鸡排中一株凝固酶异常的金黄色葡萄球菌的分离鉴定
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摘要
目的分离鉴定鸡排中一株凝固酶异常的金黄色葡萄球菌。方法采用国标法检测金黄色葡萄球菌,血浆凝固酶试验时间延长至24 h,同时用生化鉴定法和BAX System Q7快速检测法进行互相验证。结果国标法凝固酶试验6 h不凝固, 12 h出现部分凝固, 24 h完全凝固,生化鉴定法和BAX System Q7快速检测法结果都是检出金黄色葡萄球菌。结论在食品中金黄色葡萄球菌检验过程中,对于凝固酶试验异常的情况,应延长试验时间并用其他方法加以验证。
Objective To isolate and identify a strain of Staphylococcus aureus with coagulase abnormality in chicken chops. Methods The national standard method was used to detect Staphylococcus aureus, the plasma coagulase test time was extended to 24 h, and the biochemical identification method and BAX System Q7 rapid detection method were used to verify each other. Results The national standard coagulase test showed no coagulation for 6 h, partial coagulation for 12 h and complete coagulation for 24 h. The results of biochemical identification method and BAX system Q7 rapid detection method were all positive for Staphylococcus aureus. Conclusions In the process of testing Staphylococcus aureus in food, the abnormality of coagulase test should be prolonged and verified by other methods.
Objective To isolate and identify a strain of Staphylococcus aureus with coagulase abnormality in chicken chops. Methods The national standard method was used to detect Staphylococcus aureus, the plasma coagulase test time was extended to 24 h, and the biochemical identification method and BAX System Q7 rapid detection method were used to verify each other. Results The national standard coagulase test showed no coagulation for 6 h, partial coagulation for 12 h and complete coagulation for 24 h. The results of biochemical identification method and BAX system Q7 rapid detection method were all positive for Staphylococcus aureus. Conclusions In the process of testing Staphylococcus aureus in food, the abnormality of coagulase test should be prolonged and verified by other methods.
引文
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[9]容艳芬,李艳嫦,卢勉飞,等.兔血浆纤维蛋白原琼脂培养基技术与Baird-Parker琼脂培养基技术检测食品中金黄色葡萄球菌的比较[J].中国卫生检验杂志,2017,25(7):642-648.Rong YF,Li YC,Lu MF,et al.Comparison of rabbit plasma fibrinogen agar medium and Baird-Parker agar medium for detection of Staphylococcus aureus in food[J].Chin J Health Lab Technol,2017,25(7):642-648.
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[12]李海丽,柯娴,邓金兰,等.沙门氏菌检测方法的优化[J].中外食品工业,2013,(12):23-24.Li HL,Ke X,Deng JL,et al.Optimization of Salmonella detection method[J].Food Ind Home Abroad,2013,(12):23-24.
[13]王利刚,张磊,张婧,等.国标培养法与2种金黄色葡萄球菌快速筛检方法的比较[J].食品安全质量检测学报,2017,8(1):238-242.Wang LG,Zhang L,Zhang J,et al.Comparison of national standard culture method and two rapid screening methods for Staphylococcus aureus[J].J Food Saf Qual,2017,8(1):238-242.
[14]卢天舒,关靖宇,安春丽.凝固酶阴性葡萄球菌临床流行及其耐药性研究现状[J].临床军医杂志,2010,38(3):451-453.Lu TS,Guan JY,An CL,Current status of clinical epidemiology and drug resistance of coagulase-negative staphylococci[J].J Clin Milit Med,2010,38(3):451-453.
[15]刘真真,林涛,李光伟.金黄色葡萄球菌不同培养与反应条件对其血浆凝固酶的影响[J].检验检疫科学,2005,15(5):34-35.Liu ZZ,Li T,Li GW.Effects of different culture and reaction conditions on plasma coagulase of Staphylococcus aureus[J].Inspect Quarant Sci,2005,15(5):34-35.
[16]时威,卢勉飞,蔡芷荷,等.冻干兔血浆在金黄色葡萄球菌检验中的应用研究[J].食品与发酵科技,2012,48(6):44-47Shi W,Lu MF,Cai ZH,et al.Application of lyophilized rabbit plasma in the detection of Staphylococcus aureus[J].Food Ferment Technol,2012,48(6):44-47.
[17]张晓梅,白淑晶.血浆凝固酶试验影响因素研究[J].中国热带医学,2005,5(1):103-104.Zhang XM,Bai SJ.Study on the influencing factors of plasma coagulase test[J].Chin Trop Med,2005,5(1):103-104.
[18]饶名祯,顾晨荣,李云霞.MALDI-TOF MS技术在食品微生物领域的应用研究[J].上海师范大学学报(自然科学版),2015,44(6):681-686.Rao MZ,Gu CR,Li YX.Application of MALDI-TOF MS in the field of food microorganisms[J].J Shanghai Norm Univ(Nat Sci Ed),2015,44(6):681-686.
[2]Fratamico PM,Arun KB,James LS.Foodborne pathogens:microbiology and molecular biology[M].Wymondham:Horizon Scientific Press,2005.
[3]Qi Y,Miller KJ.Effect of low water activity on staphylococcal enterotoxin A and B biosynthesis[J].J Food Protect,2000,63(4):473-478.
[4]李少茵,刘赐敏,陈林珍,等.金黄色葡萄球菌鉴定影响因素的研究[J].现代预防医学,2006,33(16):993-995.Li SY,Liu CM,Chen LZ,et al.Studies on the influencing factors of Staphylococcus aureus identification[J].Mod Prev Med,2006,33(16):993-995.
[5]黄汝添,吴清平,张菊梅,等.金黄色葡萄球菌显色培养基研究进展[J].中国卫生检验杂志,2007,17(6):1148-1150.Huang RT,Wu QP,Zhang JM,et al.Progress in Staphylococcus aureus colour medium[J].Chin J Health Lab Technol,2007,17(6):1148-1150.
[6]Atanassova V,Meindl A,Ring C.Prevalence of Staphylococcus aureus and staphylococcal enterotoxins in raw pork and uncookedsmoked ham-acomparison of classical culturing detection and RFLP-PCR[J].Int J Food Microbiol,2001,68(1/2):105-113.
[7]何淑华,邓艳,戴晶,等.广州市白云口岸航空食品中金黄色葡萄球菌凝固酶基因分型及肠毒素基因研究[J].中国食品卫生杂志,2018,30(4):362-367.He SH,Deng Y,Dai J,et al.Genotyping and enterotoxin gene of Staphylococcus aureus coagulase in airline food at baiyun port,Guangzhou[J].Chin J Food Hyg,2018,30(4):362-367.
[8]王英杰,王长法,陈营.PCR扩增血浆凝固酶基因检测致病性金黄色葡萄球菌[J].生物技术通报,2008,(5):185-188.Wang YJ,Wang CF,Chen Y.Detection of pathogenic Staphylococcus aureus by PCR amplification of plasma coagulase gene[J].Biotechnol Bull,2008,(5):185-188.
[9]容艳芬,李艳嫦,卢勉飞,等.兔血浆纤维蛋白原琼脂培养基技术与Baird-Parker琼脂培养基技术检测食品中金黄色葡萄球菌的比较[J].中国卫生检验杂志,2017,25(7):642-648.Rong YF,Li YC,Lu MF,et al.Comparison of rabbit plasma fibrinogen agar medium and Baird-Parker agar medium for detection of Staphylococcus aureus in food[J].Chin J Health Lab Technol,2017,25(7):642-648.
[10]British Standards Committee.ISO6888-2:1999 Horizontal method for the enumeration of coagulase-positive staphylococci(Staphylococcus aureus and other species)-Part2:Technique using rabbit plasma fibrinogen agar medium[Z].1999-05-15.
[11]GB 4789.10-2016食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S].GB 4789.10-2016 National food safety standard-Food microbiology inspection Staphylococcus aureus inspection[S].
[12]李海丽,柯娴,邓金兰,等.沙门氏菌检测方法的优化[J].中外食品工业,2013,(12):23-24.Li HL,Ke X,Deng JL,et al.Optimization of Salmonella detection method[J].Food Ind Home Abroad,2013,(12):23-24.
[13]王利刚,张磊,张婧,等.国标培养法与2种金黄色葡萄球菌快速筛检方法的比较[J].食品安全质量检测学报,2017,8(1):238-242.Wang LG,Zhang L,Zhang J,et al.Comparison of national standard culture method and two rapid screening methods for Staphylococcus aureus[J].J Food Saf Qual,2017,8(1):238-242.
[14]卢天舒,关靖宇,安春丽.凝固酶阴性葡萄球菌临床流行及其耐药性研究现状[J].临床军医杂志,2010,38(3):451-453.Lu TS,Guan JY,An CL,Current status of clinical epidemiology and drug resistance of coagulase-negative staphylococci[J].J Clin Milit Med,2010,38(3):451-453.
[15]刘真真,林涛,李光伟.金黄色葡萄球菌不同培养与反应条件对其血浆凝固酶的影响[J].检验检疫科学,2005,15(5):34-35.Liu ZZ,Li T,Li GW.Effects of different culture and reaction conditions on plasma coagulase of Staphylococcus aureus[J].Inspect Quarant Sci,2005,15(5):34-35.
[16]时威,卢勉飞,蔡芷荷,等.冻干兔血浆在金黄色葡萄球菌检验中的应用研究[J].食品与发酵科技,2012,48(6):44-47Shi W,Lu MF,Cai ZH,et al.Application of lyophilized rabbit plasma in the detection of Staphylococcus aureus[J].Food Ferment Technol,2012,48(6):44-47.
[17]张晓梅,白淑晶.血浆凝固酶试验影响因素研究[J].中国热带医学,2005,5(1):103-104.Zhang XM,Bai SJ.Study on the influencing factors of plasma coagulase test[J].Chin Trop Med,2005,5(1):103-104.
[18]饶名祯,顾晨荣,李云霞.MALDI-TOF MS技术在食品微生物领域的应用研究[J].上海师范大学学报(自然科学版),2015,44(6):681-686.Rao MZ,Gu CR,Li YX.Application of MALDI-TOF MS in the field of food microorganisms[J].J Shanghai Norm Univ(Nat Sci Ed),2015,44(6):681-686.