Zebularine对乳腺癌细胞株MDA-MB-231细胞增殖和SARI表达的影响
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摘要
目的:探讨Zebularine对乳腺癌细胞株MDA-MB-231细胞增殖和SARI表达的影响。方法:选用MDA-MB-231细胞株,分别通过不同浓度的Zebularine作用细胞株24 h后收集细胞,运用MTT法测定Zebularine对乳腺癌细胞株MDA-MB-231细胞的增殖;通过Western Blot检测Zebularine对乳腺癌细胞株SARI的表达。结果:随着药物浓度的增加,Zebularine能够明显抑制乳腺癌细胞株MDA-MB-231的增殖(P<0.05),Zebularine能够显著提高乳腺癌细胞株MDA-MB-231 SARI蛋白的表达(P<0.05)。结论:Zebularine通过抑制乳腺癌细胞增殖,促进SARI蛋白的表达,从而发挥肿瘤抑制作用。
Objective:To investigate the effect of Zebularine on the proliferation and SARI expression of human breast cancer cell line MDA-MB-231.Method:MDA-MB-231 cell line were cultured in vitro and treated with different doses Zebularine for 24 hours.MTT method was used to detect the cell proliferation,and western blot was used to detect the expression of SARI in human breast cancer cell line MDA-MB-231.Result:Different doses Zebularine could significantly inhibit the proliferation of human breast cancer cell line MDA-MB-231 with the increase of drug concentration(P<0.05).Zebularine could obviously reduced the expression of SARI in human breast cancer cell line MDA-MB-231(P<0.05).Conclusion:Zebularine might inhibit the proliferation,improve the expression of SARI,then inhibit the malignant proliferation of tumor cells,then exerting tumor inhibitory effect.
Objective:To investigate the effect of Zebularine on the proliferation and SARI expression of human breast cancer cell line MDA-MB-231.Method:MDA-MB-231 cell line were cultured in vitro and treated with different doses Zebularine for 24 hours.MTT method was used to detect the cell proliferation,and western blot was used to detect the expression of SARI in human breast cancer cell line MDA-MB-231.Result:Different doses Zebularine could significantly inhibit the proliferation of human breast cancer cell line MDA-MB-231 with the increase of drug concentration(P<0.05).Zebularine could obviously reduced the expression of SARI in human breast cancer cell line MDA-MB-231(P<0.05).Conclusion:Zebularine might inhibit the proliferation,improve the expression of SARI,then inhibit the malignant proliferation of tumor cells,then exerting tumor inhibitory effect.
引文
[1]Fan L,Strasserweippl K,Li J J,et al.Breast cancer in China.[J].Lancet Oncology,2014,15(7):e279-e289.
[2]黄庆,李小青,杨琰,等.BCR-ABL抑制剂STI571对慢性髓系白血病细胞株K562中SARI基因表达影响的研究[J].中国实验血液学杂志,2011,19(4):865-868.
[3]Shenker N,Flanagan J M.Intragenic DNA methylation:implications of this epigenetic mechanism for cancer research[J].British Journal of Cancer,2012,106(2):248-253.
[4]邹惠,贺湘玲,黄淑桂,等.Zebularine对HL-60及K562细胞株WWOX基因甲基化的影响[J].湖南师范大学学报(医学版),2014,11(3):5-9
[5]马静.SHP2调控乳腺癌细胞增殖的分子机制研究[D].天津医科大学,2018.
[6]Desantis C E,Ma J,Goding S A,et al.Breast cancer statistics,2017,racial disparity in mortality by state[J].Ca Cancer J Clin,2017,67(6):439-448.
[7]Siegel R L,Miller K D,Jemal A.Cancer statistics,2018[J].Ca ACancer Journal for Clinicians,2018,60(5):277-300.
[8]Chen W,Zheng R,Baade P D,et al.Cancer statistics in China,2015[J].Ca Cancer J Clin,2016,66(2):115-132.
[9]姜青明,周文文,李光新,等.miR-126靶向EGFL7抑制乳腺癌细胞MDA-MB-231上皮-间质转化[J].临床与病理杂志,2018,38(10):2055-2062
[10]Wang Y,Lin Z P,Zheng H Z,et al.Abnormal DNAmethylation in the lumbar spinal cord following chronic constriction injury in rats[J].Neuroscience Letters,2016,610:1-5.
[11]Hakata S,Terashima J,Shimoyama Y,et al.Differential sensitization of two human colon cancer cell lines to the antitumor effects of irinotecan combined with 5-aza-2'-deoxycytidine[J].Oncology Letters,2018,15(4):4641-4648.
[12]Tanaka S,Hosokawa M,Matsumura J,et al.Effects of Zebularine on Invasion Activity and Intracellular Expression Level of let-7b in Colorectal Cancer Cells[J].Biological&Pharmaceutical Bulletin,2017,40(8):1320-1325.
[13]张征,陈先祥,董荣坤,等.zebularine对人胃癌SGC-7901细胞株抑癌基因p16表达的影响[J].中国普通外科杂志,2015,24(6):889-891.
[14]Maldonado L,Hoque M O.Epigenomics and ovarian carcinoma[J].Biomarkers in Medicine,2010,4(4):543-570.
[15]Yang P M,Lin Y T,Shun C T,et al.Zebularine inhibits tumorigenesis and stemness of colorectal cancer via p53-dependent endoplasmic reticulum stress[J].Scientific Reports,2013,3:3219.
[16]熊辉,丁佑铭,廖晓峰.Zebularine对胆管癌细胞增殖、凋亡的影响及其机制[J].胃肠病学和肝病学杂志,2015,24(7):864-867.
[17]丁佑铭,於展飞,廖晓峰.Zebularine对肝癌HepG2细胞凋亡的影响及其机制[J].胃肠病学和肝病学杂志,2015,24(5):515-518.
[18]Su Z Z,Lee S G,Emdad L,et al.Cloning and characterization of SARI(suppressor of AP-1,regulated by IFN)[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(52):20906-20911.
[19]Liu Z,Yang Y,Zhou X.BATF2 in human colorectal cancer[J].Aging,2015,7(5):284-285.
[20]Zhou R J,Shi Z,Zhou K,et al.Decreased SARI expression predicts poor prognosis of Chinese patients with non-small cell lung cancer[J].International Journal of Clinical&Experimental Pathology,2013,6(10):2056-2063.
[21]龚嘉玲,白雪佳,路钊,等.AAV介导的BATF2/SARI过表达抑制肝癌细胞HepG2增殖[J].北京医学,2013,35(6):431-433.
[22]Dash R,Su Z Z,Lee S G,et al.Inhibition of AP-1 by SARInegatively regulates transformation progression mediated by CCN1[J].Oncogene,2010,29(31):4412-4423.
[23]Zhang Y X,Yan L,Liu G Y,et al.Inhibition of janus kinase2 by compound AG490 suppresses the proliferation of MDA-MB-231 cells via up-regulating SARI(suppressor of AP-1,regulated by IFN)[J].Iranian Journal of Basic Medical Sciences,2015,18(6):599-603.
[24]王平,陈祥俊,郑金娥,等.SARI基因过表达对K562细胞系生物学影响的研究[J].中国实验血液学杂志,2015,23(3):637-641.
[25]殷鹏,张月飞.Zebularine抗肿瘤研究进展[J].广东医学,2007,28(4):659-662.
[26]郑莹,吴春晓,吴凡.中国女性乳腺癌死亡现况和发展趋势[J].中华预防医学杂志,2011,45(2):150-154.
[27]左婷婷,陈万青.中国乳腺癌全人群生存率分析研究进展[J].中国肿瘤临床,2016,43(14):639-642.
[2]黄庆,李小青,杨琰,等.BCR-ABL抑制剂STI571对慢性髓系白血病细胞株K562中SARI基因表达影响的研究[J].中国实验血液学杂志,2011,19(4):865-868.
[3]Shenker N,Flanagan J M.Intragenic DNA methylation:implications of this epigenetic mechanism for cancer research[J].British Journal of Cancer,2012,106(2):248-253.
[4]邹惠,贺湘玲,黄淑桂,等.Zebularine对HL-60及K562细胞株WWOX基因甲基化的影响[J].湖南师范大学学报(医学版),2014,11(3):5-9
[5]马静.SHP2调控乳腺癌细胞增殖的分子机制研究[D].天津医科大学,2018.
[6]Desantis C E,Ma J,Goding S A,et al.Breast cancer statistics,2017,racial disparity in mortality by state[J].Ca Cancer J Clin,2017,67(6):439-448.
[7]Siegel R L,Miller K D,Jemal A.Cancer statistics,2018[J].Ca ACancer Journal for Clinicians,2018,60(5):277-300.
[8]Chen W,Zheng R,Baade P D,et al.Cancer statistics in China,2015[J].Ca Cancer J Clin,2016,66(2):115-132.
[9]姜青明,周文文,李光新,等.miR-126靶向EGFL7抑制乳腺癌细胞MDA-MB-231上皮-间质转化[J].临床与病理杂志,2018,38(10):2055-2062
[10]Wang Y,Lin Z P,Zheng H Z,et al.Abnormal DNAmethylation in the lumbar spinal cord following chronic constriction injury in rats[J].Neuroscience Letters,2016,610:1-5.
[11]Hakata S,Terashima J,Shimoyama Y,et al.Differential sensitization of two human colon cancer cell lines to the antitumor effects of irinotecan combined with 5-aza-2'-deoxycytidine[J].Oncology Letters,2018,15(4):4641-4648.
[12]Tanaka S,Hosokawa M,Matsumura J,et al.Effects of Zebularine on Invasion Activity and Intracellular Expression Level of let-7b in Colorectal Cancer Cells[J].Biological&Pharmaceutical Bulletin,2017,40(8):1320-1325.
[13]张征,陈先祥,董荣坤,等.zebularine对人胃癌SGC-7901细胞株抑癌基因p16表达的影响[J].中国普通外科杂志,2015,24(6):889-891.
[14]Maldonado L,Hoque M O.Epigenomics and ovarian carcinoma[J].Biomarkers in Medicine,2010,4(4):543-570.
[15]Yang P M,Lin Y T,Shun C T,et al.Zebularine inhibits tumorigenesis and stemness of colorectal cancer via p53-dependent endoplasmic reticulum stress[J].Scientific Reports,2013,3:3219.
[16]熊辉,丁佑铭,廖晓峰.Zebularine对胆管癌细胞增殖、凋亡的影响及其机制[J].胃肠病学和肝病学杂志,2015,24(7):864-867.
[17]丁佑铭,於展飞,廖晓峰.Zebularine对肝癌HepG2细胞凋亡的影响及其机制[J].胃肠病学和肝病学杂志,2015,24(5):515-518.
[18]Su Z Z,Lee S G,Emdad L,et al.Cloning and characterization of SARI(suppressor of AP-1,regulated by IFN)[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(52):20906-20911.
[19]Liu Z,Yang Y,Zhou X.BATF2 in human colorectal cancer[J].Aging,2015,7(5):284-285.
[20]Zhou R J,Shi Z,Zhou K,et al.Decreased SARI expression predicts poor prognosis of Chinese patients with non-small cell lung cancer[J].International Journal of Clinical&Experimental Pathology,2013,6(10):2056-2063.
[21]龚嘉玲,白雪佳,路钊,等.AAV介导的BATF2/SARI过表达抑制肝癌细胞HepG2增殖[J].北京医学,2013,35(6):431-433.
[22]Dash R,Su Z Z,Lee S G,et al.Inhibition of AP-1 by SARInegatively regulates transformation progression mediated by CCN1[J].Oncogene,2010,29(31):4412-4423.
[23]Zhang Y X,Yan L,Liu G Y,et al.Inhibition of janus kinase2 by compound AG490 suppresses the proliferation of MDA-MB-231 cells via up-regulating SARI(suppressor of AP-1,regulated by IFN)[J].Iranian Journal of Basic Medical Sciences,2015,18(6):599-603.
[24]王平,陈祥俊,郑金娥,等.SARI基因过表达对K562细胞系生物学影响的研究[J].中国实验血液学杂志,2015,23(3):637-641.
[25]殷鹏,张月飞.Zebularine抗肿瘤研究进展[J].广东医学,2007,28(4):659-662.
[26]郑莹,吴春晓,吴凡.中国女性乳腺癌死亡现况和发展趋势[J].中华预防医学杂志,2011,45(2):150-154.
[27]左婷婷,陈万青.中国乳腺癌全人群生存率分析研究进展[J].中国肿瘤临床,2016,43(14):639-642.