银杏黄酮对非酒精性脂肪肝小鼠的影响及其机制
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摘要
目的观察银杏黄酮对非酒精性脂肪肝(NAFLD)小鼠的影响及其机制。方法 60只健康雄性ICR小鼠以连续饲喂高脂饲料法构建NAFLD小鼠模型并随机分为模型组及低、中、高剂量实验组,各15只,另选15只健康雄性ICR小鼠常规饲养并作为对照组。造模期间,低、中、高剂量实验组予以灌胃银杏黄酮75,150,300 mg·kg~(-1),模型组及对照组灌胃等量0. 9%NaCl,每日1次,共8周。用全自动生化分析仪检测血清肝功能指标含量,用ELISA法检测肝脏脂代谢指标,用蛋白免疫印迹法检测肝脏组织核转录因子κBp65(NF-κBp65)蛋白表达。结果对照组,模型组及低、中、高剂量实验组血清谷丙转氨酶(GPT)分别为(41. 25±5. 21),(82. 19±5. 13),(76. 53±4. 89),(60. 02±4. 83),(53. 89±4. 52) U·L~(-1);谷草转氨酶(GOT)水平分别为(101. 33±18. 76),(179. 84±18. 39),(161. 28±16. 48),(149. 06±16. 23),(132. 17±16. 05)U·L~(-1);肝脏组织总胆固醇(TC)分别为(1. 31±0. 24),(3. 96±0. 22),(3. 24±0. 21),(2. 90±0. 19),(2. 54±0. 16) mmol·g~(-1);甘油三酯(TG)分别为(1. 39±0. 23),(2. 71±0. 21),(2. 32±0. 20),(1. 94±0. 18),(1. 76±0. 16)mmol·g~(-1);肝脏组织NF-κBp65蛋白相对表达量分别为0. 16±0. 08,0. 89±0. 12,0. 82±0. 09,0. 31±0. 07,0. 25±0. 04。模型组分别与对照组和低、中、高剂量实验组比较,差异均有统计学意义(均P <0. 05)。结论银杏黄酮可有效抑制NAFLD小鼠肝脏脂肪变性,恢复肝脏脂代谢及肝功能,其作用机制可能与下调NF-κBp65蛋白有关。
Objective To observe the effect of ginkgo flavonoids on mice with non-alcoholic fatty liver disease( NAFLD). Methods A total of 60 ICR male mice were given high fat diet to induce NAFLD,and then assigned to model group and experimental-L/-M/-H groups,with 15 cases in each group. Fifteen ICR male mice were assigned to control group to receive regular diet. The experimental-L/-M/-H groups were given oral administration of 75,150,300 mg·kg~(-1) ginkgo flavonoids once daily for 8 weeks,while the model group and control group received equal amount 0. 9% NaCl. At 8 weeks after treatment,the liver lipid metabolism indexes and serum liver function indexes content were detected; the expression of liver tissue nuclear transcription factor-κB p65( NF-κBp65) protein of mice were detected by western blot.Results The serum glutamic pyruvic transaminase( GPT) levels in control group,model group and experimental-L/-M/-H groups were( 41. 25 ± 5. 21),( 82. 19 ± 5. 13),( 76. 53 ± 4. 89),( 60. 02 ± 4. 83),( 53. 89 ± 4. 52) U·L~(-1),respectively; the glutamic oxaloacetic transaminase( GOT) levels were( 101. 33 ± 18. 76),( 179. 84 ± 18. 39),( 161. 28 ± 16. 48),( 149. 06 ± 16. 23),( 132. 17 ± 16. 05) U·L~(-1),respectively; the liver tissue total cholesterol( TC) contents were( 1. 31 ± 0. 24),( 3. 96 ± 0. 22),( 3. 24 ± 0. 21),( 2. 90 ± 0. 19),( 2. 54 ± 0. 16) mmol·g~(-1),respectively; the triglyceride( TG) contents were( 1. 39 ± 0. 23),( 2. 71 ± 0. 21),( 2. 32 ± 0. 20),( 1. 94 ± 0. 18),( 1. 76 ± 0. 16)mmol·g~(-1),respectively; the relative expression of live tissue NF-κBp65 protein were 0. 16 ± 0. 08,0. 89 ± 0. 12,0. 82 ± 0. 09,0. 31 ± 0. 07,0. 25 ± 0. 04,respectively. There were statistically significant differences between the model group and the control group/experimental-L/-M/-H group,respectively( P < 0. 05). Conclusion Ginkgo flavonoids can inhibit NAFLD mice liver fat degeneration,recover the liver lipid metabolism and liver function,the action mechanism maybe related to the down-regulation of the expression liver tissue NF-κBp65 protein.
Objective To observe the effect of ginkgo flavonoids on mice with non-alcoholic fatty liver disease( NAFLD). Methods A total of 60 ICR male mice were given high fat diet to induce NAFLD,and then assigned to model group and experimental-L/-M/-H groups,with 15 cases in each group. Fifteen ICR male mice were assigned to control group to receive regular diet. The experimental-L/-M/-H groups were given oral administration of 75,150,300 mg·kg~(-1) ginkgo flavonoids once daily for 8 weeks,while the model group and control group received equal amount 0. 9% NaCl. At 8 weeks after treatment,the liver lipid metabolism indexes and serum liver function indexes content were detected; the expression of liver tissue nuclear transcription factor-κB p65( NF-κBp65) protein of mice were detected by western blot.Results The serum glutamic pyruvic transaminase( GPT) levels in control group,model group and experimental-L/-M/-H groups were( 41. 25 ± 5. 21),( 82. 19 ± 5. 13),( 76. 53 ± 4. 89),( 60. 02 ± 4. 83),( 53. 89 ± 4. 52) U·L~(-1),respectively; the glutamic oxaloacetic transaminase( GOT) levels were( 101. 33 ± 18. 76),( 179. 84 ± 18. 39),( 161. 28 ± 16. 48),( 149. 06 ± 16. 23),( 132. 17 ± 16. 05) U·L~(-1),respectively; the liver tissue total cholesterol( TC) contents were( 1. 31 ± 0. 24),( 3. 96 ± 0. 22),( 3. 24 ± 0. 21),( 2. 90 ± 0. 19),( 2. 54 ± 0. 16) mmol·g~(-1),respectively; the triglyceride( TG) contents were( 1. 39 ± 0. 23),( 2. 71 ± 0. 21),( 2. 32 ± 0. 20),( 1. 94 ± 0. 18),( 1. 76 ± 0. 16)mmol·g~(-1),respectively; the relative expression of live tissue NF-κBp65 protein were 0. 16 ± 0. 08,0. 89 ± 0. 12,0. 82 ± 0. 09,0. 31 ± 0. 07,0. 25 ± 0. 04,respectively. There were statistically significant differences between the model group and the control group/experimental-L/-M/-H group,respectively( P < 0. 05). Conclusion Ginkgo flavonoids can inhibit NAFLD mice liver fat degeneration,recover the liver lipid metabolism and liver function,the action mechanism maybe related to the down-regulation of the expression liver tissue NF-κBp65 protein.
引文
[1]曾斌芳,杨旋,王洁.中医药治疗非酒精性脂肪肝的临床进展[J].新疆医科大学学报,2018,41(2):144-146.
[2]涂清波,孙云,许婷,等.银杏双黄酮药理作用的研究进展[J].山东医药,2018,58(19):112-114.
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[5]潘磊,崔荣岗,赵保辉,等.蛤蚧肽溶液对非酒精性脂肪肝小鼠脂代谢、肝功能及炎性因子的影响[J].陕西中医,2016,37(3):376-379.
[6]高敬国.熊果酸对非酒精性脂肪肝大鼠肝脏的保护作用及机制[J].中药药理与临床,2016,32(2):27-31.
[7]于俊杰,詹晓蓉,王晓辰,等. MicroRNA-185改善非酒精性脂肪肝小鼠胰岛素敏感性并调节脂代谢基因的表达[J].现代生物医学进展,2018,18(8):1428-1430.
[8]杨莉,郭云,郑浩杰,等.乌丹降脂胶囊对NAFLD大鼠肝脏NF-κB表达及血清生化指标的影响[J].现代中西医结合杂志,2017,26(1):7-9.
[2]涂清波,孙云,许婷,等.银杏双黄酮药理作用的研究进展[J].山东医药,2018,58(19):112-114.
[3]罗云,卢珊,周平,等.木犀草素改善高脂诱导的ApoE-/-小鼠非酒精性脂肪肝及动脉粥样硬化作用研究[J].世界中医药,2015,10(8):1144-1147.
[4]覃俊媛,李梦婷,杨雪,等.赶黄草总黄酮对非酒精性脂肪肝小鼠肝功能和脂质代谢的影响[J].成都中医药大学学报,2017,40(4):33-36.
[5]潘磊,崔荣岗,赵保辉,等.蛤蚧肽溶液对非酒精性脂肪肝小鼠脂代谢、肝功能及炎性因子的影响[J].陕西中医,2016,37(3):376-379.
[6]高敬国.熊果酸对非酒精性脂肪肝大鼠肝脏的保护作用及机制[J].中药药理与临床,2016,32(2):27-31.
[7]于俊杰,詹晓蓉,王晓辰,等. MicroRNA-185改善非酒精性脂肪肝小鼠胰岛素敏感性并调节脂代谢基因的表达[J].现代生物医学进展,2018,18(8):1428-1430.
[8]杨莉,郭云,郑浩杰,等.乌丹降脂胶囊对NAFLD大鼠肝脏NF-κB表达及血清生化指标的影响[J].现代中西医结合杂志,2017,26(1):7-9.