文蛤生物活性多肽/蛋白研究进展
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摘要
文蛤(Meretrix meretrix)是一种重要的海洋贝类,其肉质鲜美、营养丰富,含有多种人体必需氨基酸、微量元素、维生素和其它活性物质。研究发现,文蛤多肽、蛋白和酶具有多种生物功能,如免疫调节、抗氧化、抗菌、消炎、抗脂质、抗衰老、降血糖活性、抗高血压和红细胞凝集活性及预防癌症、肝损伤保护作用。总结了文蛤生物活性多肽、蛋白质、酶及酶抑制剂的最新研究进展,并对下一步研究方向进行了展望,旨在为文蛤活性多肽/蛋白利用的深入研究提供参考。
Marine organisms,which make up approximately one half of the total global biodiversity,are rich reservoirs of structurally diverse biofunctional components. In particular,some of these bioactive compounds are proteinaceous in nature and include proteins,peptides and amino acids. The clam Meretrix meretrix( M.meretrix) belonging to the genus Meretrix and family Veneridae is economically very important marine eurythermal and eurysaline benthic bivalves,and is widely distributed in the eastern Asia. It is an important commercial mudflat shellfish in China. It tastes delicious and contains carbohydrates,protein,and vitamins.M. meretrix is a historically marine food and a valuable source of traditional Chinese medicine( TCM).Documented in the ancient Chinese pharmacopeia Compendium of Material Medica( the 16 th century,by Li Shizhen),M. meretrix could diminish inflammation,treat typhoid fever,hangover and relieve pain. Modern research has verified the effects of M. meretrix on immunomodulatory,antioxidant,antitumor and cancer preventive,antimicrobial activity,antiviral,anti-inflammatory,antioxidant,antilipidaemic,antiaging effects,hypoglycaemic activities,antihypertensive activity,erythrocyte agglutination activity and protective effect on acute liver injury. This article reviews the current knowledge of the existence of peptides/proteins from M.meretrix,such as antimicrobial peptides,antioxidant peptides,ACE inhibitory peptides,immunomodulatory peptides etc.,enzymes and inhibitors. This review also describes various manufacturing techniques for bioactive peptides/proteins using ammonium sulfate precipitation, chemical hydrolysis, and enzymes hydrolysis. Finally,purification methods of bioactive peptides/proteins are described,including gel or size exclusion chromatography,ion-exchange column chromatography,and reversed-phase high-performance liquid chromatography.
Marine organisms,which make up approximately one half of the total global biodiversity,are rich reservoirs of structurally diverse biofunctional components. In particular,some of these bioactive compounds are proteinaceous in nature and include proteins,peptides and amino acids. The clam Meretrix meretrix( M.meretrix) belonging to the genus Meretrix and family Veneridae is economically very important marine eurythermal and eurysaline benthic bivalves,and is widely distributed in the eastern Asia. It is an important commercial mudflat shellfish in China. It tastes delicious and contains carbohydrates,protein,and vitamins.M. meretrix is a historically marine food and a valuable source of traditional Chinese medicine( TCM).Documented in the ancient Chinese pharmacopeia Compendium of Material Medica( the 16 th century,by Li Shizhen),M. meretrix could diminish inflammation,treat typhoid fever,hangover and relieve pain. Modern research has verified the effects of M. meretrix on immunomodulatory,antioxidant,antitumor and cancer preventive,antimicrobial activity,antiviral,anti-inflammatory,antioxidant,antilipidaemic,antiaging effects,hypoglycaemic activities,antihypertensive activity,erythrocyte agglutination activity and protective effect on acute liver injury. This article reviews the current knowledge of the existence of peptides/proteins from M.meretrix,such as antimicrobial peptides,antioxidant peptides,ACE inhibitory peptides,immunomodulatory peptides etc.,enzymes and inhibitors. This review also describes various manufacturing techniques for bioactive peptides/proteins using ammonium sulfate precipitation, chemical hydrolysis, and enzymes hydrolysis. Finally,purification methods of bioactive peptides/proteins are described,including gel or size exclusion chromatography,ion-exchange column chromatography,and reversed-phase high-performance liquid chromatography.
引文
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[2]李树国,丰浪,沈崇明,等.崇明养殖文蛤和启东天然生长文蛤的营养成分比较[J].营养学报,2009,31(6):612-613.LI S G, FENG L, SHEN C M, et al. The comparison of nutritional composition of Meretrix meretrix collected in Chongming and Qidong[J].Acta Nutrimenta Sinica,2009,31(6):612-613.
[3] KARNJANAPRATUM S,BENJAKUL S,KISHIMURA H,et al. Chemical compositions and nutritional value of Asian hard clam(Meretrix lusoria)from the coast of Andaman Sea[J]. Food Chemisty,2013,141(4):4138-4145.
[4]赵莎莎,赵玉勤,张亚茹,等.文蛤多肽的制备及对非酒精性脂肪肝细胞模型的修复作用[J].中国食品学报,2017,17(6):33-39.ZHAO S S,ZHAO Y Q,ZHANG Y R,et al.Preparation of NAFLD cell model repair polypeptide from Meretrix meretrix[J]. Journal of Chinese Institute of Food Science and Technology,2017,17(6):33-39.
[5] LIU Z Y,ZHOU D Y,WU Z X,et al. Extraction and detailed characterization of phospholipidenriched oils from six species of edible clams[J].Food Chemisty,2018(239):1175-1181.
[6] SCHMEER M R. Growth-inhibiting agents from mercenaria extracts:Chemical and biological properties[J]. Science,1964,144(3617):413-414.
[7] WANG C,LIU M,CHENG L,et al. A novel polypeptide from Meretrix meretrix Linnaeus inhibits the growth of human lung adenocarcinoma[J].Experimental Biology and Medicine,2012,237(4):442-450.
[8] SATHYAN N,PHILIP R,CHAITHANYA E R,et al. Identification and molecular characterization of molluskin, a Histone-H2A-derived antimicrobial peptide from molluscs[J]. ISRN Molecular Biology,2012:219656.
[9]李和生,刘智勇,王鸿飞.文蛤多肽组分的分离及其抗氧化活性研究[J].中国食品学报,2012,12(6):30-35.LI H S, LIU Z Y, WANG H F. Isolation of polypeptide components of Meretrix meretrix Linnaeus and their antioxidant activities[J]. Journal of Chinese Institute of Food Science and Technology,2012,12(6):30-35.
[10]张晶晶,李宏俊,秦艳杰,等.文蛤C型凝集素基因(Mm-Lec1)的克隆与表达分析[J].海洋学报,2016,38(6):110-118.ZHANG J J,LI H J,QIN Y J,et al. Cloning and expression and expression analysis of a C-type lectin gene(Mm-Lec1)in hard clam Meretrix meretrix[J].Acta Oceanologica Sinica,2016,38(6):110-118.
[11]蒋国萍,程雪艳,滕爽爽,等.斧文蛤金属硫蛋白基因的克隆与表达分析[J].水生生物学报,2016,40(5):914-920.JIANG G P,CHENG X Y,TENG S S,et al.Cloning and expression of metallothionein gene in Meretrix lamarckii[J]. Acta Hydrobiologica Sinica,2016,40(5):914-920.
[12] CHEN H,YANG X,TANG T,et al. The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam,Meretrix meretrix[J]. Fish and Shellfish Immunology,2014,40(2):435-440.
[13] CHENG C F,HUNG S W,CHANG Y C,et al.Purification and characterization of hemagglutinating proteins from poker-chip venus(Meretrix lusoria)and Corbicula clam(Corbicula fluminea)[J].Scientific World Journal,2012:906737.
[14] LIU S X, QI Z H, ZHANG J J, et al.Lipopolysaccharide andβ-1,3-glucan binding protein in the hard clam(Meretrix meretrix)molecular characterization and expression analysis[J].Genetics and Molecular Research,2014,13(3):4956-4966.
[15]杨杰青,沈新强,蒋玫,等.盐度、p H对文蛤肌肉、鳃、外套膜HSP70基因表达的影响[J].水产科学,2016,35(4):398-403.YANG J Q,SHEN X Q,JIANG M,et al. Effects of salinity and p H on expression of HSP70 gene in muscle,gill and mantle of clam Meretrix meretrix[J]. Fisheries Science,2016,35(4):398-403.
[16] NAKANO T,MATSUSHIMA-HIBIYA Y,YAMAMOTO M. Purification and molecular cloning of a DNA ADP-ribosylating protein,CARP-1,from the edible clam Meretrix lamarckii[J]. Proceedings of the National Academy of Sciences USA, 2006, 103(37):13652-13657.
[17] LI H J,YANG Q,GAO X G,et al. Identification and expression of a putative LPS-induced TNF-a factor from Asiatic hard clam Meretrix meretrix[J].Molecular Biology Reports,2012,39(2):865-871.
[18] HUANG F,ZHAO S,YU F,et al. Protective effects and mechanism of Meretrix meretrix oligopeptides against nonalcoholic fatty liver disease[J]. Marine Drugs,2017,15(2),pii:E31.
[19]王佳佳,叶蕾,杨最素,等.文蛤寡肽对高脂饮食诱导小鼠肾损伤的修护作用[J].食品科学,2017,39(15):145-151WANG J J,YE L,YANG Z S,et al. Protective effect of Meretrix meretrix oligopeptides on kidney injury induced by high fat diet[J]. Food Science,2017,39(15):145-151.
[20] MANIKKAM V,VASILJEVIC T,DONKOR O N,et al. A review of potential marine-derived hypotensive and anti-obesity peptides[J]. Critical Reviews in Food Science and Nutrition,2016,56(1):92-112.
[21] SCHMEER M R. Growth-inhibiting agents from mercenaria extracts:Chemical and biological properties[J]. Science,1964,144(3617):413-414.
[22] ZHENG L H,WANG Y J,SHENG J,et al. Antitumor peptides from marine organisms[J]. Marine Drugs,2011,9(10):1840-1859.
[23] NING X,ZHAO J,ZHANG Y,et al. A novel antitumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization[J]. International Journal of Oncology,2009,35(4):805-812.
[24] WANG H,WEI J T,WU N,et al. Mere15,a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs[J].Pharmaceutical Biology,2013,51(2):145-151.
[25] LIU M,ZHAO X,ZHAO J,et al. Induction of apoptosis, G0/G1 phase arrest and microtubule disassembly in K562 leukemia cells by Mere15,a novel polypeptide from Meretrix meretrix Linnaeus[J]. Marine Drugs,2012,10(11):2596-2607.
[26] NEGI B,KUMAR D,RAWAT D S. Marine peptides as anticancer agents:A remedy to mankind by nature[J]. Current Protein and Peptide Science,2017,18(9):885-904.
[27]吴桐.动物药抗肿瘤药理活性研究进展[J].亚太传统医药,2017,13(18):81-85.WU T. Research progress in antitumor pharmacological activity of animal drugs[J]. AsiaPacific Traditional Medicine,2017,13(18):81-85.
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