天冬氨酸酶基因工程菌的研究
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摘要
为了解天冬氨酸酶的性质和作用,从天冬氨酸酶性质、天冬氨酸酶作用机理、天冬氨酸酶固定化和天冬氨酸酶应用四个方面对天冬氨酸酶研究工作进行归纳总结。研究结果表明,天冬氨酸酶能将反丁烯二酸与氨水催化合成L-天冬氨酸,天冬氨酸酶是工业应用中具有重要作用酶。能够对L-天冬氨酸酶运用于医药、食品、化工等行业提供一定的依据。
This paper summarizes the research work on aspartase including aspartase properties, aspartase mechanism, aspartase immobilization and aspartase application to understand the nature and function of aspartase. It can synthesize L-aspartic acid with ammonia by aspartase. Aspartase is an important enzyme in industrial application. L-aspartic acid is widely used in pharmaceutical, food and chemical industries.
This paper summarizes the research work on aspartase including aspartase properties, aspartase mechanism, aspartase immobilization and aspartase application to understand the nature and function of aspartase. It can synthesize L-aspartic acid with ammonia by aspartase. Aspartase is an important enzyme in industrial application. L-aspartic acid is widely used in pharmaceutical, food and chemical industries.
引文
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[2] Takagi JS,Tokushige M,Shimura Y.Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.Journal of Biochemistry,1986,100(3):697-705.
[3] 张红缨,李正强,张今,等.酶法体外建立天冬氨酸酶基因的随机突变库[J].科学通报.1991,19(16):1500-1502.
[4] 林琳,丁文勇,张今.金属离子、蔗糖对天冬氨酸酶活性的影响及其活性部位的分析[J].药物生物技术.1998,5(3):149-152.
[5] Murase S,Yumoto N.Characterization of three types of aspartase activated by site-directed mutagenesis,limited proteolysis,and acetylationl[J].The Journal of Biochemistry,1993,114(5):735-739.
[6] 马林,于振宝,程玉华,等.疏水吸附固定化天冬氨酸酶及其性质的研究[J].生物化学杂志.1991,7(6):671-678.
[7] 周华,杨正景,欧阳平凯.固定化细胞法制备L-天冬氨酸中甲壳素固定化条件的确定[J].南京化工大学学报(自然科学版).1999,21(2):68-70.
[8] 徐礼生,高贵珍,赵亮,等.天冬氨酸酶基因工程菌固定化细胞制备L-天冬氨酸合成条件优化[J].精细化工.2016,33(3):302-306.
[9] 张红缨,李正强,张今,等.酶法体外建立天冬氨酸酶基因的随机突变库[J].科学通报.1991,19(16):1500-1502.
[10] 廖峰,孙小梅,李步海.破壁方法对大肠杆菌AS1.881 中天冬氨酸酶的影响研究[J].氨基酸和生物资源.2004,26(1):29-31.
[11] 张玉彬,王旻,吴梧桐.固定化细胞联产L-苹果酸和L-天冬氨酸的研究[J].中国药科大学学报.1996,27(8):500-503.
[12] 唐芳,谭天伟,王芳.L-天冬氨酸转化菌发酵条件的优化[J].北京化工大学学报.2003,30(5):21-24.
[13] YukawaH,YamadaS,NaraT,et al.Production of L- aspartic acid by reusing cells industrialuse of ethanol utilizing microorganisms[J].Agric Chem Soc Japan.1985,59(1):31-37.
[14] Singh R S,Yadav M.Single-step purification and characterization of recombinant aspartase of Aeromonas media NFB-5[J].Applied Biochemistry & Biotechnology.2012,167(5):991-1001.
[2] Takagi JS,Tokushige M,Shimura Y.Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.Journal of Biochemistry,1986,100(3):697-705.
[3] 张红缨,李正强,张今,等.酶法体外建立天冬氨酸酶基因的随机突变库[J].科学通报.1991,19(16):1500-1502.
[4] 林琳,丁文勇,张今.金属离子、蔗糖对天冬氨酸酶活性的影响及其活性部位的分析[J].药物生物技术.1998,5(3):149-152.
[5] Murase S,Yumoto N.Characterization of three types of aspartase activated by site-directed mutagenesis,limited proteolysis,and acetylationl[J].The Journal of Biochemistry,1993,114(5):735-739.
[6] 马林,于振宝,程玉华,等.疏水吸附固定化天冬氨酸酶及其性质的研究[J].生物化学杂志.1991,7(6):671-678.
[7] 周华,杨正景,欧阳平凯.固定化细胞法制备L-天冬氨酸中甲壳素固定化条件的确定[J].南京化工大学学报(自然科学版).1999,21(2):68-70.
[8] 徐礼生,高贵珍,赵亮,等.天冬氨酸酶基因工程菌固定化细胞制备L-天冬氨酸合成条件优化[J].精细化工.2016,33(3):302-306.
[9] 张红缨,李正强,张今,等.酶法体外建立天冬氨酸酶基因的随机突变库[J].科学通报.1991,19(16):1500-1502.
[10] 廖峰,孙小梅,李步海.破壁方法对大肠杆菌AS1.881 中天冬氨酸酶的影响研究[J].氨基酸和生物资源.2004,26(1):29-31.
[11] 张玉彬,王旻,吴梧桐.固定化细胞联产L-苹果酸和L-天冬氨酸的研究[J].中国药科大学学报.1996,27(8):500-503.
[12] 唐芳,谭天伟,王芳.L-天冬氨酸转化菌发酵条件的优化[J].北京化工大学学报.2003,30(5):21-24.
[13] YukawaH,YamadaS,NaraT,et al.Production of L- aspartic acid by reusing cells industrialuse of ethanol utilizing microorganisms[J].Agric Chem Soc Japan.1985,59(1):31-37.
[14] Singh R S,Yadav M.Single-step purification and characterization of recombinant aspartase of Aeromonas media NFB-5[J].Applied Biochemistry & Biotechnology.2012,167(5):991-1001.