根区温度对辣椒幼苗生理特性的影响
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摘要
为探讨冬季日光温室土壤温度对作物水分吸收、保护酶、渗透调节物质以及质膜透性的影响,以辣椒品种长金、迅驰37-74和运驰37-82为研究试材,对20~22,14~16,10~12℃3个温度处理下辣椒幼苗茎叶和根系的含水量,叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,脯氨酸(Pro)、丙二醛(MDA)含量和渗透电解率质膜透性进行了测定。结果表明,随着根区温度的下降,辣椒幼苗含水量呈现下降趋势,其中10~12℃处理含水量最低;叶片SOD、POD和CAT活性表现为下降的趋势;Pro、MDA、超氧阴离子含量以及电解渗透率表现为逐渐升高的趋势。综上所述,10~12℃处理对辣椒幼苗生理生化特性和含水量影响程度明显高于处理20~22,14~16℃,以生理生化特性和含水量为衡量指标,根区温度14℃及以上时,辣椒植株可以保持正常的生长发育。
To probe into the sunlight greenhouse soil temperature in the winter stress on crop water content,protective enzymes,osmolyte and membrane permeability in pepper,we comparatively studied three treatment( 20-22,14- 16,10- 12 ℃),the water contents of seedling stems and roots,the activities of super oxede-dismutase( SOD),peroxidase( POD),catalase( CAT),proline( Pro),malondialdehyde( MDA) and electrolytic permeability contents were determined by using Changjin,Xunchi 37- 74 and Yunchi 37- 82 in pepper. The results showed that as the root zone temperature dropped the seedling water content appeared the tendency of decline,among water content was the lowest in 10- 12 ℃ treatment. The activities of SOD,POD and CAT in leaf appeared the trend of decline. The contents of Pro,MDA and electrolytic perme ability appeared the trend of rising. In a word,10- 12 ℃treatment on influencing degree of water content,physiological and biochemical characteristics in pepper seedlings was significantly higher than 20- 22,14- 16 ℃ treatments. Taking physiological and biochemical characteristics as measurement index,we concluded that pepper could still keep its normal growth and development when the root zone temperature over 14 ℃.
To probe into the sunlight greenhouse soil temperature in the winter stress on crop water content,protective enzymes,osmolyte and membrane permeability in pepper,we comparatively studied three treatment( 20-22,14- 16,10- 12 ℃),the water contents of seedling stems and roots,the activities of super oxede-dismutase( SOD),peroxidase( POD),catalase( CAT),proline( Pro),malondialdehyde( MDA) and electrolytic permeability contents were determined by using Changjin,Xunchi 37- 74 and Yunchi 37- 82 in pepper. The results showed that as the root zone temperature dropped the seedling water content appeared the tendency of decline,among water content was the lowest in 10- 12 ℃ treatment. The activities of SOD,POD and CAT in leaf appeared the trend of decline. The contents of Pro,MDA and electrolytic perme ability appeared the trend of rising. In a word,10- 12 ℃treatment on influencing degree of water content,physiological and biochemical characteristics in pepper seedlings was significantly higher than 20- 22,14- 16 ℃ treatments. Taking physiological and biochemical characteristics as measurement index,we concluded that pepper could still keep its normal growth and development when the root zone temperature over 14 ℃.
引文
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[9]李荣富,梁莉,胡晓红,等.低温对杏花丙二醛含量及过氧化物酶与超氧化物歧化酶活性的影响[J].内蒙古农业科技,2005(6):29-30.
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[11]衣莹,张玉龙,郭志富,等.冬小麦叶片对低温胁迫的生理响应[J].华北农学报,2013,28(1):144-148.
[12]钟杰阳,张玉莲.低温对不同杏品种枝条中MDA含量和电导率的影响[J].天津农业科学,2013,19(5):93-96.
[13]徐传保,戴庆敏.低温胁迫对竹子3种渗透调节物质的影响[J].河南农业科学,2011,40(1):127-130.
[14]马艳青,戴雄泽.低温胁迫对辣椒抗寒性相关生理指标的影响[J].湖南农业大学学报:自然科学版,2000,26(6):461-462.
[15]Cristina R G Sales,Rafael V Ribeiro,Joaquim A G,et al.Superoxide dismutase and ascorbate peroxidase improve the recovery of photosynthesis in sugarcane plants subjected to water deficit and low substrate temperature[J].Plant Physiology and Biochemistry,2013,73:326-336.
[16]Lee S H,Singh A P,Chung G C.Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decreases in water transport.[J].Journal of Experimental Botany,2004,55(43):1733-1741.
[17]Lee S H,Ahn S J,Im Y J,et al.Differential impact of low temperature on fatty acid unsaturation and lipoxygenase activity in figleaf gourd and cucumber roots[J].Biochemical and Biophysical Research Communications,2005,330(4):1194-1198.
[18]郭洪雪,宋希云,燕增文,等.高温胁迫对小麦幼苗几个生理生化指标的影响[J].华北农学报,2007,22(Z1):71-74.
[19]李玉全,张海艳,沈法富.作物耐盐性的分子生物学研究进展[J].山东科学,2002,15(2):8-14.
[20]姜伟,崔世茂,张怡婷,等.KNO3、K2SO4及其混盐胁迫对辣椒幼苗生理生化特性的影响[J].华北农学报,2011,26(5):164-169.
[2]任志雨,王秀峰.根区温度对黄瓜幼苗保护性酶活性和膜脂过氧化作用的影响[J].中国蔬菜,2006(2):20-21.
[3]中科院上海植物生理所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999.
[4]任志雨,王秀峰.根区温度对作物生长和生理代谢的影响综述[J].天津农学院学报,2003,10(2):32-36.
[5]李美如,刘鸿先,王以柔.植物细胞中的抗寒物质及其与植物抗冷性的关系[J].植物生理学通讯,1995,31(5):328-334.
[6]邹志荣,陆帼一.低温对辣椒幼苗膜脂过氧化和保护酶系统变化的影响[J].西北农业学报,1994,3(3):51-56.
[7]柴文臣,马蓉丽,焦彦生,等.低温胁迫对不同辣椒品种生长及生理指标的影响[J].华北农学报,2010,25(2):168-171.
[8]李荣富,梁莉,胡晓红,等.杏叶片组织结构特征、质膜透性与花期耐寒力的关系[J].内蒙古农业科技,2005(5):15-16.
[9]李荣富,梁莉,胡晓红,等.低温对杏花丙二醛含量及过氧化物酶与超氧化物歧化酶活性的影响[J].内蒙古农业科技,2005(6):29-30.
[10]王永健,姜亦巍,曹宛虹,等.低温对不同品种黄瓜种子萌发、过氧化物酶及同工酶的影响[J].华北农学报,1995,10(2):72-76.
[11]衣莹,张玉龙,郭志富,等.冬小麦叶片对低温胁迫的生理响应[J].华北农学报,2013,28(1):144-148.
[12]钟杰阳,张玉莲.低温对不同杏品种枝条中MDA含量和电导率的影响[J].天津农业科学,2013,19(5):93-96.
[13]徐传保,戴庆敏.低温胁迫对竹子3种渗透调节物质的影响[J].河南农业科学,2011,40(1):127-130.
[14]马艳青,戴雄泽.低温胁迫对辣椒抗寒性相关生理指标的影响[J].湖南农业大学学报:自然科学版,2000,26(6):461-462.
[15]Cristina R G Sales,Rafael V Ribeiro,Joaquim A G,et al.Superoxide dismutase and ascorbate peroxidase improve the recovery of photosynthesis in sugarcane plants subjected to water deficit and low substrate temperature[J].Plant Physiology and Biochemistry,2013,73:326-336.
[16]Lee S H,Singh A P,Chung G C.Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decreases in water transport.[J].Journal of Experimental Botany,2004,55(43):1733-1741.
[17]Lee S H,Ahn S J,Im Y J,et al.Differential impact of low temperature on fatty acid unsaturation and lipoxygenase activity in figleaf gourd and cucumber roots[J].Biochemical and Biophysical Research Communications,2005,330(4):1194-1198.
[18]郭洪雪,宋希云,燕增文,等.高温胁迫对小麦幼苗几个生理生化指标的影响[J].华北农学报,2007,22(Z1):71-74.
[19]李玉全,张海艳,沈法富.作物耐盐性的分子生物学研究进展[J].山东科学,2002,15(2):8-14.
[20]姜伟,崔世茂,张怡婷,等.KNO3、K2SO4及其混盐胁迫对辣椒幼苗生理生化特性的影响[J].华北农学报,2011,26(5):164-169.