癌变·畸变·突变,2017(5):370-373.
[11]张石蕾,由淑萍,刘涛,等.睡莲花总黄酮对四氯化碳致小鼠急性肝损伤的防治作用[J].天然产物研究与开发,2016(12):165-168.
[12]蔡锦源,熊建文,黄燕芬,等.白及多糖的超声—微波协同提取工艺及其抗氧化活性研究[J].食品工业科技,2016(22):274-278.
[13]蔡锦源,朱炽雄,孙松,等.香菇多糖的微波预处理—超声波提取工艺及其抗氧化活性研究[J].河南工业大学学报(自然科学版),2016(4):84-90.
[14]黄磊.香菇多糖抗氧化及其抗真菌机制初步研究[J].广东农业科学,2013(20):114-116.
[15]朱赟豪,陈哲,何东平,等.超声波辅助提取油茶籽粕中蛋白的工艺研究[J].粮食科技与经济,2014(4):53-56.
[16]蒲灵操,黄群,袁瑶,等.响应面法优化超声波辅助提取瓜蒌籽蛋白工艺研究[J].粮食科技与经济,2013(5):56-60.