穿心莲内酯对人宫颈癌SiHa细胞增殖、凋亡及细胞周期的影响
摘要
目的研究穿心莲内酯对人宫颈癌Si Ha细胞增殖、凋亡、细胞周期的影响及潜在机制。方法采用0、20、40和80μmol·L~(-1)的穿心莲内酯处理Si Ha细胞,其中0μmol·L~(-1)的穿心莲内酯组为对照组;通过MTS法检测细胞增殖能力,Annexin V-FITC/PI双染法检测细胞凋亡率,分光光度法检测Caspase-3活化率,PI单染法检测细胞周期分布,蛋白免疫印迹法检测Wnt/β-catenin信号通路蛋白表达水平。结果与对照组相比,20μmol·L~(-1)穿心莲内酯处理SiHa细胞24 h、48 h、72 h和96 h及40、80μmol·L~(-1)穿心莲内酯处理24 h、48 h、72 h和96 h后的存活率均明显降低(P<0. 05);与对照组相比,20、40、80μmol·L~(-1)穿心莲内酯组细胞凋亡率、Caspase-3活化率及G0/G1期细胞比例升高,而活化的β-catenin、Cyclin D1及C-myc表达水平降低(P<0. 05)。结论穿心莲内酯具有抑制人宫颈癌SiHa细胞增殖、促进凋亡及细胞周期的阻滞作用,该作用与Wnt/β-catenin信号通路的激活受阻有关。
引文
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[2]Sardain H,Lavoue V,Redpath M,et al.Curative pelvic exenteration for recurrent cervical carcinoma in the era of concurrent chemotherapy and radiation therapy.A systematic review[J].Eur J Surg Oncol,2015,41(8):975-85.
[3]李海霞,张伟.红景天多糖对宫颈癌U14模型小鼠的治疗作用及机制的实验研究[J].中药材,2017,40(6):1453-1456.
[4]王东辉,闻春艳.人参皂苷Rg3对宫颈癌荷瘤小鼠治疗作用的实验研究[J].中国妇幼保健,2016,31(19):4046-4048.
[5]杨静.穿心莲内酯的研究进展[J].中草药,2009,40(7):1168-1170.
[6]亓翠玲,王丽京,周鑫磊.穿心莲内酯抗肿瘤作用机制的研究进展[J].中国中药杂志,2007,13(20):2095-2097.
[7]毕然,杜玉君,魏伟,等.穿心莲内酯对人肾细胞癌786-0细胞增殖、迁移和凋亡的影响[J].吉林大学学报:医学版,2018,44(1):18-23,205.
[8]殷文静,赵君,陈文芬,等.穿心莲内酯诱导宫颈癌He La细胞凋亡的作用及其机制[J].中国妇幼保健,2013,28(25):4220-4223.
[9]Kishore V,Yarla NS,Bishayee A,et al.Multi-targeting Andrographolide and its Natural Analogs as Potential Therapeutic Agents[J].Curr Top Med Chem,2017,17(8):845-857.
[10]Monger A,Boonmuen N,Suksen K,et al.Inhibition of Topoisomerase IIαand Induction of Apoptosis in Gastric Cancer Cells by19-Triisopropyl Andrographolide[J].Asian Pac J Cancer Prev,2017,18(10):2845-2851.
[11]Zhang R,Zhao J,Xu J,et al.Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF-κB/MMP-9 signaling pathway[J].Oncol Lett,2017,14(4):4305-4310.
[12]Guo H,Zhang Z,Su Z,et al.Enhanced anti-tumor activity and reduced toxicity by combination andrographolide and bleomycin in ascitic tumor-bearing mice[J].Eur J Pharmacol,2016,776(1):52-63.
[13]魏强,李静,刘艺,等.MAPK信号转导通路在人参多糖诱导白血病K562细胞凋亡中的作用[J].中草药,2013,44(2):193-198.
[14]葛宇清,杨波,程汝滨,等.隐丹参酮对K562细胞凋亡的影响及其机制[J].中草药,2013,44(22):3188-3194.
[15]Wang Y,Chai Z,Wang M,et al.COPB2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle-related proteins[J].Exp Ther Med,2018,15(1):777-784.
[16]Huang J,Chen S,Shi Y,et al.Epigallocatechin gallate from green tea exhibits potent anticancer effects in A-549 non-small lung cancer cells by inducing apoptosis,cell cycle arrest and inhibition of cell migration[J].J BUON,2017,22(6):1422-1427.
[17]高志强,程会云,李同源,等.知母皂苷AⅢ对白血病细胞HL-60增殖、凋亡和细胞周期的影响及机制探讨[J].中成药,2017,39(7):1487-1490.
[18]Skah S,Uchuya-Castillo J,Sirakov M,et al.The thyroid hormone nuclear receptors and the Wnt/β-catenin pathway:An intriguing liaison[J].Dev Biol,2017,422(2):71-82.
[19]Mc Cubrey JA,Rakus D,Gizak A,et al.Effects of mutations in Wnt/β-catenin,hedgehog,Notch and PI3K pathways on GSK-3activity-Diverse effects on cell growth,metabolism and cancer[J].Biochim Biophys Acta,2016,1863(12):2942-2976.
[20]高启龙,朱亚楠,石变,等.青龙衣含药血清抑制胃癌SGC-7901细胞生长和诱导凋亡[J].中国实验方剂学杂志,2017,23(13):111-115.