响应面法优化忍冬器官中四种成分的超声提取工艺
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摘要
采用响应面法优化盐碱地栽培忍冬(Lonicera japonica Thunb.)器官综合物中咖啡酸、绿原酸、木犀草苷、多糖4种成分同时超声提取的最佳条件。以咖啡酸、绿原酸、木犀草苷、多糖4种成分总含量为响应值,通过Box-Behnken响应面法对提取时间、温度、料液比、乙醇体积分数这4个主要因素进行考察。结果表明,最优提取条件为提取时间1 h,温度75℃,料液比1∶40(g∶m L),乙醇体积分数为45%。应用超声提取技术能够在盐碱地栽培的忍冬器官综合物中同时获得一定含量的绿原酸、多糖、咖啡酸、木犀草苷等产物,具有提取效率高、节约资源等优势。
An ultrasonic-assisted extraction method was established to simultaneously produce the chlorogenic acid,cafferic acid,luteoloside and polysaccharose from Lonicera japonica Thunb. in saline-alkali land. Using the total concentration chlorogenic acid,cafferic acid,luteoloside and polysaccharose of as response value,Box-Behnken response surface design was adopted to investigate the four main factors of extraction time,temperature,solid/liquid ratio and ethanol concentration. The results showed that the optimization conditions for extraction were extraction time 1 h,temperature 75 ℃,and solid/liquid ratio 1∶40(g∶m L) and ethanol concentration 45%. The ultrasonic-assisted extraction of the four components has the advantage of accelerating the extraction process and better products with lower cost.
An ultrasonic-assisted extraction method was established to simultaneously produce the chlorogenic acid,cafferic acid,luteoloside and polysaccharose from Lonicera japonica Thunb. in saline-alkali land. Using the total concentration chlorogenic acid,cafferic acid,luteoloside and polysaccharose of as response value,Box-Behnken response surface design was adopted to investigate the four main factors of extraction time,temperature,solid/liquid ratio and ethanol concentration. The results showed that the optimization conditions for extraction were extraction time 1 h,temperature 75 ℃,and solid/liquid ratio 1∶40(g∶m L) and ethanol concentration 45%. The ultrasonic-assisted extraction of the four components has the advantage of accelerating the extraction process and better products with lower cost.
引文
[1]李平,赵成.金银花水提物及醇提物体外抗菌实验[J].中国当代医药,2010,17(17):48-50.
[2]胡克杰,王跃红,王栋.金银花中绿原酸在体外抗病毒作用的实验研究[J].中医药信息,2010,7(3):27-28.
[3]刘雄,高建德.金银花质量控制、化学成分及药理学研究进展[J].甘肃中医学院学报,2006,23(4):46-48.
[4]国家药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015.
[5]韩飞飞,吴绵斌,孔繁智.天然活性多糖药理作用研究进展[J].西北药学杂志,2006,21(2):91-92.
[6]王宗成,彭延波,盘雪,等.响应面设计优化苎麻叶黄酮提取工艺[J].中国饲料,2016,19(3):12-15.
[7]罗毅,潘细贵,刘刚,等.苯酚-硫酸法测定多糖含量显色方式的优选[J].中国中医药信息杂志,2005,12(1):45-46.
[8]石奇.酶辅助提取金银花中绿原酸与多糖的工艺研究[J].应用化工,2015,44(7):1268-1271.
[9]雷艳萍,石晓峰,刘东彦,等.响应面法优化雪松松针中总木脂素的提取工艺[J].中国药房,2016,27(7):939-942.
[10]薛迎迎,杨汝德,齐瀚实,等.胶束溶液中微生物萃取转化合成L-苯基乙酰基甲醇条件的研究[J].食品科技,2010,35(2):2-5.
[11]薛迎迎.响应面法优化微生物萃取转化制备L-苯基乙酰基甲醇的工艺[J].中国药房,2017,28(19):2692-2695.
[2]胡克杰,王跃红,王栋.金银花中绿原酸在体外抗病毒作用的实验研究[J].中医药信息,2010,7(3):27-28.
[3]刘雄,高建德.金银花质量控制、化学成分及药理学研究进展[J].甘肃中医学院学报,2006,23(4):46-48.
[4]国家药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015.
[5]韩飞飞,吴绵斌,孔繁智.天然活性多糖药理作用研究进展[J].西北药学杂志,2006,21(2):91-92.
[6]王宗成,彭延波,盘雪,等.响应面设计优化苎麻叶黄酮提取工艺[J].中国饲料,2016,19(3):12-15.
[7]罗毅,潘细贵,刘刚,等.苯酚-硫酸法测定多糖含量显色方式的优选[J].中国中医药信息杂志,2005,12(1):45-46.
[8]石奇.酶辅助提取金银花中绿原酸与多糖的工艺研究[J].应用化工,2015,44(7):1268-1271.
[9]雷艳萍,石晓峰,刘东彦,等.响应面法优化雪松松针中总木脂素的提取工艺[J].中国药房,2016,27(7):939-942.
[10]薛迎迎,杨汝德,齐瀚实,等.胶束溶液中微生物萃取转化合成L-苯基乙酰基甲醇条件的研究[J].食品科技,2010,35(2):2-5.
[11]薛迎迎.响应面法优化微生物萃取转化制备L-苯基乙酰基甲醇的工艺[J].中国药房,2017,28(19):2692-2695.