稻曲病侵染规律研究进展与展望
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摘要
水稻稻曲病(Rice false smut,RFS)发生情况日趋严重,已经上升为水稻主要病害之一。目前对稻曲病的研究已经取得一定的进展,但对其病菌侵染方式和侵染机理的了解还有局限性。通过激光共聚焦和透射电镜等方法已经基本明确了稻曲病菌在颖花内的侵染过程,然而稻曲病是否具有系统侵染、种子带菌以及幼苗根部侵染对病害发生的影响程度仍有待明确。对稻曲病菌研究中存在的亟待解决的问题进行了探讨分析,同时也对稻曲病主要的防控手段和病原监测方法进行了总结。
Rice false smut has become one of the most severe rice disease in China. At present, some progress has been made in the study of rice false smut. There are still some puzzles about infection site and infection mechanism in Villosiclava virens. Laser confocal microscopy and transmission electron microscopy(TEM) have helped to provide the specific description of infection process of rice false smut in spikelet. However, there were no molecular evidence about the contribution of systemic infection, seedling and seed infection in life cycle. The problems existing in the research of rice false smut were discussed and analyzed. At the same time, the main methods of prevention and control of rice false smut and the methods of pathogen surveillance were summarized.
Rice false smut has become one of the most severe rice disease in China. At present, some progress has been made in the study of rice false smut. There are still some puzzles about infection site and infection mechanism in Villosiclava virens. Laser confocal microscopy and transmission electron microscopy(TEM) have helped to provide the specific description of infection process of rice false smut in spikelet. However, there were no molecular evidence about the contribution of systemic infection, seedling and seed infection in life cycle. The problems existing in the research of rice false smut were discussed and analyzed. At the same time, the main methods of prevention and control of rice false smut and the methods of pathogen surveillance were summarized.
引文
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[3]YONG M,DENG Q,FAN L,et al.The role of Ustilaginoidea virens sclerotia in increasing incidence of rice false smut disease in the subtropical zone in China[J].European journal of plant pathology,2018,150(3):669-677.
[4]MENG J,SUN W,MAO Z,et al.Main ustilaginoidins and their distribution in rice false smut balls[J].Toxins,2015,7(10):4023.
[5]WANG X H,WANG J,LAI D,et al.Ustiloxin G,a new cyclopeptide mycotoxin from rice false smut balls[J].Toxins,2017,9(2):54.
[6]张舒,张求东,罗汉刚,等.水稻稻曲病的药效试验和防治适期的选定[J].华中农业大学学报,2007,26(2):178-181.
[7]张爱芳,陈雨,王春林,等.安徽省水稻区试品种(系)对稻曲病的抗性鉴定及评价[J].植物保护,2013,39(1):151-153.
[8]兰波,杨迎青,孙强,等.江西晚稻“籼改粳”候选品种对稻曲病的抗性评价[J].江西农业大学学报,2016,38(6):1029-1035.
[9]HAN Y,ZHANG K,YANG J,et al.Differential expression profiling of the early response to Ustilaginoidea virens between false smut resistant and susceptible rice varieties[J].BMC Genomics,2015,16(1):955.
[10]ANDARGIE M,LI L,FENG A,et al.Mapping of the quantitative trait locus(QTL)conferring resistance to rice false smut disease[J].Current plant biology,2018,15:38-43.
[11]ZHOU Y L,XIE X W,ZHANG F,et al.Detection of quantitative resistance loci associated with resistance to rice false smut(Ustilaginoidea virens)using introgression lines[J].Plant pathology,2014,63(2):365-372.
[12]王文斌,张荣胜,罗楚平,等.中国主要稻区稻曲病菌的生物学特性及群体遗传多样性[J].中国农业科学,2014,47(14):2762-2773.
[13]WANG F,ZHANG S,LIU M-G,et al.Genetic diversity analysis reveals that geographical environment plays a more important role than rice cultivar in Villosiclava virens population selection[J].Applied and environmental microbiology,2014,80(9):2811-2820.
[14]徐晗,白元俊,缪建锟,等.不同储存时期稻曲病菌分离方法的比较[J].吉林农业大学学报,2017,39(5):524-526.
[15]胡贤锋,李荣玉,王健,等.水稻稻曲病菌的分离鉴定及室内毒力测定[J].福建农业学报,2017,32(6):634-638.
[16]王丽梅,陈莉,丁克坚,等.水稻稻曲病菌生长与侵染影响因子的研究[J].安徽农业大学学报,2012,39(3):470-473.
[17]CHEN Y,YAO J,LI Y F,et al.Simple and rapid detection of rice false smut pathogen Ustilaginoidea virens in rice seeds[J].Phytoparasitica,2014,42(3):371-375.
[18]ZHOU Y L,IZUMITSU K,SONODA R,et al.PCR-based Specific Detection of Ustilaginoidea virens and Ephelis japonica[J].Journal of phytopathology,2003,151(9):513-518.
[19]YANG X,AL ATTALA M N,ZHANG Y,et al.Rapid detection of Ustilaginoidea virens from rice using loop-mediated isothermal amplification assay[J].Plant disease,2018,102(9):1741-1747.
[20]杜毅,王疏,褚茗莉,等.利用稻曲病菌无性孢子接种研究初报[J].辽宁农业科学,1992(1):48-49.
[21]ASHIZAWA T,TAKAHASHI M,MORIWAKI J,et al.A refined inoculation method to evaluate false smut resistance in rice[J].Journal of general plant pathology,2011,77(1):10-16.
[22]张君成,陈志谊,张炳欣,等.稻曲病的接种技术研究[J].植物病理学报,2004,34(5):463-467.
[23]ASHIZAWA T,TAKAHASHI M,ARAI M,et al.Rice false smut pathogen,Ustilaginoidea virens,invades through small gap at the apex of a rice spikelet before heading[J].Journal of general plant pathology,2012,78(4):255-259.
[24]TANG J,ZHENG L,JIA Q,et al.PCR markers derived from comparative genomics for detection and identification of the rice pathogen Ustilaginoidea virens in plant tissues[J].Plant disease,2017,101(8):1515-1521.
[25]SONG J H,WEI W,LV B,et al.Rice false smut fungus hijacks the rice nutrients supply by blocking and mimicking the fertilization of rice ovary[J].Environmental microbiology,2016,18(11):3840-3849.
[26]伏荣桃,王剑,陈诚,等.水稻生育期空气中稻曲病菌孢子释放动态及影响因素分析[J].南方农业学报,2017,48(11):1994-1998.
[27]俞咪娜,陈志谊,于俊杰,等.来源于同一穗不同稻曲球的稻曲病菌的致病性及遗传多样性[J].植物病理学报,2013,43(6):561-573.
[28]张舒,张求东,王利兵,等.稻曲病Usitilaginoidea vriens厚垣孢子侵染水稻方式研究[A].中国植物病理学会第八届青年学术研讨会论文集[C].北京:中国植物病理学会,2007.
[29]刘见平,唐涛,张松柏,等.稻曲病初侵染源及病菌侵染适期初步研究[J].杂交水稻,2009,24(1):74-77.
[30]陈永坚,肖炎农,赵永静.稻曲病种子传病的研究[J].湖北植保,1994(3):2-3.
[31]吕博,杨丹,贾切,等.基于PCR技术的稻曲病菌侵染特性研究[A].中国植物病理学会2009年学术年会论文集[C].北京:中国植物病理学会,2009.
[32]郑大伟,何黎平,孟春梅,等.稻曲病菌在水稻萌发期侵染的细胞学证据[A].中国植物病理学会2009年学术年会论文集[C].北京:中国植物病理学会,2009.
[33]陈丽,胡东维,陈美军,等.稻曲球及稻曲病菌菌落微结构的SEM观察[J].菌物学报,2007,26(1):89-96.
[34]FAN J,GUO X Y,LI L,et al.Infection of Ustilaginoidea virens intercepts rice seed formation but activates grain-filling-related genes[J].Journal of integrative plant biology,2015,57(6):577-590.
[35]TANG Y X,JIN J,HU D W,et al.Elucidation of the infection process of Ustilaginoidea virens(teleomorph:Villosiclava virens)in rice spikelets[J].Plant pathology,2013,62(1):1-8.
[36]YIN W,CUI P,WEI W,et al.Genome-wide identification and analysis of the basic leucine zipper(bZIP)transcription factor gene family in Ustilaginoidea virens[J].Genome,2017,60(12):1051-1059.
[37]ZHANG Y,ZHANG K,FANG A,et al.Specific adaptation of Ustilaginoidea virens in occupying host florets revealed by comparative and functional genomics[J].Nature communications,2014,5:3849.
[38]FANG A,HAN Y,ZHANG N,et al.Identification and characterization of plant cell death-inducing secreted proteins from Ustilaginoidea virens[J].Molecular plant-microbe interactions,2016,29(5):405-416.
[39]ZHENG D,WANG Y,HAN Y,et al.UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens[J].Scientific reports,2016,6:24824.
[40]LV B,ZHENG L,LIU H,et al.Use of random T-DNA mutagenesis in identification of gene UvPRO1,a regulator of conidiation,stress response,and virulence in Ustilaginoidea virens[J].Frontiers in microbiology,2016,7:2086.
[41]LIANG Y,HAN Y,WANG C,et al.Targeted deletion of the USTA and UvSLT2 genes efficiently in Ustilaginoidea virens with the CRISPR-Cas9 system[J].Frontiers in plant science,2018,9:699.
[42]龚林根,张念环,张景飞,等.江苏常熟地区稻曲病发生规律及防治技术[J].江苏农业科学,2001(6):30-32.
[2]GUO X,LI Y,FAN J,et al.Progress in the study of false smut disease in rice[J].Journal of agricultural science&technology,2012,11:1211-1217.
[3]YONG M,DENG Q,FAN L,et al.The role of Ustilaginoidea virens sclerotia in increasing incidence of rice false smut disease in the subtropical zone in China[J].European journal of plant pathology,2018,150(3):669-677.
[4]MENG J,SUN W,MAO Z,et al.Main ustilaginoidins and their distribution in rice false smut balls[J].Toxins,2015,7(10):4023.
[5]WANG X H,WANG J,LAI D,et al.Ustiloxin G,a new cyclopeptide mycotoxin from rice false smut balls[J].Toxins,2017,9(2):54.
[6]张舒,张求东,罗汉刚,等.水稻稻曲病的药效试验和防治适期的选定[J].华中农业大学学报,2007,26(2):178-181.
[7]张爱芳,陈雨,王春林,等.安徽省水稻区试品种(系)对稻曲病的抗性鉴定及评价[J].植物保护,2013,39(1):151-153.
[8]兰波,杨迎青,孙强,等.江西晚稻“籼改粳”候选品种对稻曲病的抗性评价[J].江西农业大学学报,2016,38(6):1029-1035.
[9]HAN Y,ZHANG K,YANG J,et al.Differential expression profiling of the early response to Ustilaginoidea virens between false smut resistant and susceptible rice varieties[J].BMC Genomics,2015,16(1):955.
[10]ANDARGIE M,LI L,FENG A,et al.Mapping of the quantitative trait locus(QTL)conferring resistance to rice false smut disease[J].Current plant biology,2018,15:38-43.
[11]ZHOU Y L,XIE X W,ZHANG F,et al.Detection of quantitative resistance loci associated with resistance to rice false smut(Ustilaginoidea virens)using introgression lines[J].Plant pathology,2014,63(2):365-372.
[12]王文斌,张荣胜,罗楚平,等.中国主要稻区稻曲病菌的生物学特性及群体遗传多样性[J].中国农业科学,2014,47(14):2762-2773.
[13]WANG F,ZHANG S,LIU M-G,et al.Genetic diversity analysis reveals that geographical environment plays a more important role than rice cultivar in Villosiclava virens population selection[J].Applied and environmental microbiology,2014,80(9):2811-2820.
[14]徐晗,白元俊,缪建锟,等.不同储存时期稻曲病菌分离方法的比较[J].吉林农业大学学报,2017,39(5):524-526.
[15]胡贤锋,李荣玉,王健,等.水稻稻曲病菌的分离鉴定及室内毒力测定[J].福建农业学报,2017,32(6):634-638.
[16]王丽梅,陈莉,丁克坚,等.水稻稻曲病菌生长与侵染影响因子的研究[J].安徽农业大学学报,2012,39(3):470-473.
[17]CHEN Y,YAO J,LI Y F,et al.Simple and rapid detection of rice false smut pathogen Ustilaginoidea virens in rice seeds[J].Phytoparasitica,2014,42(3):371-375.
[18]ZHOU Y L,IZUMITSU K,SONODA R,et al.PCR-based Specific Detection of Ustilaginoidea virens and Ephelis japonica[J].Journal of phytopathology,2003,151(9):513-518.
[19]YANG X,AL ATTALA M N,ZHANG Y,et al.Rapid detection of Ustilaginoidea virens from rice using loop-mediated isothermal amplification assay[J].Plant disease,2018,102(9):1741-1747.
[20]杜毅,王疏,褚茗莉,等.利用稻曲病菌无性孢子接种研究初报[J].辽宁农业科学,1992(1):48-49.
[21]ASHIZAWA T,TAKAHASHI M,MORIWAKI J,et al.A refined inoculation method to evaluate false smut resistance in rice[J].Journal of general plant pathology,2011,77(1):10-16.
[22]张君成,陈志谊,张炳欣,等.稻曲病的接种技术研究[J].植物病理学报,2004,34(5):463-467.
[23]ASHIZAWA T,TAKAHASHI M,ARAI M,et al.Rice false smut pathogen,Ustilaginoidea virens,invades through small gap at the apex of a rice spikelet before heading[J].Journal of general plant pathology,2012,78(4):255-259.
[24]TANG J,ZHENG L,JIA Q,et al.PCR markers derived from comparative genomics for detection and identification of the rice pathogen Ustilaginoidea virens in plant tissues[J].Plant disease,2017,101(8):1515-1521.
[25]SONG J H,WEI W,LV B,et al.Rice false smut fungus hijacks the rice nutrients supply by blocking and mimicking the fertilization of rice ovary[J].Environmental microbiology,2016,18(11):3840-3849.
[26]伏荣桃,王剑,陈诚,等.水稻生育期空气中稻曲病菌孢子释放动态及影响因素分析[J].南方农业学报,2017,48(11):1994-1998.
[27]俞咪娜,陈志谊,于俊杰,等.来源于同一穗不同稻曲球的稻曲病菌的致病性及遗传多样性[J].植物病理学报,2013,43(6):561-573.
[28]张舒,张求东,王利兵,等.稻曲病Usitilaginoidea vriens厚垣孢子侵染水稻方式研究[A].中国植物病理学会第八届青年学术研讨会论文集[C].北京:中国植物病理学会,2007.
[29]刘见平,唐涛,张松柏,等.稻曲病初侵染源及病菌侵染适期初步研究[J].杂交水稻,2009,24(1):74-77.
[30]陈永坚,肖炎农,赵永静.稻曲病种子传病的研究[J].湖北植保,1994(3):2-3.
[31]吕博,杨丹,贾切,等.基于PCR技术的稻曲病菌侵染特性研究[A].中国植物病理学会2009年学术年会论文集[C].北京:中国植物病理学会,2009.
[32]郑大伟,何黎平,孟春梅,等.稻曲病菌在水稻萌发期侵染的细胞学证据[A].中国植物病理学会2009年学术年会论文集[C].北京:中国植物病理学会,2009.
[33]陈丽,胡东维,陈美军,等.稻曲球及稻曲病菌菌落微结构的SEM观察[J].菌物学报,2007,26(1):89-96.
[34]FAN J,GUO X Y,LI L,et al.Infection of Ustilaginoidea virens intercepts rice seed formation but activates grain-filling-related genes[J].Journal of integrative plant biology,2015,57(6):577-590.
[35]TANG Y X,JIN J,HU D W,et al.Elucidation of the infection process of Ustilaginoidea virens(teleomorph:Villosiclava virens)in rice spikelets[J].Plant pathology,2013,62(1):1-8.
[36]YIN W,CUI P,WEI W,et al.Genome-wide identification and analysis of the basic leucine zipper(bZIP)transcription factor gene family in Ustilaginoidea virens[J].Genome,2017,60(12):1051-1059.
[37]ZHANG Y,ZHANG K,FANG A,et al.Specific adaptation of Ustilaginoidea virens in occupying host florets revealed by comparative and functional genomics[J].Nature communications,2014,5:3849.
[38]FANG A,HAN Y,ZHANG N,et al.Identification and characterization of plant cell death-inducing secreted proteins from Ustilaginoidea virens[J].Molecular plant-microbe interactions,2016,29(5):405-416.
[39]ZHENG D,WANG Y,HAN Y,et al.UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens[J].Scientific reports,2016,6:24824.
[40]LV B,ZHENG L,LIU H,et al.Use of random T-DNA mutagenesis in identification of gene UvPRO1,a regulator of conidiation,stress response,and virulence in Ustilaginoidea virens[J].Frontiers in microbiology,2016,7:2086.
[41]LIANG Y,HAN Y,WANG C,et al.Targeted deletion of the USTA and UvSLT2 genes efficiently in Ustilaginoidea virens with the CRISPR-Cas9 system[J].Frontiers in plant science,2018,9:699.
[42]龚林根,张念环,张景飞,等.江苏常熟地区稻曲病发生规律及防治技术[J].江苏农业科学,2001(6):30-32.