单倍体育种技术研究进展
|
摘要
单倍体指含有单套染色体的个体,由单倍体通过染色体加倍可得到纯合的二倍体,称为双单倍体。单倍体育种已经被越来越多的研究机构和商业育种公司运用到遗传育种研究和实践当中。文章综述了单倍体育种中的重要技术环节及其在育种中应用的最新进展。
A haploid organism is an individual containing a single set of chromosomes. A double haploid is a homozygous diploid of haploids by double chromosomes. Haploid breeding has been widely used in the research of genetic breeding by research institutions and commercial breeding companies worldwide. In this paper, the important technical aspects in haploid breeding and the latest progress in the breeding of haploid plants are reviewed.
A haploid organism is an individual containing a single set of chromosomes. A double haploid is a homozygous diploid of haploids by double chromosomes. Haploid breeding has been widely used in the research of genetic breeding by research institutions and commercial breeding companies worldwide. In this paper, the important technical aspects in haploid breeding and the latest progress in the breeding of haploid plants are reviewed.
引文
[1]THOMPSON K F.Cytoplasmic male-sterility in oil-seed rape[J].Heredity,1972,29(1):253-257.
[2]CHASE S S.Efficient methods of developing and improving inbred lines.The monoploid method of developing inbred lines[C]∥Report of the 6th Hybrid Corn Industry Research Conference.Chicago,IL:American Seed Trade Association,Hybrid Corn Division,1951:29-34.
[3]MAHESHWARI S C,GUHA S.In vitro production of embryos from anthers of Datura[J].Nature,1964,204(4957):497.
[4]MALUSZYNSKI M,KASHA K J,FORSTER B P,et al.Doubled Haploid Production in Crop Plants[M].Netherlands:Springer,2003:309-336.
[5]DUNWELL J M.Haploids in flowering plants:origins and exploitation[J].Plant Biotechnology Journal,2010,8(4):377-424.
[6]MUROVEC J,BOHANEC B.Haploids and doubled haploids in plant breeding[M].Croatia:Intech,2012:87-106.
[7]LICHTER R.Induction of haploid plants from isolated pollen of Brassica napus[J].Zeitschrift Für Pflanzenphysiol ogie,1982,105(5):427-434.
[8]KOHLER F,WENZEL G.Regeneration of isolated barley microspores in conditioned media and trials to characterize the responsible factor[J].Journal of Plant Physiology,1985,121(2):181-191.
[9]CHO M S,ZAPATA F J.Callus formation and plant regeneration on isolated pollen culture of rice(Oryza sativa L.cv.Taipei309)[J].Plant Science,1988,58:239-244.
[10]SATO T,NISHIO T,HIRAI M.Plant regeneration from isolated microspore cultures of Chinese cabbage(Brassica campestris spp.pekinensis)[J].Plant Cell Reports,1989,8(8):486-488.
[11]LICHTER R.Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species[J].Plant Breeding,1989,103(2):119-123.
[12]SAN NOEUM,L H.Haploides of Hordeum vulgare L.from in vitro culture of unfertilized ovaries[J].Annales de Amelioration des Plantes,1976,26:751-754.
[13]XU Y.Molecular plant breeding[M].Wallingford,UK:Centre for Agriculture and Biosciences International,2010:116.
[14]PALMER C E D,KELLER W A.Haploids in crop improvement II[M].Berlin Heidelberg:Springer,2005:3-9.
[15]KASHA K J,KAO K N.High frequency haploid production in barley(Hordeum vulgare L.)[J].Nature,1970,225(5235):874.
[16]ZENKTELER M,NITZSCHE W.Wide hybridization experiments in cereals[J].Tag.theoretical&Applied Genetics theoretische Und Angewandte Genetik,1984,68(4):311-315.
[17]LAURIE D A,BENNETT M D.Wheat×maize hybridization[J].Genome,1986,28(2):313-316.
[18]LAURIE D A,REYMONDIE S.High frequencies of fertilization and haploid seedling production in crosses between commercial hexaploid wheat varieties and maize[J].Plant Breeding,1991,106(3):182-189.
[19]TURCOTTE E L,FEASTER C V.Haploids:highfrequency production from single-embryo seeds in a line of pima cotton[J].Science,1963,140(3574):1407-1408.
[20]ZHANG J F,STEWART J M.Semigamy gene is associated with chlorophyll reduction in cotton[J].Crop Science,2004,44(6):2054-2062.
[21]COE E H.A line of maize with high haploid frequency[J].American Naturalist,1959,93(873):381-382.
[22]SARKAR K R,PANDEY A,CAYEN P,et al.Stabilization of high haploid inducer lines[J].Maize Genetics Cooperat ion Newsletter,1994,68:64-65.
[23]SHATSKAYA O A,ZABIROVA E R,SHCHERBAK V S,et al.Mass induction of maternal haploids in corn[J].Maize Genetics Cooperation Newsletter,1994,68:51.
[24]MELCHINGER A E,LONGIN C F H,UTZ H F,et al.Hybrid maize breeding with doubled haploid lines:quantitative genetic and selection theory for optimum allocation of resources[C]∥Proceedings of the Forty First Annual Illinois Corn Breeders'School.Illinois,USA:Urbana-Champaign,2005:8-21.
[25]R魻BER F K,GORDILLO G A,GEIGER H H.In vivo haploid induction in maize-Performance of new inducers and significance of doubled haploid lines in hybrid breeding[J].Maydica,2005,50(3):275-283.
[26]SWAMINATHAN M S,SINGH M P.X-ray induced somatic haploidy in watermelon[J].Current Science,1958,2:63-64.
[27]H魧NTZSCHEL K R,WEBER G.Blockage of mitosis in maize root tips using colchicine-alternatives[J].Protoplasma,2010,241(1-4):99-104.
[28]KATO A,GEIGER H H.Chromosome doubling of haploid maize seedlings using nitrous oxide gas at the flower primordial stage[J].Plant Breeding,2002,121(5):370-377.
[29]陈绍江.玉米单倍体育种技术[M].北京:中国农业大学出版社,2009.
[30]WU P,REN J,LI L,et al.Early spontaneous diploidization of maternal maize haploids generated by in vivo haploid induction[J].Euphytica,2014,200(1):127-138.
[31]ZHANG L,ZHANG L,LUO J,et al.Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process[J].遗传学报(英文版),2011,38(2):89-94.
[32]CHALYK S T.Properties of maternal haploid maize plants and potential application to maize breeding[J].Euphytica,1994,79(1-2):13-18.
[33]CHEN Z Z,SNYDER S,FAN Z G,et al.Efficient production of doubled haploid plants through chromosome doubling of isolated microspores in Brassica napus[J].Plant Breeding,1994,113(3):217-221.
[34]袁素霞,刘玉梅,方智远,等.甘蓝类蔬菜小孢子再生植株染色体倍性与气孔保卫细胞叶绿体数的相关性[J].中国农业科学,2009,42(1):189-197.
[35]MALUSZYNSKI M,KASHA K J,FORSTER B P,et al.Doubled haploid production in crop plants:a manual[M].Dordrecht:Kluwer Academic Publishers,2003:391-395.
[36]WEBER S,WILFRIED L,FRIEDT W.Efficient doubled haploid production in Brassica napus via microspore colchicine treatment in vitro and ploidy determination by flow cytometry[J].GCIRC-Bull,2004:1-6.
[37]DOLEZEL J,GREILHUBER J,SUDAJ.Flow Cytometry with plant cells:analysis of genes,chromosomes and genomes[M].Weinheim:Wiley-VCH Verlag GmbH&Co.KGaA,2007:41-65.
[38]OCHATT S.Flow cytometry in plant breeding[J].Cytometry Part A the Journal of the International Society for Analytical Cytology,2008,73(7):581-598.
[39]MELCHINGER A E,SCHIPPRACK W,UTZ H F,et al.In vivo haploid induction in maize:identification of haploid seeds by their oil content[J].Crop Science,2014,54(4):1497-1504.
[40]TANG F,TAO Y,ZHAO T,et al.In vitro,production of haploid and doubled haploid plants from pollinated ovaries of maize(Zea mays)[J].Plant Cell Tissue&Organ Culture,2006,84(2):233-237.
[41]MURANTY H,SOURDILLE P,BERNARD S,et al.Genetic characterization of spontaneous diploid androgenetic wheat and triticale plants[J].Plant Breeding,2002,121(6):470-474.
[42]H魻FER M,GOMEZ A,AGUIRIANO E,et al.Analysis of simple sequence repeat markers in homozygous lines of apple[J].Plant Breeding,2002,121(2):159-162.
[43]DIAO W P,JIA Y Y,SONG H,et al.Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenetants using SSR markers[J].Scientia Horticulturae,2009,119(3):246-251.
[44]MALIK A A,LI C,ZHANG S X,et al.Efficiency of SSRmarkers for determining the origin of melon plantlets derived through unfertilized ovary culture[J].Horticultural Science,2011,38(1):27-34.
[45]HOFINGER B J,HUYNH O A,JANKOWICZ-CIESLAKJ,et al.Validation of doubled haploid plants by enzymaticˇmismatch cleavage[J].Plant Methods,2013,9(1):43.
[46]SEITZ G.The use of doubled haploids in corn breeding[C]∥Proceedings of the Forty First Annual Illinois Corn Breeders'School.Illinois,USA:Urbana-Champaign,2005:1-7.
[47]DWIVEDI S L,CROUCH J H,MACKILL D J,et al.The molecularization of public sector crop breeding:progress,problems,and prospects[J].Advances in Agronomy,2007,95:163-318.
[48]BELICUAS P R,GUIMARAES C T,PAIVA L V,et al.Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids[J].Euphytica,2007,156(1-2):95-102.
[49]BAKHTIAR F,AFSHARI F,NAJAFIAN G,et al.Backcross-breeding and doubled-haploid facilitated introgression of stripe rust resistance in bread wheat[J].Archiv Pflanzenschutz,2014,47(14):1675-1685.
[50]DE ARAUJO L G,PRABHU A S,PEREIRA P A A,et al.Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population[J].Crop Breeding&Applied Biotechnology,2010,10(1):23-31.
[51]LV H H,YANG L M,KANG J G,et al.Development of InDel markers linked to fusarium wilt resistance in cabbage[J].Molecular Breeding,2013,32(4):961-967.
[52]LV H H,WANG Q B,YANG L M,et al.Breeding of cabbage(Brassica oleracea,L.var.capitata)with fusarium wilt resistance based on microspore culture and markerassisted selection[J].Euphytica,2014,200(3):465-473.
[53]KEBEDE A Z,DHILLON B S,SCHIPPRACK W,et al.Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize[J].Euphytica,2011,180(2):219-226.
[54]MELCHINGER A E,SCHIPPRACK W,W譈RSCHUM T,et al.Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize[J].Scientific Reports,2013,3(3):2129.
[2]CHASE S S.Efficient methods of developing and improving inbred lines.The monoploid method of developing inbred lines[C]∥Report of the 6th Hybrid Corn Industry Research Conference.Chicago,IL:American Seed Trade Association,Hybrid Corn Division,1951:29-34.
[3]MAHESHWARI S C,GUHA S.In vitro production of embryos from anthers of Datura[J].Nature,1964,204(4957):497.
[4]MALUSZYNSKI M,KASHA K J,FORSTER B P,et al.Doubled Haploid Production in Crop Plants[M].Netherlands:Springer,2003:309-336.
[5]DUNWELL J M.Haploids in flowering plants:origins and exploitation[J].Plant Biotechnology Journal,2010,8(4):377-424.
[6]MUROVEC J,BOHANEC B.Haploids and doubled haploids in plant breeding[M].Croatia:Intech,2012:87-106.
[7]LICHTER R.Induction of haploid plants from isolated pollen of Brassica napus[J].Zeitschrift Für Pflanzenphysiol ogie,1982,105(5):427-434.
[8]KOHLER F,WENZEL G.Regeneration of isolated barley microspores in conditioned media and trials to characterize the responsible factor[J].Journal of Plant Physiology,1985,121(2):181-191.
[9]CHO M S,ZAPATA F J.Callus formation and plant regeneration on isolated pollen culture of rice(Oryza sativa L.cv.Taipei309)[J].Plant Science,1988,58:239-244.
[10]SATO T,NISHIO T,HIRAI M.Plant regeneration from isolated microspore cultures of Chinese cabbage(Brassica campestris spp.pekinensis)[J].Plant Cell Reports,1989,8(8):486-488.
[11]LICHTER R.Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species[J].Plant Breeding,1989,103(2):119-123.
[12]SAN NOEUM,L H.Haploides of Hordeum vulgare L.from in vitro culture of unfertilized ovaries[J].Annales de Amelioration des Plantes,1976,26:751-754.
[13]XU Y.Molecular plant breeding[M].Wallingford,UK:Centre for Agriculture and Biosciences International,2010:116.
[14]PALMER C E D,KELLER W A.Haploids in crop improvement II[M].Berlin Heidelberg:Springer,2005:3-9.
[15]KASHA K J,KAO K N.High frequency haploid production in barley(Hordeum vulgare L.)[J].Nature,1970,225(5235):874.
[16]ZENKTELER M,NITZSCHE W.Wide hybridization experiments in cereals[J].Tag.theoretical&Applied Genetics theoretische Und Angewandte Genetik,1984,68(4):311-315.
[17]LAURIE D A,BENNETT M D.Wheat×maize hybridization[J].Genome,1986,28(2):313-316.
[18]LAURIE D A,REYMONDIE S.High frequencies of fertilization and haploid seedling production in crosses between commercial hexaploid wheat varieties and maize[J].Plant Breeding,1991,106(3):182-189.
[19]TURCOTTE E L,FEASTER C V.Haploids:highfrequency production from single-embryo seeds in a line of pima cotton[J].Science,1963,140(3574):1407-1408.
[20]ZHANG J F,STEWART J M.Semigamy gene is associated with chlorophyll reduction in cotton[J].Crop Science,2004,44(6):2054-2062.
[21]COE E H.A line of maize with high haploid frequency[J].American Naturalist,1959,93(873):381-382.
[22]SARKAR K R,PANDEY A,CAYEN P,et al.Stabilization of high haploid inducer lines[J].Maize Genetics Cooperat ion Newsletter,1994,68:64-65.
[23]SHATSKAYA O A,ZABIROVA E R,SHCHERBAK V S,et al.Mass induction of maternal haploids in corn[J].Maize Genetics Cooperation Newsletter,1994,68:51.
[24]MELCHINGER A E,LONGIN C F H,UTZ H F,et al.Hybrid maize breeding with doubled haploid lines:quantitative genetic and selection theory for optimum allocation of resources[C]∥Proceedings of the Forty First Annual Illinois Corn Breeders'School.Illinois,USA:Urbana-Champaign,2005:8-21.
[25]R魻BER F K,GORDILLO G A,GEIGER H H.In vivo haploid induction in maize-Performance of new inducers and significance of doubled haploid lines in hybrid breeding[J].Maydica,2005,50(3):275-283.
[26]SWAMINATHAN M S,SINGH M P.X-ray induced somatic haploidy in watermelon[J].Current Science,1958,2:63-64.
[27]H魧NTZSCHEL K R,WEBER G.Blockage of mitosis in maize root tips using colchicine-alternatives[J].Protoplasma,2010,241(1-4):99-104.
[28]KATO A,GEIGER H H.Chromosome doubling of haploid maize seedlings using nitrous oxide gas at the flower primordial stage[J].Plant Breeding,2002,121(5):370-377.
[29]陈绍江.玉米单倍体育种技术[M].北京:中国农业大学出版社,2009.
[30]WU P,REN J,LI L,et al.Early spontaneous diploidization of maternal maize haploids generated by in vivo haploid induction[J].Euphytica,2014,200(1):127-138.
[31]ZHANG L,ZHANG L,LUO J,et al.Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process[J].遗传学报(英文版),2011,38(2):89-94.
[32]CHALYK S T.Properties of maternal haploid maize plants and potential application to maize breeding[J].Euphytica,1994,79(1-2):13-18.
[33]CHEN Z Z,SNYDER S,FAN Z G,et al.Efficient production of doubled haploid plants through chromosome doubling of isolated microspores in Brassica napus[J].Plant Breeding,1994,113(3):217-221.
[34]袁素霞,刘玉梅,方智远,等.甘蓝类蔬菜小孢子再生植株染色体倍性与气孔保卫细胞叶绿体数的相关性[J].中国农业科学,2009,42(1):189-197.
[35]MALUSZYNSKI M,KASHA K J,FORSTER B P,et al.Doubled haploid production in crop plants:a manual[M].Dordrecht:Kluwer Academic Publishers,2003:391-395.
[36]WEBER S,WILFRIED L,FRIEDT W.Efficient doubled haploid production in Brassica napus via microspore colchicine treatment in vitro and ploidy determination by flow cytometry[J].GCIRC-Bull,2004:1-6.
[37]DOLEZEL J,GREILHUBER J,SUDAJ.Flow Cytometry with plant cells:analysis of genes,chromosomes and genomes[M].Weinheim:Wiley-VCH Verlag GmbH&Co.KGaA,2007:41-65.
[38]OCHATT S.Flow cytometry in plant breeding[J].Cytometry Part A the Journal of the International Society for Analytical Cytology,2008,73(7):581-598.
[39]MELCHINGER A E,SCHIPPRACK W,UTZ H F,et al.In vivo haploid induction in maize:identification of haploid seeds by their oil content[J].Crop Science,2014,54(4):1497-1504.
[40]TANG F,TAO Y,ZHAO T,et al.In vitro,production of haploid and doubled haploid plants from pollinated ovaries of maize(Zea mays)[J].Plant Cell Tissue&Organ Culture,2006,84(2):233-237.
[41]MURANTY H,SOURDILLE P,BERNARD S,et al.Genetic characterization of spontaneous diploid androgenetic wheat and triticale plants[J].Plant Breeding,2002,121(6):470-474.
[42]H魻FER M,GOMEZ A,AGUIRIANO E,et al.Analysis of simple sequence repeat markers in homozygous lines of apple[J].Plant Breeding,2002,121(2):159-162.
[43]DIAO W P,JIA Y Y,SONG H,et al.Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenetants using SSR markers[J].Scientia Horticulturae,2009,119(3):246-251.
[44]MALIK A A,LI C,ZHANG S X,et al.Efficiency of SSRmarkers for determining the origin of melon plantlets derived through unfertilized ovary culture[J].Horticultural Science,2011,38(1):27-34.
[45]HOFINGER B J,HUYNH O A,JANKOWICZ-CIESLAKJ,et al.Validation of doubled haploid plants by enzymaticˇmismatch cleavage[J].Plant Methods,2013,9(1):43.
[46]SEITZ G.The use of doubled haploids in corn breeding[C]∥Proceedings of the Forty First Annual Illinois Corn Breeders'School.Illinois,USA:Urbana-Champaign,2005:1-7.
[47]DWIVEDI S L,CROUCH J H,MACKILL D J,et al.The molecularization of public sector crop breeding:progress,problems,and prospects[J].Advances in Agronomy,2007,95:163-318.
[48]BELICUAS P R,GUIMARAES C T,PAIVA L V,et al.Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids[J].Euphytica,2007,156(1-2):95-102.
[49]BAKHTIAR F,AFSHARI F,NAJAFIAN G,et al.Backcross-breeding and doubled-haploid facilitated introgression of stripe rust resistance in bread wheat[J].Archiv Pflanzenschutz,2014,47(14):1675-1685.
[50]DE ARAUJO L G,PRABHU A S,PEREIRA P A A,et al.Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population[J].Crop Breeding&Applied Biotechnology,2010,10(1):23-31.
[51]LV H H,YANG L M,KANG J G,et al.Development of InDel markers linked to fusarium wilt resistance in cabbage[J].Molecular Breeding,2013,32(4):961-967.
[52]LV H H,WANG Q B,YANG L M,et al.Breeding of cabbage(Brassica oleracea,L.var.capitata)with fusarium wilt resistance based on microspore culture and markerassisted selection[J].Euphytica,2014,200(3):465-473.
[53]KEBEDE A Z,DHILLON B S,SCHIPPRACK W,et al.Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize[J].Euphytica,2011,180(2):219-226.
[54]MELCHINGER A E,SCHIPPRACK W,W譈RSCHUM T,et al.Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize[J].Scientific Reports,2013,3(3):2129.