中药肾毒宁颗粒对慢性肾衰大鼠的肾保护作用及其机制研究
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摘要
目的:观察肾毒宁颗粒对2/3肾切除大鼠的肾保护作用及其机制。方法:将SD大鼠120只随机分为手术组(100只)和假手术组(20只),手术组大鼠行2/3肾切除术。术后2周将手术组随机分为西药组、模型对照组及肾毒宁颗粒低、中、高剂量组,每组各18只。治疗2个月后记录比较各组血肌酐(SCr)、尿素氮(BUN)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果:与假手术组相比,模型对照组SCr、BUN、MDA水平显著升高(P<0.01),SOD水平显著下降(P<0.01)。与模型对照组相比,各治疗组SCr、BUN、MDA水平均显著降低(P<0.01),SOD水平均显著升高(P<0.01),且呈剂量依赖关系。高剂量组与西药组SOD、MDA水平比较无统计学差异(P>0.05)。结论:中药肾毒宁颗粒能够有效改善CRF大鼠肾功能,其机理可能与其通过抗氧化作用,调节SOD、MDA水平有关。
Objective:To observe protective effect of Shendunning granule on 2/3 nephrectomized rats and its mechanism.Methods:Divided 120 SD rat into operation group(100 cases)and sham group(20 cases)randomly,and provided 2/3 nephrectomy to operation group.After 2 weeks of operation,divided operation groups into western medicine group,model control group,shenduning high,medium and low dose group randomLy with 18 cases in each group.Recorded and compared each group's serum creatinine(SCr),urea nitrogen(BUN),superoxide dismutase(SOD)and malondialdehyde(MDA)levels after 2 months' treatment.Results:Comparing with those in sham group,model control group's SCr,BUN and MDA levels increased significantly(P<0.01),but SOD decreased significantly(P<0.01).Comparing with those in model control group,each treatment group's SCr,BUN and MDA decreased significantly(P<0.01),but SOD increased significantly(P<0.01),and it is in a dose-dependent manner.Here were no significant differences if comparing SOD,MDA between shenduning high dose group and western medicine group(P>0.05).Conclusion:TCM shenduning granule helps rats with CRF to improve renal function,the mechanism maybe relates to its antioxidant to regulate SOD,MDA levels.
Objective:To observe protective effect of Shendunning granule on 2/3 nephrectomized rats and its mechanism.Methods:Divided 120 SD rat into operation group(100 cases)and sham group(20 cases)randomly,and provided 2/3 nephrectomy to operation group.After 2 weeks of operation,divided operation groups into western medicine group,model control group,shenduning high,medium and low dose group randomLy with 18 cases in each group.Recorded and compared each group's serum creatinine(SCr),urea nitrogen(BUN),superoxide dismutase(SOD)and malondialdehyde(MDA)levels after 2 months' treatment.Results:Comparing with those in sham group,model control group's SCr,BUN and MDA levels increased significantly(P<0.01),but SOD decreased significantly(P<0.01).Comparing with those in model control group,each treatment group's SCr,BUN and MDA decreased significantly(P<0.01),but SOD increased significantly(P<0.01),and it is in a dose-dependent manner.Here were no significant differences if comparing SOD,MDA between shenduning high dose group and western medicine group(P>0.05).Conclusion:TCM shenduning granule helps rats with CRF to improve renal function,the mechanism maybe relates to its antioxidant to regulate SOD,MDA levels.
引文
[1]熊荣兵,张婷,何立群,等.中药肾毒宁颗粒对慢性肾衰大鼠肾保护作用及其机制的实验研究[J].中国中医急症,2017,11(5):775-778.
[2]徐军建,苏衍进,曹君一.肾复宁颗粒抑制5/6肾切除CRF大鼠肾组织α-SMA表达的实验研究[J].陕西中医,2014,10(3):380-381.
[3]蓝文,蒋云生.中药肾复舒颗粒合用阿托品对肾衰大鼠残余肾VEGF和MVD的影响[J].中南药学,2014,12(5):175-177.
[4]雷根平,曾小晴,郭亚平.薯蓣丸对5/6肾切除慢性肾衰大鼠血清CrBUN及MMP-9TIMP-1影响的实验研究[J].四川中医,2014,15(11):35-37.
[5]王蓝天,王天然.槲皮素对慢性肾衰大鼠肾脏细胞的保护作用研究[J].西南国防医药,2014,24(2):144-146.
[6]胡蓉,王明清,刘阳阳,等.肾衰营养胶囊对5/6肾切除慢性肾衰大鼠微炎症状态血管损伤的抗炎作用[J].广东医学,2016,37(22):3345-3348.
[7]周颖,曾慧莲.尿毒清颗粒对慢性肾衰竭患者血脂及血清同型半胱氨酸的影响[J].中国基层医药,2015,20(14):2122-2124.
[8]永慧,王艳侠,于燕莉,等.消氮颗粒对腺嘌呤所致慢性肾衰模型大鼠的保护作用[J].中国药房,2014,15(39):3653-3655.
[9]尹翠翠,杨勇,容蓉,等.大黄配伍对慢性肾衰竭大鼠心钠素、内皮素的影响[J].山东中医杂志,2014,26(2):134-136.
[10]赵先锋.中医药治疗慢性肾衰竭研究进展[J].现代中西医结合杂志,2014,23(2):217-220.
[11]杨勇,王霄一,丁敏,等.肾衰宁胶囊对慢性肾功能衰竭患者营养状况及血液流变学的影响[J].中国基层医药,2017,24(10):177-179.
[12]赵静,刘宝利,张胜容,等.中成药治疗慢性肾衰竭现状分析[J].北京中医药,2014,33(5):368-370.
[2]徐军建,苏衍进,曹君一.肾复宁颗粒抑制5/6肾切除CRF大鼠肾组织α-SMA表达的实验研究[J].陕西中医,2014,10(3):380-381.
[3]蓝文,蒋云生.中药肾复舒颗粒合用阿托品对肾衰大鼠残余肾VEGF和MVD的影响[J].中南药学,2014,12(5):175-177.
[4]雷根平,曾小晴,郭亚平.薯蓣丸对5/6肾切除慢性肾衰大鼠血清CrBUN及MMP-9TIMP-1影响的实验研究[J].四川中医,2014,15(11):35-37.
[5]王蓝天,王天然.槲皮素对慢性肾衰大鼠肾脏细胞的保护作用研究[J].西南国防医药,2014,24(2):144-146.
[6]胡蓉,王明清,刘阳阳,等.肾衰营养胶囊对5/6肾切除慢性肾衰大鼠微炎症状态血管损伤的抗炎作用[J].广东医学,2016,37(22):3345-3348.
[7]周颖,曾慧莲.尿毒清颗粒对慢性肾衰竭患者血脂及血清同型半胱氨酸的影响[J].中国基层医药,2015,20(14):2122-2124.
[8]永慧,王艳侠,于燕莉,等.消氮颗粒对腺嘌呤所致慢性肾衰模型大鼠的保护作用[J].中国药房,2014,15(39):3653-3655.
[9]尹翠翠,杨勇,容蓉,等.大黄配伍对慢性肾衰竭大鼠心钠素、内皮素的影响[J].山东中医杂志,2014,26(2):134-136.
[10]赵先锋.中医药治疗慢性肾衰竭研究进展[J].现代中西医结合杂志,2014,23(2):217-220.
[11]杨勇,王霄一,丁敏,等.肾衰宁胶囊对慢性肾功能衰竭患者营养状况及血液流变学的影响[J].中国基层医药,2017,24(10):177-179.
[12]赵静,刘宝利,张胜容,等.中成药治疗慢性肾衰竭现状分析[J].北京中医药,2014,33(5):368-370.