间充质干细胞联合石墨烯支架培养治疗膝骨性关节炎的研究进展
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摘要
膝骨性关节炎是困扰中老年人的疾病之一,关节软骨损伤是其病理过程的核心,围绕软骨损伤的病理研究也越来越深入,而现有的治疗方法尚不能从根本上逆转这一过程。近年来随着软骨组织工程技术的发展,特别是间充质干细胞的使用为解决这一困难提供了一条新的途径。同时,石墨烯材料在医学领域的应用也逐渐成为热门,利用石墨烯材料作为支架结构联合间充质干细胞,以此寻求对软骨损伤修复的新方法。本文从膝软骨损伤的机制、间充质干细胞对软骨的修复以及石墨烯支架材料的应用方面进行总结,以期能开拓新的治疗思路。
Knee osteoarthritis is one of diseases that afflict the middle-aged and old people. Articular cartilage injury is the core of its pathological process. The pathological researches around cartilage damage have become more and more indeepth, and the existing treatment cannot fundamentally reverse this process. However, with the development of cartilage tissue engineering technology in recent years, especially the use of mesenchymal stem cells has provided a new way to solve this difficulty. At the same time, the application of graphene materials in the medical field has gradually become popular.The use of graphene material properties as a scaffold structure combined with mesenchymal stem cells to seek a new method for cartilage damage repair, this paper will summarize the mechanism of knee cartilage injury, the repair of cartilage by mesenchymal stem cells and the application of graphene scaffold materials, in order to develop new treatment ideas.
Knee osteoarthritis is one of diseases that afflict the middle-aged and old people. Articular cartilage injury is the core of its pathological process. The pathological researches around cartilage damage have become more and more indeepth, and the existing treatment cannot fundamentally reverse this process. However, with the development of cartilage tissue engineering technology in recent years, especially the use of mesenchymal stem cells has provided a new way to solve this difficulty. At the same time, the application of graphene materials in the medical field has gradually become popular.The use of graphene material properties as a scaffold structure combined with mesenchymal stem cells to seek a new method for cartilage damage repair, this paper will summarize the mechanism of knee cartilage injury, the repair of cartilage by mesenchymal stem cells and the application of graphene scaffold materials, in order to develop new treatment ideas.
引文
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[29]Saxby DJ,Lloyd DG.Osteoarthritis year in review 2016:mechanics[J].Osteoarthritis Cartilage,2017,25(2):190-198.doi:10.1016/j.joca.2016.09.023.
[30]Heckelsmiller DJ,James RM,Baer TE,et al.Changes in joint contact mechanics in a large quadrupedal animal model after partial meniscectomy and a focal cartilage injury[J].J Biomech Eng,2017,139(5):54501.doi:10.1115/1.4036148.
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[2]Trohatou O,Roubelakis MG.Mesenchymal stem/stromal cells in regenerative medicine:past,present,and future[J].Cell Reprogram,2017,19(4):217-224.doi:10.1089/cell.2016.0062.
[3]Shimomura K,Ando W,Moriguchi Y,et al.Next generation mesenchymal stem cell(MSC)-based cartilage repair using scaffold-free tissue engineered constructs generated with synovial mesenchymal stem cells[J].Cartilage,2015,6(2 Suppl):13-29.doi:10.1177/1947603515571002.
[4]Holt BD,Wright ZM,Arnold AM,et al.Graphene oxide as a scaffold for bone regeneration[J].Wiley Interdiscip Rev Nanomed Nanobiotechnol,2017,9(3):e1437.doi:10.1002/wnan.1437.
[5]Wang K,Xu J,Cai J,et al.Serum levels of resistin and interleukin-17 are associated with increased cartilage defectsand bone marrow lesions in patients with knee osteoarthri-tis[J].Modern rheumatology,2017,27(2):339-344.doi:10.1080/14397595.2016.1205777.
[6]Li F,Yao J,Tian H,et al.Correlations between traditional chinese medicine syndromes and IL-1,MMP and TIMP-1 in knee osteoarthritis[J].Int J Clin Exp Med,2017,10(10):14660-14666.
[7]Duro-Castano A,Lim NH,Tranchant I,et al.In vivo imaging of MMP-13 activity using a specific polymer-FRET peptide conjugate detects early osteoarthritis and inhibitor efficacy[J].ADV Funct Mater,2018:1802738.doi:10.1002/adfm.201802738.
[8]李乐,寇久社.三种质量浓度医用臭氧局部注射对兔膝OA行为学及滑膜匀浆中IL-1、TNF-α水平的影响[J].解放军预防医学杂志,2018,36(1):77-79,83.Li L,Kou JS.Effect of local injection with medical ozone of three concentrations on knee osteoarthritis and on levels of IL-1 and TNF-αin new zealand rabbits[J].J Prev Med Chin Peopl Liber Army,2018,36(1):77-79,83.doi:10.13704/j.cnki.jyyx.2018.01.023.
[9]Yamasaki K,Nakasa T,Miyaki S,et al.Expression of microRNA-146a in osteoarthritis cartilage[J].Arthritis Rheum,2009,60(4):1035-1041.doi:10.1002/art.24404.
[10]Jones KJ,Sheppard WL,Arshi A,et al.Articular cartilage lesion characteristic reporting is highly variable in clinical outcomes studies of the knee[J].Cartilage,2018,Feb 1:1947603518756464.doi:10.1177/1947603518756464.[Epub ahead of print].
[11]Zhang X,Wu S,Naccarato T,et al.Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells[J].PLoS One,2017,12(6):e0180138.doi:10.1371/journal.pone.0180138.
[12]Sasaki T,Akagi R,Akatsu Y,et al.The effect of systemic administration of G-CSF on a full-thickness cartilage defect in a rabbit model MSC proliferation as presumed mechanism:G-CSFfor cartilage repair[J].Bone Joint Res,2017,6(3):123-131.doi:10.1302/2046-3758.63.BJR-2016-0083.
[13]Chang YH,Liu HW,Wu KC,et al.Mesenchymal stem cells and their clinical applications in osteoarthritis[J].Cell Transplant,2016,25:937-950.doi:10.3727/096368915X690288.
[14]Yoshimura H,Muneta T,Nimura A,et al.Comparison of rat mesenchymal stem cells derived from bone marrow,synovium,periosteum,adipose tissue,and muscle[J].Cell Tissue Res,2007,327(3):449-462.doi:10.1007/s00441-006-0308-z.
[15]Tao SC,Yuan T,Zhang YL,et al.Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model[J].Theranostics,2017,7(1):180-195.doi:10.7150/thno.17133.
[16]Zhang S,Chuah SJ,Lai RC,et al.MSC exosomes mediate cartilage repair by enhancing proliferation,attenuating apoptosis and modulating immune reactivity[J].Biomaterials,2017,156:16-27.doi:10.1016/j.biomaterials.2017.11.028.
[17]Toh WS,Lai RC,Hui JHP,et al.MSC exosome as a cell-free MSCtherapy for cartilage regeneration:implications for osteoarthritis treatment[C].Semin Cell Dev Biol,2017,67:56-64.doi:10.1016/j.semcdb.2016.11.008.
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[19]Paek HJ,Kim C,Tuan RS.Stem cell-based repair and regeneration of articular cartilage[J].J Stem Cell Res Ther,2017,2(6):177-182.doi:10.15406/jsrt.2017.02.00082.
[20]Gobbi A,Espregueira-Mendes J,Lane JG,et al.Bio-Orthopaedics:A new approach[M].Springer:Berlin/Heidelberg,Germnay,2017:87-103.ISBN:978-3-662-54180-7.
[21]Sawatjui N,Limpaiboon T,Schrobback K,et al.Biomimetic scaffolds and dynamic compression enhance the properties of chondrocyte and MSC-based tissue-engineered cartilage[J].JTissue Eng Regen Med,2018,12(5):1220-1229.doi:10.1002/term.2653.
[22]Peruzynska M,Cendrowski K,Barylak M,et al.Comparative in vitro study of single and four layer graphene oxide nanoflakeCytotoxicity and cellular uptake[J].Toxicol In Vitro,2017,41:205-213.doi:10.1016/j.tiv.2017.03.005.
[23]Tonelli FM,Goulart VA,Gomes KN,et al.Graphene based nanomaterials:biological and medical applications and toxicity[J].Nanomedicine,2015,10(15):2423-2450.doi:10.2217/nnm.15.65.
[24]魏丽君,曹均凯,李俊杰,等.低聚乙二醇富马酸酯/氧化石墨烯复合水凝胶对大鼠骨髓间充质干细胞成骨分化的调控作用[J].解放军医学院学报,2016,37(6):611-616,633.Wei LJ,Cao JK,Li JJ,et al.Effects of oligo[poly(ethylene glycol)fumarate]/graphene oxide on osteogenic differentiation of bone marrow mesenchymal stem cells in rats[J].Academic Journal of Chinese PLA Medical School,2016,37(6):611-616,633.doi:10.3969/j.issn.2095-5227.2016.06.023.
[25]Wang W,Caetano GF,Chiang WH,et al.Morphological,mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration[J].Int J Bioprint,2016,2(2):204-213.doi:10.18063/IJB.2016.02.009.
[26]Shen H,Lin H,Sun AX,et al.Chondroinductive factor free chondrogenic differentiation of human mesenchymal stem cells in graphene oxide-incorporated hydrogels[J].J Mater Chem B,2018,6(6):908-917.doi:10.1039/C7TB02172K.
[27]Zhou X,Nowicki M,Cui H,et al.3D bioprinted graphene oxideincorporated matrix for promoting chondrogenic differentiation of human bone marrow mesenchymal stem cells[J].Carbon,2017,116:615-624.doi:10.1016/j.carbon.2017.02.049.
[28]Nalvuran H,El?in AE,El?in YM.Nanofibrous silk fibroin/reduced graphene oxide scaffolds for tissue engineering and cell culture applications[J].Int J Biol Macromol,2018,114(15):77-84.doi:10.1016/j.ijbiomac.2018.03.072.
[29]Saxby DJ,Lloyd DG.Osteoarthritis year in review 2016:mechanics[J].Osteoarthritis Cartilage,2017,25(2):190-198.doi:10.1016/j.joca.2016.09.023.
[30]Heckelsmiller DJ,James RM,Baer TE,et al.Changes in joint contact mechanics in a large quadrupedal animal model after partial meniscectomy and a focal cartilage injury[J].J Biomech Eng,2017,139(5):54501.doi:10.1115/1.4036148.
[31]Li D,Liu T,Yu X,et al.Fabrication of graphene-biomacromolecule hybrid materials for tissue engineering application[J].Polym Chem-Uk,2017,8(30):4309-4321.doi:10.1039/C7PY00935F.2018-09-112018-10-30