不同玉米单倍体诱导系之间杂交组合诱导性状的杂种优势研究
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摘要
以不同血缘的8份诱导系及按照NCⅡ组配的28份诱导系间杂交组合F1作为试验材料,分析株高、雄穗小花数的杂种优势,并以5份不同类群骨干自交系龙抗11、S 121、郑58、吉853、丹599作为测验种进行诱导率测验,分析不同单倍体诱导系间杂交组合F1诱导率的杂种优势。结果表明,对于株高性状,共计24份诱导系F1株高表现出超亲优势,最高超高亲值32.7cm;对于诱导率性状,共计15份F1诱导率表现出超亲优势,最高超高亲值2.36%;对于雄穗小花数性状,28份诱导系F1的雄穗小花数均表现出超亲优势,位于前5位的组合为DW 26/DW 27(602.7)、DW 8/DW 23(574.7)、DW 23/DW 27(554.7)、DW 24/DW 27(538.7)、DW 22/DW 27(522.7);综合诱导率、株高、雄穗小花数等3个性状分析,表现优良的诱导系间杂交组合有DW 24/DW 25、DW 25/DW 26、DW 23/DW 27和DW 23/DW 24。
Using eight inducers from different consanguinity and 28 inducer hybrid combinations F1 plants according to the NCⅡequipping as experimental materials,we analyzed heterosis of plant height and floret number of male tassel.Also,using Longkang11,S 121,Z 58,Ji 853 and Dan 599 of backbone maize inbred lines from 5 different taxa as test species for induction rate test,we analyzed heterosis of F1 plants induction rate from different cross combinations between haploid induction system.The results indicated that for plant height,a total of twenty-fourth inducers of F1 plants presented heterobeltiosis,and the highest value of superiority over parents was 32.7 cm.For the induction rate traits,a total of fifteen inducers of F1 plants showed heterobeltiosis,the highest value of superiority over parents was 2.36%.For the floret number character of male tassel,the floret number of male tassel in the 28 inducers of F1 plants all showed heterobeltiosis and the top five combinations were DW 26/DW 27(602.7),DW 8/DW 23(574.7),DW 23/DW 27(554.7),DW 24/DW 27(538.7)and DW 22/DW 27(522.7).Based on the comprehensive analysis of three traits including combined induction rate,plant height and number of florets in male tassel,the inducer hybridization combination with excellent performance were DW 24/DW 25,DW 25/DW 26,DW23/DW 27 and DW 23/DW 24.
Using eight inducers from different consanguinity and 28 inducer hybrid combinations F1 plants according to the NCⅡequipping as experimental materials,we analyzed heterosis of plant height and floret number of male tassel.Also,using Longkang11,S 121,Z 58,Ji 853 and Dan 599 of backbone maize inbred lines from 5 different taxa as test species for induction rate test,we analyzed heterosis of F1 plants induction rate from different cross combinations between haploid induction system.The results indicated that for plant height,a total of twenty-fourth inducers of F1 plants presented heterobeltiosis,and the highest value of superiority over parents was 32.7 cm.For the induction rate traits,a total of fifteen inducers of F1 plants showed heterobeltiosis,the highest value of superiority over parents was 2.36%.For the floret number character of male tassel,the floret number of male tassel in the 28 inducers of F1 plants all showed heterobeltiosis and the top five combinations were DW 26/DW 27(602.7),DW 8/DW 23(574.7),DW 23/DW 27(554.7),DW 24/DW 27(538.7)and DW 22/DW 27(522.7).Based on the comprehensive analysis of three traits including combined induction rate,plant height and number of florets in male tassel,the inducer hybridization combination with excellent performance were DW 24/DW 25,DW 25/DW 26,DW23/DW 27 and DW 23/DW 24.
引文
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[14]徐小炜,董昕,黎亮,等.玉米单倍体诱导系间杂交种评价及其杂种优势分析及其杂交组合选育[J].中国农业大学学报,2014,19(4):1-6.
[15]董昕.玉米单倍体诱导基因qhir1精细定位与新型诱导系选育研究[D].北京:中国农业大学,2014.
[2]林霞,罗勇,唐秉晖.等.品种间杂交诱导玉米孤雌生殖单倍体的研究[J].陕西农业科学,2017,63(2):17-18.
[3]徐小炜,董昕,黎亮,等.玉米单倍体诱导系间杂交种评价及其杂种优势分析及其杂交组合选育[J].中国农业大学学报,2014,19(4):1-6.
[4]Kebede A.Z.,Dhillon B.S.,Schipprack W.,et al.Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize[J].Euphytica,2011,180:219-226.
[5]唐启义,冯光明.实用统计分析及其DPS数据处理系统[M].北京:中国农业出版社,2002.
[6]陈绍江,黎亮,李浩川,等.玉米单倍体育种技术[M].第2版.北京:中国农业大学出版社,2012.
[7]张如养,段民孝,赵久然,等.玉米单倍体诱导系诱导性状的杂种优势分析[J].种子,2016,35(1):77-80.
[8]杨钊钊.基于多个群体的玉米花期及雄穗相关性状的QTL分析[D].北京:中国农业科学院,2012.
[9]孙贵星.玉米DH育种关键技术及DH系遗传性研究[D].长春:吉林农业大学,2016.
[10]黎亮,李浩川,徐小炜,等.玉米孤雌生殖单倍体诱导效率优化方法研究[J].中国农业大学学报,2012,17(1):9-13.
[11]张如养,段民孝,赵久然,等.单倍体技术在玉米种质改良和育种中的应用方向[J].作物杂志,2012(5):4-8.
[12]施标,郑洪建,孙大鹏,等.CIMMYT玉米DH育种研究进展:诱导策略及应用[J].上海农业学报,2013,29(1):91-94.
[13]徐玉波,任保财,张宝石,等.玉米单倍体诱导系的杂种优势利用研究[J].辽宁农业科学,2010(3):40-43.
[14]徐小炜,董昕,黎亮,等.玉米单倍体诱导系间杂交种评价及其杂种优势分析及其杂交组合选育[J].中国农业大学学报,2014,19(4):1-6.
[15]董昕.玉米单倍体诱导基因qhir1精细定位与新型诱导系选育研究[D].北京:中国农业大学,2014.