七叶一枝花提取物对小鼠的止血作用
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摘要
采用低、中、高剂量(5、10、15 g/kg)的七叶一枝花水提物和醇提物对小鼠进行灌胃处理,分别作用0.5、1、2和4 h后,测定小鼠的止血时间(BT)及出血量,研究七叶一枝花(陕产重楼)提取物对小鼠的止血作用。同时,采用眼眶取血测定血小板数目、血小板压积、血小板体积分布宽度、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)及纤维蛋白原(FIB)等血小板和血液凝集指标。结果显示:低、中、高剂量组的水提物与醇提物均可显著性降低小鼠BT和出血量,并在中、高剂量组呈极显著性降低;高剂量组在给药0.5、1和4 h时BT显著性降低,在给药2 h时极显著性降低,但出血量只在2 h时呈显著性降低;并且醇提比水提显示出更显著的止血作用。在血小板及血液凝集指标中,血小板数目、压积、体积分布宽度及FIB均极显著性升高,但PT、APTT无显著性变化。结果说明七叶一枝花提取物对小鼠具有很好的止血效果,在醇提高剂量组作用2 h时止血效果最好;其可能主要是通过增加血小板基数、激活血小板发生形变、提高FIB数值,来促进小鼠止血。
In order to study the hemostatic effect of extractive from Paris polyphylla for mice, low, medium, and high doses(5, 10, and 15 g/kg) of Paris polyphylla water extractive and alcohol extractive were used. The mice were treated with gavage for 0.5, 1, 2 and 4 h, and the hemostatic time(BT) and bleeding were measured and compared its′ effects. At the same time, platelet count, platelet pressure, platelet volume distribution width, prothrombin time(PT), activated partial thromboplastin time(APTT), and fibrinogen(FIB) platelet and blood coagulation indexes were measured using eyelid sampling. The results showed that the water extractive and alcohol extractive of low dose group decreased significantly on BT and blood loss in mice, and middle and high dose groups very decreased significantly.The high-dose group significantly decreased in 0.5, 1 and 4 h, and very decreased significantly in 2 h, but the amount of bleeding was significantly lower in 2 h.The hemostatic effect of alcohol extractive is better than that of water extractive. In platelet and blood agglutination indexes, platelet number, volume, volume distribution width, and FIB were all significantly higher, but there was no significant change in PT and APTT. The results showed that Paris polyphylla extractive has a good hemostatic effect on mice, and the effect of hemostasis is best incase of alcohol extractive with higher dosage for 2 hours. The main effect is to increase the platelet number, activate platelet degeneration, and increase the FIB value for promotes hemostasis in mice.
In order to study the hemostatic effect of extractive from Paris polyphylla for mice, low, medium, and high doses(5, 10, and 15 g/kg) of Paris polyphylla water extractive and alcohol extractive were used. The mice were treated with gavage for 0.5, 1, 2 and 4 h, and the hemostatic time(BT) and bleeding were measured and compared its′ effects. At the same time, platelet count, platelet pressure, platelet volume distribution width, prothrombin time(PT), activated partial thromboplastin time(APTT), and fibrinogen(FIB) platelet and blood coagulation indexes were measured using eyelid sampling. The results showed that the water extractive and alcohol extractive of low dose group decreased significantly on BT and blood loss in mice, and middle and high dose groups very decreased significantly.The high-dose group significantly decreased in 0.5, 1 and 4 h, and very decreased significantly in 2 h, but the amount of bleeding was significantly lower in 2 h.The hemostatic effect of alcohol extractive is better than that of water extractive. In platelet and blood agglutination indexes, platelet number, volume, volume distribution width, and FIB were all significantly higher, but there was no significant change in PT and APTT. The results showed that Paris polyphylla extractive has a good hemostatic effect on mice, and the effect of hemostasis is best incase of alcohol extractive with higher dosage for 2 hours. The main effect is to increase the platelet number, activate platelet degeneration, and increase the FIB value for promotes hemostasis in mice.
引文
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[3] ZHAO Y,KANG L P,LIU Y X,et al.Three new steroidal saponins form the rhizome of paris polyphylla[J].Magnetic Resonance in Chemistry,2007,45:739-744.
[4] 赵保胜,马勇,贵海水,等.中药重楼研究进展[J].中国实验方剂学杂志,2011,17(11):267-269.
[5] MAN S L,GAO W Y,ZHANG Y J,et al.Antitumor and antimetastatic activities of Rhizoma paridis saponins[J].Steroids,2009,74:1051-1056.
[6] ZHANG X F,CUI Y,HUANG J J,et al..Immuno-stimulating properties of diosgenyl saponins isolated from paris polyphylla[J].Bioorganis&Medicinal Chemistry Letters,2007,17:2408-2413.
[7] PAN Y M,LIANG Y,WANG H S,et al..Antioxidant activities of several Chinese medicine herbs[J].Food Chemistry,2004,88:347-350.
[8] 何汉杰,章怀云,陈丽莉,等.重楼皂苷的药理作用和临床应用研究进展[J].中药材,2014,37(3):527-530.
[9] 王玲,哈孝贤,屈彩芹,等.功血宁1号冲剂对止血作用的影响[J].天津中医,2002,19(5):41-43.
[10] 吴夏慧.三种天然总皂苷的提取分离和抗肿瘤作用研究[D].南京:南京大学,2011.
[11] 孙治国,张琳,李凌军,等.重楼总皂苷提取工艺研究[J].中药材,2007,30(6):726-729.
[12] 吴捷,赵进,孟照俊,等.明目止血片对动物止血作用的实验观察[J].山东中医杂志,2003,22(7):426-428.
[13] 尹鸿翔,文飞燕,张浩.彝药“麻补”止血活性物质基础及机理研究[J].世界科学技术—中医药现代化,2014,16(1):177-180.
[14] 罗刚,吴延楷,周永禄,等.重楼皂苷止血作用的初步研究[J].中药药理与临床,1998,4(2):37-39.
[15] 孙磊.凝血酶原时间和活化部分凝血酶原时间测定的问题[J].中国医药指南,2013,11(5):177-178.
[16] 黄彩芝,莫丽亚,邓永超,等.重症肺炎患儿血小板参数与凝血指标的探讨[J].中国感染控制杂志,2015,14(2):98-100.
[17] 付亚莉,赵振虎,善亚军,等.重楼甾体总皂苷对血小板聚集的直接诱导作用及初步机制研究[J].军事医学科学院院刊,2007,31(5):416-419.