甜玉米孤雌生殖诱导单倍体
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摘要
本研究以诱导系高诱5和Stock6为父本分别对杂交种BT1～BT10作杂交诱导,对长花丝和短花丝两种处理进行方差分析。其中对诱导率的分析表明,高诱5的诱导率普遍高于Stock6,但未达到显著水平。其中高诱5诱导的甜玉米杂交种中,BT3诱导率最高,达8.15%;Stock6诱导的甜玉米杂交种中,同样是BT3的诱导率最高,达7.69%。YD1长花丝处理诱导率达9.19%,短花丝处理达6.09%。YD2长花丝处理诱导率达7.32%,短花丝处理达5.37%。结果表明,不同母本材料对单倍体的诱导率有影响,长、短花丝对单倍体的诱导率呈显著性差异。因此,选择适宜的甜玉米单倍体诱导方式,也能够提高甜玉米的诱导率,从而进一步提高甜玉米DH育种效率。
In this research, hybrids BT1～BT10 were induced by using crossing lines Gaoyou5 and Stock6 as male parent, respectively. The variance analysis was performed on the long-filament and short-filament silk fibroin.Among them, the analysis of the induction rate showed that the induction rate of Gaoyou5 was generally higher than that of Stock6, but it did not reach the significant level. In sweet corn hybrids induced by Gaoyou5, BT3 had the highest induction rate which was 8.15%. Moreover, in the stock6-induced sweet corn hybrids, BT3 also had the highest induction rate, which was 7.69%. The induction rates of YD1 long filament treatment and short filament treatment were 9.19% and 6.09%, respectively. While the induction rates of YD2 long filament treatment and short filament treatment reached 7.32% and 5.37%, respectively. The results indicated that different maternal materials had an impact on the haploid induction rate, and the induction rate of haploid was significantly different between long and short fillers. Therefore, the selection of suitable sweet maize haploid induction mode could also improve the sweet corn induction rate, thereby further improving the efficiency of sweet corn DH breeding.
In this research, hybrids BT1～BT10 were induced by using crossing lines Gaoyou5 and Stock6 as male parent, respectively. The variance analysis was performed on the long-filament and short-filament silk fibroin.Among them, the analysis of the induction rate showed that the induction rate of Gaoyou5 was generally higher than that of Stock6, but it did not reach the significant level. In sweet corn hybrids induced by Gaoyou5, BT3 had the highest induction rate which was 8.15%. Moreover, in the stock6-induced sweet corn hybrids, BT3 also had the highest induction rate, which was 7.69%. The induction rates of YD1 long filament treatment and short filament treatment were 9.19% and 6.09%, respectively. While the induction rates of YD2 long filament treatment and short filament treatment reached 7.32% and 5.37%, respectively. The results indicated that different maternal materials had an impact on the haploid induction rate, and the induction rate of haploid was significantly different between long and short fillers. Therefore, the selection of suitable sweet maize haploid induction mode could also improve the sweet corn induction rate, thereby further improving the efficiency of sweet corn DH breeding.
引文
Ci J.B.,Yang W.,Ren X.J.,Cui X.Y.,Zhang Y.,Zhang Y.H.,and Yang W.G.,2015,Studies on maize haploid inducing and chemical doubling monoploids,Huanan Nongye Daxue Xuebao(Journal of South China Agricultural University),36(3):49-53(慈佳宾,杨巍,任雪娇,崔学宇,张野,2015,玉米单倍体诱导及化学加倍方法的研究,华南农业大学学报,36(3):49-53)
Hu G.H.,2009,Investigation of male haploid induction techniques for inbred,Line development in maize,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(1):138-139(扈光辉,2009,利用父本单倍体诱导系选育自交系的研究进展,黑龙江农业科学,(1):138-139)
Jiang L.,Ci J.B.,Cui X.Y.,Zhang Y.,Nan N.,Li J.Z.,and Yang W.G.,2014,Study on induction rate and doubling rate of maize haploid under,different ecological conditions,Jilin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),36(2):139-143(姜龙,慈佳宾,崔学宇,张野,南楠,李继竹,杨伟光,2014,不同生态条件下玉米单倍体诱导率和加倍率研究,吉林农业大学学报,36(2):139-143)
Jiang L.,Nan N.,Wang X.Q.,Li X.Y.,Jing G.X.,Xing Z.,and Zhao R.G.,2016,Haploid inducing and doubling methods for sweet corn,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University),44(7):95-100(姜龙,南楠,王薪淇,李向永,景桂昕,邢政,赵仁贵,2016,甜玉米单倍体诱导和加倍方法研究,西北农林科技大学学报,44(7):95-100)
Li G.K.,Hu J.G.,Zheng J.R.,Liu J.H.,and Han F.G.,2013,Research on haploid efficiently inducing of sweet corn,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),34(12):2397-2402(李高科,胡建广,郑锦荣,刘建华,韩福光,2013,甜玉米单倍体高效诱导研究,热带作物学报,34(12):2397-2402)
Lv G.H.,Xu X.H.,Lu H.B.,and Guo G.J.,2012,Primary research on corn haploid breeding,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),24(6):957-960(吕桂华,徐秀红,卢华兵,郭国锦,2012,单倍体诱导系在玉米育种研究中的初探,浙江农业学报,24(6):957-960)
Wang J.H.,You S.,Zhao A.J.,Gao Z.Y.,Chen X.Y.,and Guan Z.B.,2016,Study on maize haploid inducing and doubling method and application,Hebei Nongye Kexue(Journal of Hebei Agricultural Sciences),20(1):70-75(王江浩,尤帅,赵爱菊,高增玉,陈希勇,关中波,2016,玉米单倍体的诱导加倍技术及其应用研究,河北农业科学,20(1):70-75)
Wang Z.,and Xu W.J.,2004,Research development of seed vigor of sweet maize,Zhongzi(Seed),23(7):46-49(王仲,许文娟,2004,甜玉米种子活力研究进展,种子,23(7):46-49)
Yao W.H.,Han X.L.,Wang Y.F.,Tan J.,Xu C.X.,Chen H.M.,and Fan X.M.,2011,Research status and development strategy for sweet corn breeding in China,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),13(2):1-8(姚文华,韩学莉,汪燕芬,谭静,徐春霞,陈洪梅,番兴明,2011,我国甜玉米育种研究现状与发展对策,中国农业科技导报,13(2):1-8)
Yue Y.,Zheng Y.Z.,Wang T.J.,Ni Y.C.,Dong B.C.,Gao W.,Kang H.,and Sun S.W.,2013,Correlation study on growth and development rules and filament vigor of corn,Anhui Nongxue Tongbao(Auhui Agricultural Science Bulletin),19(18):16-17(岳杨,郑永照,王提江,倪玉春,董本春,高玮,康恒,孙世伟,2013,玉米花丝生长发育规律及花丝活力的相关研究,安徽农学通报,19(18):16-17)
Hu G.H.,2009,Investigation of male haploid induction techniques for inbred,Line development in maize,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(1):138-139(扈光辉,2009,利用父本单倍体诱导系选育自交系的研究进展,黑龙江农业科学,(1):138-139)
Jiang L.,Ci J.B.,Cui X.Y.,Zhang Y.,Nan N.,Li J.Z.,and Yang W.G.,2014,Study on induction rate and doubling rate of maize haploid under,different ecological conditions,Jilin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),36(2):139-143(姜龙,慈佳宾,崔学宇,张野,南楠,李继竹,杨伟光,2014,不同生态条件下玉米单倍体诱导率和加倍率研究,吉林农业大学学报,36(2):139-143)
Jiang L.,Nan N.,Wang X.Q.,Li X.Y.,Jing G.X.,Xing Z.,and Zhao R.G.,2016,Haploid inducing and doubling methods for sweet corn,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University),44(7):95-100(姜龙,南楠,王薪淇,李向永,景桂昕,邢政,赵仁贵,2016,甜玉米单倍体诱导和加倍方法研究,西北农林科技大学学报,44(7):95-100)
Li G.K.,Hu J.G.,Zheng J.R.,Liu J.H.,and Han F.G.,2013,Research on haploid efficiently inducing of sweet corn,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),34(12):2397-2402(李高科,胡建广,郑锦荣,刘建华,韩福光,2013,甜玉米单倍体高效诱导研究,热带作物学报,34(12):2397-2402)
Lv G.H.,Xu X.H.,Lu H.B.,and Guo G.J.,2012,Primary research on corn haploid breeding,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),24(6):957-960(吕桂华,徐秀红,卢华兵,郭国锦,2012,单倍体诱导系在玉米育种研究中的初探,浙江农业学报,24(6):957-960)
Wang J.H.,You S.,Zhao A.J.,Gao Z.Y.,Chen X.Y.,and Guan Z.B.,2016,Study on maize haploid inducing and doubling method and application,Hebei Nongye Kexue(Journal of Hebei Agricultural Sciences),20(1):70-75(王江浩,尤帅,赵爱菊,高增玉,陈希勇,关中波,2016,玉米单倍体的诱导加倍技术及其应用研究,河北农业科学,20(1):70-75)
Wang Z.,and Xu W.J.,2004,Research development of seed vigor of sweet maize,Zhongzi(Seed),23(7):46-49(王仲,许文娟,2004,甜玉米种子活力研究进展,种子,23(7):46-49)
Yao W.H.,Han X.L.,Wang Y.F.,Tan J.,Xu C.X.,Chen H.M.,and Fan X.M.,2011,Research status and development strategy for sweet corn breeding in China,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),13(2):1-8(姚文华,韩学莉,汪燕芬,谭静,徐春霞,陈洪梅,番兴明,2011,我国甜玉米育种研究现状与发展对策,中国农业科技导报,13(2):1-8)
Yue Y.,Zheng Y.Z.,Wang T.J.,Ni Y.C.,Dong B.C.,Gao W.,Kang H.,and Sun S.W.,2013,Correlation study on growth and development rules and filament vigor of corn,Anhui Nongxue Tongbao(Auhui Agricultural Science Bulletin),19(18):16-17(岳杨,郑永照,王提江,倪玉春,董本春,高玮,康恒,孙世伟,2013,玉米花丝生长发育规律及花丝活力的相关研究,安徽农学通报,19(18):16-17)