摘要
目的观察X线全身照射对2型糖尿病模型KKAy小鼠的造血免疫系统功能的损伤作用,并与对照C57小鼠进行比较。方法 KKAy小鼠,分为对照组和照射组,照射组小鼠经X线全身照射,剂量4 Gy,C57小鼠作为对照。照射后15 d检测小鼠的外周血常规,流式细胞术检测骨髓中造血祖细胞、造血干细胞和长期造血干细胞的比例,脾中B细胞和T细胞的比例,胸腺中CD4CD8双阳性T细胞、CD4单阳性T细胞和CD8单阳性T细胞的比例。通过粒细胞集落形成能力实验评价小鼠造血祖细胞的功能。结果照射前KKAy小鼠的HSC和LT-HSC的比例低于C57小鼠。4 Gy全身照射后,KKAy小鼠的外周血WBC、RBC、PLT、HGB和LYM%分别下降了68. 42%、12. 17%、8. 78%、30. 12%、70. 84%;骨髓中HPC、HSC和LT-HSC的比例分别下降了34. 02%、29. 49%、35. 74%;脾B细胞和T细胞的比例分别下降了57. 85%、58. 81%;胸腺CD4CD8双阳性细胞的比例下降了51. 70%。KKAy小鼠的骨髓HSC、LT-HSC、外周血RBC和HGB的降低幅度显著低于C57小鼠。结论 4 Gy全身照射损伤KKAy小鼠的造血免疫系统功能,KKAy小鼠可能比C57小鼠表现出对电离辐射较强的耐受性。
Objective To explore the damaging effect of X-ray whole body irradiation on hematopoiesis and immune functions in KKAy mice with type 2 diabetes mellitus. Methods KKAy mice were exposed to 4 Gy total body irradiation( TBI),and compared with C57 BL/6 J mice. All mice were euthanized for routine analysis of peripheral blood,hematopoietic progenitor cells( HPC),hematopoietic stem cells( HSC),and long-term hematopoietic stem cells( LTHSC) frequencies and the percentages of splenic lymphocytes and thymic lymphocytes. The function of HPCs was measured by colony-forming unit-granulocyte and macrophage( CFU-GM) assay. Results The frequency of HSCs and LT-HSCs in KKAy mice was lower than that in C57 mice. After 4 Gy whole body irradiation,the percentages of WBCs,RBCs,PLTs,HG B and LYM% in peripheral blood of KKAy mice were decreased by 68. 42%,12. 17%,8. 78%,30. 12%,and70. 84%,respectively. The percentages of HPCs,HSCs and LT-HSCs in bone marrow were decreased by 34. 02%,29. 49%,35. 74%,respectively. The proportions of splenic B and T cells were decreased by 57. 85% and 58. 81%,respectively. The proportion of thymic CD4+/CD8+cells was decreased by 51. 7%. The HPC function( CFU-GM) was also impaired. The decrease levels of bone marrow HSC,LT-HSC,peripheral blood RBC and HGB in KKAy mice were significantly lower than those in C57 mice.Conclusions Whole body irradiation by 4 Gy X-rays impairs the hematopoiesis and immune functions,and KKAy mice might show a greater tolerance to ionizing radiation than C57 mice.
引文
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