布氏杆菌脂多糖的研究进展
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摘要
布氏杆菌(Brucella)为革兰阴性胞内寄生球杆菌,可以感染牛、绵羊、山羊、猪、犬等哺乳动物和人,对畜牧业和人类健康危害严重。布氏杆菌脂多糖作为细胞膜的主要组成部分,是布氏杆菌最重要的表面抗原和刺激机体先天免疫的靶点,也是目前布氏杆菌致病分子机制研究和布氏杆菌病诊断、防控研究的热点之一。本文对布氏杆菌LPS的化学结构、生物合成及相关基因、脂多糖与机体天然免疫等方面的研究结果进行综述,以期阐释布氏杆菌的生存和致病机制,为布氏杆菌病的诊断和防控提供技术参考。
Brucella spp.is a Gram-negative and intracellular parasitism cocccobacilli,and it can infect mammals like cattle,sheep,goats,swines,dogs and humans.Brucellosis results in worldwide economic losses in animal husbandry and it is at high risks of human health. Lipopolysacharide(LPS),the main component of the outer membrane, is a major surface antigen and virulence factor of Brucella.LPS also serves as one of the primary targets of the innate arm of the mammalian immune system. LPS is the hottest spots of the studies about molecular pathogenesis, diagnosis, prevention and control of brucellosis.To explain the survival and pathogenesis of Brucella and to provide a technical reference for the diagnosis and prevention of brucellosis,we review the chemical structure and biosynthesis of Brucella LPS and the relationship between LPS and innate immunity.
Brucella spp.is a Gram-negative and intracellular parasitism cocccobacilli,and it can infect mammals like cattle,sheep,goats,swines,dogs and humans.Brucellosis results in worldwide economic losses in animal husbandry and it is at high risks of human health. Lipopolysacharide(LPS),the main component of the outer membrane, is a major surface antigen and virulence factor of Brucella.LPS also serves as one of the primary targets of the innate arm of the mammalian immune system. LPS is the hottest spots of the studies about molecular pathogenesis, diagnosis, prevention and control of brucellosis.To explain the survival and pathogenesis of Brucella and to provide a technical reference for the diagnosis and prevention of brucellosis,we review the chemical structure and biosynthesis of Brucella LPS and the relationship between LPS and innate immunity.
引文
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[2]宁宜宝.兽用疫苗学[M].北京:中国农业出版社,2008:486-487.NING Yi-bao.Veternary Vaccine[M].Beijing:China Agriculture Press,2008:486-487.(in Chinese)
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[10]FREER E,ROJAS N,WEINTRAUB A,et al.Heterogeneity of Brucella abortus lipopolysaccharides[J].Research in Microbiology,1995,146(7):569.
[11]BUNDLE D R,CHERWONOGRODZKY J W,GIDNEY M A,et al.Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides[J].Infection&Immunity,1989,57(9):2829-2836.
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[16]KE Y,ZHEN Q,LI T,et al.Complete genome sequence of Brucella melitensis 133,an isolate of biovar 1of sequence type 32[J].Journal of Bacteriology,2012,194(24):6932.
[17]DE Y,DONG C,CAO Y,et al.Genome-wide sequence transposon insertion sites and analyze the essential genes of Brucella melitensis[J].Microbial Pathogenesis,2017,112(11):97-102.
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[21]李爽.羊种布鲁氏菌新疆流行株Wbd A、Wbk E基因缺失株的构建及评价[D].石河子:石河子大学,2016.LI Shuang.Construction and evaluation of Wbd A gene and Wbk E gene deficient of Brucella melitensis epidemic strain from Xingjiang[D].Shihezi:Shihezi University,2016.(in Chinese)
[22]GOMES M T,CAMPOS P C,DE ALMEIDA L A,et al.The role of innate immune signals in immunity to Brucella abortus[J].Frontiers in Cellular&Infection Microbiology,2012,2(10):130.
[23]TURVEY S E,BROIDE D H.Innate immunity[J].Journal of Allergy&Clinical Immunology,2010,125(2):S24-S32.
[24]HASHIMOTO D,MILLER J,MERAD M.Dendritic cell and macrophage heterogeneity in vivo[J].Immunity,2011,35(3):323.
[25]WYNN T A,CHAWLA A,POLLARD J W.Macrophage biology in development,homeostasis and disease[J].Nature,2013,496(7446):445-455.
[26]冯宇,丁家波.布鲁氏菌免疫应答研究进展[J].生命科学,2016,28(9):1067-1074.FENG Yu,DING Jia-bo.Research advance on immune response against Brucella[J].Chinese Bulletin of Life Sciences,2016,28(9):1067-1074.(in Chinese)
[27]KIM S,WATARAI M,SUZUKI H,et al.Lipid raft microdomains mediate class A scav enger receptordependent infection of Brucella abortus[J].Microbial Pathogenesis,2004,37(1):11-19.
[28]CELLI J.Surviving inside a macrophage:the many ways of Brucella[J].Research in Microbiology,2006,157(2):93-98.
[29]CELLI J,GORVEL J P.Organelle robbery:Brucella interactions with the endoplasmic reticulum[J].Current Opinion in Microbiology,2004,7(1):93-97.
[30]董炳梅,王金良,唐娜,等.布鲁氏菌的致病机制与细胞免疫机制研究进展[J].中国人兽共患病学报,2012,28(6):635-639.DONG Bing-mei,WANG Jin-liang,TANG Na,et al.Research progress on pathogenic mechanism and cellular immunity of Brucella[J].Chinese Journal of Zoonoses,2012,28(6):635-639.(in Chinese)
[31]KANG P B,AZAD A K,TORRELLES J B,et al.The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabino mannan-mediated phagosome biogenesis[J].Journal of Experimental Medicine,2005,202(7):987-999.
[32]陈贞里,郑源强,石艳春.布氏菌细胞内生存机制的研究进展[J].医学综述,2017,23(2):209-213.CHEN Zhen-li,ZHENG Yuan-qiang,SHI Yan-chun.Research progress in survival mechanism of Brucella in the host cells[J].Medical Recapitulate,2017,23(2):209-213.(in Chinese)
[33]OSMAN A Y,ABDULLAH F F,KADIR A A,et al.Immuno-pathophysiological responses of mouse model to experimental infection with Brucella melitensis and its lipopolysaccharides via intraperitoneal route[J].Microbial Pathogenesis,2016,100(11):17-29.
[34]OSMAN A Y,SAHAREE A A,JESSE F F,et al.Comparative study of immunopathophysiological responses induced by B.melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure[J].Microbial Pathogenesis,2017,110(9):365-374.
[35]潘文,王佳莹,赵明秋,等.布鲁氏菌bp26基因缺失株的构建[J].中国兽医科学,2011,41(3):280-286.PAN Wen,WANG Jia-ying,ZHAO Ming-qiu,et al.Construction of unmarked bp26 gene-deletedstrains of Brucella spp.[J].Chinese Veterinary Science,2011,41(3):280-286.(in Chinese)
[36]杨春华,邱昌庆,曹小安,等.流产布鲁氏菌omp25基因的克隆与原核表达[J].中国兽医科学,2007,37(6):465-468.YANG Chun-hua,QIU Chang-qing,CAO Xiao-an,et al.Cloning and prokaryotic expression of omp25 gene from Brucella abortus[J].Chinese Veterinary Science,2007,37(6):465-468.(in Chinese)
[2]宁宜宝.兽用疫苗学[M].北京:中国农业出版社,2008:486-487.NING Yi-bao.Veternary Vaccine[M].Beijing:China Agriculture Press,2008:486-487.(in Chinese)
[3]PAPPAS G.The changing Brucella ecology:novel reservoirs,new threats[J].International Journal of Antimicrobial Agents,2010,36(Suppl 1):S8-S11.
[4]BOSCHIROLI M L,FOULONGNE V,CALLAGHAN D O.Brucellosis:a worldwide zoonosis[J].Current Opinion in Microbiology,2001,4(1):58-64.
[5]CORBEL M J,ALTON G G,BANAI M,et al.Brucellosis in Humans and Animals[M].Swizerland:World Health Organization Press,2006:66.
[6]PAPPAS G,PAPADIMITRIOU P,AKRITIDIS N,et al.The new global map of human brucellosis[J].Lancet Infectious Diseases,2006,6(2):91.
[7]SELEEM M N,BOYLE S M,SRIRANGANATHAN N.Brucellosis:a re-emerging zoonosis[J].Veterinary Microbiology,2010,140(3-4):392-398.
[8]CARDOSO P G,MACEDO G C,AZEVEDO V,et al.Brucella spp.noncanonical LPS:structure,biosynthesis,and interaction with host immune system[J].Microbial Cell Factories,2006,5(1):13.
[9]MORENO E,STACKEBRANDT E,DORSCH M,et al.Brucella abortus 16S r RNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria[J].Journal of Bacteriology,1990,172(7):3569-3576.
[10]FREER E,ROJAS N,WEINTRAUB A,et al.Heterogeneity of Brucella abortus lipopolysaccharides[J].Research in Microbiology,1995,146(7):569.
[11]BUNDLE D R,CHERWONOGRODZKY J W,GIDNEY M A,et al.Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides[J].Infection&Immunity,1989,57(9):2829-2836.
[12]LAPAQUE N,MORIYON I,MORENO E,et al.Brucella lipopolysaccharide acts as a virulence factor[J].Current Opinion in Microbiology,2005,8(1):60.
[13]梅建军,石慧英,王兴龙.布鲁氏菌表面抗原研究进展[J].动物医学进展,2005,26(10):13-18.MEI Jian-jun,SHI Hui-ying,WANG Xing-long.Progress on surface antigens of Brucella spp.[J].Progress in Veterinary Medicine,2005,26(10):13-18.(in Chinese)
[14]HALLING S M,PETERSON-BURCH B D,BRICKER B J,et al.Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis[J].Journal of Bacteriology,2005,187(8):2715-2726.
[15]DELVECCHIO V G,KAPATRAL V,ELZER P,et al.The genome of Brucella melitensis[J].Veterinary Microbiology,2002,90(1-4):587-592.
[16]KE Y,ZHEN Q,LI T,et al.Complete genome sequence of Brucella melitensis 133,an isolate of biovar 1of sequence type 32[J].Journal of Bacteriology,2012,194(24):6932.
[17]DE Y,DONG C,CAO Y,et al.Genome-wide sequence transposon insertion sites and analyze the essential genes of Brucella melitensis[J].Microbial Pathogenesis,2017,112(11):97-102.
[18]WARETH G,MELZER F,NEUBAUER H.In Brucella:selective pressure may turn some genes on instead of default off position[J].Medical Hypotheses,2017,103(6):29-31.
[19]GONZ魣LEZ D,MIGUEL M D,ALI T,et al.Brucellosis vaccines:assessment of Brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export[J].PLo S One,2008,3(7):e2760.
[20]FONTANA C,CONDE-譧LVAREZ R,ST魡HLE J,et al.Structural studies of lipopolysaccharide-defective mutants from Brucella melitensis identify a aore oligosaccharide critical in virulence[J].Journal of Biological Chemistry,2016,291(14):7727.
[21]李爽.羊种布鲁氏菌新疆流行株Wbd A、Wbk E基因缺失株的构建及评价[D].石河子:石河子大学,2016.LI Shuang.Construction and evaluation of Wbd A gene and Wbk E gene deficient of Brucella melitensis epidemic strain from Xingjiang[D].Shihezi:Shihezi University,2016.(in Chinese)
[22]GOMES M T,CAMPOS P C,DE ALMEIDA L A,et al.The role of innate immune signals in immunity to Brucella abortus[J].Frontiers in Cellular&Infection Microbiology,2012,2(10):130.
[23]TURVEY S E,BROIDE D H.Innate immunity[J].Journal of Allergy&Clinical Immunology,2010,125(2):S24-S32.
[24]HASHIMOTO D,MILLER J,MERAD M.Dendritic cell and macrophage heterogeneity in vivo[J].Immunity,2011,35(3):323.
[25]WYNN T A,CHAWLA A,POLLARD J W.Macrophage biology in development,homeostasis and disease[J].Nature,2013,496(7446):445-455.
[26]冯宇,丁家波.布鲁氏菌免疫应答研究进展[J].生命科学,2016,28(9):1067-1074.FENG Yu,DING Jia-bo.Research advance on immune response against Brucella[J].Chinese Bulletin of Life Sciences,2016,28(9):1067-1074.(in Chinese)
[27]KIM S,WATARAI M,SUZUKI H,et al.Lipid raft microdomains mediate class A scav enger receptordependent infection of Brucella abortus[J].Microbial Pathogenesis,2004,37(1):11-19.
[28]CELLI J.Surviving inside a macrophage:the many ways of Brucella[J].Research in Microbiology,2006,157(2):93-98.
[29]CELLI J,GORVEL J P.Organelle robbery:Brucella interactions with the endoplasmic reticulum[J].Current Opinion in Microbiology,2004,7(1):93-97.
[30]董炳梅,王金良,唐娜,等.布鲁氏菌的致病机制与细胞免疫机制研究进展[J].中国人兽共患病学报,2012,28(6):635-639.DONG Bing-mei,WANG Jin-liang,TANG Na,et al.Research progress on pathogenic mechanism and cellular immunity of Brucella[J].Chinese Journal of Zoonoses,2012,28(6):635-639.(in Chinese)
[31]KANG P B,AZAD A K,TORRELLES J B,et al.The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabino mannan-mediated phagosome biogenesis[J].Journal of Experimental Medicine,2005,202(7):987-999.
[32]陈贞里,郑源强,石艳春.布氏菌细胞内生存机制的研究进展[J].医学综述,2017,23(2):209-213.CHEN Zhen-li,ZHENG Yuan-qiang,SHI Yan-chun.Research progress in survival mechanism of Brucella in the host cells[J].Medical Recapitulate,2017,23(2):209-213.(in Chinese)
[33]OSMAN A Y,ABDULLAH F F,KADIR A A,et al.Immuno-pathophysiological responses of mouse model to experimental infection with Brucella melitensis and its lipopolysaccharides via intraperitoneal route[J].Microbial Pathogenesis,2016,100(11):17-29.
[34]OSMAN A Y,SAHAREE A A,JESSE F F,et al.Comparative study of immunopathophysiological responses induced by B.melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure[J].Microbial Pathogenesis,2017,110(9):365-374.
[35]潘文,王佳莹,赵明秋,等.布鲁氏菌bp26基因缺失株的构建[J].中国兽医科学,2011,41(3):280-286.PAN Wen,WANG Jia-ying,ZHAO Ming-qiu,et al.Construction of unmarked bp26 gene-deletedstrains of Brucella spp.[J].Chinese Veterinary Science,2011,41(3):280-286.(in Chinese)
[36]杨春华,邱昌庆,曹小安,等.流产布鲁氏菌omp25基因的克隆与原核表达[J].中国兽医科学,2007,37(6):465-468.YANG Chun-hua,QIU Chang-qing,CAO Xiao-an,et al.Cloning and prokaryotic expression of omp25 gene from Brucella abortus[J].Chinese Veterinary Science,2007,37(6):465-468.(in Chinese)