巴什拜羊与其杂交羊对绵羊肺炎支原体感染的比较
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摘要
为了比较巴什拜羊与其杂交羊对绵羊肺炎支原体(MO)感染的差异,将6只巴什拜羊和6只杂交羊人工感染MO,分别观察其临床症状和病理解剖变化,用ELISA方法分别检测血清中IL-1β、IL-6、IL-9及IFN-γ的浓度,制作肺部组织切片观察组织病理学变化,并进行组织病理学评分。结果显示,感染后杂交羊比巴什拜羊表现出更严重的、典型的支原体肺炎的临床症状和病理剖检变化。组织病理学评分结果显示,杂交羊的评分显著高于巴什拜羊。相关细胞因子检测结果显示,杂交羊血清中IL-1β、IL-6、IL-9及IFN-γ的浓度均显著高于巴什拜羊。结果表明,巴氏拜羊对MO感染具有一定的抗性,而杂交羊对MO较易感。
In order to compare the difference of Mycoplasma ovipneumoniae(MO)infection between Bashbay sheep and its crossbred sheep,6Bashbay sheep and 6hybrid sheep were infected with MO,and their clinical symptoms and pathological lesions were observed.IL-1β,IL-6,IL-9and IFN-γconcentrations in sera were measured by ELISA.Histopathological lesions of lung tissues were observed by pathological sections and histopathological scores were obtained.The results showed that the post-infection hybrid sheep represented more severe,typical mycoplasma pneumonia clinical symptoms and pathological lesions than Bashbay sheep.The results of histopathological scoring found that the hybrid sheep was significantly higher than Bashbay sheep.The results of cytokine test showed that the concentrations of IL-1β,IL-6,IL-9and IFN-γin sera of hybrid sheep were significantly higher than those of Bashbay sheep.Bashbay sheep has certain resistance to MO infection,while hybrid sheep was more susceptible to MO.
In order to compare the difference of Mycoplasma ovipneumoniae(MO)infection between Bashbay sheep and its crossbred sheep,6Bashbay sheep and 6hybrid sheep were infected with MO,and their clinical symptoms and pathological lesions were observed.IL-1β,IL-6,IL-9and IFN-γconcentrations in sera were measured by ELISA.Histopathological lesions of lung tissues were observed by pathological sections and histopathological scores were obtained.The results showed that the post-infection hybrid sheep represented more severe,typical mycoplasma pneumonia clinical symptoms and pathological lesions than Bashbay sheep.The results of histopathological scoring found that the hybrid sheep was significantly higher than Bashbay sheep.The results of cytokine test showed that the concentrations of IL-1β,IL-6,IL-9and IFN-γin sera of hybrid sheep were significantly higher than those of Bashbay sheep.Bashbay sheep has certain resistance to MO infection,while hybrid sheep was more susceptible to MO.
引文
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[13]Shao L,Cong Z,Li X,et al.Changes in levels of IL-9,IL-17,IFN-γ,dendritic cell numbers and TLR expression in peripheral blood in asthmatic children with Mycoplasma pneumoniae infection[J].Int J Clin Exp Pathol,2014,8(5):5263-5272.
[14]王建勇,郑静,邢海燕,等.Th9细胞与IL-9在肺炎支原体感染患儿中的检测及其临床意义[J].中国当代儿科杂志,2015(4):308-311.
[15]邵莉,曹兰芳,李晓丽,等.支气管哮喘合并肺炎支原体感染小鼠模型白介素9、白介素17、γ干扰素的变化[J].内科理论与实践,2016,11(1):47-51.
[16]Tanaka H,Narita M,Teramoto S,et al.Role of interleukin-18and T-helper type 1cytokines in the development of Mycoplasma pneumoniae pneumonia in adults[J].Chest,2002,121:1493-1497.
[17]Yang J,Hooper W C,Phillips D J,et al.Cytokines in Mycoplasma pneumoniae infections[J].Cytokine&Growth Factor Reviews,2004,15(2):157-168.
[18]高伟.肺炎支原体感染的临床治疗分析及免疫学致病机制研究[D].天津:天津医科大学,2011.
[2]决肯·阿尼瓦尔,韩业东,李齐发,等.巴什拜羊微卫星标记多态性及其与生长指标关联性分析[J].中国农业科学,2010,43(16):3425-3432.
[3]马长宾,孙延鸣,朱晓光,等.新疆野生盘羊与巴什拜羊杂交一代生产性能测定[J].畜禽业,2009(2):44-45
[4]姜方配,沈文,鲁海富,等.ISG15蛋白对感染绵羊肺炎支原体的盘羊杂交羊的免疫调节作用[J].中国预防兽医学报,2013,35(11):925-928.
[5]张慧,赵宗胜,赵凤,等.绵羊支原体肺炎中不同甘露(聚)糖结合凝集素基因型对肺组织病变的影响[J].中国预防兽医学报,2012,34(4):293-296.
[6]海拉提·库尔曼,依明·苏来曼,杜曼,等.巴什拜羊与野生盘羊后代杂种的适应性分析[J].新疆农业大学学报,2012,35(2):129-131.
[7]郭海英,王明哲,陈冬梅,等.绵羊肺炎支原体感染羊细胞因子的动态变化[J].畜牧与兽医,2015,47(8):1-4.
[8]Yang J,Hooper W C,Phillips D J,et al.Regulation of proinflammatory cytokines in human lung epithelial cells infected with Mycoplasma pneumoniae[J].Infect Immun,2002,70:3649-3655.
[9]Yang J,Hooper W C,Phillips D J,et al.Interleukin-1beta responses to Mycoplasma pneumoniae infection are cell-type specific[J].Microb Pathog,2003,34:17-25.
[10]Pietsch K,Ehlers S,Jacobs E.Cytokine gene expression in the lungs of BALB/c mice during primary and secondary intranasal infection with Mycoplasma pneumoniae[J].Microbiology,1994,140(8):2043-2048.
[11]Reinhart K,Menges T,Cardlund B,et al.Randomized,placebo-controlled trial of the antitumor necrosis factor antibody fragment a felmiomab in hyper inflammatory response during severe sepsis:The RAM SES Study[J].Crit Care Med,2001,(29):765-769.
[12]Veldhoen M,Uyttenhove C,van Snick J,et al.Transforming growth factor-beta'reprograms'the differentiation of T helper2cells and promotes an interleukin 9-producing subset[J].Nat Immunol,2008,9(12):1341-1346.
[13]Shao L,Cong Z,Li X,et al.Changes in levels of IL-9,IL-17,IFN-γ,dendritic cell numbers and TLR expression in peripheral blood in asthmatic children with Mycoplasma pneumoniae infection[J].Int J Clin Exp Pathol,2014,8(5):5263-5272.
[14]王建勇,郑静,邢海燕,等.Th9细胞与IL-9在肺炎支原体感染患儿中的检测及其临床意义[J].中国当代儿科杂志,2015(4):308-311.
[15]邵莉,曹兰芳,李晓丽,等.支气管哮喘合并肺炎支原体感染小鼠模型白介素9、白介素17、γ干扰素的变化[J].内科理论与实践,2016,11(1):47-51.
[16]Tanaka H,Narita M,Teramoto S,et al.Role of interleukin-18and T-helper type 1cytokines in the development of Mycoplasma pneumoniae pneumonia in adults[J].Chest,2002,121:1493-1497.
[17]Yang J,Hooper W C,Phillips D J,et al.Cytokines in Mycoplasma pneumoniae infections[J].Cytokine&Growth Factor Reviews,2004,15(2):157-168.
[18]高伟.肺炎支原体感染的临床治疗分析及免疫学致病机制研究[D].天津:天津医科大学,2011.