感染绵羊肺炎支原体后绵羊SPLUNC1 mRNA表达水平监测
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摘要
为检测绵羊感染绵羊肺炎支原体(MO)前后的短腭、肺及鼻咽上皮克隆基因1(SPLUNC1)表达水平变化,对6只巴什拜羊和6只盘羊杂交羊分别人工感染MO,在感染前(第0天)和感染后第5、14、21天,采集口腔喉咽部的上腭组织,用Real-time PCR方法检测SPLUNC1mRNA的表达水平。结果:感染后两组羊SPLUNC1 mRNA的相对表达水平均升高,在感染后第14~21天,巴什拜羊SPLUNC1 mRNA水平均极显著高于盘羊杂交羊(P<001)。该结果说明在临床上表现为抗MO感染的巴什拜羊,体内可表达高水平的SPLUNC1 mRNA,这为巴什拜羊抗MO感染的机理研究提供一定的参考依据。
The aim of this study was to detect the expression level of short palate, lung and nasal epithelium clone 1(SPLUNC1) inBashibai sheep(BS) and Argali hybrid sheep(AHS) infected withMycoplasma ovipneumoniae(MO). All the sheep were artificially in-fected with MO. Then the oral pharynx and larynx of palate tissue was collected before infection(0 d) and on the 5, 14, 21 days after infec-tion(dpi), and the expression level of SPLUNC1 mRNA was detected by Real-time PCR. The results showed that after infection, theSPLUNC1 expression levels in the sheep from the two groups were increased. On the 14 to 21 dpi, the SPLUNC1 levels in BS sheep weresignificantly higher than those in AHS sheep(P<0 01). The results showed that in the BS sheep showing anti-MO infection, the high levelof SPLUNC1 mRNA can be expressed, which provides a reference for investigation on the mechanism of anti-MO infection in BS sheep.
The aim of this study was to detect the expression level of short palate, lung and nasal epithelium clone 1(SPLUNC1) inBashibai sheep(BS) and Argali hybrid sheep(AHS) infected withMycoplasma ovipneumoniae(MO). All the sheep were artificially in-fected with MO. Then the oral pharynx and larynx of palate tissue was collected before infection(0 d) and on the 5, 14, 21 days after infec-tion(dpi), and the expression level of SPLUNC1 mRNA was detected by Real-time PCR. The results showed that after infection, theSPLUNC1 expression levels in the sheep from the two groups were increased. On the 14 to 21 dpi, the SPLUNC1 levels in BS sheep weresignificantly higher than those in AHS sheep(P<0 01). The results showed that in the BS sheep showing anti-MO infection, the high levelof SPLUNC1 mRNA can be expressed, which provides a reference for investigation on the mechanism of anti-MO infection in BS sheep.
引文
[1]Dassanayake R P,Shanthalingam S,Herndon C N,et al.Mycoplasma ovipneumoniaecan predispose bighorn sheep to fatalMannheimia haemolyticapneumonia[J].Vet Microbiol,2010,145(3-4):354-359.
[2]尹正军,岳华,汤承.绵羊肺炎支原体延伸因子Tu基因的分子特性分析[J].畜牧兽医学报,2015,46(2):288-294.
[3]Raghavan B,Erickson K,Kugadas A,et al.Role of carriers in the transmission of pneumonia in bighorn sheep(Ovis canadensis)[J].Biology Open,2016,5(6):745-755.
[4]马长宾,孙延鸣,朱晓光,等.新疆野生盘羊与巴什拜羊杂交一代生产性能测定[J].畜禽业,2009,(2):44-45.
[5]李志远,冷青文,鲁海富.杂交盘羊绵羊传染性胸膜肺炎支原体的诊断及防控[J].新疆畜牧业,2011(11):54-56.
[6]姜方配,沈文,鲁海富,等.ISG15蛋白对感染绵羊肺炎支原体的盘羊杂交羊的免疫调节作用[J].中国预防兽医学报,2013,35(11):925-928.
[7]Walton W G,Ahmad S,Little M S,et al.Structural features essential to the antimicrobial functions of human SPLUNC1[J].Biochemistry,2016,55(21):2979-2991.
[8]张晓丽,高宝德,刘海燕,等.巴什拜羊重组SPLUNC1蛋白对体外培养的绵羊肺炎支原体生长的影响[J].中国预防兽医学报,2016,38(5):361-365.
[9]Fabienne Gally,Y Peter Di,Sean K Smith,et al.SPLUNC1 promotes lung innate defense againstMycoplasma pneumoniaeinfection in mice[J].Am J Pathol,2011,178(5):2159-2167.
[10]郭海英,王明哲,陈冬梅,等.绵羊感染支原体后杀菌通透性增加蛋白(BPI)mRNA表达水平的变化[J].畜牧与兽医,2015,47(9):35-37.
[11]陈凯丽.绵羊SPLUNC1基因的克隆、表达及活性检测[D].石河子:石河子大学,2015.
[12]Bingle C D,Seal R L,Craven C J.Systematic nomenclature for the PLUNC/PSP/BSP30/SMGB proteins as a subfamily of the BPI fold-containing superfamily[J].Biochemical Society Transactions,2011,39(4):977-983.
[13]Zhou H D,Fan S Q,Jin Z,et al.Tissue distribution of the secretory protein,SPLUNC1,in the human fetus[J].Histochemistry and Cell Biology,2006,125(3):315-324.
[14]王爽,李文路,吕丽春,等.SPLUNC1基因在人体组织中的表达分布特征[J].南方医科大学学报,2016,36(5):617-621.
[2]尹正军,岳华,汤承.绵羊肺炎支原体延伸因子Tu基因的分子特性分析[J].畜牧兽医学报,2015,46(2):288-294.
[3]Raghavan B,Erickson K,Kugadas A,et al.Role of carriers in the transmission of pneumonia in bighorn sheep(Ovis canadensis)[J].Biology Open,2016,5(6):745-755.
[4]马长宾,孙延鸣,朱晓光,等.新疆野生盘羊与巴什拜羊杂交一代生产性能测定[J].畜禽业,2009,(2):44-45.
[5]李志远,冷青文,鲁海富.杂交盘羊绵羊传染性胸膜肺炎支原体的诊断及防控[J].新疆畜牧业,2011(11):54-56.
[6]姜方配,沈文,鲁海富,等.ISG15蛋白对感染绵羊肺炎支原体的盘羊杂交羊的免疫调节作用[J].中国预防兽医学报,2013,35(11):925-928.
[7]Walton W G,Ahmad S,Little M S,et al.Structural features essential to the antimicrobial functions of human SPLUNC1[J].Biochemistry,2016,55(21):2979-2991.
[8]张晓丽,高宝德,刘海燕,等.巴什拜羊重组SPLUNC1蛋白对体外培养的绵羊肺炎支原体生长的影响[J].中国预防兽医学报,2016,38(5):361-365.
[9]Fabienne Gally,Y Peter Di,Sean K Smith,et al.SPLUNC1 promotes lung innate defense againstMycoplasma pneumoniaeinfection in mice[J].Am J Pathol,2011,178(5):2159-2167.
[10]郭海英,王明哲,陈冬梅,等.绵羊感染支原体后杀菌通透性增加蛋白(BPI)mRNA表达水平的变化[J].畜牧与兽医,2015,47(9):35-37.
[11]陈凯丽.绵羊SPLUNC1基因的克隆、表达及活性检测[D].石河子:石河子大学,2015.
[12]Bingle C D,Seal R L,Craven C J.Systematic nomenclature for the PLUNC/PSP/BSP30/SMGB proteins as a subfamily of the BPI fold-containing superfamily[J].Biochemical Society Transactions,2011,39(4):977-983.
[13]Zhou H D,Fan S Q,Jin Z,et al.Tissue distribution of the secretory protein,SPLUNC1,in the human fetus[J].Histochemistry and Cell Biology,2006,125(3):315-324.
[14]王爽,李文路,吕丽春,等.SPLUNC1基因在人体组织中的表达分布特征[J].南方医科大学学报,2016,36(5):617-621.