抗坏血酸引发对Na_2SO_4胁迫下燕麦种子活力的影响
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摘要
[目的]探讨Na_2SO_4胁迫下抗坏血酸(ASC)引发对燕麦种子活力的影响。[方法]通过以0、0.5、1.0、1.5及2.0 mmol·L~(-1)的ASC对燕麦种子进行引发,然后在浓度为0、50和100 mmol·L~(-1)的Na_2SO_4胁迫下发芽,并分析其发芽率、发芽指数、平均发芽时间及幼苗活力指数的变化,以探讨外源ASC引发对Na_2SO_4胁迫下燕麦种子活力的影响。[结果]Na_2SO_4胁迫会降低燕麦种子发芽率、发芽指数和幼苗活力指数,并增加其平均发芽时间。然而,ASC引发能提高其发芽率、发芽指数和幼苗活力指数。[结论]ASC引发能够提高Na_2SO_4胁迫燕麦种子的活力,而且与ASC和Na_2SO_4浓度密切相关,试验中以1.5 mmol·L~(-1)的ASC浓度引发效果最好,尤其在100 mmol·L~(-1)的Na_2SO_4胁迫下。因此,在实践中应根据燕麦种子本身的活力水平和盐胁迫程度慎重地采用ASC引发。
[Objective]This study was undertaken to explore the effect of exogenous ascorbic acid(ASC) on the vigor of oat seeds under Na_2SO_4 stress. [Methods] Oat seeds were primed with ASC at different concentrations(0, 0.5, 1.0, 1.5, and 2.0 mmol·L~(-1)), and germinated under Na_2SO_4 stress at three different concentrations(0, 50, and 100 mmol·L~(-1)).The alteration ofseed germinationratio, germination index, mean germination time, and seedling vigor index was analyzed. [Results] Study results indicated that Na_2SO_4 stressproduced inhibitory activity on seed germinationrate, germination index, and seedling vigor index of oat seeds with extendedaverage germination time. However, these negative impacts were compensated by ASC priming at certain concentration. The vigor of oat seeds was improved by ASC priming under Na_2SO_4 stress, and these effects were closely related to the concentration of both ASC and Na_2SO_4. This study determined that the highest growth rate and vigor were reached out at the ASC concentration of 1.5 mmol·L~(-1)coupled with Na_2SO_4 stress at 100 mmol·L~(-1). [Conclusion] ASC priming can improve seed vigor, but should be used with caution according to the seed vigor of oat seeds and the degree of salt stress.
[Objective]This study was undertaken to explore the effect of exogenous ascorbic acid(ASC) on the vigor of oat seeds under Na_2SO_4 stress. [Methods] Oat seeds were primed with ASC at different concentrations(0, 0.5, 1.0, 1.5, and 2.0 mmol·L~(-1)), and germinated under Na_2SO_4 stress at three different concentrations(0, 50, and 100 mmol·L~(-1)).The alteration ofseed germinationratio, germination index, mean germination time, and seedling vigor index was analyzed. [Results] Study results indicated that Na_2SO_4 stressproduced inhibitory activity on seed germinationrate, germination index, and seedling vigor index of oat seeds with extendedaverage germination time. However, these negative impacts were compensated by ASC priming at certain concentration. The vigor of oat seeds was improved by ASC priming under Na_2SO_4 stress, and these effects were closely related to the concentration of both ASC and Na_2SO_4. This study determined that the highest growth rate and vigor were reached out at the ASC concentration of 1.5 mmol·L~(-1)coupled with Na_2SO_4 stress at 100 mmol·L~(-1). [Conclusion] ASC priming can improve seed vigor, but should be used with caution according to the seed vigor of oat seeds and the degree of salt stress.
引文
[1]杜利霞,董宽虎,夏方山,等.盐胁迫对新麦草种子萌发特性与生理特性的影响[J].草地学报,2009,17(6):789-794.
[2]夏方山,毛培胜,闫慧芳,等.水杨酸对植物种子及幼苗抗逆性的影响[J].草业科学,2014,31(7):1367-1373.
[3]高战武,蔺吉祥,邵帅,等.复合盐碱胁迫对燕麦种子发芽的影响[J].草业科学,2014,31(3):451-456.
[4]王霞霞,李龑,唐杰伟,等.盐胁迫对燕麦种子萌发期蛋白质组影响的研究[J].华北农学报,2015,30(3):48-53.
[5]Xia F S, Chen L L, Yan H F, et al. Antioxidant and ultrastructural responses to priming with PEG in aged, ultra-dry oat seed [J]. Seed Science and Technology, 2016, 44(3): 1-13.
[6]Bewley J D, Bradford K J, Hilhorst H W M, et al. Seeds: Physiology of development, germination and dormancy [M]. New York: Springer Press, 2013: 341-374.
[7]Beltagi M S. Exogenous ascorbic acid (vitamin C) induced anabolic changes for salt tolerance in chick pea (Cicer arietinum L.) plants [J]. African Journal of Plant Science, 2008, 2(10): 118-123.
[8]Ejaz B, Sajid Z A, Aftab F. Effect of exogenous application of ascorbic acid on antioxidant enzyme activities, proline contents, and growth parameters of Saccharum spp. hybrid cv. HSF-240 under salt stress [J]. Turkish Journal of Biology, 2012, 36(6): 630-640.
[9]董秋丽,夏方山,丁荷星,等.外源抗坏血酸引发促进Na2SO4胁迫下燕麦幼苗的生长[J].草业科学,2018,35(3):558-565.
[10]Saeidi-Sar S, Abbaspour H, Afshari H, et al. Effects of ascorbic acid and gibberellin A3 on alleviation of salt stress in common bean (Phaseolus vulgaris L.) seedlings [J]. Acta Physiologiae Plantarum, 2013, 35(3): 667.
[11]常云霞,徐克东,周琳,等.抗坏血酸对盐胁迫下小麦幼苗生长抑制的缓解效应[J].麦类作物学报,2013,33(1):151-155.
[12]Khan A, Iqbal I, Shah A, et al. Alleviation of adverse effects of salt stress in brassica (Brassica campestris) by pre-sowing seed treatment with ascorbic acid [J]. American-Eurasian Journal of Agricultural and Environmental Science, 2010, 7(5): 557-560.
[13]董秋丽,夏方山,李晓禹,等.抗坏血酸引发对NaCl胁迫燕麦种子活力的影响[J].草业学报,2018,27(4):202-208.
[14]郭红媛,贾举庆,吕晋慧,等.燕麦属种质资源遗传多样性及遗传演化关系ISSR分析[J].草地学报,2014,22(2):344-351.
[15]刘凤歧,刘杰淋,朱瑞芬,等.4种燕麦对NaCl胁迫的生理响应及耐盐性评价[J].草业学报,2015,24(1):183-189.
[16]郑曦,魏臻武,武自念,等.不同燕麦品种(系)在扬州地区的适应性评价[J].草地学报,2013,21(2):272-279.
[17]Shewry P R, Piironen V, Lampi A M, et al. Phytochemical and fiber components in oat varieties in the HEALTHGRAIN diversity screen [J]. Journal of Agricultural and Food Chemistry, 2008, 56(21): 9777-9784.
[18]夏方山,王婷婷,董秋丽,等.外源H2O2处理对燕麦种子活力的影响[J].草业学报,2018,27(3):201-207.
[19]刘建新,王金成,贾海燕.燕麦幼苗对盐胁迫和碱胁迫的生理响应差异[J].水土保持学报,2015,29(5):331-336.
[20]武英俊,刘景辉,李倩.盐胁迫对燕麦幼苗生长,K+、Na+吸收和光合性能的影响[J].西北农业学报,2010,19(2):100-105.
[21]夏方山,李晓禹,董秋丽,等.抗坏血酸引发对NaCl胁迫燕麦幼苗生长的影响[J].山西农业大学学报(自然科学版),2017,37(11):761-766.
[22]ISTA. International Rules for Seed Testing: The germination test [M]. Zurich: International Seed Testing Association, 2015: 25.
[23]Abdul Baki A A, Anderson J D. Vigor determination in soybean seed by multiple criteria 1 [J]. Crop Science, 1973, 13(6): 630-633.
[24]Ellis R H, Osei-Bonsu K, Roberts E H. The influence of genotype, temperature and moisture on seed longevity in chickpea, cowpea and soyabean [J]. Annals of Botany, 1982, 50(1): 69-82.
[25]卢培娜,刘景辉,李倩,等.盐碱地不同燕麦品种的品质及产量比较[J].麦类作物学报,2016,36(11):1510-1516.
[26]刘拥海,俞乐,王若仲,等.抗坏血酸对植物生长发育的作用及其缺失突变体的研究进展[J].植物生理学报,2011,47(9):847-854.
[27]Liu Y H, Yu L, Wang R Z. Level of ascorbic acid in transgenic rice for L-galactono-1, 4-lactone dehydrogenase overexpressing or suppressed is associated with plant growth and seed set [J]. Acta Physiologiae Plantarum, 2011, 33(4): 1353-1363.
[28]Shin R, Schachtman D P. Hydrogen peroxide mediates plant root cell response to nutrient deprivation [J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(23): 8827-8832.
[29]张启雷,刘强,高辉,等.内源抗坏血酸对水稻种子萌发及幼苗生长的影响[J].热带亚热带植物学报,2016,24(3):273-279.
[30]常云霞,徐克东,刘彬,等.外源抗坏血酸对镉毒害小麦幼苗的缓解作用[J].麦类作物学报,2017,37(2):246-252.
[31]Gallie D R. The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth [J]. Journal of Experiment Botany, 2013, 64(2): 433-443.
[32]范美华,张义鑫,石戈,等.外源抗坏血酸对油菜种子在海水胁迫下萌发生长的影响[J].中国油料作物学报,2009,31(1):34-38.
[33]刘艳军,范晶,韩学珅,等.外源维生素对高NaCl胁迫下紫花苜蓿种子萌发及恢复性的影响[J].中国农学通报,2015,31(26):12-27.
[34]江绪文,李贺勤,王建华.盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应[J]. 植物生理学报,2015,51(2):166-170.
[35]石永春,杨永银,薛瑞丽,等.植物中抗坏血酸的生物学功能研究进展[J].植物生理学报,2015,51(1):1-8.
[2]夏方山,毛培胜,闫慧芳,等.水杨酸对植物种子及幼苗抗逆性的影响[J].草业科学,2014,31(7):1367-1373.
[3]高战武,蔺吉祥,邵帅,等.复合盐碱胁迫对燕麦种子发芽的影响[J].草业科学,2014,31(3):451-456.
[4]王霞霞,李龑,唐杰伟,等.盐胁迫对燕麦种子萌发期蛋白质组影响的研究[J].华北农学报,2015,30(3):48-53.
[5]Xia F S, Chen L L, Yan H F, et al. Antioxidant and ultrastructural responses to priming with PEG in aged, ultra-dry oat seed [J]. Seed Science and Technology, 2016, 44(3): 1-13.
[6]Bewley J D, Bradford K J, Hilhorst H W M, et al. Seeds: Physiology of development, germination and dormancy [M]. New York: Springer Press, 2013: 341-374.
[7]Beltagi M S. Exogenous ascorbic acid (vitamin C) induced anabolic changes for salt tolerance in chick pea (Cicer arietinum L.) plants [J]. African Journal of Plant Science, 2008, 2(10): 118-123.
[8]Ejaz B, Sajid Z A, Aftab F. Effect of exogenous application of ascorbic acid on antioxidant enzyme activities, proline contents, and growth parameters of Saccharum spp. hybrid cv. HSF-240 under salt stress [J]. Turkish Journal of Biology, 2012, 36(6): 630-640.
[9]董秋丽,夏方山,丁荷星,等.外源抗坏血酸引发促进Na2SO4胁迫下燕麦幼苗的生长[J].草业科学,2018,35(3):558-565.
[10]Saeidi-Sar S, Abbaspour H, Afshari H, et al. Effects of ascorbic acid and gibberellin A3 on alleviation of salt stress in common bean (Phaseolus vulgaris L.) seedlings [J]. Acta Physiologiae Plantarum, 2013, 35(3): 667.
[11]常云霞,徐克东,周琳,等.抗坏血酸对盐胁迫下小麦幼苗生长抑制的缓解效应[J].麦类作物学报,2013,33(1):151-155.
[12]Khan A, Iqbal I, Shah A, et al. Alleviation of adverse effects of salt stress in brassica (Brassica campestris) by pre-sowing seed treatment with ascorbic acid [J]. American-Eurasian Journal of Agricultural and Environmental Science, 2010, 7(5): 557-560.
[13]董秋丽,夏方山,李晓禹,等.抗坏血酸引发对NaCl胁迫燕麦种子活力的影响[J].草业学报,2018,27(4):202-208.
[14]郭红媛,贾举庆,吕晋慧,等.燕麦属种质资源遗传多样性及遗传演化关系ISSR分析[J].草地学报,2014,22(2):344-351.
[15]刘凤歧,刘杰淋,朱瑞芬,等.4种燕麦对NaCl胁迫的生理响应及耐盐性评价[J].草业学报,2015,24(1):183-189.
[16]郑曦,魏臻武,武自念,等.不同燕麦品种(系)在扬州地区的适应性评价[J].草地学报,2013,21(2):272-279.
[17]Shewry P R, Piironen V, Lampi A M, et al. Phytochemical and fiber components in oat varieties in the HEALTHGRAIN diversity screen [J]. Journal of Agricultural and Food Chemistry, 2008, 56(21): 9777-9784.
[18]夏方山,王婷婷,董秋丽,等.外源H2O2处理对燕麦种子活力的影响[J].草业学报,2018,27(3):201-207.
[19]刘建新,王金成,贾海燕.燕麦幼苗对盐胁迫和碱胁迫的生理响应差异[J].水土保持学报,2015,29(5):331-336.
[20]武英俊,刘景辉,李倩.盐胁迫对燕麦幼苗生长,K+、Na+吸收和光合性能的影响[J].西北农业学报,2010,19(2):100-105.
[21]夏方山,李晓禹,董秋丽,等.抗坏血酸引发对NaCl胁迫燕麦幼苗生长的影响[J].山西农业大学学报(自然科学版),2017,37(11):761-766.
[22]ISTA. International Rules for Seed Testing: The germination test [M]. Zurich: International Seed Testing Association, 2015: 25.
[23]Abdul Baki A A, Anderson J D. Vigor determination in soybean seed by multiple criteria 1 [J]. Crop Science, 1973, 13(6): 630-633.
[24]Ellis R H, Osei-Bonsu K, Roberts E H. The influence of genotype, temperature and moisture on seed longevity in chickpea, cowpea and soyabean [J]. Annals of Botany, 1982, 50(1): 69-82.
[25]卢培娜,刘景辉,李倩,等.盐碱地不同燕麦品种的品质及产量比较[J].麦类作物学报,2016,36(11):1510-1516.
[26]刘拥海,俞乐,王若仲,等.抗坏血酸对植物生长发育的作用及其缺失突变体的研究进展[J].植物生理学报,2011,47(9):847-854.
[27]Liu Y H, Yu L, Wang R Z. Level of ascorbic acid in transgenic rice for L-galactono-1, 4-lactone dehydrogenase overexpressing or suppressed is associated with plant growth and seed set [J]. Acta Physiologiae Plantarum, 2011, 33(4): 1353-1363.
[28]Shin R, Schachtman D P. Hydrogen peroxide mediates plant root cell response to nutrient deprivation [J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(23): 8827-8832.
[29]张启雷,刘强,高辉,等.内源抗坏血酸对水稻种子萌发及幼苗生长的影响[J].热带亚热带植物学报,2016,24(3):273-279.
[30]常云霞,徐克东,刘彬,等.外源抗坏血酸对镉毒害小麦幼苗的缓解作用[J].麦类作物学报,2017,37(2):246-252.
[31]Gallie D R. The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth [J]. Journal of Experiment Botany, 2013, 64(2): 433-443.
[32]范美华,张义鑫,石戈,等.外源抗坏血酸对油菜种子在海水胁迫下萌发生长的影响[J].中国油料作物学报,2009,31(1):34-38.
[33]刘艳军,范晶,韩学珅,等.外源维生素对高NaCl胁迫下紫花苜蓿种子萌发及恢复性的影响[J].中国农学通报,2015,31(26):12-27.
[34]江绪文,李贺勤,王建华.盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应[J]. 植物生理学报,2015,51(2):166-170.
[35]石永春,杨永银,薛瑞丽,等.植物中抗坏血酸的生物学功能研究进展[J].植物生理学报,2015,51(1):1-8.