干旱胁迫对玫瑰花瓣膜脂过氧化及抗氧化酶活性的影响
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摘要
以‘卡罗拉’(超玫)、‘影星’(艳粉)、‘坦尼克’(白玫)3种云南主栽玫瑰花品种为试材,研究了干旱胁迫对3种玫瑰鲜切花花瓣相对含水量、可溶性蛋白含量、丙二醛含量和抗氧化酶活性的影响,并采用隶属函数法对其抗旱性进行了综合评价。结果表明:(1)随干旱胁迫增强,各品种玫瑰花瓣相对含水量和可溶性蛋白质含量下降,而其代表膜质过氧化程度的丙二醛(MDA)含量不断增加,并在干旱胁迫的第5天达到最大值,且以‘坦尼克’MDA含量最大。(2)随干旱胁迫增强,各品种玫瑰花瓣的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、谷胱甘肽还原酶(GR)以及抗坏血酸过氧化物酶(APX)活性均呈先上升后下降的趋势,其中CAT、POD、GR和APX活性均在第3天时达到最大值,之后CAT、POD、GR活性迅速下降,而APX即使在实验后期也仍然保持较高活性;‘卡罗拉’SOD活性在干旱胁迫下增幅最大,且活性均值也在3个品种中最高,其POD活性在处理第3天时达到最大值,为‘坦尼克’的9.2倍;说明玫瑰花瓣细胞在干旱胁迫第3天时有较高的活性氧(ROS)清除能力,随后细胞抗氧化能力下降并逐渐失去抵抗干旱能力,但APX在玫瑰抵抗干旱胁迫后期起更重要作用,3品种中‘卡罗拉’耐旱性相对较强。(3)根据隶属函数平均值大小对玫瑰花抗旱性进行排序,各玫瑰品种抗旱性由强到弱表现为:‘卡罗拉’>‘影星’>‘坦尼克’,这与干旱胁迫期间各品种花瓣的外观、相对含水量等变化基本相符。综合分析认为,3种玫瑰鲜切花通过提高抗氧化酶活性来增加其抗旱能力,花瓣膜质过氧化程度随着干旱胁迫加重而加剧,且不同品种间表现出不同的抗旱性,并以‘卡罗拉’品种抗旱性较强。
Using the main rose cultivars in Yunnan Province,‘Carola'(red),‘Movie Star'(pink)and‘Tineke'(white)as the experimental materials,we researched the effects of drought stress on the petalsrelative moisture content,soluble protein content,membrane peroxidation and antioxidant enzymeactivities.Our results showed that:(1)relative moisture content and soluble protein content of the pedalsdecreased during the drought stress,while the malondialdehyde(MDA)content kept increasing and reachedthe peak at the fifth day and Tinekes MDA content was the highest.(2)The activities of the petalssuperoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)and glutathione reductase(GR),ascorbic acid peroxidase(APX)declined after rising.CAT,POD,APX and GR reached the maximum on the third day,which illustrated the petal cells had the highest abilities of removing reactive oxygen species(ROS).Then the pedalsantioxidant capacity decreased sharply and the pedals began to senescence and die.The APX activities of three rose varieties did not decline significantly in the later period of drought stress,which indicated that APX played a more important role in drought resistance in the later treatment.The SOD activity of Corolla had the largest increase under the drought stress,and its average activity was also the highest among the three varieties.The POD activity of Corolla reached the maximum at the third day,which was 9.2 times of that of Tineke,indicating that the Corollas drought-tolerant was stronger.(3)According to the average value of membership function,the drought resistance ability of rose is sorted,following as:Carola> Movie Star>Tineke.In conclusion,our results suggest that the three cut rose varieties enhance their drought-tolerant ability by increasing the activities of antioxidant enzymes and the degree of peroxidation of petal membranes is aggravated by drought stress.Different varieties show different drought resistance,especially Carola displays higher drought-tolerant ability.
Using the main rose cultivars in Yunnan Province,‘Carola'(red),‘Movie Star'(pink)and‘Tineke'(white)as the experimental materials,we researched the effects of drought stress on the petalsrelative moisture content,soluble protein content,membrane peroxidation and antioxidant enzymeactivities.Our results showed that:(1)relative moisture content and soluble protein content of the pedalsdecreased during the drought stress,while the malondialdehyde(MDA)content kept increasing and reachedthe peak at the fifth day and Tinekes MDA content was the highest.(2)The activities of the petalssuperoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)and glutathione reductase(GR),ascorbic acid peroxidase(APX)declined after rising.CAT,POD,APX and GR reached the maximum on the third day,which illustrated the petal cells had the highest abilities of removing reactive oxygen species(ROS).Then the pedalsantioxidant capacity decreased sharply and the pedals began to senescence and die.The APX activities of three rose varieties did not decline significantly in the later period of drought stress,which indicated that APX played a more important role in drought resistance in the later treatment.The SOD activity of Corolla had the largest increase under the drought stress,and its average activity was also the highest among the three varieties.The POD activity of Corolla reached the maximum at the third day,which was 9.2 times of that of Tineke,indicating that the Corollas drought-tolerant was stronger.(3)According to the average value of membership function,the drought resistance ability of rose is sorted,following as:Carola> Movie Star>Tineke.In conclusion,our results suggest that the three cut rose varieties enhance their drought-tolerant ability by increasing the activities of antioxidant enzymes and the degree of peroxidation of petal membranes is aggravated by drought stress.Different varieties show different drought resistance,especially Carola displays higher drought-tolerant ability.
引文
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[2]DOORN W G.Water relations of cut flowers[J].Horticulture Review.1997,18:1-85.
[3]YAMAMOTO K,KOMATSU Y,YOKOO Y,et al.Delaying flower opening of cut roses by cis-propenylphosphonic acid[J].Engei Gakkai zasshi,1994,63:159-166.
[4]单宁伟,金基石,赵喜亭,等.切花月季Samantha失水胁迫耐性与其超氧化物歧化酶之间的关联[J].中国农业科学,2005,38(7):1 431-1 438.SHAN N W,JIN J S,ZHAO X T,et al.Relationship between the tolerance to water deficit stress and superoxide dismutases(SODs)in petal of cut rose Samantha[J].Scientia Agricultura Sinica,2005,38(7):1 431-1 438.
[5]金基石,李永红,单宁伟,等.抗坏血酸提高月季切花失水胁迫耐性与增加APX活性的关系[J].园艺学报,2006,33(2):333-337.JIN J S,LI Y H,SHAN N W,et al.Ascorbate acid increases tolerance to water deficit stress in flowers of cut roses(Rosa hybrida L.)caused by enhanced ascorbate peroxi dase activity[J].Acta Horticulture Sinica,2006,33(2):333-337
[6]王子华,金基石,高俊平.谷胱甘肽提高月季切花失水胁迫耐性与GR活性的关系[J].园艺学报,2006,33(1):89-94.WANG Z H,JIN J S,GAO J P.Effect of GSH Pretreatment on the tolerance to water deficit stress in cut rose‘Samantha’and related to enhancement of GR activity[J].Acta Horticulture Sinica,2006,33(1):89-94.
[7]赵喜亭.月季(Rosa hybrida)切花瓶插期间内肽酶与衰老的关系及失水胁迫对其影响的研究[D].陕西杨陵:西北农林科技大学,2002.
[8]张常青,唐雪梅,高俊平,等.切花月季‘萨蔓莎’和‘加布里拉’失水胁迫耐性的差异[J].园艺学报,2002,29(6):556-560.ZHANG C Q,TANG X M,GAO J P,et al.The difference of cut Rosa hybrida‘Samantha’and‘Gabriella’water deficit tolerance[J].Acta Horticulture Sinica,2002,29(6):556-560.
[9]丛日晨,赵喜亭,高俊平.失水胁迫对切花月季‘贝拉米’内肽酶的影响[J].园艺学报,2003,30(3):352-354.CONG R C,ZHAO X T,GAO J P.Effect of water loss stress on the‘Belami’endopeptidase in cut rose[J].Acta Horticulture Sinica,2003,30(3):352-354.
[10]王新悦.切花月季:云南一枝独秀[J].中国花卉园艺,2017,15:10-13.WANG X Y.Cut rose:a single show in Yunnan[J].China Flowers&Horticulture,2017,15:10-13.
[11]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
[12]赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学报,1994,3:207-210.ZHAO S J,XU C C,ZOU Q,et al.Improvements of method for measurement of malonialdehvde in plant tissues[J].Plant Physiology Communications,1994,3:207-210.
[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出出版社,2000:164-169.
[14]NAKANO Y,ASADA K.Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts[J].Plant&Cell Physiology,1981,22,867-880.
[15]GRACE S C,LOGAN B A.Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species[J].Plant Physiology,1996,112:1 631-1 640.
[16]丁龙,赵慧敏,曾文静,等.五种西北旱区植物对干旱胁迫的生理响应[J].应用生态学报,2017,28(5):1 455-1 463.DING L,ZHAO H M,ZENG W J,et al.Physiological responses of five plants in northwest China arid area under drought stress.[J].Chinese Journal of Applied Ecology,2017,28(5):1 455-1 463.
[17]IF GIRON,M CORELL,A GALINDO,et al.Changes in the physiological response between leaves and fruits during a moderate water stress in table olive trees[J].Agricultural Water Management,2015,148:280-286.
[18]HODGES D M,DELONG J M,FOMEY C F,et al.Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds[J].Planta,1999,207:604-611
[19]ELSTNER,E.F.Mechanisms of oxygen activation in different compartments of plant cells[J].Current Topics in Plant Physiology1991,15(10):342-343.
[20]井大炜.杨树苗叶片光合特性和抗氧化酶对干旱胁迫的响应[J].核农学报,2014,28(3):532-539.JING D W.Response of photosynthetic characteristics and antioxidant enzyme activities in poplar seedlings to drought stress[J].Journal of Nuclear Agricultural Sciences,2014,28(3):532-539.
[21]王丹,龚荣高,荣毅.干旱胁迫对枇杷生理特性及生长的影响[J].西北植物学报,2016,36(7):1 399-1 407.WANG D,GONG R G,RONG Y.Physiological characteristics and growth of loquat under drought stress[J].Acta Botanica Boreali-Occidentalia Sinica,2016,36(7):1 399-1 407.
[22]ALSCHER R G.Biosynthesis and antioxidant function of glutathione in plants[J].Physiologia Plantarum,1989,77:457-464.
[23]李伟伟,杜峰,张馨月,等.黄土丘陵区9种群落共存种干旱胁迫下抗氧化酶的响应[J].西北植物学报,2017,37(6):1 145-1 154.LI W W,DU F,ZHANG X Y,et al.Response of reactive oxygen scavenging enzymes among nine co-existing species in Loess Plateau to water deficot[J].Acta Botanica Boreali-Occidentalia Sinica,2017,37(6):1 145-1 154.
[24]龙海涛,李丽梅,谢泽虹,刘帅,李晓云,邓斌,刘海燕,李玲.综合隶属函数法评价花生品种抗旱性与AhNCED1基因表达的关系[J].植物学报,2015,50(6):706-712.LONG H T,LI L M,XIE Z H,et al.Relationship between drought resistance evaluated by comprehensive subordinate function values and AhNCED1expression in peanut varieties[J].Chinese Bulletin of Botany,2015,50(6):706-712.
[25]韩蕊莲,李丽霞,粱宗镇.干旱胁迫下沙棘叶片细胞膜透性与渗透调节物质研究[J].西北植物学报,2003,23(1):23-27.HAN R L,LI L X,LIANG Z Z.Seabuckthorn relative membrane conductivity and osmotic adjustment under drought stress[J].Acta Botanica Boreali-Occidentalia Sinica,2003,23(1):23-27.
[26]陈明涛,赵忠,权金娥.干旱对4种苗木根尖可溶性蛋白组分和含量的影响[J].西北植物学报,2010,30(6):1 157-1 165.CHEN M T,ZHAO Z,QUAN J E.Variation of soluble protein components and contents in seedling root tips of four trees under drought stress[J].Acta Ecologica Sinica,2010,30(6):1 157-1 165.
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