拟黑多刺蚁活性组分治疗小鼠皮肤瘙痒的实验研究
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摘要
目的:研究拟黑多刺蚁活性组分的止痒作用及其可能机制。方法:建立右旋糖酐诱导的小鼠瘙痒模型,观察拟黑多刺蚁活性组分对瘙痒小鼠瘙痒次数、瘙痒持续总时间及血清SOD、MDA水平的影响;建立组胺诱导的小鼠皮肤血管渗透性升高模型观察拟黑多刺蚁活性组分对血管通透性的影响,并以抗炎、镇痛实验观察拟黑多刺蚁活性组分的止痒作用及其可能机制。结果:拟黑多刺蚁活性组分可显著缩短瘙痒小鼠瘙痒总时间,增强瘙痒小鼠血清SOD活性;显著减少炎症部位渗出液的光密度;显著抑制二甲苯引起小鼠耳廓肿胀;显著提高小鼠热板痛阈值;显著减少醋酸引起小鼠扭体反应次数。结论:拟黑多刺蚁活性组分对右旋糖酐致痒及磷酸组胺的血管通透性增高具有显著的缓解作用,并具有抗炎、镇痛作用,其机理可能与升高机体的SOD活性有关。
引文
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[15] Gutierrez-Mecinas M,Bell AM,Marin A,et al.Preprotachykinin A is expressed by a distinct population of excitatory neurons in the mouse superficial spinal dorsal horn including cells that respond to noxious and pruritic stimuli[J].Pain,2017,158(3):440-456.
[2] 韦桂宁,苏启表,何飞,等.拟黑多刺蚁乙醇提取物中降低小鼠血清尿酸水平活性部位的筛选与化学成分分析[J].中国药理学与毒理学杂志,2013,27(4):673-677.
[3] 韦桂宁,曾宪彪,牙启康,等.拟黑多翅蚁抗痛风作用研究[J].中国实验方剂学杂志,2012,18(20):205-207.
[4] 韦桂宁,楚世峰,苏华,等.拟黑多刺蚁提取物抗抑郁作用研究[J].中国药理学通报,2015,31(9):1280-1286.
[5] 李仪奎.中药药理实验方法学[M].上海:上海科学技术出版社,2006.
[6] 黄国鑫,任薇,武文精,等.制蟒蛇油抗炎抗过敏止痒作用的实验研究[J].新中医,2010,42(12):119-121.
[7] Diehl C,Lipozenci D.The basis of topical superoxide dismutase antipruritic activity[J].Dermatovenerol Croat,2009,17(1):25-39.
[8] Guarneri F,Minciullo PL,Mannucci C,et al.IL-31 and IL-33 circulating levels in allergic contact dermatitis[J].Eur Ann Allergy Clin Immunol,2015,47(5):156-158.
[9] Foroutan A,Haddadi NS,Ostadhadi S,et al.Chloroquine-induced scratching is mediated by NO/cGMP pathway in mice[J].Pharmacol Biochem Behav,2015,134:79-84.
[10] Lewis KE,Holdren MS,Maurer MF,et al.Interleukin(IL)31 induces in cynomolgus monkeys a rapid and intense itch response that can be inhibited by an IL-31 neutralizing antibody[J].J Eur Acad Dermatol Venereol,2017,31(1):142-150.
[11] Pereira PJ,Machado GD,Danesi GM,et al.GRPR/PI3Kγ:Partners in Central Transmission of Itch[J].J Neurosci,2015,35(49):16272-16281.
[12] Choi JW,Lee J,Park YI.7,8-Dihydroxyflavone attenuates TNF-α-induced skin aging in Hs68 human dermal fibroblast cells via down-regulation of the MAPKs/Akt signaling pathways[J].Biomed Pharmacother,2017,95:1580-1587.
[13] Soko?owska-Wojdy?o M,Gleń J,Zabotna M,et al.The frequencies of haplotypes defined by three polymorphisms of the IL-31 gene:-1066,-2057,and IVS2+12 in Polish patients with atopic dermatitis[J].Int J Dermatol,2015,54(1):62-67.
[14] Lou H,Lu J,Choi EB,et al.Expression of IL-22 in the Skin Causes Th2-Biased Immunity,Epidermal Barrier Dysfunction,and Pruritus via Stimulating Epithelial Th2 Cytokines and the GRP Pathway[J].J Immunol,2017,198(7):2543-2555.
[15] Gutierrez-Mecinas M,Bell AM,Marin A,et al.Preprotachykinin A is expressed by a distinct population of excitatory neurons in the mouse superficial spinal dorsal horn including cells that respond to noxious and pruritic stimuli[J].Pain,2017,158(3):440-456.