利用除草剂来高效加倍甜玉米单倍体
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摘要
本试验通过比较双草醚、氟乐灵、乙草胺3种除草剂种在溶液浓度为40μmol/L、60μmol/L、80μmol/L时,采用浸种的加倍方法,分别处理经GT5号诱导系诱导的‘吉甜99’、‘吉甜188’、‘甜玉6号’甜玉米单倍体,选出最适合甜玉米单倍体加倍的方法。试验结果表明,3种不同类型的除草剂对甜玉米单倍体都有明显的加倍效果,不同浓度的药剂加倍效率不同。其中,经双草醚处理的加倍率为3.42%～9.39%,氟乐灵加倍率3.87%～12.34%,乙草胺加倍率为1.85%～9.28%,加倍效果最好的是80μmol/L的氟乐灵处理单倍体‘吉甜99’,加倍率最高可达12.34%。3种浓度的除草剂处理甜玉米单倍体的加倍率均达到显著差异,说明除草剂乙草胺、氟乐灵、双草醚均能有效加倍甜玉米单倍体。从而为提高甜玉米单倍体加倍效率的研究提供理论帮助。
In this experiment, three kinds of herbicides, bispyribac-sodium, trifluralin and acetochlor, were used to make solution with concentration of 40 μmol/L, 60 μmol/L, and 80 μmol/L. Sweet corn haploid, 'Jitian 99', 'Jitian188', and 'Tianyu 6 'which were induced by GT5 inducing line, were soaked in the solution to select the optimal method for haploid doubling in sweet corn. The results showed that three different types of herbicides had obvious double effect on sweet corn haploid, and different concentrations had different efficiencies. Among them, the double rates of bispyribac-sodium, trifluralin and acetochlor were 3.42%～9.39%, 3.87%～12.34%, and 1.85%～9.28%, respectively. The best doubled effect was haploid 'Jitian 99' treated by 80 μmol/L trifluralin, and the double rate was up to 12.34%. The doubling rates of three kinds of herbicide on sweet corn haploid all reached significant differences, indicating that bispyribac-sodium, acetochlor, and trifluralin could effectively double the sweet corn haploid. This research could provide theoretical help for the research on the efficiency improvement of doubling the haploid of sweet corn.
In this experiment, three kinds of herbicides, bispyribac-sodium, trifluralin and acetochlor, were used to make solution with concentration of 40 μmol/L, 60 μmol/L, and 80 μmol/L. Sweet corn haploid, 'Jitian 99', 'Jitian188', and 'Tianyu 6 'which were induced by GT5 inducing line, were soaked in the solution to select the optimal method for haploid doubling in sweet corn. The results showed that three different types of herbicides had obvious double effect on sweet corn haploid, and different concentrations had different efficiencies. Among them, the double rates of bispyribac-sodium, trifluralin and acetochlor were 3.42%～9.39%, 3.87%～12.34%, and 1.85%～9.28%, respectively. The best doubled effect was haploid 'Jitian 99' treated by 80 μmol/L trifluralin, and the double rate was up to 12.34%. The doubling rates of three kinds of herbicide on sweet corn haploid all reached significant differences, indicating that bispyribac-sodium, acetochlor, and trifluralin could effectively double the sweet corn haploid. This research could provide theoretical help for the research on the efficiency improvement of doubling the haploid of sweet corn.
引文
Cai Z.,Xu G.L.,Lu M.,and Zhang H.Y.,2008,Advances in research on maize haploid breeding,Yumi Kexue(Journal of Maize Sciences),16(1):1-5(才卓,徐国良,路明,张洪艳,2008,玉米单倍体育种研究进展,玉米科学,16(1):1-5)
Chen S.P.,Wang X.Z.,Tang H.J.,and Xi X.Z.,2011,The distant hybridization technology of wheat×maize system and application evaluation of haploid breeding,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),9(4):519-524(陈淑萍,王雪征,唐怀君,茜晓哲,2011,小麦×玉米远缘杂交技术以及在单倍体育种上的应用评价,分子植物育种,9(4):519-524
Deng K.P.,Li X.Y.,Jing G.X.,Lu B.,Xing Z.,and Zhao R.G.,2017,Preliminary study on efficient Waxy maize haploid doubling metho,Zhongzi(Seeds),36(1):82-85(邓昆鹏,李向永,景桂昕,鲁彬,邢政,赵仁贵,2017,高效糯玉米单倍体加倍方法的研究,种子,36(1):82-85)
Jing G.X.,Wang X.Q.,Li X.Y.,Xing Z.,Deng K.P.,Ren X.C.,and Zhao R.N.,2017,Doubling efficiency of Waxy maize haploids treated by different new herbicides,Zhongzi(Seeds),36(5):71-73(景桂昕,王薪淇,李向永,邢政,邓昆鹏,任孝慈,赵仁贵,2017,不同新型除草剂加倍糯玉米单倍体的效率研究,种子,36(5):71-73)
Wang H.,Li J.Z.,Zhang Z.W.,Ci J.B.,Jiang L.,Zhang Y.,and Yang W.G.,2013,Study on doubling efficiency of maize haploids bud soaked by herbicide and colchicine,Jilin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),35(4):384-388(王贺,李继竹,张继伟,慈佳宾,姜龙,张野,杨伟光,2013,秋水仙素和除草剂浸芽加倍玉米单倍体效率的研究,吉林农业大学学报,35(4):384-388)
Wang X.D.,2014,Research status and development trend for sweet corn breeding in China,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(10):146-148(王晓东,2014,我国甜玉米育种的研究现状及发展趋势,黑龙江农业科学,(10):146-148)
Wei C.S.,Xu G.M.,and Tian F.H.,2014,Research progresses of the identification methods on maize haploid,Zuowu Xuebao(Crops),(6):17-20(魏昌松,许贵明,田甫焕,2014,玉米单倍体鉴定方法研究进展,作物杂志,(6):17-20)
Wei J.J.,Zhang X.L.,Chen M.X.,Liu Z.Z.,and Zhu L.Y.,2007,Analysis of the results of Injection treatment with colchicines in six leaf stage to maize haploid,Yumi Kexue(Journal of Maize Sciences),15(4):49-51(魏俊杰,张晓丽,陈梅香,刘志增,祝丽英.,2007,6叶期秋水仙素注射处理玉米单倍体的加倍效果研究,玉米科学,15(4):49-51)
Wu M.Y.,2010,Selective breeding of sweet corn varieties and its current industrial development,Nongye Keji Yu Zhuangbei(Agricultural Science&Technology and Equipment),(5):54-55(武明宇,2010,甜玉米品种选育及产业发展现状,农业科技与装备,(5):54-55)
Zheng J.R.,Han F.G.,and Li Z.J.,2009,The status quo and development trend of sweet corn industry at home and abroad,Guangdong Nongye Kexue(Guangdong Agricultural Sciences),(10):35-38(郑锦荣,韩福光,李智军,2009,国内外甜玉米产业现状与发展趋势,广东农业科学,(10):35-38)
Chen S.P.,Wang X.Z.,Tang H.J.,and Xi X.Z.,2011,The distant hybridization technology of wheat×maize system and application evaluation of haploid breeding,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),9(4):519-524(陈淑萍,王雪征,唐怀君,茜晓哲,2011,小麦×玉米远缘杂交技术以及在单倍体育种上的应用评价,分子植物育种,9(4):519-524
Deng K.P.,Li X.Y.,Jing G.X.,Lu B.,Xing Z.,and Zhao R.G.,2017,Preliminary study on efficient Waxy maize haploid doubling metho,Zhongzi(Seeds),36(1):82-85(邓昆鹏,李向永,景桂昕,鲁彬,邢政,赵仁贵,2017,高效糯玉米单倍体加倍方法的研究,种子,36(1):82-85)
Jing G.X.,Wang X.Q.,Li X.Y.,Xing Z.,Deng K.P.,Ren X.C.,and Zhao R.N.,2017,Doubling efficiency of Waxy maize haploids treated by different new herbicides,Zhongzi(Seeds),36(5):71-73(景桂昕,王薪淇,李向永,邢政,邓昆鹏,任孝慈,赵仁贵,2017,不同新型除草剂加倍糯玉米单倍体的效率研究,种子,36(5):71-73)
Wang H.,Li J.Z.,Zhang Z.W.,Ci J.B.,Jiang L.,Zhang Y.,and Yang W.G.,2013,Study on doubling efficiency of maize haploids bud soaked by herbicide and colchicine,Jilin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),35(4):384-388(王贺,李继竹,张继伟,慈佳宾,姜龙,张野,杨伟光,2013,秋水仙素和除草剂浸芽加倍玉米单倍体效率的研究,吉林农业大学学报,35(4):384-388)
Wang X.D.,2014,Research status and development trend for sweet corn breeding in China,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(10):146-148(王晓东,2014,我国甜玉米育种的研究现状及发展趋势,黑龙江农业科学,(10):146-148)
Wei C.S.,Xu G.M.,and Tian F.H.,2014,Research progresses of the identification methods on maize haploid,Zuowu Xuebao(Crops),(6):17-20(魏昌松,许贵明,田甫焕,2014,玉米单倍体鉴定方法研究进展,作物杂志,(6):17-20)
Wei J.J.,Zhang X.L.,Chen M.X.,Liu Z.Z.,and Zhu L.Y.,2007,Analysis of the results of Injection treatment with colchicines in six leaf stage to maize haploid,Yumi Kexue(Journal of Maize Sciences),15(4):49-51(魏俊杰,张晓丽,陈梅香,刘志增,祝丽英.,2007,6叶期秋水仙素注射处理玉米单倍体的加倍效果研究,玉米科学,15(4):49-51)
Wu M.Y.,2010,Selective breeding of sweet corn varieties and its current industrial development,Nongye Keji Yu Zhuangbei(Agricultural Science&Technology and Equipment),(5):54-55(武明宇,2010,甜玉米品种选育及产业发展现状,农业科技与装备,(5):54-55)
Zheng J.R.,Han F.G.,and Li Z.J.,2009,The status quo and development trend of sweet corn industry at home and abroad,Guangdong Nongye Kexue(Guangdong Agricultural Sciences),(10):35-38(郑锦荣,韩福光,李智军,2009,国内外甜玉米产业现状与发展趋势,广东农业科学,(10):35-38)