纳米硒对硫酸镍致大鼠睾丸损伤的保护作用
|
摘要
目的探讨纳米硒(nano-selenium, Nano-Se)对硫酸镍(NiSO_4)诱导大鼠睾丸损伤的保护作用,为镍生殖毒性的防治提供新思路。方法采用液相激光加工与制备技术获得Nano-Se胶体溶液。将40只健康成年雄性SD大鼠随机分为5组:对照组(生理盐水)、NiSO_4组(5 mg/kg·bw NiSO_4)、低(5 mg/kg·bw NiSO_4+0.5 mg/kg·bw Nano-Se)、中(5 mg/kg·bw NiSO_4+1 mg/kg·bw Nano-Se)和高剂量Nano-Se干预组(5 mg/kg NiSO_4+2 mg/kg Nano-Se)。以腹腔注射NiSO_4、Nano-Se灌胃的方式连续处理大鼠14 d。苏木素-伊红(HE)染色观察各组睾丸组织病理学改变,并测定睾丸组织中丙二醛(MDA)含量及内质网应激相关基因GRP78、GADD153的mRNA表达水平。结果与对照组比较,NiSO_4组睾丸生精小管破坏,各级生精细胞排列紊乱,细胞数量减少;MDA含量升高(P<0.05),GRP78和GADD153 mRNA表达水平上调(P<0.05)。与NiSO_4组比较,各剂量Nano-Se干预组明显减轻了睾丸的病理损伤,并明显降低MDA含量(P<0.05)和GRP78、GADD153 mRNA表达水平(P<0.05)。结论 Nano-Se可以拮抗硫酸镍所致的大鼠睾丸损伤,其机制可能与减少氧化损伤和抑制内质网应激相关基因GRP78和GADD153表达有关。
引文
[1] 齐刚毅,王大川,张叶茂,等.成都某电冶厂工人尿镍含量调查分析 [J].职业卫生与病伤,2000(1):24-25.
[2] Grandjean P,Andersen O,Nielsen GD.Carcinogenicity of occupational nickel exposures:An evaluation of the epidemiological evidence [J].Am J Ind Med,2010,13(2):193-209.
[3] Pandey R,Kumar R,Singh S,et al.Male reproductive effect of nickel sulphate in mice [J].Biometals,1999,12(4):339-346.
[4] Doreswamy K,Shrilatha B,Rajeshkumar T.Nickel-induced oxidative stress in testis of mice:evidence of DNA damage and genotoxic effects [J].J Androl,2004,25(6):996-1003.
[5] Zou L,Su L,Sun Y,et al.Nickel sulfate induced apoptosis via activating ROS-dependent mitochondria and endoplasmic reticulum stress pathways in rat Leydig cells [J].Environ Toxicol,2017,32(7):1918-1926.
[6] Klein EA.Selenium:epidemiology and basic science [J].J Urol,2004,171(2):50-53.
[7] Zhang J,Wang H,Yan X,et al.Comparison of short-term toxicity between Nano-Se and selenite in mice [J].Life Sci,2005,76(10):1099-1109.
[8] Zhang JS,Gao XY,Zhang LD,et al.Biological effects of a nano red elemental selenium [J].Biofactors,2001,15(1):27-38.
[9] Zhang J,Claverie J,Chaker M,et al.Colloidal metal nanoparticles prepared by laser ablation and their applications[J].Chem Phys Chem,2017,18(9):986-1006.
[10] Su L,Deng Y,Zhang Y,et al.Protective effects of grape seed procyanidin extract against nickel sulfate-induced apoptosis and oxidative stress in rat testes [J].Toxicol Mech Methods,2011,21(6):487-494.
[11] Liu L,He Y,Xiao Z,et al.Effects of selenium nanoparticles on reproductive performance of male sprague-dawley rats at supranutritional and nonlethal levels [J].Biol Trace Elem Res,2017,180(1):81-89.
[12] Spears J W,Harvey R W,Samsell L J.Effects of dietary nickel and protein on growth,nitrogen metabolism and tissue concentrations of nickel,iron,zinc,manganese and copper in calves [J].J Nutr,1986,116(10):1873-1882.
[13] Murawska-Cia?owicz E,Bal W,Januszewska L,et al.Oxidative stress level in the testes of mice and rats during nickel intoxication [J].Sci W J,2012,12:1-5.
[14] 孙应彪,王学习,宋援朝,等.氧化应激在硫酸镍致小鼠睾丸损伤中的作用 [J].中华劳动卫生职业病杂志,2006,24(7):425-426.
[15] Wang H,Zhang J,Yu H.Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes:comparison with selenomethionine in mice [J].Free Radic Biol Med,2007,42(10):1524-1533.
[16] Amin KA,Hashem KS,Alshehri FS,et al.Antioxidant and hepatoprotective efficiency of selenium nanoparticles against acetaminophen-induced hepatic damage [J].Biol Trace Elem Res,2017,175(1):136-145.
[17] Bhattacharjee A,Basu A,Biswas J,et al.Nano-Se attenuates cyclophosphamide-induced pulmonary injury through modulation of oxidative stress and DNA damage in Swiss albino mice [J].Mol Cell Biochem,2015,405(1-2):243-256.
[18] Rezvanfar MA,Rezvanfar MA,Shahverdi AR,et al.Protection of cisplatin-induced spermatotoxicity,DNA damage and chromatin abnormality by selenium nano-particles [J].Toxicol Appl Pharmacol,2013,266(3):356-365.
[19] Asri-Rezaei S,Nourian A,Shalizar-Jalali A,et al.Selenium supplementation in the form of selenium nanoparticles and selenite sodium improves mature male mice reproductive performances [J].Iran J Basic Med Sci,2018,21(6):577-585.
[20] Zhang K,Kaufman RJ.From endoplasmic-reticulum stress to the inflammatory response [J].Nature,2008,454(7203):455-462.
[21] 宋小燕,赵永波,周晓琳,等.大鼠脑缺血再灌注后 GRP78 和 GADD153 的表达变化研究 [J].中风与神经疾病杂志,2008,25(2):139-141.
[22] Boot-Handford RP,Briggs MD.The unfolded protein response and its relevance to connective tissue diseases [J].Cell Tissue Res,2010,339(1):197-211.
[23] Oyadomari S,Mori M.Roles of CHOP/GADD153 in endoplasmic reticulum stress [J].Cell Death Differ,2004,11(4):381-389.
[24] Song B,Scheuner D,Ron D,et al.Chop deletion reduces oxidative stress,improves β cell function,and promotes cell survival in multiple mouse models of diabetes [J].J Clin Invest,2008,118(10):3378-3389.
[2] Grandjean P,Andersen O,Nielsen GD.Carcinogenicity of occupational nickel exposures:An evaluation of the epidemiological evidence [J].Am J Ind Med,2010,13(2):193-209.
[3] Pandey R,Kumar R,Singh S,et al.Male reproductive effect of nickel sulphate in mice [J].Biometals,1999,12(4):339-346.
[4] Doreswamy K,Shrilatha B,Rajeshkumar T.Nickel-induced oxidative stress in testis of mice:evidence of DNA damage and genotoxic effects [J].J Androl,2004,25(6):996-1003.
[5] Zou L,Su L,Sun Y,et al.Nickel sulfate induced apoptosis via activating ROS-dependent mitochondria and endoplasmic reticulum stress pathways in rat Leydig cells [J].Environ Toxicol,2017,32(7):1918-1926.
[6] Klein EA.Selenium:epidemiology and basic science [J].J Urol,2004,171(2):50-53.
[7] Zhang J,Wang H,Yan X,et al.Comparison of short-term toxicity between Nano-Se and selenite in mice [J].Life Sci,2005,76(10):1099-1109.
[8] Zhang JS,Gao XY,Zhang LD,et al.Biological effects of a nano red elemental selenium [J].Biofactors,2001,15(1):27-38.
[9] Zhang J,Claverie J,Chaker M,et al.Colloidal metal nanoparticles prepared by laser ablation and their applications[J].Chem Phys Chem,2017,18(9):986-1006.
[10] Su L,Deng Y,Zhang Y,et al.Protective effects of grape seed procyanidin extract against nickel sulfate-induced apoptosis and oxidative stress in rat testes [J].Toxicol Mech Methods,2011,21(6):487-494.
[11] Liu L,He Y,Xiao Z,et al.Effects of selenium nanoparticles on reproductive performance of male sprague-dawley rats at supranutritional and nonlethal levels [J].Biol Trace Elem Res,2017,180(1):81-89.
[12] Spears J W,Harvey R W,Samsell L J.Effects of dietary nickel and protein on growth,nitrogen metabolism and tissue concentrations of nickel,iron,zinc,manganese and copper in calves [J].J Nutr,1986,116(10):1873-1882.
[13] Murawska-Cia?owicz E,Bal W,Januszewska L,et al.Oxidative stress level in the testes of mice and rats during nickel intoxication [J].Sci W J,2012,12:1-5.
[14] 孙应彪,王学习,宋援朝,等.氧化应激在硫酸镍致小鼠睾丸损伤中的作用 [J].中华劳动卫生职业病杂志,2006,24(7):425-426.
[15] Wang H,Zhang J,Yu H.Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes:comparison with selenomethionine in mice [J].Free Radic Biol Med,2007,42(10):1524-1533.
[16] Amin KA,Hashem KS,Alshehri FS,et al.Antioxidant and hepatoprotective efficiency of selenium nanoparticles against acetaminophen-induced hepatic damage [J].Biol Trace Elem Res,2017,175(1):136-145.
[17] Bhattacharjee A,Basu A,Biswas J,et al.Nano-Se attenuates cyclophosphamide-induced pulmonary injury through modulation of oxidative stress and DNA damage in Swiss albino mice [J].Mol Cell Biochem,2015,405(1-2):243-256.
[18] Rezvanfar MA,Rezvanfar MA,Shahverdi AR,et al.Protection of cisplatin-induced spermatotoxicity,DNA damage and chromatin abnormality by selenium nano-particles [J].Toxicol Appl Pharmacol,2013,266(3):356-365.
[19] Asri-Rezaei S,Nourian A,Shalizar-Jalali A,et al.Selenium supplementation in the form of selenium nanoparticles and selenite sodium improves mature male mice reproductive performances [J].Iran J Basic Med Sci,2018,21(6):577-585.
[20] Zhang K,Kaufman RJ.From endoplasmic-reticulum stress to the inflammatory response [J].Nature,2008,454(7203):455-462.
[21] 宋小燕,赵永波,周晓琳,等.大鼠脑缺血再灌注后 GRP78 和 GADD153 的表达变化研究 [J].中风与神经疾病杂志,2008,25(2):139-141.
[22] Boot-Handford RP,Briggs MD.The unfolded protein response and its relevance to connective tissue diseases [J].Cell Tissue Res,2010,339(1):197-211.
[23] Oyadomari S,Mori M.Roles of CHOP/GADD153 in endoplasmic reticulum stress [J].Cell Death Differ,2004,11(4):381-389.
[24] Song B,Scheuner D,Ron D,et al.Chop deletion reduces oxidative stress,improves β cell function,and promotes cell survival in multiple mouse models of diabetes [J].J Clin Invest,2008,118(10):3378-3389.